Article
Multidisciplinary Sciences
Tao-Tao Chen, Yanling Lin, Shijun Zhang, Shuxin Liu, Lei Song, Wenhong Zhong, Zhao-Qing Luo, Aidong Han
Summary: The intracellular bacterial pathogen Legionella pneumophila hijacks host vesicular transport factor p115 to localize its effector protein LegA15 in host lipid droplets (LDs) and causes fragmentation of host Golgi apparatus. LegA15 is an atypical GTPase that modulates host LD homeostasis through its GTPase activity.
Review
Cell Biology
Yi Yang, Ligang Mei, Jing Chen, Xiaorong Chen, Zhuolin Wang, Lu Liu, Aimin Yang
Summary: This article summarizes the posttranslational modifications of host proteins mediated by Legionella pneumophila effectors, including phosphorylation, ubiquitination, glycosylation, AMPylation, phosphocholination, methylation, ADP-ribosylation, as well as dephosphorylation, deubiquitination, deAMPylation, deADP-ribosylation, dephosphocholination, and delipidation. The molecular mechanisms and biological functions of these modifications in bacterial growth, Legionella-containing vacuole biosynthesis, and disruption of host immune and defense machinery are described.
JOURNAL OF MOLECULAR CELL BIOLOGY
(2023)
Article
Biology
Hanne Griem-Krey, Carola Petersen, Inga K. Hamerich, Hinrich Schulenburg
Summary: The microbiota plays a crucial role in shaping host biology, including protection against pathogens. This study investigates the triangular interaction between a nematode, a protective symbiont, and a pathogen, and finds that immunity has a greater influence on the fitness of the host-microbe assemblage than microbial colonization. However, the presence of the protective microbe still improves fitness in most cases.
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Sun Cheol Park, So Yeon Cho, Tae Hee Kim, Kyung Yeol Ko, Wan Seok Song, Seung Goo Kang, Geun-Shik Lee, Sung-il Yoon
Summary: LegK7 is an atypical kinase that activates through interaction with the host MOB1 protein without the need for phosphorylation. Upon binding with MOB1, LegK7 undergoes a structural reshaping that allows it to adopt a closed conformation compatible with ATP binding.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Patricia Martorell, Beatriz Alvarez, Silvia Llopis, Veronica Navarro, Pepa Ortiz, Nuria Gonzalez, Ferran Balaguer, Antonia Rojas, Empar Chenoll, Daniel Ramon, Marta Tortajada
Summary: This study demonstrated the functional properties of heat-treated non-viable Bifidobacterium longum CECT-7347, highlighting its potential as a novel postbiotic with protective effects against oxidative stress, inflammatory response, gut-barrier disruption, and bacterial colonization. These findings pave the way for broader applications of HT-CECT-7347 in gut health.
Article
Multidisciplinary Sciences
Cheng-Rung Huang, Cheng-Ju Kuo, Chih-Wen Huang, Yu-Ting Chen, Bang-Yu Liu, Chung-Ta Lee, Po-Lin Chen, Wen-Tsan Chang, Yun-Wen Chen, Tzer-Min Lee, Hui-Chen Hsieh, Chang-Shi Chen
Summary: Enterohemorrhagic Escherichia coli (EHEC) induces attaching and effacing lesions in the intestine through a host signaling pathway involving cyclin-dependent kinase CDK1 and formin CYK1, as demonstrated using human intestinal cells and a C. elegans model of infection.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Tin Tin Manh Nguyen, Van-Hieu Mai, Han Sun Kim, Doyeon Kim, Munjun Seo, Yong Jin An, Sunghyouk Park
Summary: In this study, a fluorine NMR-based method was developed to quantitatively estimate the metabolic interaction between host and gut microbiome. The experimental results revealed the contributions of microbial species with different genetic backgrounds to host drug metabolism, as well as the differential drug metabolism among bacterial substrains and their contributions to host metabolism, which were not observable by conventional sequencing methods. These metabolic contributions were correlated with the developmental toxicity of the drug to the host fed with different substrains.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Biochemistry & Molecular Biology
Kun Wang, Jingchen Sun, Jing Zhao, Yongjiao Gao, Di Yao, Daqing Sun, Mengdie Tai, Yuxi Pan, Yanjie Wang, Baoxin Lu, Feng Zuo
Summary: In this study, a capsular polysaccharide CPS-2 was isolated from Lactobacillus fermentum GBJ and characterized. CPS-2 exhibited immunomodulatory effects and anti-aging properties, making it a potential ingredient in the food or pharmaceutical industry.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Editorial Material
Biochemistry & Molecular Biology
Tong Pan, Elizabeth A. Ronan, X. Z. Shawn Xu
Summary: A new study shows that the heat-sensing neuron AFD is responsible for attracting the parasitic nematode Strongyloides stercoralis to human body heat, using unknown neural mechanisms. Interestingly, this same neuron also plays a role in thermotaxis in the nematode C. elegans.
Article
Pharmacology & Pharmacy
Ying Wang, Qiong Cheng, Qina Su, Xuesong Yu, Tianqi Shen, Xiaoling Yang, Weizhang Jia
Summary: Aesculin, a major active ingredient in Cortex Fraxini, exhibits antioxidative and neuroprotective properties. It enhances antioxidant defenses, reduces oxidative stress and Aβ-induced neurotoxicity, and improves stress resistance in nematodes.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Review
Microbiology
Ayesha Ayesha, Franklin Wang-Ngai Chow, Polly Hang-Mei Leung
Summary: Legionella pneumophila, an intracellular pathogen, utilizes OMVs to communicate with and modulate host immune response, playing a crucial role in disease pathogenesis. Understanding the functions of OMVs provides valuable insights for combating Legionnaires' disease.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Lina Scheithauer, Stefanie Thiem, Can M. Uenal, Ansgar Dellmann, Michael Steinert
Summary: The zinc metalloprotease ProA of L. pneumophila functions as a modulator of flagellin-mediated TLR5 stimulation and is involved in antagonizing immune stimulation by degrading immunogenic FlaA and decreasing TLR5 response. This study shows the importance of ProA in L. pneumophila proliferation in mammalian hosts.
Article
Medicine, Research & Experimental
Tomomi Komura, Motoshi Aoki, Satoshi Kotoura, Yoshikazu Nishikawa
Summary: The accumulation of amyloid-beta protein in the brain is a key factor in Alzheimer's disease, and it can be inhibited by certain strains of lactic acid bacteria (LAB). In this study, two strains of LAB, Lactococcus laudensis (LL) and Pediococcus parvulus (PP), were found to prevent the toxicity of A beta accumulation and alleviate neurotoxicity in AD model worms. This research suggests that LAB could be a potential preventive method for Alzheimer's disease.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Review
Biochemistry & Molecular Biology
Yury Belyi, Nadya Levanova, Gunnar N. Schroeder
Summary: Work over the past two decades has revealed the significant role of glycosyltransferase effectors in the infection strategy of Legionella pneumophila, a Gram-negative respiratory pathogen. These effectors manipulate host protein synthesis, impacting the physiological state of target cells, such as cell cycle progression and immune and stress responses. Recent research has identified new glycosyltransferases and suggests the existence of undiscovered family members with unique catalytic features and substrates.
Article
Immunology
Kohar Annie B. Kissoyan, Lena Peters, Christoph Giez, Jan Michels, Barbara Pees, Inga K. Hamerich, Hinrich Schulenburg, Katja Dierking
Summary: The effects of the natural microbiota on the physiology of Caenorhabditis elegans are still not fully understood. This study found that certain microbiota members affect worm lifespan, fertility, and intestinal colonization, highlighting the blurred line between beneficial and harmful microorganisms.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Physiology
Eriko Kage-Nakadai, Simo Sun, Satoru Iwata, Sawako Yoshina, Yoshikazu Nishikawa, Shohei Mitani
JOURNAL OF PHYSIOLOGICAL SCIENCES
(2019)
Article
Agriculture, Dairy & Animal Science
S. N. Magray, S. A. Wani, Z. A. Kashoo, M. A. Bhat, S. Adil, S. Farooq, M. A. Rather, Z. A. Kabli, M. T. Banday, Y. Nishikawa
ANIMAL PRODUCTION SCIENCE
(2019)
Article
Neurosciences
Simo Sun, Akane Ohta, Atsushi Kuhara, Yoshikazu Nishikawa, Eriko Kage-Nakadai
NEUROSCIENCE RESEARCH
(2020)
Article
Biochemistry & Molecular Biology
Chien-Sen Liao, Yong-Han Hong, Yoshikazu Nishikawa, Eriko Kage-Nakadai, Tai-Ying Chiou, Chien-Chang Wu
Article
Geriatrics & Gerontology
Ai Ito, Quichi Zhao, Yoichiro Tanaka, Masumi Yasui, Rina Katayama, Simo Sun, Yoshihiko Tanimoto, Yoshikazu Nishikawa, Eriko Kage-Nakadai
Summary: Metolazone has been identified as a drug that extends the lifespan of C. elegans by activating UPRmt. It upregulates mitochondrial chaperone proteins and has a specific mode of action, as shown in HeLa cell lines.
Article
Biotechnology & Applied Microbiology
R. Katayama, Y. Matsumoto, Y. Higashi, S. Sun, H. Sasao, Y. Tanimoto, Y. Nishikawa, E. Kage-Nakadai
Summary: This study showed that Bacillus subtilis (natto) can enhance the resistance of Caenorhabditis elegans to gram-positive bacteria and increase the survival time of the worms upon infection. Transcriptome analysis revealed the upregulation of genes associated with immunity and defense response in B. subtilis (natto)-fed worms.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Public, Environmental & Occupational Health
Yoshihiko Tanimoto, Miyoko Inoue, Kana Komatsu, Atsuyuki Odani, Takayuki Wada, Eriko Kage-Nakadai, Yoshikazu Nishikawa
Summary: The study found that E. coli expressing K88(O169) adhesin can infect different hosts including pigs and cattle, with the first report of domestic livestock having antibodies to K88(O169) of human ETEC; suggesting zoonotic strains may hide among human ETEC organisms, calling for the adoption of the One Health concept to intervene in ETEC infections among animals and humans.
EPIDEMIOLOGY AND INFECTION
(2021)
Article
Microbiology
Ayano Tsuru, Yumi Hamazaki, Shuta Tomida, Mohammad Shaokat Ali, Tomomi Komura, Yoshikazu Nishikawa, Eriko Kage-Nakadai
Summary: This study investigated the effects of different Cutibacterium acnes strains on the life span of Caenorhabditis elegans and host resistance against Staphylococcus aureus. It was found that certain strains of C. acnes could shorten the life span of C. elegans, while others, like strain HL110PA4, did not alter it. Additionally, it was discovered that the TIR-1 and p38 MAPK pathways play a role in conferring host resistance against S. aureus in a C. acnes-mediated manner.
MICROBIOLOGY SPECTRUM
(2021)
Article
Microbiology
Tomomi Komura, Asami Takemoto, Hideki Kosaka, Toshio Suzuki, Yoshikazu Nishikawa
Summary: This study revealed the anti-aging effects of Lactococcus cremoris subsp. cremoris strain FC through the SKN-1/Nrf2 pathway, and its beneficial effects on perception and inhibition of AGEs in nematodes.
MICROBIOLOGY SPECTRUM
(2022)
Article
Medicine, Research & Experimental
Tomomi Komura, Motoshi Aoki, Satoshi Kotoura, Yoshikazu Nishikawa
Summary: The accumulation of amyloid-beta protein in the brain is a key factor in Alzheimer's disease, and it can be inhibited by certain strains of lactic acid bacteria (LAB). In this study, two strains of LAB, Lactococcus laudensis (LL) and Pediococcus parvulus (PP), were found to prevent the toxicity of A beta accumulation and alleviate neurotoxicity in AD model worms. This research suggests that LAB could be a potential preventive method for Alzheimer's disease.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Geriatrics & Gerontology
Tomomi Komura, Mikihiro Yamanaka, Kohji Nishimura, Keita Hara, Yoshikazu Nishikawa
Summary: Autofluorescence can be used as a noninvasive biomarker of senescence in Caenorhabditis elegans. The fluorescence of individual nematodes increases with age, with worms exhibiting lower fluorescence having longer life expectancy. Glycated substances contribute to the increase in fluorescence, making it a useful biomarker for AGEs.
NPJ AGING AND MECHANISMS OF DISEASE
(2021)
Article
Infectious Diseases
Md Shafiullah Parvej, Md Ashraful Alam, Mio Shono, Nusrat Zahan, Misrat Masuma Parvez, Wahedul Karim Ansari, Md Sayduzzaman Jowel, Md Sharif Uddin, Eriko Kage-Nakadai, Md Tanvir Rahman, Yoshikazu Nishikawa
JAPANESE JOURNAL OF INFECTIOUS DISEASES
(2020)
