4.6 Article

Impact of Molecular Hydrogen on Chalcopyrite Bioleaching by the Extremely Thermoacidophilic Archaeon Metallosphaera sedula

Journal

APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 76, Issue 8, Pages 2668-2672

Publisher

AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.02016-09

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Funding

  1. NCSU Molecular Biotechnology Training Program through an NIH T32 Biotechnology Traineeship
  2. NSF Biotechnology Program
  3. U.S. Defense Threat Reduction Agency (DTRA)

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Hydrogen served as a competitive inorganic energy source, impacting the CuFeS2 bioleaching efficiency of the extremely thermoacidophilic archaeon Metallosphaera sedula. Open reading frames encoding key terminal oxidase and electron transport chain components were triggered by CuFeS2. Evidence of heterotrophic metabolism was noted after extended periods of bioleaching, presumably related to cell lysis.

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