Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 76, Issue 13, Pages 4553-4556Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.00226-10
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Funding
- NIH [1R01 GM73821, 1S10RR02260601]
- M. J. Murdock Charitable Trust [2006-127JVZ022207]
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We designed a new genetic tool to detect plasmid transfer under anaerobic and aerobic conditions. The system is based on the T7 RNA polymerase gene and a T7 promoter-driven oxygen-independent green fluorescent protein, evoglow, alone or in combination with red fluorescent protein DsRed. Constructs are available as plasmids and mini-mariner transposons.
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