Article
Materials Science, Paper & Wood
Shanshan Wu, Xiao Jiang, Huicong Jiang, Shufang Wu, Shaojun Ding, Yongcan Jin
Summary: The study found that the crystallinity of cotton linter pulp has the most significant impact on the processivity of EG1 enzyme, followed by the initial cellulose-reducing ends. The degree of polymerization (DP) indirectly affects enzymatic hydrolysis, influenced by the pulp preparation method and conditions.
Article
Biochemistry & Molecular Biology
Laure Ayme, Agnes Hebert, Bernard Henrissat, Vincent Lombard, Nathalie Franche, Stephanie Perret, Etienne Jourdier, Senta Heiss-Blanquet
Summary: The study identified six GH9 enzymes from a compost metagenome and characterized three of them, revealing their diverse structures and functional characteristics. These enzymes play a crucial role in bacterial biomass deconstruction.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2021)
Article
Biochemistry & Molecular Biology
Ardhendu Mandal, Abhijeet Thakur, Arun Goyal
Summary: This study investigated the biochemical properties of a recombinant 8-1,4 endoglucanase, AtGH9C-CBM3A-CBM3B, and the role of its associated CBMs in catalysis. The enzyme showed maximum activity at 55°C and pH 7.5, and exhibited high activity against carboxy methyl cellulose, lichenan, 8-glucan, and hydroxy ethyl cellulose. The catalytic module, AtGH9C, showed insignificant activity, emphasizing the essential requirement of CBMs in catalysis. The associated CBMs also contributed to thermostability. The results highlight the importance of the physical association and cross-talk between AtGH9C and its associated CBMs in efficient cellulose catalysis.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Parmeshwar Vitthal Gavande, Krishan Kumar, Jebin Ahmed, Arun Goyal
Summary: In this study, the multifunctional endoglucanase RfGH5_4 from Ruminococcus flavefaciens was found to have a (beta/alpha)8-TIM barrel structure through homology modeling. Experimental analyses confirmed the predicted secondary structure of RfGH5_4 and molecular dynamic simulation revealed the stability of its structural fold when binding to a cellulosic ligand. RfGH5_4 showed strong affinity towards cellulosic and hemicellulosic ligands, suggesting its potential in various industrial applications. The study also highlighted the role of specific loops in the catalytic cleft of RfGH5_4 and its monodispersed state and stability.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Jiumn-Yih Wu, Tzi-Yuan Wang, Hsiou-Yu Ding, Yun-Rong Zhang, Shu-Yuan Lin, Te-Sheng Chang
Summary: Researchers found that an enzyme from Bacillus thuringiensis could convert vitexin into two new compounds with higher aqueous solubility, which enhances the potential applications of vitexin.
Article
Chemistry, Physical
Jorick Franceus, Jolien Lormans, Lore Cools, Matthias D'hooghe, Tom Desmet
Summary: The study demonstrates significant phosphorylase activity can be established in GH3 glycoside hydrolases through single-site substitutions and further optimized through mutational paths. These findings suggest new insights into developing phosphorylases and provide a novel approach for future research in this field.
Review
Biotechnology & Applied Microbiology
Tomohiko Matsuzawa, Akira Watanabe, Takahiro Shintani, Katsuya Gomi, Katsuro Yaoi
Summary: Aspergillus species are closely associated with humanity through fermentation, infectious disease, and mycotoxin contamination of food. This review focuses on the machinery of the xyloglucan degradation in Aspergillus species using glycoside hydrolases, emphasizing the crucial role of cooperative actions of these enzymes.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Aditi Konar, Shritama Aich, Ranaprathap Katakojwala, Supratim Datta, S. Venkata Mohan
Summary: This study focuses on GH9 endoglucanases and identifies an highly active enzyme, H1AD14, from Bacillus licheniformis. The enzyme shows high enzymatic activity on various substrates and exhibits salt tolerance and processivity. The findings contribute to the understanding of GH9 endoglucanases and offer potential for developing improved cellulase cocktails for biomass deconstruction.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Fei Zheng, Huanxi Zhao, Nan Wang, Peng Zhong, Kailu Zhou, Shanshan Yu
Summary: This study reported for the first time a thermophilic endoglucanase belonging to the GH5 family, which showed high selectivity for cleaving specific glucose residues of ginsenosides and produced compounds with stronger pharmacological activity.
Article
Biochemistry & Molecular Biology
Casey Crooks, Nathan J. Bechle, Franz J. St John
Summary: This study characterized a non-reducing terminal (NRT)-specific xylobiohydrolase AcXbh30A, which strictly cleaves xylobiose from the NRT with minimal other hydrolysis products under optimized reaction conditions. This new xylobiohydrolase may be useful for commercial production of industrial quantities of xylobiose.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Raja Bhaiyya, Sukesh Chander Sharma, Ravindra Pal Singh
Summary: Human consumption of larch arabinogalactan enhances probiotic microflora and promotes the production of short-chain fatty acids in the gut. This study explores the biochemistry of a bacterial member of the Lachnospiraceae family, B. producta ATCC 27340, and identifies an enzyme (Bp0469) that plays a role in utilizing non-cellulosic fiber in the gut. The findings contribute to understanding the nutritional perspective of B. producta ATCC 27340 and its potential industrial applications in producing important arabinogalactan oligosaccharides (AGOs).
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Biochemical Research Methods
Evan Glasgow, Kirk Vander Meulen, Nate Kuch, Brian G. Fox
Summary: Enzyme performance is crucial for the bioeconomy based on renewable plant materials, especially multifunctional cellulases (MFCs) which play a key role in efficiently hydrolyzing plant biomass into sugars. Recent research has revealed novel characteristics of MFCs, such as broad substrate specificity and intramolecular synergy. Utilizing binding modules and accessory catalytic domains can amplify enzyme activity, while multidomain MFCs from Caldicellulosiruptor show potential for recombinant design.
CURRENT OPINION IN BIOTECHNOLOGY
(2021)
Article
Chemistry, Medicinal
Dianyi Li, Zheng Wang, Yong Yu, Huirong Li, Wei Luo, Bo Chen, Guoqing Niu, Haitao Ding
Summary: A novel GH2 beta-galactosidase from Marinomonas sp. BSi20584 was successfully expressed in E. coli and showed stable soluble form. The purified enzyme had a specific activity of 96.827 U mg(-1) at 30 degrees C using ONPG as a substrate. It had an optimum pH of 7.0 and an optimum temperature of 50 degrees C. The enzyme's activity was enhanced by some divalent cations and inhibited by EDTA. Despite being derived from a cold-adapted strain, the enzyme exhibited considerable stability against various physical and chemical elements. It also showed activity towards rare substrates. The enzyme is a promising candidate for the industrial application of beta-galactosidase.
Review
Biotechnology & Applied Microbiology
Yanbo Hu, Huili Hong, Jianing Zhou, Yangyang Cui, Baochun Zhang, Jun Zhao
Summary: Bifidobacterium is an important probiotic in the intestinal gut flora, and its glycoside hydrolase plays a crucial role in its physiological activity. This review summarizes the preparation methods, enzymatic properties, and functions of glycoside hydrolase extracted from Bifidobacterium, providing a basis for the development of Bifidobacterium as a probiotic element.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Huitang Qi, Xi Jiang, Yi Ding, Tian Liu, Qing Yang
Summary: Kasugamycin, an aminoglycoside antibiotic, has been found to be a competitive inhibitor of glycoside hydrolase family 18 (GH18) chitinases in various organisms. It was discovered that the electrostatic interaction between kasugamycin and a conserved aspartate in GH18 chitinase is vital for its inhibitory activity. This research not only identifies new molecular targets of kasugamycin but also expands the understanding of GH inhibitor design by utilizing a scaffold unrelated to the substrate.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)