Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 76, Issue 6, Pages 1996-2001Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.01919-09
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Funding
- U.S. Defense Threat Reduction Agency (DTRA) [GG-13]
- IC [HM15820612010]
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The Ibis T5000 is a novel diagnostic platform that couples PCR and mass spectrometry. In this study, we developed an assay that can identify all known pathogenic Vibrio species and field-tested it using natural water samples from both freshwater lakes and the Georgian coastal zone of the Black Sea. Of the 278 total water samples screened, 9 different Vibrio species were detected, 114 (41%) samples were positive for V. cholerae, and 5 (0.8%) samples were positive for the cholera toxin A gene (ctxA). All ctxA-positive samples were from two freshwater lakes, and no ctxA-positive samples from any of the Black Sea sites were detected.
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