Article
Microbiology
Erica C. Keffeler, Vijayalakshmi S. Iyer, Srivatsan Parthasarathy, Matthew M. Ramsey, Matthew J. Gorman, Theresa L. Barke, Sriram Varahan, Sally Olson, Michael S. Gilmore, Zakria H. Abdullahi, Emmaleigh N. Hancock, Lynn E. Hancock
Summary: The alternative sigma factor s54 and its enhancer binding protein MptR in Enterococcus faecalis are essential for mannose utilization and glucose uptake, contributing to global transcriptional changes. Disruption of these factors significantly attenuates bacterial infection outcomes, highlighting the importance of central carbon metabolism in the pathogenesis of Enterococcus faecalis. Targeting metabolic pathways could lead to new treatments against multidrug-resistant enterococcal infections.
Article
Biotechnology & Applied Microbiology
Daisuke Fukuda, Yuji Aso, Cirilo Nolasco-Hipolito
Summary: Enterococcus faecalis strain DB-5 is a newly isolated lactic acid bacterium that can produce organic acid from various carbohydrate sources. It has excellent potential for lactic acid fermentation (LAF) and can efficiently convert sucrose to lactic acid at high temperatures. These findings provide valuable information for understanding the functional properties of future high-temperature LAFs from biomass resources.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2023)
Article
Microbiology
Peter Cathro, Peter McCarthy, Peter Hoffmann, Stephen Kidd, Peter Zilm
Summary: Enterococcus faecalis cell membrane responds to extreme alkaline conditions by shifting carbohydrate metabolism and forming a protective capsule.
FEMS MICROBIOLOGY LETTERS
(2022)
Article
Medicine, Research & Experimental
Li Zhang, Jing Liu, Mingxia Deng, Xiangliu Chen, Lushun Jiang, Jiajie Zhang, Lisheng Tao, Wei Yu, Yunqing Qiu
Summary: This study explores the effect of specific metabolites of Efa on CRC and identifies biliverdin (BV) as the key metabolite that promotes tumor growth and angiogenesis in CRC by activating the PI3K/AKT/mTOR signaling pathway. The findings were confirmed in both in vitro and in vivo experiments.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Microbiology
Jiazhen Liu, Yanpeng Zhu, Yang Li, Yuwen Lu, Kun Xiong, Qiu Zhong, Jing Wang
Summary: In this study, a bacteriophage EFap02 with the potential for treating drug-resistant Enterococcus faecalis infection was isolated. The phage proved to be tolerant to harsh environments and high temperature. However, phage-resistant mutants were found to have impaired biofilm formation.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
Mahdi Asghari Ozma, Ehsaneh Khodadadi, Mohammad Ahangarzadeh Rezaee, Fadhil S. Kamounah, Mohammad Asgharzadeh, Khodaverdi Ganbarov, Mohammad Aghazadeh, Mehdi Yousefi, Tahereh Pirzadeh, Hossein Samadi Kafil
Summary: The study found that gentamicin significantly increased biofilm formation in some E. faecalis isolates and led to changes in the expression levels of specific proteins. This may result in poor treatment outcomes and increased pathogenicity of the bacteria.
MICROBIAL PATHOGENESIS
(2021)
Article
Microbiology
Seyed Babak Loghmani, Eric Zitzow, Gene Ching Chiek Koh, Andreas Ulmer, Nadine Veith, Ruth Grosseholz, Madlen Rossnagel, Maren Loesch, Ruedi Aebersold, Bernd Kreikemeyer, Tomas Fiedler, Ursula Kummer
Summary: Lactic acid bacteria (LAB) play a significant role in biotechnology and human health, but many LAB genera have developed multidrug resistance, causing problems in controlling hospital germs. This study investigated the metabolic adaptations of Enterococcus faecalis, a common LAB causing human infections, under different environmental pH conditions. The study found that glutamine auxotrophy leads to increased energy demand.
MICROBIOLOGY SPECTRUM
(2022)
Article
Agronomy
Fenqi Chen, Xiangzhuo Ji, Zelong Zhuang, Yunling Peng
Summary: This study investigated the response of two maize inbred lines to salt stress at the transcriptional and proteomic levels. The results showed major differences in the regulation of gene expression and protein synthesis under salt stress. Furthermore, the mechanisms involved in the tolerance of maize to salt stress were identified, including enhanced lipid metabolism, lignin biosynthesis, activation of the abscisic acid signaling pathway, maintenance of dynamic energy balance, and enhanced reactive oxygen species clearance and protection.
Article
Biotechnology & Applied Microbiology
Marco Felipe Salas-Orozco, Nereyda Nino-Martinez, Gabriel-Alejandro Martinez-Castanon, Fernando Torres Mendez, Gabriela Margarita Montero Moran, Aranza Eliana Bendana-Pineiro, Facundo Ruiz, Horacio Bach
Summary: The study found that E. faecalis is capable of developing resistance to silver NPs after exposure to sublethal concentrations. To prevent the dissemination of resistant strains, future use of AgNPs should be combined with other NPs prepared with different metals.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Kentaro Hiraka, Hiromi Yoshida, Wakako Tsugawa, Ryutaro Asano, Jeffrey T. La Belle, Kazunori Ikebukuro, Koji Sode
Summary: This study investigates the structure and enzymatic properties of wild-type and mutant l-Lactate oxidase (LOx) from Enterococcus hirae. The structures reveal the product-inhibition mode of LOx and the potential role of Met207 as an oxygen gatekeeper residue. Three clades of LOx are proposed based on the difference of the Met207 position and their oxygen migration pathway.
Article
Agriculture, Multidisciplinary
Qi Zheng, Rong-cui Hu, Cui-yun Zhu, Jing Jing, Meng-yu Lou, Si-huan Zhang, Shuang Li, Hong-guo Cao, Xiao-rong Zhang, Ying-hui Ling
Summary: This study characterized the changes in proteomic and transcriptomic expression profiles during the transition from skeletal muscle satellite cells (SSCs) to myotube formation. Potential molecular mechanisms and signaling pathways involved in this transition were identified, and the roles of specific proteins such as SLMAP, STOM, SVIL, AQP1, and SERPINE1 were highlighted. These findings enhance our understanding of myotube formation during early development and repair.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2023)
Article
Microbiology
Mengfan Xu, Shanhu Hu, Yiwen Wang, Tao Wang, Piotr Dziugan, Bolin Zhang, Hongfei Zhao
Summary: The study found that Lactobacillus helveticus utilizes protein by extracellular hydrolysis and upregulation of various factors to prepare for proteolysis. Changes in key factors associated with glycolysis, trehalose phosphotransferase system (PTS), and aerobic respiration were observed during casein utilization in these bacteria.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Plant Sciences
Zhengfu Fang, Chunling Liu, Tiantian Ye, Chao Huang, Zhewei Zhang, Yanping Wang, Zhulong Chan
Summary: In this study, the transcriptomic and proteomic changes of bermudagrass after drought, salt, and submergence treatments were compared. The results showed that a significant number of genes were commonly affected by these stress treatments. Various biological processes and pathways related to stress response and metabolism were enriched. The ABA pathway was activated, while the IAA pathway was repressed. The overexpression of the ABA-responsive transcription factor CdABF2 improved the ABA sensitivity and stress tolerance in Arabidopsis.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2022)
Article
Plant Sciences
Wenji Li, Huigen Li, Lisha Shi, Ping Shen, Yurong Li
Summary: The difference in leaf color among the three cultivars of A. bettzickiana is due to different chloroplast morphology and chlorophyll-to-anthocyanin ratios. The study provides a good explanation for the coloration of different cultivars of A. bettzickiana and an important reference for better explaining the color formation mechanisms of plant leaves.
Article
Biotechnology & Applied Microbiology
Limei Chen, Xiumin Liu, Chaofeng Li, Hanchen Li, Wuxi Chen, Demao Li
Summary: This study investigated the mechanism of high DHA production in Schizochytrium limacinum LD11 and found that certain genes were upregulated, leading to increased synthesis of acetyl-CoA and triacylglycerol. The study also revealed that genes involved in β-oxidation were upregulated and downregulated, resulting in a significant increase in DHA content.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2022)