Article
Microbiology
Su-Hyeon Kim, Damilare Emmanuel Adeyemi, Mi-Kyung Park
Summary: KFS-EC3 is a polyvalent and lytic phage that can efficiently infect various bacteria, making it suitable for pathogen control in foods.
Article
Infectious Diseases
Jingyun Zhang, Yuanming Huang, Panpan Xue, Zhifei Zhan, Zheng Huang, Jie Li, Baowei Diao, Biao Kan
Summary: This study evaluated a duplex droplet digital polymerase chain reaction (ddPCR) assay for detecting Salmonella fimY and Shigella ipaH genes. The ddPCR assay showed good linearity and high sensitivity and quantification accuracy for detecting Salmonella and Shigella in stool samples. Compared to quantitative real-time PCR (qPCR), ddPCR detected more positive samples. Salmonella load was higher in diarrheal samples compared to non-diarrheal samples. An optimal cutoff value was determined to distinguish between symptomatic and asymptomatic Salmonella infections.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Food Science & Technology
Eiseul Kim, Chang Hun Choi, Seung-Min Yang, Min-Ki Shin, Hae-Yeong Kim
Summary: To detect Salmonella enterica subsp. enterica serovar Thompson, a new method called droplet digital polymerase chain reaction (ddPCR) was discovered. Compared to the real-time PCR (qPCR) method, ddPCR is more sensitive and rapid, enabling the detection of Salmonella enterica subsp. enterica serovar Thompson at concentrations as low as 1 CFU/25 ml within 90 minutes of pre-enrichment time.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Microbiology
Jie Liu, Suporn Pholwat, Jixian Zhang, Mami Taniuchi, Rashidul Haque, Masud Alam, John Benjamin Ochieng, Jennifer A. Jones, James A. Platts-Mills, Sharon M. Tennant, Eric Houpt
Summary: Real-time PCR assays were evaluated for identifying major serotypes of Shigella flexneri using 0-antigen modification genes. The assays demonstrated high sensitivity and specificity compared to conventional serotyping, with efficient performance on direct stool samples as well. These PCR assays provide a novel tool for S. flexneri serotype identification.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Infectious Diseases
Byron M. Berenger, Linda Chui, Christina Ferrato, Tracie Lloyd, Vincent Li, Dylan R. Pillai
Summary: This study compared the ability of four commercially available RT-PCR tests to detect five major bacterial enteric pathogens. The results showed high concordance rates between RT-PCR and culture for most pathogens, except for Yersinia. Only one of the assays could detect a specific subtype, while none could detect two specific bacterial species. Depending on the prevalence of these pathogens, culture methods may still be critical for their detection.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Multidisciplinary Sciences
Hussein A. Almehdar, Nawal Abd El-Baky, Ehab H. Mattar, Raed Albiheyri, Atif Bamagoos, Abdullah Aljaddawi, Vladimir N. Uversky, Elrashdy M. Redwan
Summary: Camel lactoferrin obtained from different clans of camels in Saudi Arabia exhibits antimicrobial action against S. typhimurium and S. sonnei, and is more effective than bovine and human lactoferrin. It can kill the bacterial strains by iron chelation, affecting bacterial proteins, binding to bacterial LPS and membrane proteins, and disrupting the integrity of bacterial cells and membranes when used alone or in combination with antibiotics.
Article
Food Science & Technology
Wei Guo, Qian Yang, Jie Liu, Xiuling Chen, Yunzhe Zhang, Wei Zhang
Summary: A new MF-SRCA assay was developed for simultaneous detection of Salmonella spp. and Shigella spp. in food. The assay showed high sensitivity and efficiency, with the ability to detect low DNA concentrations and higher positive detection rates compared to other methods.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Microbiology
Roberta Torres de Melo, Taciano dos Reis Cardoso, Phelipe Augusto Borba Martins Peres, Raquelline Figueiredo Braz, Guilherme Paz Monteiro, Daise Aparecida Rossi
Summary: The study analyzed virulence genes, phylogenetic relationships, biofilm formation index, and ultrastructure in S. Minnesota at different temperatures, as well as evaluated its resistance to biocidal agents. Results indicate S. Minnesota has the potential to cause disease in humans and adapt to adverse conditions, especially showing resistance to sodium hypochlorite. Cross-contamination and spread among strains suggest a connection to biofilm formation.
Article
Chemistry, Multidisciplinary
Cheryl S. Y. Yeap, Thanyarat Chaibun, Su Yin Lee, Bin Zhao, Yuan Jan, Chan La-o-vorakiat, Werasak Surareungchai, Shiping Song, Benchaporn Lertanantawong
Summary: The study presents a highly sensitive and selective multiplex assay utilizing an electrochemical DNA sensor and isothermal rolling circle amplification to detect and discriminate three common entero-pathogens in a single reaction. The assay has femtomolar sensitivity and is particularly useful for field- or resource-limited settings.
CHEMICAL COMMUNICATIONS
(2021)
Article
Agriculture, Dairy & Animal Science
Jonathan J. Lopez-Islas, Estela T. Mendez-Olvera, Daniel Martinez-Gomez, Andres M. Lopez-Perez, Libertad Orozco, Gerardo Suzan, Carlos Eslava
Summary: Emerging diseases pose a constant challenge in public health, and wild carnivores can be carriers of atypical strains of pathogenic microorganisms, highlighting the importance of natural environments in studying emerging diseases.
Article
Food Science & Technology
Federica Giacometti, Annalisa Pezzi, Giorgio Galletti, Marco Tamba, Giuseppe Merialdi, Silvia Piva, Andrea Serraino, Silva Rubini
Summary: The study investigated the antimicrobial resistance patterns of Salmonella and E. coli isolates from bivalve molluscs and water samples near Ferrara, Italy, finding high levels of resistance, with most resistant strains showing resistance to multiple antimicrobial agents.
Article
Food Science & Technology
Chanokchon Jaroenporn, Wannakarn Supawasit, Damkerng Bundidamorn, Pathima Udompijitkul, Anunchai Assawamakin, Sudsai Trevanich
Summary: The aim of this study was to validate the developed multiplex PCR-based alternative method for detecting Shiga toxin-producing Escherichia coli (STEC), Listeria monocytogenes (L. monocytogenes), and Salmonella spp. in raw meats. The developed mPCR showed potential as a fast, specific, and sensitive tool for detecting these three bacteria in the raw meat industry.
Article
Multidisciplinary Sciences
Babak Pakbin, Afshin Akhondzadeh Basti, Ali Khanjari, Wolfram Manuel Bruck, Leila Azimi, Abdollah Karimi
Summary: A PCR-HRM assay targeting the rrsA gene was designed and developed in this study to differentiate Shigella species. The assay demonstrated good sensitivity and specificity in distinguishing the Shigella isolates from clinical and food samples, indicating its potential application in public health and food safety surveillance systems.
SCIENTIFIC REPORTS
(2022)
Article
Public, Environmental & Occupational Health
Mysoon M. Al-Ansari, Moneerah M. Aljubali, Ali M. Somily, Ahmad M. Albarrag, Afshan Masood
Summary: The study revealed the presence of Salmonella in chicken meat sold in Riyadh, exhibiting resistance to multiple antibiotics and posing a potential risk of infection to consumers. The genotypic resistance pattern of Salmonella to antibiotics in both human and animal health was highlighted, urging the promotion of antibiotic-free poultry farming as an alternative option.
JOURNAL OF INFECTION AND PUBLIC HEALTH
(2021)
Article
Food Science & Technology
Samir Mohammed Abd-Elghany, Takwa Mohammed Fathy, Amira Ibrahim Zakaria, Kalman Imre, Adriana Morar, Viorel Herman, Raul Pascalau, Laura Smuleac, Doru Morar, Mirela Imre, Khalid Ibrahim Sallam
Summary: This study investigated the presence of Salmonella spp. in buffalo meat in Egypt and studied the antimicrobial susceptibility of the isolated strains. The study found a high level of multidrug-resistant Salmonella contamination in Egyptian buffalo meat, highlighting the need for control measures to prevent Salmonella contamination.
Article
Chemistry, Analytical
Sarah E. Pierce, Courtney L. Sherman, Janarthanan Jayawickramarajah, Candace M. Lawrence, Jonathan L. Sessler, Jennifer S. Brodbelt
ANALYTICA CHIMICA ACTA
(2008)
Article
Biochemistry & Molecular Biology
Sarah E. Pierce, Roxanne Kieltyka, Hanadi F. Sleiman, Jennifer S. Brodbelt
Article
Chemistry, Medicinal
Sarah E. Pierce, Lynn J. Guziec, Frank S. Guziec, Jennifer S. Brodbelt
CHEMICAL RESEARCH IN TOXICOLOGY
(2010)
Article
Chemistry, Multidisciplinary
Sarah E. Pierce, Junmei Wang, Janarthanan Jayawickramarajah, Andrew D. Hamilton, Jennifer S. Brodbelt
CHEMISTRY-A EUROPEAN JOURNAL
(2009)
Article
Physics, Atomic, Molecular & Chemical
Carol Parr, Sarah E. Pierce, Suncerae I. Smith, Jennifer S. Brodbelt
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
(2011)
Article
Biochemical Research Methods
Courtney L. Sherman, Sarah E. Pierce, Jennifer S. Brodbelt, Bodin Tuesuwan, Sean M. Kerwin
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2006)