Article
Chemistry, Analytical
Mengyun Zhou, Chuan Fan, Lirong Wang, Tailin Xu, Xueji Zhang
Summary: This study reports an integrated microdroplet array platform composed of an ultrasonic unit and minipillar array for enhanced RPA detection of SARS-CoV-2. The independent microvolume reactions and microstreaming driven by ultrasound greatly accelerate the amplification and increase the sensitivity, while reducing reagent consumption.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Ian T. Hull, Enos C. Kline, Gaurav K. Gulati, Jack Henry Kotnik, Nuttada Panpradist, Kamal G. Shah, Qin Wang, Lisa Frenkel, James Lai, Joanne Stekler, Barry R. Lutz
Summary: The study presents a novel quantitative detection method for HIV DNA or RNA using a combination of recombinase polymerase amplification and lateral flow detection technologies, allowing for rapid and accurate testing in low-resource healthcare settings, with the potential to improve the quality of HIV treatment.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Gaihua Cao, Jiangbo Dong, Xiaolong Chen, Peng Lu, Yifan Xiong, Lan Peng, Jiawei Li, Danqun Huo, Changjun Hou
Summary: In this study, a strategy (MPT-Cas12a/13a) was developed that combines CRISPR/Cas12a and Cas13a for simultaneous detection of CaMV35S and T-nos. This strategy utilizes multiplex PCR and transcription to achieve simultaneous detection with different signals in the same space. The MPT-Cas12a/13a showed excellent sensitivity, with detection limits as low as 11 copies of T-nos and 13 copies of CaMV35S, and demonstrated outstanding specificity and anti-interference ability in actual sample analysis. Therefore, it has the potential to be used for the detection of GM crops.
CHEMICAL COMMUNICATIONS
(2022)
Article
Agriculture, Dairy & Animal Science
Yanling Pang, Feng Cong, Xinheng Zhang, Hongxin Li, Yung-Fu Chang, Qingmei Xie, Wencheng Lin
Summary: A probe-based recombinase polymerase amplification assay was developed for rapid detection of Chlamydia psittaci. The test showed high sensitivity and accuracy in field applications, offering a promising approach for screening and monitoring outbreaks in domestic fowl populations.
Article
Food Science & Technology
Ruibin Xie, Juan Zhang, Shuang Wen, Ruiming Zhao, Nan Wang, Ailiang Chen
Summary: This study established a simple and rapid real-time recombinase polymerase amplification (RPA) assay for oilfish and escolar detection. The assay is specific and sensitive, and can help control adulteration and ensure consumer health.
Article
Chemistry, Analytical
Mengnan Fan, Jianru Yang, Xiaosu Wang, Yongjie Xu, Bing Li, Hui Yang, Qin Lu, Xun Min, Meirong Huang, Jian Huang
Summary: Neisseria gonorrhoeae is the only pathogen that causes gonorrhea. A developed detection method based on recombinase polymerase amplification (RPA) can eliminate false-positive signals and has the advantages of rapid performance, high sensitivity, and minimal dependency on equipment.
ANALYTICA CHIMICA ACTA
(2023)
Review
Nanoscience & Nanotechnology
Xinrui Feng, Yan Liu, Yang Zhao, Zhe Sun, Ning Xu, Chen Zhao, Wei Xia
Summary: With the recent outbreaks of new emergency zoonotic diseases, the development of fast, accurate, and portable screening assays for pathogen infections has become urgently needed. Recombinase polymerase amplification (RPA) is a sensitive and specific method that can be conducted at a constant low temperature with a short response time, making it suitable for on-site screening and a powerful tool for preventing or controlling the spread of zoonoses.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2023)
Article
Chemistry, Analytical
Chang Liu, Xuechun Yao, Chunlong Liu, Shengping You, Wei Qi, Mengfan Wang
Summary: The study established and optimized two lateral flow device based molecular assay systems, RPA-NFO-LFT and RPA-Cas12a-LFT, for the detection of Candida albicans (C. albicans) infection. Both systems showed good specificity and sensitivity, with a lower detection limit of 10(3) CFU ml(-1). RPA-Cas12a-LFT demonstrated higher accuracy for visual interpretation and better stability towards samples with serum nucleic acid interference compared to the conventional qPCR method.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Analytical
Rui Ge, Hanjie Dai, Shumin Zhang, Jie Wei, Tianhui Jiao, Qingmin Chen, Quansheng Chen, Xiaomei Chen
Summary: In this study, a photoelectrochemical (PEC) biosensor combined with recombinase polymerase amplification (RPA) technology (RPA-PEC) was proposed for rapid detection of multiple foodborne pathogens. The RPA was initiated on homemade three-dimensional screen-printed paper-based electrodes, forming a lab-on-paper platform. Using the DNA-PEC signaler, photocurrents were achieved after only 20 minutes of RPA, with superior detection performance compared to conventional agarose gel electrophoresis.
ANALYTICAL CHEMISTRY
(2023)
Article
Parasitology
Fuchang Yu, Kaihui Zhang, Yilin Wang, Dongfang Li, Zhaohui Cui, Jianying Huang, Sumei Zhang, Xiaoying Li, Longxian Zhang
Summary: A new strategy for direct identification of a specific subtype of C. parvum has been successfully developed in this study, allowing for faster and more convenient diagnosis without the need for expensive equipment or highly trained operators.
PARASITES & VECTORS
(2021)
Article
Biotechnology & Applied Microbiology
Baicheng Huang, Yufeng Lou, Zihan Zeng, Xingchi Kan, Xinping Shi, Yue Wu, Ling Guo, Muzhen Wang, Xingxu Huang, Xuemei Tian, Xinjie Wang
Summary: A Cas12a-based fluorescent microfluidic detection system was developed for rapid and sensitive detection of six HPV subtypes. The assay showed consistent results with the qPCR assay when tested with clinical samples, holding promise for improving cervical cancer screening and prevention.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Analytical
Fangchi Shao, Joon Soo Park, Guojie Zhao, Kuangwen Hsieh, Tza-Huei Wang
Summary: Developing assays that combine CRISPR/Cas and isothermal nucleic acid amplification is a growing research area, with potential for point-of-care applications. Current research mainly focuses on appending different CRISPR/Cas effectors to isothermal nucleic acid amplification methods, but few studies have integrated both components into single-step assays. We conducted a systematic investigation and found that adding CRISPR/Cas12a enhances signal amplification but hinders recombinase polymerase amplification (RPA). We developed a PAM-free CRISPR/Cas12a-assisted RPA assay that outperforms other assays in design, signal, sensitivity, and speed. Our study provides new insights and can facilitate further advances in CRISPR/Cas-based assays.
ANALYTICAL CHEMISTRY
(2023)
Article
Immunology
Lei Wang, Aiguo Xu, Ping Zhou, Mengdi Zhao, Chenglai Xu, Yan Wang, Kun Wang, Fang Wang, Yongchang Miao, Weiguo Zhao, Xuzhu Gao
Summary: In this study, the researchers developed a reliable molecular diagnostic method using RPA-LFS for the detection of C. tropicalis. The optimized primer-probe design allowed for specific and sensitive detection of the fungus in clinical samples. The results showed that the RPA-LFS system had high specificity and sensitivity, allowing for rapid and accurate detection of C. tropicalis in a short period of time. This method has significant importance for clinical diagnosis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Chemistry, Analytical
Michihiko Nakano, Masafumi Inaba, Junya Suehiro
Summary: This study introduces a rapid and cost-effective method for amplicon detection, where amplicons are attached to magnetic microbeads, suspended in deionized water, and agglomerated in a circular pattern on a hydrophilic surface under a magnetic field, making them visible to the naked eye.
Article
Chemistry, Multidisciplinary
Jungang Du, Biao Ma, Jiali Li, Yaping Wang, Tianyu Dou, Shujuan Xu, Mingzhou Zhang
Summary: This study developed an easy and rapid method to specifically distinguish Legionella pneumophila and non-Legionella pneumophila. The method utilizes recombinase polymerase amplification reaction and EuNPs-based lateral flow immunochromatography, achieving high sensitivity and accurate results.
FRONTIERS IN CHEMISTRY
(2022)
Editorial Material
Immunology
A. Clinton White, Christina M. Coyle
CLINICAL INFECTIOUS DISEASES
(2020)
Article
Public, Environmental & Occupational Health
Gabriel H. Quispe-Rodriguez, Alyssa A. Wankewicz, Jose Luis Malaga Granda, Ben Lewis, Kassi Stockert, A. Clinton White
Article
Biochemistry & Molecular Biology
Samantha Nava, Aygul Sadiqova, Alejandro Castellanos-Gonzalez, A. Clinton White
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
(2020)
Editorial Material
Immunology
A. Clinton White
CLINICAL INFECTIOUS DISEASES
(2021)
Review
Pharmacology & Pharmacy
Beilin Wang, Alejandro Castellanos-Gonzalez, Jr A. Clinton White
EXPERT OPINION ON THERAPEUTIC TARGETS
(2020)
Article
Infectious Diseases
A. Clinton White, Seth O'Neal, Andrea Winkler, Annette Abraham, Helene Carabin
PLOS NEGLECTED TROPICAL DISEASES
(2020)
Article
Infectious Diseases
Annette Abraham, Javier A. Bustos, Helene Carabin, Robert de Meijere, Priyadarshi S. Sahu, Vedantam Rajshekhar, Gagandeep Singh, A. Clinton White, Peter L. Chiodini, Sarah Gabriel, Mamoun Homeida, Theodore Nash, Bernard Ngowi, Xiao Nong Zhou, Christina Coyle, Hector H. Garcia, Andrea S. Winkler
Summary: The study used systematic review and meta-analysis to determine the best anti-seizure medication and anti-inflammatory treatment for individuals with single brain enhancing lesions (SEL) neurocysticercosis (NCC) based on the resolution of cysts. Results showed that prolonging the duration of anti-seizure medication (ASM) in patients with calcified cysts post treatment was beneficial in reducing seizure recurrence, and anti-inflammatory treatment was also found to be beneficial in reducing seizures in these patients.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Parasitology
Catalina Aron-Said, Martin Montes, A. Clinton White, Miguel M. Cabada
Summary: The study found that children with acute fascioliasis had significantly higher levels of IL-5 and IL-17 in their plasma, and the increase in IL-5 was associated with eosinophilia in that group.
PARASITOLOGY RESEARCH
(2021)
Article
Multidisciplinary Sciences
Alejandro Castellanos-Gonzalez, Thomas R. Shelite, Nicole Lloyd, Aygul Sadiqova, Ren Ping, Natalie Williams-Bouyer, Peter C. Melby, Bruno L. Travi
Summary: The COVID-19 pandemic caused by SARS-CoV-2 is still spreading rapidly worldwide, posing challenges for diagnostic laboratories in developing countries due to shortages of kits and supplies. Validating alternative nucleic acid isolation methods for SARS-CoV-2 is urgent to address these issues.
SCIENTIFIC REPORTS
(2021)
Article
Infectious Diseases
Camille M. Webb, Maria Luisa Morales, Martha Lopez, Benicia Baca-Turpo, Eulogia Arque, A. Clinton White, Miguel M. Cabada
Summary: The study found that chronic fascioliasis may be associated with stunting in children, and the severity of the disease is related to factors such as history of malnutrition and anemia treatment.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Parasitology
Julio Guerrero-Hernandez, Raul J. Bobes, Martin Garcia-Varela, Alejandro Castellanos-Gonzalez, Juan P. Laclette
Summary: This study described a gene silencing procedure on cysticerci of the taeniid cestode Taenia crassiceps for the first time, and identified AGO and Dcr orthologues, confirming the presence of gene silencing machinery in T. crassiceps. Experimental results demonstrated that gene silencing using siRNA can be used as a molecular methodology to study gene function in taeniid cestodes.
Article
Biochemical Research Methods
Thomas R. Shelite, Ashanti C. Uscanga-Palomeque, Alejandro Castellanos-Gonzalez, Peter C. Melby, Bruno L. Travi
Summary: The rapid development of an RPA-LF testing method has shown successful detection of SARS-CoV-2, with high accuracy and potential for use in resource-limited and decentralized laboratories.
JOURNAL OF VIROLOGICAL METHODS
(2021)
Article
Public, Environmental & Occupational Health
Bruno L. Travi, Maxy B. Delos Santos, Thomas R. Shelite, Rocio P. Santos, Luis A. Rosales, Alejandro Castellanos-Gonzalez, Omar Saldarriaga, Peter C. Melby
Summary: Cutaneous leishmaniasis is highly prevalent in rural and sylvatic regions of Latin America, with diagnosis still relying on microscopy in resource-limited areas. Recombinase-polymerase amplification (RPA-LF) shows potential as a point-of-care test, but further simplification and optimization will be needed for broader applicability.
VECTOR-BORNE AND ZOONOTIC DISEASES
(2021)
Review
Immunology
Ewa Tomczak, April N. McDougal, A. Clinton White
Summary: Cryptosporidium is a major cause of diarrheal disease worldwide, particularly in malnourished children and immunocompromised patients. The current treatment options are not consistently effective, and no controlled trials have been conducted. However, combination therapies may hold promise for improving treatment outcomes.
OPEN FORUM INFECTIOUS DISEASES
(2022)
Article
Immunology
A. Castellanos-Gonzalez, A. Sadiqova, J. Ortega-Mendez, A. C. White
Summary: Cryptosporidium is a major cause of diarrhea in children and can be fatal for children under 5 years old. Current treatment options, such as Nitazoxanide, have limited efficacy in certain patient populations, calling for the development of novel therapies. This study proposes a novel approach using RNA interference to silence essential genes in Cryptosporidium, showing promising results in reducing parasite burden both in vitro and in vivo. The findings highlight the potential of antisense RNA-based therapy as an alternative treatment for cryptosporidiosis.
INFECTION AND IMMUNITY
(2022)
