Article
Fisheries
Jayasundara Mudiyanselage Sajani Nisansala Rathnapala, Wafaa Ragab, Satoshi Kawato, Miho Furukawa, Reiko Nozaki, Hidehiro Kondo, Ikuo Hirono
Summary: By sequencing the genomes of three strains of Vibrio nigripulchritudo, it was found that they possess various virulence factors and mobile genetic elements, as well as multidrug resistance. Comparative genomic analysis showed that all three strains belong to the same clade.
JOURNAL OF FISH DISEASES
(2023)
Article
Fisheries
Hsin-Wei Kuo, Li-Yang Hsu, Winton Cheng
Summary: Octopamine/Tyramine receptor plays an important role in the immune response of white shrimp, Litopenaeus vannamei. Its expression is upregulated under Vibrio alginolyticus infection and is functionally responsible for the observed immune response.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Microbiology
Yi-Ting Chang, Hao-Ting Ko, Ping-Lun Wu, Ramya Kumar, Han-Ching Wang, Hsiao-Pei Lu
Summary: This study revealed that shrimp gut microbiota responded differently to pathogenic and non-pathogenic Vibrio parahaemolyticus strains, with pathogenic Vibrio-infected shrimp showing enrichment of Photobacterium and Vibrio genera, while non-pathogenic Vibrio-infected shrimp had Candidatus Bacilliplasma enriched. Functional predictions suggested that changes in taxonomic compositions would affect normal metabolic functions, highlighting the importance of maintaining gut microbiota equilibrium. Several biomarkers associated with specific microbial taxa and functional pathways were identified, aiding in predicting disease outcomes.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Yi-Ting Chang, Hao-Ting Ko, Ping-Lun Wu, Ramya Kumar, Han-Ching Wang, Hsiao-Pei Lu
Summary: This study reveals the different responses of shrimp gut microbiota to pathogenic and non-pathogenic Vp strains, with bacterial genera Photobacterium and Vibrio enriched in pathogenic Vp-infected shrimp, and Candidatus Bacilliplasma enriched in non-pathogenic Vp-infected shrimp. Moreover, changes in taxonomic compositions further affect normal metabolic functions, emphasizing the importance of sustaining an equilibrium in the gut microbiota. Several biomarkers associated with specific microbial taxa and functional pathways were identified, which help predict the incidence of disease outcomes.
MICROBIOLOGY SPECTRUM
(2023)
Article
Immunology
Muneeswaran Thillaichidambaram, Kalyanaraman Narayanan, Sureshkumar Selvaraj, Sathyavathi Sundararaju, Ramakritinan Chockalingam Muthiah, Marian J. Figge
Summary: This study investigates the pathogenicity of Vibrio owensii on Litopenaeus vannamei post larvae and finds that the strain lacks major virulence factors, resulting in no pathogenicity.
MICROBIAL PATHOGENESIS
(2022)
Article
Immunology
Xianliang Zhao, Jie Qiao, Pei Zhang, Zehui Zhang, Jude Juventus Aweya, Xiaohan Chen, Yongzhen Zhao, Yueling Zhang
Summary: In this study, the proteomics approach was used to characterize the hemocyanin expression profiles from Litopenaeus vannamei, revealing molecular diversity at the protein level. The research demonstrated that the glycan modification may contribute to the diversity and multiple immune activities of hemocyanin, which plays a crucial role in recognizing different pathogens.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Fisheries
Yusuf Jibril Habib, Haifu Wan, Yulong Sun, Jialong Shi, Chengjie Yao, Jiaming Lin, Hui Ge, Yilei Wang, Ziping Zhang
Summary: This study conducted a genome-wide study of Toll-like receptor genes in Pacific white shrimps for the first time, identifying 11 TLR genes. The majority of these genes were found to be ubiquitously expressed in various tissues and significantly upregulated at different time points after infection, suggesting their significant roles in the immune response of Litopenaeus vannamei.
Article
Microbiology
James W. Conrad, Valerie J. Harwood
Summary: This study reveals that sewage can significantly increase the density of Vibrio vulnificus populations and affect the transcription of genes associated with virulence, suggesting an increased risk of V. vulnificus infections.
MICROBIOLOGY SPECTRUM
(2022)
Article
Fisheries
Guowei Liao, Qiuping Wu, Binhua Mo, Jiang Zhou, Junyi Li, Jixing Zou, Lanfen Fan
Summary: This study investigated the impact of Vibrio alginolyticus on the intestinal histomorphology and microbiome composition in Litopenaeus vannamei. The results showed that Vibrio alginolyticus infection caused exfoliation of intestinal mucosal epithelial cells and altered the tissue morphology of the intestine. It also affected the abundance and diversity of intestinal microbiome.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Environmental Sciences
Davide Albanese, Christian Cainelli, Valeria Gualandri, Simone Larger, Massimo Pindo, Claudio Donati
Summary: This study reports the genome sequences of 82 isolates of E. amylovora collected from different host plants in the Trentino region of the Italian Alps over a 16-year period. The study reveals limited genomic diversity of the isolates and a strong correlation between geography and phylogenetic structure. Furthermore, a novel subclade composed of isolates sampled after 2018 is identified.
ENVIRONMENTAL MICROBIOLOGY REPORTS
(2022)
Article
Fisheries
Hongtao Liu, Shengtao Guo, Rong Wang, Yugui He, Qiong Shi, Zhaobin Song, Mingqiu Yang
Summary: The study used proteomic sequencing of hepatopancreas in Vibrio harveyi-infected whiteleg shrimp and found a significant number of DEPs, specifically showing changes in the expression levels of C-type lectins after infection. Different members of the CTL family exhibit varied responses to Vibrio harveyi infection, suggesting potential differential functions and providing theoretical guidance for further research on immune responses and pathogen infections in shrimps.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Immunology
Kattapuni Suresh Prithvisagar, Ballamoole Krishna Kumar, Toshio Kodama, Praveen Rai, Tetsuya Iida, Iddya Karunasagar, Indrani Karunasagar
Summary: This study analyzed the genomes of Vibrio parahaemolyticus isolates from moribund shrimps in India, revealing significant genetic diversity without the classical PirAB features associated with the AHPND strains. The presence of various virulence attributes, including toxins and secretion systems, highlights the potential evolution of environmental V. parahaemolyticus into pathogens of aquaculture species. Monitoring of V. parahaemolyticus genomes is necessary to study the emergence of new variants in shrimp aquaculture and implement biosecurity measures to prevent disease outbreaks.
Article
Immunology
Fan Mao, Kunna Liu, Nai-Kei Wong, Xiangyu Zhang, Wenjie Yi, Zhiming Xiang, Shu Xiao, Ziniu Yu, Yang Zhang
Summary: The study demonstrates that hosts exhibit different immune responses when encountering Vibrio pathogens of varying virulence, including activation of different immune pathways. Highly virulent Vibrio strains induce apoptosis in oyster cells, while less virulent strains enhance phagocytosis and cell adhesion capabilities.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Mario Lopez-Perez, Jane M. Jayakumar, Trudy-Ann Grant, Asier Zaragoza-Solas, Pedro J. Cabello-Yeves, Salvador Almagro-Moreno
Summary: Pathogen emergence is a complex phenomenon that is poorly understood, particularly in terms of the genetic traits and environmental factors that contribute to the pathogenic potential of specific strains. The distribution of unique genetic markers and clusters in different ecological locations highlights the influence of biotic and abiotic factors in shaping pathogen emergence. Understanding these relationships can aid in assessing and preventing future outbreaks.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Immunology
Mediha Yildirim-Aksoy, Rashida Eljack, Eric Peatman, Benjamin H. Beck
Summary: This study evaluated the changes in hematology, immunology, and biochemical values in the hemolymph of shrimp infected with Vibrio parahaemolyticus. The results showed that shrimp's immune response was altered, and the hemolymph parameters could be used to assess the health status of shrimp.
MICROBIAL PATHOGENESIS
(2022)
Article
Engineering, Biomedical
Julien Duboisset, Patrick Ferrand, Arthur Baroni, Tilman A. Grunewald, Hamadou Dicko, Olivier Grauby, Jeremie Vidal-Dupiol, Denis Saulnier, Le Moullac Gilles, Martin Rosenthal, Manfred Burghammer, Julius Nouet, Corinne Chevallard, Alain Baronnet, Virginie Chamard
Summary: Biomineralization is a complex process bio-controlled by living organisms, involving physical and chemical processes such as ionic concentration regulation and organic molecules production. This study investigates the early-mineralized calcareous units from two bivalve species using advanced optical and X-ray microscopy methods, revealing chemical and crystallographic insights. The observed ring-like structural features in calcite units are correlated with the absence of calcite and an increase in amorphous calcium carbonate and proteins content. Based on these findings, a temporal biomineralization cycle is proposed, providing biomimetic perspectives for designing crystalline materials.
ACTA BIOMATERIALIA
(2022)
Article
Mathematical & Computational Biology
David Couvin, Inaki Cervera-Marzal, Audrey David, Yann Reynaud, Nalin Rastogi
Summary: Limited data on bovine tuberculosis and related infections are available. A publicly accessible database, SITVITBovis, has been constructed, providing genotyping and epidemiological data on Mycobacterium bovis and Mycobacterium caprae. With information on thousands of isolates from around the world, it allows for a wider understanding of the geographic distribution and phylogenetical sublineages. The database also includes molecular marker information and facilitates crosscheck among databases.
DATABASE-THE JOURNAL OF BIOLOGICAL DATABASES AND CURATION
(2022)
Article
Geochemistry & Geophysics
Jean-Pierre Cuif, Yannicke Dauphin, Cedrik Lo, Kadda Medjoubi, Denis Saulnier, Andrea Somogyi
Summary: By using synchrotron-based X-ray fluorescence, the early post-grafting mineral deposits in Polynesian pearls have been studied with unprecedented accuracy. This study reveals that the grafting process can result in a greater degradation and evaluates the possible changes in the biomineralization mechanism during grafting.
Article
Microbiology
Pierre Lopez, Denis Saulnier, Shital Swarup-Gaucher, Rarahu David, Christophe Lau, Revahere Taputuarai, Corinne Belliard, Caline Basset, Victor Labrune, Arnaud Marie, Jean Francois Bernardet, Eric Duchaud
Summary: The orbicular batfish, the most important marine fish reared in French Polynesia, has been facing severe outbreaks of "white-patch disease" since 2011, threatening the sustainability of its aquaculture. The disease has been linked to the pathogen T. maritimum, and there is genetic diversity among the isolates, which is crucial for epidemiological studies and the development of management and control strategies.
Article
Biochemical Research Methods
Vincent Moco, Damien Cazenave, Maelle Garnier, Matthieu Pot, Isabel Marcelino, Antoine Talarmin, Stephanie Guyomard-Rabenirina, Sebastien Breurec, Severine Ferdinand, Alexis Dereeper, Yann Reynaud, David Couvin
Summary: The study aims to promote the use of public repositories in a programmatic way and simplify sequence data analysis from a pedagogical perspective. The getSequenceInfo software tool allows access to sequence information from various public databases and is compatible with different operating systems. Additional tools have also been developed for various sequence analysis tasks.
BMC BIOINFORMATICS
(2022)
Article
Infectious Diseases
Alexandre Bourles, Anne Tristan, Francois Vandenesch, Michele Bes, Frederic Laurent, Anne-Gaelle Ranc, Malia Kainiu, Ann-Claire Gourinat, Antoine Biron, Cecile Cazarola, Cyrille Goarant, Julien Colot
Summary: This study investigated the epidemiology of MRSA in New Caledonia and identified a low diversity with the dominance of a clonal complex not reported previously. The recommendation to stop the topical use of fusidic acid was made to control the emergence of this severe MRSA clone.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2022)
Article
Microbiology
Evelyne Krin, Zeynep Baharoglu, Odile Sismeiro, Hugo Varet, Jean-Yves Coppee, Didier Mazel
Summary: In this study, additional type II and type I Toxin/Antitoxin (TA) systems were identified in the Vibrio cholerae N16961 genome. The functional type II TA system was encoded by the VCA0497-0498 operon, while the novel type I TA system was encoded by the VCA0495 gene and its associated antisense non-coding RNAs. These findings increase the number of TA systems in the genome and highlight their importance in this large cassette array.
RESEARCH IN MICROBIOLOGY
(2023)
Article
Biochemical Research Methods
Rocio Lopez-Igual, Pedro Dorado-Morales, Didier Mazel
ACS SYNTHETIC BIOLOGY
(2023)
Article
Microbiology
Murielle Chauvel, Sophie Bachellier-Bassi, Anne-Marie Guerout, Keunsook K. Lee, Corinne Maufrais, Emmanuelle Permal, Juliana Pipoli Da Fonseca, Sadri Znaidi, Didier Mazel, Carol A. Munro, Christophe d'Enfert, Melanie Legrand
Summary: Despite the complete sequencing of the genome of Candida albicans over two decades ago, there are still over 4300 uncharacterized genes. In this study, a collection of over 2500 overexpression mutants was constructed and evaluated to identify genes with fitness costs when overexpressed. This new resource enables routine large-scale screens under different growth conditions and facilitates the characterization of gene functions in C. albicans.
RESEARCH IN MICROBIOLOGY
(2023)
Article
Microbiology
Leticia Larotonda, Damien Mornico, Varun Khanna, Joaquin Bernal-Bayard, Jean-Marc Ghigo, Marie-Eve Val, Diego Comerci, Didier Mazel, Alfonso Soler-Bistue
Summary: The location of ribosomal protein genes on the bacterial chromosome conditions the evolutionary trajectory of Vibrio cholerae. Moving these genes away from the replication origin reduces growth rate, fitness, and infectivity. No mutation can compensate for the growth defect caused by gene relocation.
Article
Infectious Diseases
Alexandre Bourles, Malia Kainiu, Damaris Ukeiwe, Nina Brunet, Camille Despaux, Antoine Biron, Ann-Claire Gourinat, Cyrille Goarant, Julien Colot
Summary: This study proposes a culture-based method with ertapenem selection to distinguish non-CRE, non-CP-CRE, and CP-CRE from samples with high bacterial loads. The method was successfully applied to clinical and environmental samples, allowing the identification of CP-CRE and detection of carbapenemase. This cost-effective strategy provides fast and reliable results and could be beneficial in resource-limited settings.
Article
Genetics & Heredity
Matthieu Pot, Celia Ducat, Yann Reynaud, David Couvin, Severine Ferdinand, Sebastien Breurec, Antoine Talarmin, Stephanie Guyomard-Rabenirina
Summary: This study reports the first de novo assembled and annotated whole-genome sequence of an E. chengduensis strain isolated from the environment, providing a useful resource for further analysis of this rarely reported Enterobacter species.
Review
Biochemistry & Molecular Biology
Pedro Dorado-Morales, Morgan Lamberioux, Didier Mazel
Summary: In recent years, there has been a notable increase in multidrug-resistant pathogens, which poses a challenge to antibiotic-based medicine. In order to prevent dysbiosis in treated patients, there is a need for novel treatments that can specifically target microorganisms without disturbing other species. Conjugation, a process that transfers DNA from one bacterium to another, has been suggested as a method to modify the microbiome and has gained attention for its potential in gene delivery studies.
MOLECULAR MICROBIOLOGY
(2023)
Review
Microbiology
Marion Desdouits, Yann Reynaud, Cecile Philippe, Francoise S. Le Guyader
Summary: This review discusses the role of bivalve molluscan shellfish in human diseases and highlights the diversity of bacteria or enteric viruses detected in shellfish species. It also discusses the significance of technological developments using genomics to anticipate the emergence of pathogens.
Review
Microbiology
Manon Lang, Andre Carvalho, Zeynep Baharoglu, Didier Mazel
Summary: This review aims to consolidate existing and recent knowledge about the uptake of aminoglycosides (AGs), explore the connection between AG-dependent bacterial stress and drug uptake, and present new strategies to enhance AG uptake for more efficient antibiotic therapies, with a focus on the connection between sugar transport and AG potentiation.
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS
(2023)
