Article
Immunology
Carina M. Hall, Nathan E. Stone, Madison Martz, Shelby M. Hutton, Ella Santana-Propper, Lora Versluis, Kieston Guidry, Marielisa Ortiz, Joseph D. Busch, Trevor Maness, Jonathan Stewart, Tom Sidwa, Jay E. Gee, Mindy G. Elrod, Julia K. Petras, Maureen C. Ty, Christopher Gulvik, Zachary P. Weiner, Johanna S. Salzer, Alex R. Hoffmaster, Sarai Rivera-Garcia, Paul Keim, Amanda Kieffer, Jason W. Sahl, Fred Soltero, David M. Wagner
Summary: Burkholderia thailandensis, a bacterium closely related to the pathogen B. pseudomallei, was found in water in Texas and Puerto Rico, as well as soil in Mississippi in the United States. This discovery highlights a potential public health risk, although human infections with B. thailandensis are rare.
EMERGING INFECTIOUS DISEASES
(2023)
Article
Microbiology
Sabine Lichtenegger, Trung T. Trinh, Karoline Assig, Karola Prior, Dag Harmsen, Julian Pesl, Andrea Zauner, Michaela Lipp, Tram A. Que, Beatrice Mutsam, Barbara Kleinhappl, Ivo Steinmetz, Gabriel E. Wagner
Summary: In this study, a WGS-based core genome multilocus sequence typing (cgMLST) scheme applicable in routine diagnostic settings was established and validated. The scheme was validated with 320 WGS data sets, showing >95% good cgMLST target genes in 98.4% of the genomes. Comparison with existing typing methods revealed very good concordance.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Microbiology
Carina M. M. Hall, Nawarat Somprasong, Johannah P. P. Hagen, Roxanne Nottingham, Jason W. W. Sahl, Jessica R. R. Webb, Mark Mayo, Bart J. J. Currie, Yuwana Podin, David M. M. Wagner, Paul Keim, Herbert P. P. Schweizer
Summary: Cefiderocol is a siderophore cephalosporin designed for treating infections caused by beta-lactam and multidrug-resistant Gram-negative bacteria. Resistance in clinical B. pseudomallei isolates from Australia is caused by an uncharacterized mechanism. The PiuA outer membrane receptor plays a major role in cefiderocol nonsusceptibility in isolates from Malaysia.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Immunology
Lisa J. Speiser, Sabirah Kasule, Carina M. Hall, Jason W. Sahl, David M. Wagner, Chris Saling, Amy Kole, Andrew J. Meltzer, Victor Davila, Robert Orenstein, Thomas Grys, Erin Graf
Summary: Melioidosis, caused by Burkholderia pseudomallei, is a highly lethal infection with a particularly high fatality rate if left untreated or undiagnosed. It is endemic to Asia, Australia, South America, and the Caribbean, but the number of cases reported in the United States is increasing. This study highlights the rare occurrence of melioidosis presenting as a mycotic aneurysm in the United States and emphasizes the need for diagnostic awareness and epidemiological concerns.
OPEN FORUM INFECTIOUS DISEASES
(2022)
Article
Multidisciplinary Sciences
Khemngeun Pongmala, Alain Pierret, Priscia Oliva, Anne Pando, Viengmon Davong, Sayaphet Rattanavong, Norbert Silvera, Manophab Luangraj, Laurie Boithias, Khampaseuth Xayyathip, Ludovic Menjot, Melina Macouin, Emma Rochelle-Newall, Henri Robain, Amphone Vongvixay, Andrew J. H. Simpson, David A. B. Dance, Olivier Ribolzi
Summary: This paper provides novel information about the distribution of Burkholderia pseudomallei in soil and its potential association with soil biogeochemical heterogeneity and vertical distribution. The study highlights the complexity of soil and emphasizes the importance of collecting samples below the water table in environmental sampling for B. pseudomallei.
SCIENTIFIC REPORTS
(2022)
Letter
Immunology
Courtney M. Dewart, Francisco A. Almeida, Christine Koval, Scott Nowicki, Jay E. Gee, Mindy Glass Elrod, Christopher A. Gulvik, Johanna S. Salzer, Sietske de Fijter, Lindy Liu
Summary: Phylogenetic analysis of a clinical isolate associated with subclinical Burkholderia pseudomallei infection revealed probable exposure in the British Virgin Islands, where reported infections are limited. Clinicians should consider this geographic distribution when evaluating possible infection among persons with compatible travel history.
EMERGING INFECTIOUS DISEASES
(2021)
Article
Chemistry, Multidisciplinary
Michaela Prothiwa, Verena Filz, Sebastian Oehler, Thomas Boettcher
Summary: A novel substrate-based chemical probe was developed against the central quinolone biosynthesis enzyme HmqD in Burkholderia species, leading to the discovery of the first known HmqD inhibitors with single-digit micromolar efficacy in blocking quinolone production in Burkholderia cultures.
Review
Immunology
Vanitha Mariappan, Kumutha Malar Vellasamy, Muttiah Barathan, A. S. Smiline Girija, Esaki M. Shankar, Jamuna Vadivelu
Summary: B. pseudomallei infection causes melioidosis with varying severity, diverse pathological manifestations, complex mechanisms of latency and relapse, and the crucial role of immune response in eradicating the pathogen. However, the question of why some individuals are asymptomatic while others are life-threatening remains unanswered.
FRONTIERS IN IMMUNOLOGY
(2021)
Letter
Immunology
Himali S. Jayasinghearachchi, Vaithehi R. Francis, Harindra D. Sathkumara, Shivankari Krishnananthasivam, Jayanthi Masakorala, Thilini Muthugama, Aruna D. De Silva, Enoka M. Corea
Summary: A cluster of melioidosis cases occurred in eastern Sri Lanka, with four infections caused by Burkholderia pseudomallei isolates of sequence type 594. Whole-genome analysis revealed genetic diversity among the isolates and showed that the cluster was nonclonal.
EMERGING INFECTIOUS DISEASES
(2021)
Article
Biochemistry & Molecular Biology
Shengyuan Huo, Xiao Li, Shiwei Wang, Pan Wu, Dongqi Nan, Chenglong Rao, Qian Li, Xuhu Mao, Jingmin Yan
Summary: This study investigated the structural elucidation of type B O antigen from a clinical B. pseudomallei strain and the effects of different types of LPS on macrophage differentiation. The O antigen was found to consist of repeating units with specific substitutions. Both type A and type B O antigens could polarize macrophages towards the M1 phenotype. Characterizing different types of O antigen structural motifs is essential for understanding the persistence/survival mechanisms and inflammatory potential of B. pseudomallei.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Microbiology
Kasturi Selvam, Muhammad Fazli Khalid, Khairul Mohd Fadzli Mustaffa, Azian Harun, Ismail Aziah
Summary: Melioidosis is a severe disease caused by Burkholderia pseudomallei, mainly affecting immunocompromised populations such as patients with type 2 diabetes mellitus. Laboratory diagnosis is challenging due to non-specific clinical manifestations and the imperfect sensitivity of culture methods.
Article
Biochemistry & Molecular Biology
Claire Chewapreecha, Johan Pensar, Supaksorn Chattagul, Maiju Pesonen, Apiwat Sangphukieo, Phumrapee Boonklang, Chotima Potisap, Sirikamon Koosakulnirand, Edward J. Feil, Susanna Dunachie, Narisara Chantratita, Direk Limmathurotsakul, Sharon J. Peacock, Nick P. J. Day, Julian Parkhill, Nicholas R. Thomson, Rasana W. Sermswan, Jukka Corander
Summary: The research found that specific genes and non-coding RNA of the soil bacterium Burkholderia pseudomallei are under the influence of co-selection during evolution, with these genes showing significant expression correlation under physical stress conditions, and a putative adhesin playing an important role under nutrient deprivation.
MOLECULAR BIOLOGY AND EVOLUTION
(2022)
Article
Immunology
Sarah M. Baker, Erik W. Settles, Christopher Davitt, Patrick Gellings, Nicole Kikendall, Joseph Hoffmann, Yihui Wang, Jacob Bitoun, Kasi-Russell Lodrigue, Jason W. Sahl, Paul Keim, Chad Roy, James McLachlan, Lisa A. Morici
Summary: Burkholderia pseudomallei OMVs produced under macrophage-mimicking growth conditions (M9 OMVs) contain proteins associated with intracellular survival, provide significant protection against pulmonary infection in mice, possess adjuvanticity, and may serve as a promising vaccine against melioidosis.
Article
Microbiology
Ahmad-Kamal Ghazali, Mohd Firdaus-Raih, Asqwin Uthaya Kumar, Wei-Kang Lee, Chee-Choong Hoh, Sheila Nathan
Summary: This study investigated the transcriptome-level adaptations of B. pseudomallei when growing in soil and during infection. The results showed that the bacterium induced the expression of metabolism and transporter proteins in soil, while virulence factors, metabolism, and bacterial defense mechanisms were upregulated during infection. Additionally, the capsular polysaccharide and quorum-sensing pathways were inhibited during infection. These findings provide crucial insights into the adaptation mechanisms of B. pseudomallei in soil and during infection.
MICROBIOLOGY SPECTRUM
(2023)
Review
Microbiology
Ella M. Meumann, Direk Limmathurotsakul, Susanna J. Dunachie, Willem J. Wiersinga, Bart J. Currie
Summary: This review explores the global burden, epidemiology, and pathophysiology of Burkholderia pseudomallei, the causative agent of melioidosis, as well as current diagnostics, treatment recommendations, and preventive measures. Melioidosis is widely distributed in tropical and subtropical regions and has been proposed as a neglected tropical disease by the World Health Organization. Climate change and environmental disturbance are expected to increase the number of cases.
NATURE REVIEWS MICROBIOLOGY
(2023)
Article
Infectious Diseases
Vanina Guernier, Tanya Diefenbach-Elstob, Daniel Pelowa, Sandra Pollard, Graham Burgess, Emma S. McBryde, Jeffrey Warner
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2018)
Article
Public, Environmental & Occupational Health
Tanya Diefenbach-Elstob, Patricia Graves, Robert Dowi, Bisato Gula, David Plummer, Emma McBryde, Daniel Pelowa, Peter Siba, William Pomat, Jeffrey Warner
TROPICAL MEDICINE & INTERNATIONAL HEALTH
(2018)
Article
Ornithology
Hannah G. Smith, Rohan H. Clarke, Jo-Ann Larkins, David C. Bean, Andrew R. Greenhill
EMU-AUSTRAL ORNITHOLOGY
(2019)
Article
Multidisciplinary Sciences
Ahmed Al-Jumaili, Peter Mulvey, Avishek Kumar, Karthika Prasad, Kateryna Bazaka, Jeffrey Warner, Mohan V. Jacob
SCIENTIFIC REPORTS
(2019)
Article
Engineering, Chemical
Avishek Kumar, Ahmed Al-Jumaili, Karthika Prasad, Kateryna Bazaka, Peter Mulvey, Jeffrey Warner, Mohan Jacob
PLASMA CHEMISTRY AND PLASMA PROCESSING
(2020)
Article
Multidisciplinary Sciences
Vanina Guernier-Cambert, Tanya Diefenbach-Elstob, Bernice J. Klotoe, Graham Burgess, Daniel Pelowa, Robert Dowi, Bisato Gula, Emma S. McBryde, Guislaine Refregier, Catherine Rush, Christophe Sola, Jeffrey Warner
SCIENTIFIC REPORTS
(2019)
Article
Biochemistry & Molecular Biology
Akhikun Nahar, Anthony L. Baker, David S. Nichols, John P. Bowman, Margaret L. Britz
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biotechnology & Applied Microbiology
Hannah G. Smith, David C. Bean, Jane Hawkey, Rohan H. Clarke, Richard Loyn, Jo-Ann Larkins, Chris Hassell, Mary Valcanis, William Pitchers, Andrew R. Greenhill
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2020)
Article
Public, Environmental & Occupational Health
Hannah G. Smith, David C. Bean, Rohan H. Clarke, Richard Loyn, Jo-Ann Larkins, Chris Hassell, Andrew R. Greenhill
Summary: Antibiotic resistance poses a continuous threat to human and animal health, with migratory birds potentially serving as vectors for the spread of novel pathogens and antibiotic resistance genes. This study in Australian shorebirds and terns found a high prevalence of antibiotic resistance in commonly isolated bacteria, suggesting that wild bird populations may serve as potential reservoirs and vectors for antibiotic resistance bacteria.
ZOONOSES AND PUBLIC HEALTH
(2022)
Article
Biochemistry & Molecular Biology
Yomani D. Sarathkumara, Daniel J. Browne, Ashton M. Kelly, David J. Pattinson, Catherine M. Rush, Jeffrey Warner, Carla Proietti, Denise L. Doolan
Summary: This study compared the performance of two widely used whole-blood RNA collection systems, PAXgene and Tempus, in laboratory and suboptimal tropical field conditions. Tempus tubes maintained higher RNA quantity and integrity compared to PAXgene under suboptimal conditions and also showed better stability of mRNA transcripts for reference genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Hannah G. P. Smith, David C. Bean, William Pitchers, Mary Valcanis, Rohan H. Clarke, Richard Loyn, Chris J. Hassell, Andrew R. Greenhill
Summary: The study analyzed the draft genome sequences of Citrobacter isolates obtained from wild Australian shorebirds, revealing their presence in the environment. This helps understand the transmission and impact of Citrobacter in wild animals. The research provides insights into the spread of antimicrobial resistance genes in wildlife.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2021)
Article
Microbiology
Md Abdullah Al Mamun, Mark Sandeman, Phil Rayment, Phillip Brook-Carter, Emily Scholes, Naga Kasinadhuni, David Piedrafita, Andrew R. Greenhill
Article
Agronomy
Muhammad Zahid Mumtaz, Karen M. Barry, Anthony L. Baker, David S. Nichols, Maqshoof Ahmad, Zahir Ahmad Zahir, Margaret L. Britz
Article
Infectious Diseases
Tanya Diefenbach-Elstob, Vanina Guernier, Graham Burgess, Daniel Pelowa, Robert Dowi, Bisato Gula, Munish Puri, William Pomat, Emma McBryde, David Plummer, Catherine Rush, Jeffrey Warner
TROPICAL MEDICINE AND INFECTIOUS DISEASE
(2019)
Article
Infectious Diseases
Jeffrey M. Warner, Bart J. Currie
TROPICAL MEDICINE AND INFECTIOUS DISEASE
(2018)