Article
Environmental Sciences
Marcel Pierre Simon, Dennis Knuth, Leonard Boehm, Katrin Wiltschka, Marlene Schatz, Rolf-Alexander Duering
Summary: The study presented a simple, cheap, and fast method for analyzing organochlorine pesticide residues in agricultural soils to assess the risks. The new method was found to be quicker than the QuEChERS method and requires less sample preparation volume.
JOURNAL OF SOILS AND SEDIMENTS
(2022)
Article
Chemistry, Analytical
Jiafu Li, Zengli Zhang, Md. Tareq Aziz, Caroline O. Granger, Zhimin Qiang, Susan D. Richardson, Huiyu Dong
Summary: Due to the high concentrations of disinfection byproducts (DBPs) in drinking water, compared to other emerging environmental contaminants, DBPs have become a global concern. We have developed a simple and sensitive method for simultaneously measuring 9 classes of DBPs. This method overcomes the weaknesses of other methods, particularly for HAAs/IAAs and mono-/di-HALs, and provides a useful tool for studying regulated and unregulated priority DBPs.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Meire Ribeiro da Silva, Izabela Zaborowska, Sara Carillo, Jonathan Bones
Summary: The development and optimization of cell culture media for biotech applications is crucial for ensuring cell growth and productivity. The microfluidic chip CE-MS method described in this study allows for rapid monitoring of amino acids in cell culture media, providing valuable insights into consumption rates and cell culture status. This approach offers a practical and efficient solution for analyzing complex cell culture media.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biochemical Research Methods
Miftakh Nur Rahman, Ajeng Diantini, Miswar Fattah, Melisa I. Barliana, Andi Wijaya
Summary: Short-chain fatty acids (SCFAs) are important gut microbiota metabolites associated with metabolic diseases, particularly central obesity. A highly sensitive, simple, and fast method for measuring SCFAs in serum was developed using gas chromatography-mass spectrometry. Results showed significantly higher levels of all SCFAs in centrally obese subjects compared to lean subjects, indicating potential features of circulating SCFAs in central obesity.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Gabriele Sass, Marie-Christine Groleau, Eric Deziel, David A. Stevens
Summary: This study presents a simple method for quantifying biosurfactants based on their ability to reduce surface tension of aqueous solutions on paper. The method is applicable to various types of surfactants and easily accessible in any laboratory.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Martina Zanchetta, Valentina Iacuzzi, Bianca Posocco, Giorgia Bortolin, Ariana Soledad Poetto, Marco Orleni, Giovanni Canil, Michela Guardascione, Luisa Foltran, Valentina Fanotto, Fabio Puglisi, Sara Gagno, Giuseppe Toffoli
Summary: A sensitive, rapid, and cost-effective LC-MS/MS method was developed and validated for the quantification of LENVA in human plasma in this study. The method met EMA and FDA guidelines with good linearity, precision, and accuracy, and was successfully applied to patient plasma samples.
Article
Chemistry, Analytical
Dingyan Fan, Yingzhou Zhang, Haiping Wu
Summary: A method for quantitative determination of vitamin B12 in infant food was developed using hydrophilic HPLC coupled with DAD, with enhanced sensitivity of the detector using a high-sensitivity LightPipe flow cell. The method showed a good linear analytical range and repeatability, with detailed optimization of sample extraction, enrichment, and chromatography separation parameters. The proposed method was further validated with specific and precise results for the intended use.
ANALYTICAL METHODS
(2021)
Article
Biochemistry & Molecular Biology
Ruth Kudla, Jochen S. Gutmann, Larisa A. Tsarkova
Summary: Dynamic tensiometry is a high-potential analytical tool for assessing physico-chemical characteristics of fragrance molecules. It is sensitive to the composition of fragrances and can be used for detecting purity of essential oils, optimizing compositions, and assessing release/evaporation of fragrances.
Article
Biochemistry & Molecular Biology
Gabriel Onn Kit Loh, Emily Yii Ling Wong, Yvonne Tze Fung Tan, Siew Chyee Heng, Mardiana Saaid, Kit Yee Cheah, Nurul Diyana Mohd Sali, Nair Damenthi, Sharon Shi Min Ng, Long Chiau Ming, Kok Khiang Peh
Summary: A sensitive, fast, and high-throughput HPLC-ESI-MS/MS method was developed to measure etoricoxib levels in human plasma using a one-step methanol protein precipitation technique. The method was found to be suitable for high throughput analysis, with good reproducibility and stability.
Article
Biochemistry & Molecular Biology
Ana Teresa Juarez-Facio, Violaine Martin de Lagarde, Christelle Monteil, Jean-Marie Vaugeois, Cecile Corbiere, Tiphaine Rogez-Florent
Summary: A new HPLC method for the simultaneous quantification of ATP, ADP, and AMP has been developed and validated, allowing for their measurement in different cell models. The method demonstrates linear calibration curves, selectivity, precision, and accuracy, providing a sensitive, simple, and green analytical alternative.
Article
Biochemical Research Methods
Gabriel Onn Kit Loh, Emily Yii Ling Wong, Yvonne Tze Fung Tan, Loke Meng Ong, Ru Shing Ng, Hong Chin Wee, Kok Khiang Peh
Summary: A simple, fast, and sensitive LC-MS/MS method was developed for quantifying terazosin in human plasma, with prazosin used as an internal standard. The method showed high precision and accuracy, with a linear calibration curve from 1.0 to 100.0 ng/mL. The recovery of analyte and internal standard was also found to be high, demonstrating the stability of terazosin in various storage conditions. The method was successfully applied in a bioequivalence study measuring 935 samples.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Chemistry, Analytical
Soon Uk Chae, Ji Seon Kim, Chae Bin Lee, Seong Jun Jo, Kyung Lok Min, Min Jung Chang, Soo Kyung Bae
Summary: A sensitive and fast LC-MS/MS method was developed to quantify remifentanil in human plasma, and prevent its degradation during analysis. The method involved a simple sample preparation process and a short run time. The method was validated and found to be reliable for quantification of remifentanil in plasma. It was successfully applied to measure plasma levels of remifentanil in intensive-care-unit patients.
Article
Chemistry, Analytical
Xin Xu, Lisa A. Murphy
Summary: A fast and sensitive method was developed for quantification of cannabinoids and cannabinoid metabolites in cattle plasma using liquid chromatography-tandem mass spectrometry. The method demonstrated good accuracy, precision, specificity, and high sensitivity. The study also found that 7-carboxy cannabidiol was the predominant cannabinoid present in the cattle plasma for weeks after the hemp feeding had ended.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Chemistry, Analytical
Fei Cheng, Taiming Zhang, Tao Sun, Yujie Wang, Can Zhou, Hongqiu Zhu, Yonggang Li
Summary: A new method for determining iron in water samples without redox pretreatment was proposed in this study, using molecular absorption spectrophotometry with Nitroso R Salt and CTMAB as ligands. The method showed high selectivity and accuracy.
MICROCHEMICAL JOURNAL
(2021)
Article
Engineering, Environmental
Zi-yi Yuan, Tian-yu Li, Jian-feng Zhang, Xiao-mao Wang
Summary: This study aims to refine the 8-HQ complexation-fluorescence method for the quantification of active aluminum in water. The interference caused by hardness ions and natural organic matter (NOM) was eliminated to improve the accuracy of measurement. Magnesium ions were found to cause a substantial positive interference, while NOM had negligible interference. Lowering the buffering pH or using a masking ligand effectively alleviated the interference caused by magnesium. The refined method has a low quantification limit, a wide linear range of measurement, and good precision.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Multidisciplinary Sciences
N. Bruna, B. Collao, A. Tello, P. Caravantes, N. Diaz-Silva, J. P. Monras, N. Ordenes-Aenishanslins, M. Flores, R. Espinoza-Gonzalez, D. Bravo, J. M. Perez-Donoso
SCIENTIFIC REPORTS
(2019)
Article
Chemistry, Medicinal
Manuel Osorio, Ma Angeles Cabrera, Fernando Gonzalez-Nilo, Jose M. Perez-Donoso
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2019)
Article
Microbiology
Carla Gallardo-Benavente, Ornella Carrion, Jonathan D. Todd, Joana C. Pieretti, Amedea B. Seabra, Nelson Duran, Olga Rubilar, Jose M. Perez-Donoso, Andres Quiroz
FRONTIERS IN MICROBIOLOGY
(2019)
Article
Environmental Sciences
Ma Angeles Cabrera, Sebastian L. Marquez, Carolina P. Quezada, Manuel Osorio, Eduardo Castro-Nallar, Fernando D. Gonzalez-Nilo, Jose M. Perez-Donoso
ENVIRONMENTAL POLLUTION
(2020)
Article
Biotechnology & Applied Microbiology
N. Oetiker, C. Munoz-Villagran, C. C. Vasquez, D. Bravo, J. M. Perez-Donoso
Summary: This study aimed to investigate the phototoxicity of biomimetic QDs on Escherichia coli cells, revealing that light exposure increased the toxicity of QDs in cells, especially green-QDs(500). The phototoxicity of QDs in bacteria is mainly associated with the generation of reactive oxygen species (ROS).
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
V Carrasco, V Amarelle, S. Lagos-Moraga, C. P. Quezada, R. Espinoza-Gonzalez, R. Faccio, E. Fabiano, J. M. Perez-Donoso
Summary: Microbes can be found in almost every environment on Earth, including Antarctic lithobiontic bacterium capable of synthesizing cadmium sulfide quantum dots (CdS QDs) and potentially applying them in solar cells.
MICROBIAL CELL FACTORIES
(2021)
Article
Genetics & Heredity
Carla Gallardo-Benavente, Jessica L. Campo-Giraldo, Juan Castro-Severyn, Andres Quiroz, Jose M. Perez-Donoso
Summary: The study presents the draft genome sequence of Pseudomonas sp. GC01, a cadmium-resistant Antarctic bacterium capable of biosynthesizing CdS quantum dots. Through comparative genomic analysis, genes related to cadmium resistance, sulfur metabolism, and MeSH-dependent CdS QDs bioproduction were identified. This work provides insights into the potential genes involved in Cd resistance, sulfur metabolism, and MeSH-dependent CdS QDs bioproduction in Pseudomonas spp. strains.
Article
Chemistry, Multidisciplinary
Osvaldo Yanez, Manuel Isaias Osorio, Eugenio Uriarte, Carlos Areche, William Tiznado, Jose M. Perez-Donoso, Olimpo Garcia-Beltran, Fernando Gonzalez-Nilo
Summary: This study utilized computational models to evaluate potential inhibitors of the SARS-CoV-2 enzyme M-pro, identifying compounds with high affinity that could serve as promising candidates for therapeutic development.
FRONTIERS IN CHEMISTRY
(2021)
Article
Medicine, Research & Experimental
Osvaldo Yanez, Manuel Isaias Osorio, Carlos Areche, Alejandro Vasquez-Espinal, Jessica Bravo, Angelica Sandoval-Aldana, Jose M. Perez-Donoso, Fernando Gonzalez-Nilo, Maria Joao Matos, Edison Osorio, Olimpo Garcia-Beltran, William Tiznado
Summary: Cocoa beans contain antioxidant molecules that have the potential to inhibit the type 2 coronavirus (SARS-CoV-2) that causes COVID-19. In silico tests were conducted to evaluate 30 molecules from cocoa, with isorhoifolin and rutin showing strong binding ability. This research provides a promising starting point for future experiments to validate these molecules as inhibitors of SARS-CoV-2 protease.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Chemistry, Multidisciplinary
C. Arriaza-Echanes, J. L. Campo-Giraldo, C. P. Quezada, R. Espinoza-Gonzalez, P. Rivas-Alvarez, M. Pacheco, D. Bravo, J. M. Perez-Donoso
Summary: In this study, a novel one-step biomimetic method for the aqueous synthesis of CuInS(2) (CIS) nanoparticles was reported. The CIS nanoparticles produced by this method exhibit fluorescence emission, a determined size between 10 and 15 nm, crystalline structure confirmed by X-ray diffraction analysis, and a composition ratio of Cu, In, and S as 0.6:1.4:2. The CIS nanoparticles showed no cytotoxicity in human cells and bacteria, and have potential applications as photosensitizers in quantum dots sensitized solar cells.
ARABIAN JOURNAL OF CHEMISTRY
(2021)
Article
Microbiology
Brief Note Javiera Vera-Mansilla, Cecilia A. Silva-Valenzuela, Patricio Sanchez, Roberto C. Molina-Quiroz
Summary: This study found that the joint administration of antibiotics and phages can effectively eradicate persister cells and impact the viability of biofilm-forming cells, providing a feasible approach for the prevention and treatment of chronic urinary infections.
RESEARCH IN MICROBIOLOGY
(2023)
Article
Microbiology
Javiera Vera-Mansilla, Patricio Sanchez, Cecilia A. Silva-Valenzuela, Roberto C. Molina-Quiroz
Summary: Urinary tract infections (UTIs) are a common bacterial infection, with Escherichia coli being the main cause. Antibiotic-resistance in UTIs has led to the exploration of alternative therapies, such as bacteriophages. In this study, three novel bacteriophages were isolated and characterized, showing efficient infection and killing of laboratory and multidrug-resistant clinical E. coli strains. These phages also demonstrated the ability to infect intestinal pathogenic E. coli strains. This research suggests that these phages may serve as a potential alternative treatment for antibiotic-resistant E. coli strains causing UTIs and intestinal infections.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Alejandro Gran-Scheuch, Javiera Ramos-Zuniga, Edwar Fuentes, Denisse Bravo, Jose M. Perez-Donoso
Article
Microbiology
Roberto C. Molina-Quiroz, Triana N. Dalia, Andrew Camilli, Ankur B. Dalia, Cecilia A. Silva-Valenzuela
Article
Microbiology
Rodrigo L. Ortiz, Felipe Melis-Arcos, Paulo C. Covarrubias, Juan A. Ugalde, Zachary S. Apte, Jose Perez-Donoso, Juan P. Cardenas, Daniel E. Almonacid
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2020)
