Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 77, Issue 6, Pages 2192-2195Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.01561-10
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Funding
- International Foundation for Ethical Research
- National Science Foundation [0622743]
- National Institute for Allergy and Infectious Diseases [R01AI068854]
- U.S. National Institute of Allergy and Infectious Diseases [N01-A1-30026]
- Dongchuan Qiu of the Institute of Parasitic Diseases
- Sichuan Center for Disease Control and Prevention, Chengdu, People's Republic of China
- Directorate For Geosciences [0622743] Funding Source: National Science Foundation
- Division Of Ocean Sciences [0622743] Funding Source: National Science Foundation
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A species-specific quantitative PCR (qPCR) assay was combined with two novel water-sampling methods and compared with the mouse bioassay for the quantitative detection of S. japonicum in surface waters. The novel methods were capable of capturing cercariae and, with subsequent analysis through qPCR, detecting the presence of a minimum of 1 cercaria.
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