Review
Multidisciplinary Sciences
Yizhen Fang, Huabin Xie, Chunming Fan
Summary: This study found through meta-analysis that Helicobacter pylori is a significant risk factor for hypertension, with H. pylori-infected individuals having a 13.4% higher risk of developing hypertension compared to uninfected individuals.
Review
Biochemistry & Molecular Biology
Gunter Maubach, Michael Vieth, Francesco Boccellato, Michael Naumann
Summary: NF-03 signaling pathways are crucial for immune response and gastric pathophysiology, and their alterations induced by Helicobacter pylori infection have implications for gastric diseases.
TRENDS IN MOLECULAR MEDICINE
(2022)
Article
Biotechnology & Applied Microbiology
Tanshi Mehrotra, T. Barani Devi, Shakti Kumar, Daizee Talukdar, Sonali Porey Karmakar, Akansha Kothidar, Jyoti Verma, Shashi Kumari, Sneha Mary Alexander, R. J. Retnakumar, Krishnadas Devadas, Animesh Ray, Ankur Mutreja, G. Balakrish Nair, Santanu Chattopadhyay, Bhabatosh Das
Summary: Indian strains of Helicobacter pylori exhibit genome plasticity and heterogeneity, with targeted modifications being the primary mechanism of antibiotic resistance. The findings of this study will enhance understanding of drug-resistant H. pylori emergence and aid in advancing clinical treatment guidance.
Article
Biotechnology & Applied Microbiology
Maria Ines Moreira de Gouveia, Audrey Reuter, Annie Garrivier, Julien Daniel, Annick Bernalier-Donadille, Gregory Jubelin
Summary: Fluorescence-based reporter systems are important tools for studying gene expression dynamics in living cells. However, there are limited strategies to follow gene expression in bacteria within their natural ecosystem. In this study, a plasmid-based tool that combines two different fluorescent proteins as reporter genes was designed and successfully used to monitor gene expression in complex environments. The tool was validated in Escherichia coli and showed the ability to monitor the expression of genes involved in ethanolamine catabolism.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Review
Gastroenterology & Hepatology
Vasiliy Ivanovich Reshetnyak, Alexandr Igorevich Burmistrov, Igor Veniaminovich Maev
Summary: This review summarizes the role of Helicobacter pylori in gastrointestinal diseases, exploring both potential benefits and risks to human health. There is significant controversy in the current literature regarding the impact of H. pylori on human health.
WORLD JOURNAL OF GASTROENTEROLOGY
(2021)
Article
Immunology
Dongjie Fan, Yanan Gong, Lu Sun, Yiyao Zhang, Jianzhong Zhang
Summary: The study revealed that high concentrations of zinc exert antimicrobial activity against Helicobacter pylori by repressing or altering multiple pathogenetic genes and metabolic pathways associated with bacteria growth. This finding has significant implications for understanding the antimicrobial activity mechanism of zinc and the role of zinc-mediated homeostasis in Helicobacter pylori.
MICROBIAL PATHOGENESIS
(2022)
Article
Agriculture, Multidisciplinary
Zhi-hao Yu, Mei Cao, Yuan-xiao Wang, Shi-ying Yan, Li-ting Qing, Cheng-meng Wu, Shu Li, Tian-yi Li, Qian Chen, Jian Zhao
Summary: The study found that Urolithin A (UA) effectively attenuates the inflammation caused by Helicobacter pylori (H. pylori) and reduces tissue damage in mice. In addition, UA decreases the relative abundance of Helicobacteraceae in the feces of H. pylori-infected mice.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Mateusz Noszka, Agnieszka Strzalka, Jakub Muraszko, Rafal Kolenda, Chen Meng, Christina Ludwig, Kerstin Stingl, Anna Zawilak-Pawlik
Summary: This study presents a comprehensive investigation of the redox switch protein HP1021 regulon in the gastric human pathogen Helicobacter pylori. The findings suggest that HP1021 plays a crucial role in modulating the response to oxidative stress in H. pylori, controlling the transcription of numerous genes and influencing the expression of proteins involved in various pathways. Additionally, HP1021 is found to regulate competence and glucose consumption, impacting the energetic balance in the cell.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Pragyesh Dixit, Shrikant B. Kokate, Indrajit Poirah, Debashish Chakraborty, Duane T. Smoot, Hassan Ashktorab, Niranjan Rout, Shivaram P. Singh, Asima Bhattacharyya
Summary: H. pylori infection induces phosphorylation of Siah2 in gastric epithelial cells, leading to increased tumorigenicity. The phosphorylation of Siah2 by MRCK beta stabilizes Siah2 and enhances the proliferative and invasive capabilities of infected cells.
JOURNAL OF BIOMEDICAL SCIENCE
(2021)
Article
Biology
Florent Ailloud, Wilhelm Gottschall, Sebastian Suerbaum
Summary: An extensive dataset of Helicobacter pylori methylome suggests that natural selection can regulate the epigenetic landscape by configuring methyltransferases and their recognition sequences. The frequency and abundance of methyltransferases and their recognition sequences were influenced by phylogeographic structure, with some type II methyltransferases showing positive or negative correlation. The study also revealed that methylation patterns can be enriched by positive selection and specific sites have important functions in methylation-dependent phenotypes.
COMMUNICATIONS BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ruijia Yang, Jiajia Li, Jingru Wang, Yufan Wang, Fengwang Ma, Rui Zhai, Pengmin Li
Summary: This study evaluated the anti-Helicobacter pylori capacity of natural products from Winter Red crabapple flowers and found that kaempferol showed the highest antibacterial capacity. The antibacterial mechanism of kaempferol was distinct from the antibiotics clarithromycin and amoxicillin.
JOURNAL OF FOOD BIOCHEMISTRY
(2022)
Review
Cell Biology
Hang Yang, Bing Hu
Summary: Helicobacter pylori is a gram-negative bacterium that causes gastritis and gastric cancer. Its infection is mainly transmitted through oral-oral and fecal-oral routes, usually occurring in early childhood. H. pylori achieves persistent colonization by manipulating the host immune responses, leading to mucosal damage and inflammation. Understanding the mechanisms and characteristics of chronic inflammation caused by H. pylori is important for understanding H. pylori gastritis.
MEDIATORS OF INFLAMMATION
(2022)
Article
Biochemistry & Molecular Biology
Carly Boye, Sezgi Arpag, Michael Francis, Scott DeClemente, Aislin West, Richard Heller, Anna Bulysheva
Summary: Gene therapy has a wide range of applications but viral delivery methods have safety concerns. By using smaller plasmid DNA backbones, gene expression levels can be significantly increased using non-viral methods, as shown in rat tenocytes and myocardium. Delivery to the skin showed more moderate improvements.
BIOELECTROCHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Yang Wu, Zhiyong Song, Guiyun Deng, Kai Jiang, Huajuan Wang, Xueji Zhang, Heyou Han
Summary: The study uses nanomotors loaded with drugs to combat gastric cancer caused by Helicobacter pylori infection, successfully achieving rapid neutralization of gastric acid and drug release in animal models to achieve good therapeutic effects.
Article
Microbiology
Maia J. Baskerville, Yekaterina Kovalyova, Raquel Mejias-Luque, Markus Gerhard, Stavroula K. Hatzios
Summary: The gastric pathogen Helicobacter pylori decreases host glutathione levels and utilizes the degraded products for nutritional gain during infection. This depletion of glutathione is driven by the bacterial enzyme & gamma;-glutamyl transpeptidase and occurs through an oxidation-independent mechanism.
Article
Biochemistry & Molecular Biology
Barbara Bortot, Maura Apollonio, Gabriele Baj, Laura Andolfi, Luisa Zupin, Sergio Crovella, Matteo di Giosia, Andrea Cantelli, Roberto Saporetti, Luca Ulfo, Annapaola Petrosino, Giovanni Di Lorenzo, Federico Romano, Giuseppe Ricci, Maurizio Mongiat, Alberto Danielli, Matteo Calvaresi, Stefania Biffi
Summary: This study proposes a bacteriophage-based receptor targeted photodynamic therapy for treating EGFR-positive ovarian cancer. The modified M13 bacteriophage with an EGFR binding peptide displayed on its surface effectively kills cancer cells through ROS generation by photosensitizers. It also downregulates EGFR expression and induces autophagy.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Chemistry, Multidisciplinary
Luca Ulfo, Andrea Cantelli, Annapaola Petrosino, Paolo Emidio Costantini, Michela Nigro, Francesco Starinieri, Eleonora Turrini, Suleman Khan Zadran, Giampaolo Zuccheri, Roberto Saporetti, Matteo Di Giosia, Alberto Danielli, Matteo Calvaresi
Summary: Using an orthogonal nanoarchitectonics approach, M13 bacteriophages were engineered to specifically target cancer cells with EGFR overexpression, and efficiently deliver photosensitizing molecules for photodynamic therapy at picomolar concentrations.
Review
Pharmacology & Pharmacy
Luca Ulfo, Paolo Emidio Costantini, Matteo Di Giosia, Alberto Danielli, Matteo Calvaresi
Summary: EGFR-targeted photodynamic therapy is a promising approach for cancer treatment, which selectively delivers photosensitizers to tumor environments. This review summarizes recent nanobiotechnological advancements combining photodynamic therapy with EGFR-targeted molecular cancer therapy, highlighting its advantages, drawbacks, and future perspectives.
Article
Microbiology
Davide Roncarati, Vincenzo Scarlato, Andrea Vannini
Summary: Since the discovery of penicillin, antibiotics have played a crucial role in fighting bacterial infections but the rise of antibiotic-resistant pathogens has made it increasingly difficult to treat these infections. This article highlights the potential of bacterial transcriptional and posttranscriptional regulators as targets for developing new drugs and discusses recent advances in finding compounds that can inhibit their biological activity.
Article
Biochemistry & Molecular Biology
Gerardo Della Sala, Consiglia Pacelli, Francesca Agriesti, Ilaria Laurenzana, Francesco Tucci, Mirko Tamma, Nazzareno Capitanio, Claudia Piccoli
Summary: This study characterizes the metabolic profiles of osteosarcoma-derived cell lines and reveals the reliance of cancer stem cells on glycolysis and differentiated cells on glutamine oxidation. The results demonstrate that the efficacy of metabolite starvation combined with chemotherapy depends on the specific cancer compartment, highlighting the need for caution in using this strategy.
Article
Multidisciplinary Sciences
Sonia Nicchi, Fabiola Giusti, Stefano Carello, Sabrina Utrio Lanfaloni, Simona Tavarini, Elisabetta Frigimelica, Ilaria Ferlenghi, Silvia Rossi Paccani, Marcello Merola, Isabel Delany, Vincenzo Scarlato, Domenico Maione, Cecilia Brettoni
Summary: Moraxella catarrhalis and nontypeable Haemophilus influenzae (NTHi) are both pathogens associated with exacerbation of chronic obstructive pulmonary disease (COPD). This study found that Moraxella catarrhalis induces less reactive oxygen species (ROS) and neutrophil extracellular traps (NET) compared to NTHi. It also interferes with cellular responses in a unique manner, interfering with autophagy and promoting the survival of other bacteria that are susceptible to host antimicrobial defenses.
Article
Microbiology
Sara Borghi, Ana Antunes, Andreas F. Haag, Marco Spinsanti, Tarcisio Brignoli, Enea Ndoni, Vincenzo Scarlato, Isabel Delany
Summary: NHBA is a surface-exposed lipoprotein expressed by Neisseria meningitidis, and its expression is controlled by temperature. Lower temperatures result in higher NHBA expression, leading to increased surface exposure. This temperature-regulated expression might be relevant during transmission and the initial interaction within the host nasopharynx.
Article
Biochemistry & Molecular Biology
Federico Antoniciello, Davide Roncarati, Annamaria Zannoni, Elena Chiti, Vincenzo Scarlato, Federica Chiappori
Summary: Helicobacter pylori is a widespread and antibiotic-resistant bacterial pathogen. Researchers have used computational and experimental methods to identify several drugs that can inhibit the binding of its transcription factor to DNA, providing promising options for developing new drugs against H. pylori.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Andrea Vannini, Davide Roncarati, Federico D'Agostino, Federico Antoniciello, Vincenzo Scarlato
Summary: Bacterial pathogens like H.pylori employ regulatory systems to coordinate virulence gene expression, and understanding the molecular mechanisms of gene transcription regulation is crucial in this process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Giulia Greco, Luca Ulfo, Eleonora Turrini, Alessia Marconi, Paolo Emidio Costantini, Tainah Dorina Marforio, Edoardo Jun Mattioli, Matteo Di Giosia, Alberto Danielli, Carmela Fimognari, Matteo Calvaresi
Summary: Combining photodynamic therapy with chemotherapy can enhance the anticancer effects of the drug and reduce its dose-related adverse effects. Doxorubicin, a successful chemotherapy drug, has intrinsic photosensitizing properties that can be exploited to enhance its anticancer activity.
Article
Biochemistry & Molecular Biology
Matteo Micucci, Anna Stella Bartoletti, Fuad O. Abdullah, Sabrina Burattini, Ilaria Versari, Matteo Canale, Federico D'Agostino, Davide Roncarati, Diletta Piatti, Gianni Sagratini, Giovanni Caprioli, Michele Mari, Michele Retini, Irene Faenza, Michela Battistelli, Sara Salucci
Summary: This study aimed to investigate the chemical composition and evaluate the antibacterial and antitumor activities of Aristolochia olivieri Colleg. ex Boiss. Leaves' methanolic extract (AOME). The results showed that AOME exhibited significant antimicrobial effects against Helicobacter pylori and reduced cell viability in gastric cancer cell lines, potentially through inducing cellular apoptosis. These findings suggest that AOME holds promise as a natural resource for innovative approaches in gastric cancer management.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Medicinal
Manuele Di Sante, Alena Kaltenbrunner, Marco Lombardo, Alberto Danielli, Paolo Emidio Costantini, Matteo Di Giosia, Matteo Calvaresi
Summary: A Ce6-C-60 dyad was synthesized, which showed improved cellular uptake of Ce6 and enhanced generation of reactive oxygen species (ROS). It exhibited higher PDT efficacy than Ce6 alone, making it promising for photodynamic therapy.
Article
Chemistry, Multidisciplinary
Giulia Battistelli, Maria Proetto, Alexandra Mavridi-Printezi, Matteo Calvaresi, Alberto Danielli, Paolo Emidio Constantini, Claudia Battistella, Nathan C. Gianneschi, Marco Montalti
Summary: Fluorogenic nanoparticles that can respond to different physiological environments and differentially release drugs have great potential in nanomedicine. pH-responsive NPs, especially, have the ability to distinguish cancer tissues, release drugs selectively, and act as pH biosensors. The assembly of ROMP block-copolymers provides a solid route for the design and synthesis of pH-responsive fluorogenic micelles. This approach shows promise in the development of novel nano-theranostic agents.
