4.6 Article

Synthesis of Chiral Cyanohydrins by Recombinant Escherichia coli Cells in a Micro-Aqueous Reaction System

Journal

APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 78, Issue 14, Pages 5025-5027

Publisher

AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.00582-12

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Funding

  1. DFG research training group [GK1166]

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Synthesis of chiral cyanohydrins is performed in a monophasic micro-aqueous reaction system using whole recombinant Escherichia coli cells expressing the Arabidopsis thaliana hydroxynitrile lyase (AtHNL). Microscopy studies employing a fusion of AtHNL with a flavin-based fluorescent protein (FbFP) reveal that the cells remain intact in the reaction system.

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