Review
Microbiology
Xin Yin, Ying-Zhou Chen, Qi-Qing Ye, Li-Juan Liao, Zhuo-Rui Cai, Min Lin, Jia-Na Li, Geng-Biao Zhang, Xiao-Li Peng, Wen-Fang Shi, Xu-Guang Guo
Summary: Legionellosis remains a public health problem, with PCR showing high efficiency and accuracy in detecting L. pneumophila. Analysis of 18 four-fold tables from 16 studies revealed a sensitivity of 94% for PCR in diagnosing L. pneumophila.
ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS
(2022)
Article
Biochemistry & Molecular Biology
Atosa Ghorbani, Mohammad Hashemzadeh, Mansour Amin, Mojtaba Moosavian, Rohangiz Nashibi, Zahra Mehraban
Summary: This study investigated the occurrence of Legionella species in respiratory samples of pneumonia patients from Ahvaz, Iran using culture and real-time PCR. Real-time PCR was more effective in detecting Legionella species, with L. pneumophila identified as the most prevalent species in the region. Periodic monitoring programs are recommended to prevent epidemics caused by this bacterium.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Environmental Sciences
Yoontaek Oh, Jatuwat Sangsanont, Hyoungmin Woo, Laura A. Boczek, Karl G. Linden, Hodon Ryu
Summary: In the United States, Legionnaires' disease infections caused by Legionella have increased drastically from 2000 to 2018. The presence of Legionella in biofilms or within amoeba in plumbing systems makes it more resistant to disinfectants, posing challenges for treatment. UV-LEDs are a promising water disinfection technology with advantages over conventional UV lamps. This study evaluated the effects of UV-LEDs and UV lamps of different wavelengths on the properties and inactivation of three strains of L. pneumophila serogroup 1. Results showed that UV-LEDs at 255 nm and 265 nm were more effective in inactivating all strains compared to UV-LEDs at 285 nm and a low-pressure UV lamp at 254 nm. Different strains within the same serogroup exhibited varying sensitivities to UV radiation.
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)
Article
Engineering, Chemical
Masaki Okunaga, Kenta Kushiro, Ryohei Horie, Akihiro Kondo, Takashi Abe
Summary: In Japan, bathwaters in public facilities are a potential source of legionellosis. However, the current culture method for detecting Legionella spp. is time-consuming and has limited accuracy. This study investigated the microbiota of bathwater samples and identified several microbial species, such as Methyloversatilis, Cupriavidus, Phenylobacterium, Vermamoeba, and Aspidisca, that were highly correlated with Legionella spp. These findings contribute to the development of strategies against legionellosis.
Article
Chemistry, Analytical
M. Amirul Islam, Walid M. M. Hassen, Ishika Ishika, Azam F. F. Tayabali, Jan J. J. Dubowski
Summary: This study demonstrates the enhanced detection of Legionella pneumophila using a modified digital photocorrosion biosensor functionalized with cysteine-modified warnericin antimicrobial peptides and anti-L. pneumophila polyclonal antibodies. The combination of peptides and antibodies allows for highly specific and sensitive detection of L. pneumophila.
Article
Immunology
Xiaolin Jiang, Yong Jiang, Feng Ye
Summary: This study used real-time quantitative PCR to detect Mucorales and Aspergillus in formalin-fixed, paraffin-embedded samples. The combination of Mucorales RQ-PCR and Aspergillus RQ-PCR was recommended as a screening tool for detecting suspected mucormycosis and/or aspergillosis samples.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Environmental Sciences
Osvalda De Giglio, Marilena D'Ambrosio, Valentina Spagnuolo, Giusy Diella, Fabrizio Fasano, Carla Maria Leone, Marco Lopuzzo, Valeria Trallo, Carla Calia, Marta Oliva, Carlo Pazzani, Lucilla Iacumin, Sofia Barigelli, Maya Petricciuolo, Ermanno Federici, Francesco Paolo Lisena, Anna Maria Minicucci, Maria Teresa Montagna
Summary: Understanding the distribution of different Legionella species in water networks is important for preventing outbreaks. Various molecular techniques, including culture investigations, serological tests, MALDI-TOF, and mip-gene sequencing, were used to identify Legionella non pneumophila (L-np) strains isolated during environmental surveillance. Out of 346 water samples collected from a hospital in Italy, 24.9% were positive for Legionella, with Legionella pneumophila (Lpn) 1 being the most prevalent. Strains initially identified as L. bozemanii were reclassified as L. anisa using MALDI-TOF and mip-gene sequencing.
ENVIRONMENTAL MONITORING AND ASSESSMENT
(2023)
Article
Biotechnology & Applied Microbiology
D. Eble, V Gehrig, P. Schubert-Ullrich, R. Koeppel, H. P. Fuechslin
Summary: This study proposes the use of multiplex PCR to confirm Legionella colonies, allowing for faster detection of positive colonies with higher specificity. Analysis from two laboratories showed that the PCR technique has higher specificity.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Biology
Michael J. Sheedlo, Clarissa L. Durie, Jeong Min Chung, Louise Chang, Jacquelyn Roberts, Michele Swanson, Dana Borden Lacy, Melanie D. Ohi
Summary: This passage discusses the relationship between Legionella pneumophila and Legionnaires' disease, as well as the structure of the Dot/Icm T4SS core complex determined by single particle cryo-EM. It identifies new proteins contributing to the core T4SS structure and defines two areas of symmetry mismatch.
Article
Immunology
Milica Jovanovic, Nikola Mitrovic, Letitia Beraud, Nikola Trboljevac, Branko Milosevic, Aleksandra Radovanovic Spurnic, Snezana Jovanovic, Dragana Maric
Summary: Legionella pneumophila, a rarely diagnosed microorganism in Serbia, was successfully diagnosed and treated in a patient during the COVID-19 pandemic using multiplex PCR test, with serologic assays providing further confirmation of the diagnosis.
EUROPEAN JOURNAL OF INFLAMMATION
(2022)
Article
Public, Environmental & Occupational Health
M. Sauget, M. Richard, S. Chassagne, D. Hocquet, X. Bertrand, A. Jeanvoine
Summary: This study evaluated the performance of a qPCR method for the rapid monitoring of Legionella pneumophila (Lp) in hospital hot water networks (HWNs). The results showed that qPCR could be implemented as a complement to culture in the monitoring strategy to speed up the implementation of corrective measures.
JOURNAL OF HOSPITAL INFECTION
(2023)
Article
Food Science & Technology
Qinghua Ye, Yuting Shang, Moutong Chen, Rui Pang, Fan Li, Chufang Wang, Xinran Xiang, Baoqing Zhou, Shuhong Zhang, Jumei Zhang, Shi Wu, Liang Xue, Yu Ding, Qingping Wu
Summary: This study used whole-genome analysis to identify new serovar-specific genes for the most common serogroup B Salmonella serovars in China, and designed specific PCR primer sets for validation. PCR successfully detected all target Salmonella serovars in different types of food samples, with high analytical sensitivity. The findings highlight the value of whole-genome analysis in identifying new serovar-specific targets for developing rapid and accurate molecular detection methods.
Article
Biotechnology & Applied Microbiology
Juan Fernandez-Carrillo, Javier del Olmo-Monge, Ricela E. Sellek, Maria Victoria Ortega-Garcia, Juan Carlos Cabria-Ramos, Olga Bassy
Summary: This paper presents a specific real-time polymerase chain reaction (PCR) assay for the detection of Coxiella burnetii, which is highly infectious and considered a biological threat. The assay is based on amplification of a specific gene fragment and has high sensitivity, reproducibility, and specificity. It can differentiate strains based on a point mutation and has been successfully applied to environmental soil samples.
LETTERS IN APPLIED MICROBIOLOGY
(2023)
Article
Microbiology
Alicia Y. W. Wong, Alexander T. A. Johnsson, Aina Iversen, Simon Athlin, Volkan Ozenci
Summary: The study evaluated the performance of four different urinary antigen tests for the detection of Legionella urinary antigen. While these tests performed well in detecting samples from L. pneumophila serogroup 1-positive patients, they showed poor performance in detecting samples from other Legionella species or serogroups. The automatic and visual interpretation of result bands showed high concordance.
Article
Environmental Sciences
F. Mendoza-Cano, T. Encinas-Garcia, A. Muhlia-Almazan, M. Porchas-Cornejo, E. de la Re-Vega, A. Sanchez-Paza
Summary: Earth is home to various cellular forms that have adapted through natural selection. However, viruses outnumber and outdiversify cellular life. Among them, phages are prevalent and play significant roles in ecological processes. Pelagiphages, the most abundant viruses in the oceans, are understudied in their ecological contribution to Pelagibacterales populations. Accurate quantification of pelagiphages is crucial due to their abundance and claimed influence in element cycling. This study developed and validated a sensitive and specific qPCR platform for pelagiphages, allowing their detection and quantification in the Gulf of California for the first time.
MARINE ENVIRONMENTAL RESEARCH
(2023)
Review
Microbiology
Jessica E. Martyn, Laura Gomez-Valero, Carmen Buchrieser
Summary: Intracellular pathogens have cellular biology tools to influence their eukaryotic host and use protein secretion systems and molecular effectors to manipulate host cells. These effectors function in a eukaryotic context and mimic or inhibit eukaryotic proteins. Environmental intracellular bacteria have a higher number of eukaryotic-like domains and proteins compared to bacteria specialized to an isolated niche.
FEMS MICROBIOLOGY REVIEWS
(2022)
Article
Multidisciplinary Sciences
Tobias Sahr, Pedro Escoll, Christophe Rusniok, Sheryl Bui, Gerard Pehau-Arnaudet, Gregory Lavieu, Carmen Buchrieser
Summary: This study reveals that Legionella pneumophila mimics eukaryotic microRNA to modulate the host response to infection. This miRNA-like regulation is a feature of host-pathogen communication and may represent a general mechanism used by bacteria that interact with eukaryotic hosts.
NATURE COMMUNICATIONS
(2022)
Article
Microbiology
Hubert Hilbi, Carmen Buchrieser
Summary: Legionella pneumophila is a facultative intracellular pathogen that infects humans by parasitizing aquatic protozoa. It possesses a large repertoire of unique bacterial effectors that manipulate host cells, leading to severe diseases such as pneumonia.
Article
Health Care Sciences & Services
Diane Friedman, Lamiae Grimaldi, Alain Cariou, Philippe Aegerter, Stephane Gaudry, Abdel Ben Salah, Haikel Oueslati, Bruno Megarbane, Nicolas Meunier-Beillard, Jean-Pierre Quenot, Carole Schwebel, Laurent Jacob, Segloene Robin Lagandre, Pierre Kalfon, Romain Sonneville, Shidasp Siami, Aurelien Mazeraud, Tarek Sharshar
Summary: This study aims to compare a multidisciplinary follow-up with a standard follow-up for critically ill patients, and to evaluate the impact on quality of life (QoL) at 1 year. The study found that multidisciplinary follow-up may improve QoL by mitigating the postintensive care syndrome (PICS).
JMIR RESEARCH PROTOCOLS
(2022)
Article
Chemistry, Medicinal
Alexandre Bruneau, Emilie Gillon, Etienne Brachet, Mouad Alami, Christine Roques, Annabelle Varrot, Anne Imberty, Samir Messaoudi
Summary: In this study, beta-thiogalactoside mimetics bearing 1,1-diarylmethylene or benzophenone aglycons were synthesized and tested for their affinity towards LecA. The hit compound showed higher efficiency and additional contacts with the protein surface were confirmed by crystal structure. The highest affinity was observed for a divalent ligand containing two galactosides. The monovalent high affinity compound obtained through structure-activity relationship (SAR) displayed effective antibiofilm activity without bactericidal activity.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Sandrine Laurance, Mickael Marin, Yves Colin
Summary: Central retinal vein occlusion (CRVO) is a common retinal disorder leading to blindness, but the primary cause and treatment remain unidentified. This review aims to provide an overview of CRVO pathophysiology, specifically focusing on the role of red blood cells (RBCs). The review explores the functions of RBCs beyond oxygen transport and their potential involvement in CRVO occurrence.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Arthur Louche, Amandine Blanco, Thais Lourdes Santos Lacerda, Lison Cancade-Veyre, Claire Lionnet, Celia Berge, Monica Rolando, Frederique Lembo, Jean-Paul Borg, Carmen Buchrieser, Masami Nagahama, Francine C. A. Gerard, Jean-Pierre Gorvel, Virginie Gueguen-Chaignon, Laurent Terradot, Suzana P. P. Salcedo
Summary: The cell nucleus is a primary target for intracellular bacterial pathogens. Two Brucella abortus effectors, NyxA and NyxB, interfere with host protease SENP3, promoting intracellular replication of the pathogen.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Daniel Schator, Sonia Mondino, Jeremy Berthelet, Cristina Di Silvestre, Mathilde Ben Assaya, Christophe Rusniok, Fernando Rodrigues-Lima, Annemarie Wehenkel, Carmen Buchrieser, Monica Rolando
Summary: Legionella pneumophilia secretes more than 300 effectors, including RomA and LphD, via a type IV secretion system. RomA methylates histone H3 at K14 to counteract host immune responses, while LphD deacetylates H3K14 to work synergistically with RomA. The two effectors target host chromatin and bind the HBO1 histone acetyltransferase complex to regulate histone modifications.
NATURE COMMUNICATIONS
(2023)
Article
Microbiology
Flavia Viana, Laurent Boucontet, Valerio Laghi, Daniel Schator, Marine Ibranosyan, Sophie Jarraud, Emma Colucci-Guyon, Carmen Buchrieser
Summary: By developing a zebrafish model, we discovered the association between host immune response and Legionella pneumophila infection, with macrophages being the first line of defense and cooperating with neutrophils to clear the infection. Additionally, we found that immunocompromised zebrafish larvae are exceptionally sensitive to L. pneumophila. The zebrafish model can mimic the immune response to L. pneumophila infection in humans and help elucidate the mechanisms by which L. pneumophila crosses host cell membranes and obtains nutrients.
Correction
Health Care Sciences & Services
Diane Friedman, Lamiae Grimaldi, Alain Cariou, Philippe Aegerter, Stephane Gaudry, Abdel Ben Salah, Haikel Oueslati, Bruno Megarbane, Nicolas Meunier-Beillard, Jean-Pierre Quenot, Carole Schwebel, Laurent Jacob, Segloene Robin Lagandre, Pierre Kalfon, Romain Sonneville, Shidasp Siami, Aurelien Mazeraud, Tarek Sharshar
JMIR RESEARCH PROTOCOLS
(2023)
Article
Chemistry, Organic
Yurong Yuan, Clara Faure, Doriane Menigaux, Mathieu Berthelot, Philippe Belmont, Etienne Brachet
Summary: Visible-light catalysis is an effective method for constructing various types of compounds. Further advancements are needed, but a seamless reaction pathway has been presented for synthesizing compounds with 6-membered ring nitrogen-containing heterocycles.
JOURNAL OF ORGANIC CHEMISTRY
(2023)
Article
Cell Biology
Ana Elena Perez-Cobas, Christophe Ginevra, Christophe Rusniok, Sophie Jarraud, Carmen Buchrieser
Summary: This study examines the respiratory tract microbiome (RTM) of 38 patients with pneumonia caused by Legionella pneumophila. The composition of the RTM changes early in hospitalization, with a decrease in diversity and replacement of species. RTMs with higher bacterial and fungal loads show lower diversity and pathogen enrichment, suggesting that high biomass may be a biomarker for secondary and/or co-infections. The structure of the RTM is defined by a commensal cluster associated with a healthy RTM and a pathogen-enriched cluster, indicating that the balance between these clusters plays a critical role in patient recovery.
CELL REPORTS MEDICINE
(2023)
Article
Cell Biology
Clarisse Leseigneur, Carmen Buchrieser
Summary: The study of the virulence of Legionella pneumophila and its interactions with hosts has mostly been conducted using cellular models. However, these models fail to accurately represent the complexity of the environment encountered by the bacteria during infection in multicellular organisms. To better understand human infection, various animal models have been developed as alternatives to the traditional mouse model. This review provides an overview of these invertebrate and vertebrate models and their contributions, as well as their advantages and disadvantages.
EUROPEAN JOURNAL OF CELL BIOLOGY
(2023)
Article
Infectious Diseases
Victor Dechaene, Clementine Gallet, Sarah Soueges, Lannie Liu, Violaine Delabar, Leopold Adelaide, Sophie Jarraud, Olivier Dauwalder, Emmanuel Jouanneau, Marie Wan, Timothee Jacquesson, Jacques Guyotat, Anne Conrad, Claire Triffault-Fillit, Tristan Ferry, Florent Valour
Summary: This study aimed to describe the diagnosis, management, and outcome of bone flap-related osteomyelitis after cranioplasty. The study found that most patients presented wound abnormalities, and the main pathogens were Staphylococcus aureus, Cutibacterium acnes, gram-negative bacilli, and non-aureus staphylococci. Follow-up after surgery and antimicrobial therapy showed that some patients experienced treatment failure, which was associated with factors such as the cause of the craniectomy and the surgical approach.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2023)
