Article
Microbiology
Roua Almatrafi, Saeed Banawas, Mahfuzur R. Sarker
Summary: Spore germination is crucial in the development of Clostridium perfringens-associated food poisoning. A previous study found that dipicolinic acid (DPA) chelated with calcium (Ca-DPA) stimulates spore germination. This study aimed to explore the roles of Ca2+, Mn2+, and Mg2+ in spore germination. The results showed that Ca-DPA, but not DPA alone, induced C. perfringens spore germination. Additionally, spore-specific divalent cations Mn2+ and Mg2+, but not Zn2+, were found to induce spore germination.
Article
Biotechnology & Applied Microbiology
Juan Wen, Jan P. P. M. Smelt, Norbert O. E. Vischer, Arend L. de Vos, Peter Setlow, Stanley Brul
Summary: The heat activation and heat damage profile of Bacillus subtilis spores were determined in this study. Heat activation at 40 to 70 degrees C promoted spore germination, while heat inactivation and damage to germination-related molecules occurred at 70 degrees C.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Review
Food Science & Technology
Fengzhi Lyu, Tianyu Zhang, Meng Gui, Yongtao Wang, Liang Zhao, Xiaomeng Wu, Lei Rao, Xiaojun Liao
Summary: Bacterial spores, highly resilient and universally present, can cause food spoilage or foodborne illness once revived. Traditional thermal processing can kill spores but adversely affects food quality attributes. The germination-inactivation strategy, which mildly kills spores, faces challenges due to the heterogeneous germination behavior. Understanding germination pathways and mechanisms can facilitate the development of effective strategies. This review discusses the mechanisms of spore germination and the potential application of high hydrostatic pressure (HHP) in mild food sterilization.
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY
(2023)
Article
Microbiology
Marketa Husakova, Michaela Plechata, Barbora Branska, Petra Patakova
Summary: The study found that the addition of nitrite alone had no effect on spore germination, but when combined with pickling salt, it effectively inhibited the germination of Clostridium spores. Red yeast rice (RYR) extract was able to completely suppress the germination of Clostridium spores, while having a lesser effect on Bacillus spores.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Benjamin Yu, Julia Kanaan, Hannah Shames, James Wicander, Makunda Aryal, Yunfeng Li, George Korza, Stanley Brul, Gertjan Kramer, Yong-qing Li, Frank C. Nichols, Bing Hao, Peter Setlow
Summary: 2Duf is a protein located in the inner membrane of spores in Bacillus species. The absence of YetF or YdfS leads to decreased resistance to wet heat and damage to spore core components. YetF can modify the inner membrane structure to increase spore resistance to wet heat.
FRONTIERS IN MICROBIOLOGY
(2023)
