Article
Agriculture, Dairy & Animal Science
Xiuxiu Miao, Ya'nan Zhao, Huilong Li, Yanru Ren, Geng Hu, Jingchao Yang, Liying Liu, Xianyao Li
Summary: Salmonella enterica serovar Enteritidis is a common pathogen that can cause significant public health problems and economic losses. This study investigated the genetic resistance of poultry to Salmonella infection and found that inflammatory genes were activated in chickens infected with Salmonella Enteritidis. The findings provide important insights into the molecular mechanisms and epigenetic regulation in chicken response to Salmonella infection.
Article
Immunology
Michael H. Kogut, Kenneth J. Genovese, J. Allen Byrd, Christina L. Swaggerty, Haiqi He, Yuhua Farnell, Ryan J. Arsenault
Summary: Poultry is a major source of foodborne illnesses caused by Salmonella, and understanding the phosphorylation-based cellular signaling responses can provide valuable insights into the host's early interactions with the pathogen. Through kinome analysis, it was found that infection with S. Enteritidis led to increased innate immune response signaling pathways and alterations in JAK-STAT pathway phosphorylation events, indicating the initiation of host defense mechanisms against Salmonella infection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
De Wang, Boheng Ma, Ziwei Liao, Wenjing Li, Tiejun Zhang, Changwei Lei, Hongning Wang
Summary: Flaxseed supplementation has a inhibitory effect on Salmonella infection in laying hens, reducing colonization and accelerating clearance of Salmonella. It also protects the infected intestinal and ovarian tissues. Additionally, flaxseed supplementation increases the abundance of beneficial intestinal bacteria.
Article
Veterinary Sciences
Xiuxiu Miao, Lewen Liu, Liying Liu, Geng Hu, Guixian Wu, Yuanmei Wang, Yanan Zhao, Jingchao Yang, Xianyao Li
Summary: By analyzing the mRNA and miRNA expression profiles of the cecum of Shouguang chickens, it was found that there were significant differences in the expression of mRNA and miRNA between the SE-infected group and the control group. The differentially expressed mRNAs were mainly enriched in immune-related processes and KEGG pathways, while the differentially expressed miRNAs were mainly enriched in metabolic-related GO-BP terms and KEGG pathways. Further experiments showed that miR-20b-5p regulates the immune or metabolic responses in Shouguang chickens after SE infection by directly targeting SCNN1A.
BMC VETERINARY RESEARCH
(2022)
Article
Agriculture, Dairy & Animal Science
Shu Wu, Qianyun Zhang, Guanglei Cong, Yunqi Xiao, Yiru Shen, Shan Zhang, Wenchang Zhao, Shourong Shi
Summary: This study investigated the anti-Salmonella Enteritidis effects of probiotic Escherichia coli Nissle 1917 in chicks. The results showed that gavage of E. coli Nissle 1917 reduced the infection of S. Enteritidis, improved intestinal morphology and barrier function, and altered cecum microbiota composition. These findings suggest that E. coli Nissle 1917 has enormous potential as an alternative to antibiotics.
Article
Agronomy
Xiaoqing Hu, Xiaoying Sun, Shuanghua Luo, Shuyan Wu, Zhaojuan Chu, Xiujuan Zhang, Zhaojun Liu, Jiaxin Wu, Xiaohong Wang, Chang Liu, Xiaoyuan Wang
Summary: The study demonstrated the effectiveness of blue light (BL) in inactivating Salmonella Enteritidis on eggshells, while maintaining eggshell freshness. BL-treated eggs showed lower weight loss, higher Haugh unit, and higher total amino acid content during storage period, indicating the advantage of BL treatment in maintaining egg quality.
Article
Biotechnology & Applied Microbiology
Xueran Mei, Boheng Ma, Xiwen Zhai, Anyun Zhang, Changwei Lei, Lei Zuo, Xin Yang, Changyu Zhou, Hongning Wang
Summary: Florfenicol administration significantly increased the loads of S. Enteritidis in cecal contents, spleen, and liver in neonatal chickens, affecting cecal colony structures and linoleic acid metabolism. The altered microbiota and metabolome by florfenicol mainly promote Salmonella infection through linoleic acid metabolism.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biology
Sion C. Bayliss, Rebecca K. Locke, Claire Jenkins, Marie Anne Chattaway, Timothy J. Dallman, Lauren A. Cowley, Ben S. Cooper
Summary: This study developed a hierarchical machine learning model to rapidly identify and trace the geographical source of Salmonella enterica serovar Enteritidis infections from whole genome sequencing data. The model achieved high accuracy in predicting the continent, sub-region, and country of origin, and provides results within 4 minutes per sample, facilitating rapid outbreak resolution and real-time genomic epidemiology.
Article
Infectious Diseases
Elena Circella, Gaia Casalino, Francesco D'Amico, Nicola Pugliese, Michela Maria Dimuccio, Antonio Camarda, Giancarlo Bozzo
Summary: In recent years, there has been a more cautious use of drugs on livestock farms, leading to the consideration of alternative products in order to reduce the risk of antibiotic resistance. This study aimed to evaluate the effectiveness of garlic in inhibiting Salmonella enterica subsp. enterica serovar Enteritidis and to determine its Minimal Inhibitory Concentration (MIC)(90) and MIC50 values. The results showed that garlic has the potential to inhibit the growth of S. Enteritidis in vitro, with efficacy varying depending on the microbial concentration used.
Article
Agriculture, Dairy & Animal Science
Xin Yu, Hongwei Zhu, Yongheng Bo, Youzhi Li, Yue Zhang, Yang Liu, Jianlong Zhang, Linlin Jiang, Guozhong Chen, Xingxiao Zhang
Summary: The study investigated the prevalence and antimicrobial resistance of Salmonella enterica serovar Enteritidis in broiler chickens in Shandong Province, China. It found that S. Enteritidis was the predominant serovar with high antimicrobial resistance rates to several antibiotics. Multidrug resistance, particularly to fluoroquinolone, showed an increasing trend from 2013 to 2018. This study provides updated information on antimicrobial resistance data and risk assessment for Salmonella Enteritidis in broiler chickens in Shandong Province, which can be valuable for future surveillance activities to ensure chicken supply safety.
Article
Microbiology
Xi Wang, Xiangju Wu, Xiaoyan Cong, Jinrui Ren, Juntong Li, Jianshen Zhu, Meixue Dai, Natalia Hrabchenko, Yijun Du, Jing Qi
Summary: This study found that fecal microbiota transplantation (FMT) from adult SPF chickens could reduce Salmonella Enteritidis (SE) infection in chicks by improving their growth and immune response, as well as inhibiting SE colonization in the liver. FMT also regulated the intestinal microbiota of the chicks.
VETERINARY MICROBIOLOGY
(2022)
Article
Microbiology
Geng Hu, Liying Liu, Xiuxiu Miao, Yanan Zhao, Yanan Peng, Lewen Liu, Xianyao Li
Summary: This study investigated the response of the cecal microbiota to an inflammatory state caused by Salmonella enterica serovar Enteritidis infection. It was found that the infection improved microbial diversity and promoted microbiota evolution. Some intestinal microorganisms were found to thrive in an inflamed environment, while the overall function of the cecal microbiome was to maintain homeostasis through the up-regulation of microbial metabolism pathways. The infection of S. Enteritidis was closely related to multiple inflammation-related genes and S. Enteritidis was able to compete with symbiotic bacteria.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Christina L. Swaggerty, Ramon D. Malheiros, Ludovic Lahaye, Hector H. Salgado, J. Allen Byrd II, Kenneth J. Genovese, Haiqi He, Elizabeth Santin, Michael H. Kogut
Summary: The study aimed to determine whether feeding a complex of protected biofactors and antioxidants to broiler breeder hens could increase resistance against Salmonella infection in their offspring. The results showed that chicks from hens fed with the supplement had lower rates of Salmonella detection and colonization. Furthermore, there were differences in the production of cytokines and chemokines between the two groups of chicks.
Article
Veterinary Sciences
Luisa D'Angelo, Rubina Paradiso, Domenico Alfano, Marita Georgia Riccardi, Giorgia Borriello, Giorgio Galiero
Summary: This study describes the first detection of Salmonella enterica subsp. enterica Serovar Enteritidis in an aborted water buffalo fetus in southern Italy, providing significant insights into the role of S. Enteritidis in causing abortion in water buffalo.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Immunology
Yumin Xu, Ahmed G. Abdelhamid, Anice Sabag-Daigle, Michael G. Sovic, Brian M. M. Ahmer, Ahmed E. Yousef
Summary: The contribution of food vehicles to the pathogenicity of disease-causing microorganisms, specifically Salmonella enterica serovar Enteritidis, has been revealed in a study. The research shows that the growth of Salmonella Enteritidis in egg yolk enhances its virulence compared to growth in tryptic soy broth. This finding is supported by transcriptional analysis results, which indicate the upregulation of virulence-related genes in Salmonella Enteritidis grown in egg yolk. These findings highlight the significance of egg yolk as a medium for the risk assessment of salmonellosis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Agriculture, Dairy & Animal Science
Ivan Rychlik
Article
Microbiology
Zuzana Seidlerova, Tereza Kubasova, Marcela Faldynova, Magdalena Crhanova, Daniela Karasova, Vladimir Babak, Ivan Rychlik
Article
Veterinary Sciences
Daniela Karasova, Magdalena Crhanova, Vladimir Babak, Martin Jerabek, Lubos Brzobohaty, Zuzana Matesova, Ivan Rychlik
Summary: The study revealed that certain microbial populations before weaning may increase the risk of piglets developing diarrhea, while other microbial populations after weaning may enhance piglet health and disease resistance. Therefore, changes in microbiota before and after weaning are crucial for piglet resistance to postweaning diarrhea under field conditions.
RESEARCH IN VETERINARY SCIENCE
(2021)
Article
Multidisciplinary Sciences
Helena Juricova, Jitka Matiasovicova, Tereza Kubasova, Darina Cejkova, Ivan Rychlik
Summary: This study investigated the presence of horizontally acquired antibiotic resistance genes among chicken gut microbiota and found that genes coding for resistance to tetracyclines, macrolide-lincosamide-streptogramin B antibiotics, and aminoglycosides were the most common. The study also showed that antibiotic resistance is quite common in gut commensals, with certain bacterial families serving as reservoirs for these resistances.
SCIENTIFIC REPORTS
(2021)
Review
Biochemistry & Molecular Biology
Tereza Kubasova, Zuzana Seidlerova, Ivan Rychlik
Summary: The ecological adaptations of gut microbiota members play a crucial role in their potential as probiotics, with a relationship between aerobic survival and abundance or potential for prolonged colonization in the intestinal tract. To maximize probiotic efficacy, their mode of administration should reflect their natural ecology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Jiri Volf, Magdalena Crhanova, Daniela Karasova, Marcela Faldynova, Tereza Kubasova, Zuzana Seidlerova, Alena Sebkova, Michal Zeman, Helena Juricova, Jitka Matiasovicova, Marian Foltyn, Zdenek Tvrdon, Ivan Rychlik
Summary: This study investigated the origin of chicken gut microbiota in commercial production through microbiota analysis by 16S rRNA sequencing. It was found that neither eggshells nor feed served as major sources of gut microbiota for newly hatched chickens, and they must be colonised from additional sources such as air dust with spores of Clostridiales.
Correction
Biotechnology & Applied Microbiology
Robert Dixon, Stephen G. Preston, Stefan Dascalu, Patrik G. Flammer, Steven R. Fiddaman, Kirstie McLoughlin, Amy Boyd, Jiri Volf, Ivan Rychlik, Michael B. Bonsall, Bernd Kaspers, Adrian L. Smith
Article
Biotechnology & Applied Microbiology
Robert Dixon, Stephen G. Preston, Stefan Dascalu, Patrik G. Flammer, Steven R. Fiddaman, Kirstie McLoughlin, Amy Boyd, Jiri Volf, Ivan Rychlik, Michael B. Bonsall, Bernd Kaspers, Adrian L. Smith
Summary: This study reveals that chicken TCR gamma locus has undergone tandem duplication events that have shaped its evolution. Repertoire sequencing shows large differences in the usage of individual TRGV genes across various tissues, with TRGV3.3 dominating every tissue. The application of repertoire analysis in chickens with a high circulating gamma delta phenotype is important for understanding the functional diversity of these cells and their potential in improving health.
Article
Gastroenterology & Hepatology
Jana Schreier, Daniela Karasova, Magdalena Crhanova, Ivan Rychlik, Silke Rautenschlein, Arne Jung
Summary: This study confirmed the effectiveness of early lincomycin-spectinomycin treatment during the first week of life in preventing EC-associated disease in broiler type chickens and its impact on the development of the cecal microbiota. Regardless of EC-infection, lincomycin-spectinomycin promoted the growth of Escherichia/Shigella and reduced the amount of Lactobacillus spp. in the ceca. Unexpectedly, the highest abundances of Enterococcus were found after antibiotic treatment, suggesting the growth of resistant enterococcal species. EC was not detected among the most abundant members of the genus Enterococcus. Oral EC-infection at the first day of life did not influence the development of cecal microbiota.
Article
Agriculture, Dairy & Animal Science
Jiri Volf, Jana Rajova, Vladimir Babak, Zuzana Seidlerova, Ivan Rychlik
Summary: The ileum and caecum sections of the chicken gut exhibit distinct differences in function and protein expression, with the ileum containing proteins related to structure and nutrient absorption, while the caecum harbors proteins associated with oxidative stress response and wound healing. Additionally, the caecum shows a higher expression of proteins involved in hydrogen sulphide metabolism and the activation of mechanisms to suppress inflammatory responses.
Article
Microbiology
Helena Juricova, Jitka Matiasovicova, Marcela Faldynova, Alena Sebkova, Tereza Kubasova, Hana Prikrylova, Daniela Karasova, Magdalena Crhanova, Hana Havlickova, Ivan Rychlik
Summary: The colonization efficiency and anti-infection effect of lactobacilli in the chicken intestinal tract are not significant. However, this study found that a mixture of lactobacilli and Salmonella Enteritidis fermentation can protect chickens against infection by acidifying the feed and inactivating the bacteria.
Article
Microbiology
Daniela Karasova, Marcela Faldynova, Jitka Matiasovicova, Alena Sebkova, Magdalena Crhanova, Tereza Kubasova, Zuzana Seidlerova, Hana Prikrylova, Jiri Volf, Michal Zeman, Vladimir Babak, Helena Juricova, Jana Rajova, Lenka Vlasatikova, Petr Rysavka, Ivan Rychlik
Summary: The gut microbiota of warm-blooded vertebrates consists mainly of bacterial species belonging to the phyla Firmicutes and Bacteroidetes. Species capable of survival in an aerobic environment are commonly found in humans, pigs, chickens, and penguins, while gut anaerobes without specific adaptation for aerobic survival exhibit host adaptation.
Article
Gastroenterology & Hepatology
Janina Rzeznitzeck, Gerhard Breves, Ivan Rychlik, Frederic J. Hoerr, Alexandra von Altrock, Alexandra Rath, Silke Rautenschlein
Summary: Despite successful colonization, the turkeys showed no clinical signs or pathological lesions. C. jejuni affected gut health and may interfere with turkey productivity, while the impact of C. coli on gut health was less pronounced. Interestingly, co-inoculated animals showed less affected gut morphology and functional integrity, suggesting C. coli may outcompete C. jejuni.
Article
Microbiology
Stanislava Kralova, Lenka Davidova-Gerzova, Adam Valcek, Matej Bezdicek, Ivan Rychlik, Veronika Rezacova, Alois Cizek
Summary: This study analyzed three difficult-to-cultivate and strictly anaerobic strains and proposed classifying them into two new genera.
MICROBIOLOGY SPECTRUM
(2022)
Article
Veterinary Sciences
Mohamed Kamal Abdelhamid, Ivan Rychlik, Claudia Hess, Tamas Hatfaludi, Magdalena Crhanova, Daniela Karasova, Julia Lagler, Dieter Liebhart, Michael Hess, Surya Paudel
Summary: This study investigated the dynamics of the gut microbiome in turkeys following experimental infection with Histomonas meleagridis. Results showed that the infection led to a fundamental shift in the microbial community, particularly affecting the relative abundance of Escherichia coli without a significant impact on their absolute count. Additionally, E. coli cells did not show a tendency to penetrate the caecal tissue even with severely compromised mucosal barriers.
VETERINARY RESEARCH
(2021)
