4.6 Article

Stable, Site-Specific Fluorescent Tagging Constructs Optimized for Burkholderia Species

Journal

APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 76, Issue 22, Pages 7635-7640

Publisher

AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.01188-10

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Funding

  1. National Institutes of Health [R21-AI074608]
  2. NSF [0549514]

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Several vectors that facilitate stable fluorescent labeling of Burkholderia pseudomallei and Burkholderia thailandensis were constructed. These vectors combined the effectiveness of the mini-Tn7 site-specific transposition system with fluorescent proteins optimized for Burkholderia spp., enabling bacterial tracking during cellular infection.

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