Review
Biochemistry & Molecular Biology
Hugo Guerin, Saulius Kulakauskas, Marie-Pierre Chapot-Chartier
Summary: Rha-CWPSs are crucial cell wall components of many important bacteria, playing a critical role in bacterial growth, division, and interactions with phages or hosts. This review summarizes the current knowledge on the structure, biosynthesis, and contribution of Rha-CWPSs to cell wall architecture and biogenesis, as well as their importance in cell division.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Gabriela N. Tenea
Summary: This study isolated a novel Lactococcus lactis strain from wild Amazonian camu-camu fruits, which contained various functional metabolites with antibacterial capacity. Genome analysis revealed high genetic diversity within the strain. Additionally, the strain was predicted to be non-pathogenic, lacked genes related to the production of biogenic amines and acquired antibiotic resistance genes. The study highlights the potential of this strain for producing bioactive metabolites for agri-food and pharmaceutical industry use.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Fangfang Liu, Auke J. J. van Heel, Jingqi Chen, Oscar P. P. Kuipers
Summary: Circular bacteriocins, a subgroup of ribosomally synthesized and post-translationally modified peptides, exhibit high stability and resistance to degradation, making them potential candidates for various applications. However, little is known about their biosynthesis and bioactivity. In this study, an efficient cloning and expression system for circularin A was developed, allowing for successful production and characterization of this circular peptide. Mutagenesis studies revealed the effects of specific residue mutations on bioactivity. The production conditions for bacteriocin were also optimized. Overall, this research provides valuable insights into circularin A biosynthesis and paves the way for further study.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Microbiology
Hossein Javid, Mahsa Akbari Oryani, Sanaz Akbari, Taghi Amiriani, Samaneh Ravanbakhsh, Nastaran Rezagholinejad, Amir-R Afshari, Mehdi Karimi-Shahri
Summary: Patients with intestinal inflammatory disease (IBD) have a higher risk of developing colorectal cancer (CRC). Two species of lactic acid bacteria, Lactobacillus plantarum and Lactococcus lactis, have been investigated as therapeutic agents for IBD and preventative agents for CRC.
FUTURE MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Nathalie Bechon, Jovana Mihajlovic, Anne-Aurelie Lopes, Sol Vendrell-Fernandez, Julien Deschamps, Romain Briandet, Odile Sismeiro, Isabelle Martin-Verstraete, Bruno Dupuy, Jean-Marc Ghigo
Summary: This study found that bile triggers biofilm formation in B. thetaiotaomicron and other gut Bacteroidales species. Unlike many Firmicutes and Proteobacteria that rely on eDNA for biofilm formation, B. thetaiotaomicron requires BT3563-mediated eDNA degradation to form biofilms in the presence of bile.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Marta Toth, Szabolcs Muzsai, Krzysztof Regulski, Timea Szendi-Szatmari, Zsolt Czimmerer, Eva Rajnavolgyi, Marie-Pierre Chapot-Chartier, Attila Bacsi
Summary: The human gut symbiont Lacticaseibacillus (L.) casei has a wide range of immunomodulatory effects on the human host. This study demonstrates that human monocyte-derived dendritic cells (moDCs) can engulf L. casei BL23, and the intact bacterial cell wall and morphology play a key role in this process. The absence of the bacterial cell-wall-degrading enzyme Lc-p75 leads to defective internalization and impaired cytokine secretion by moDCs, resulting in reduced T-cell activation capacity. Formation of short bacterial chains helps to exert the potent immunomodulatory properties of L. casei BL23.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Nikos Nikolopoulos, Renata C. Matos, Pascal Courtin, Isabel Ayala, Houssam Akherraz, Jean-Pierre Simorre, Marie-Pierre Chapot-Chartier, Francois Leulier, Stephanie Ravaud, Christophe Grangeasse
Summary: In this study, the cytoplasmic steps of the TA d-alanylation pathway in Lactiplantibacillus plantarum were investigated using structural biology and in vivo approaches. The protein interactions involved in this pathway were characterized, with DltC1 identified as a central hub for the cytoplasmic steps. The importance of AcpS, DltB, DltC1, and DltA in promoting Drosophila juvenile growth under chronic undernutrition was also established.
SCIENTIFIC REPORTS
(2022)
Review
Biochemistry & Molecular Biology
Hugo Guerin, Saulius Kulakauskas, Marie-Pierre Chapot-Chartier
Summary: Rha-CWPSs are crucial cell wall components of many important bacteria, playing a critical role in bacterial growth, division, and interactions with phages or hosts. This review summarizes the current knowledge on the structure, biosynthesis, and contribution of Rha-CWPSs to cell wall architecture and biogenesis, as well as their importance in cell division.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Eirini Maniki, Dimitra Kostoglou, Nikolaos Paterakis, Anastasios Nikolaou, Yiannis Kourkoutas, Alexandros Papachristoforou, Efstathios Giaouris
Summary: This study analyzed the chemical composition, antioxidant activity, and total phenolic content of the essential oil (EO) from organically cultured Thymus capitatus. The EO exhibited strong antibacterial and antibiofilm properties against three important foodborne bacterial pathogens. It could also fully destroy preformed biofilms of all three pathogens within 15 minutes. The results highlight the potential of organically cultured T. capitatus EO as a natural formulation for food and health applications.
Review
Microbiology
Raphael Charron, Marine Boulanger, Romain Briandet, Arnaud Bridier
Summary: Bacteria in biofilms on food chain surfaces show higher tolerance to biocides used during cleaning, which raises concerns about their adaptation and resistance at the single-cell and communal levels. Research has focused on understanding the specific features of biofilms that enable bacterial populations to adapt to biocide exposure. This review discusses the diversity of adaptive strategies and the potential side effects of biofilm adaptation on antimicrobial resistance, virulence, and colonization features from a One Health perspective.
Article
Biology
Nikos Nikolopoulos, Renata C. Matos, Stephanie Ravaud, Pascal Courtin, Houssam Akherraz, Simon Palussiere, Virginie Gueguen-Chaignon, Marie Salomon-Mallet, Alain Guillot, Yann Guerardel, Marie-Pierre Chapot-Chartier, Christophe Grangeasse, Francois Leulier
Summary: This study investigates the role of an uncharacterized protein (DltE) encoded by the dlt operon in Lactiplantibacillus plantarum. It is found that DltE acts as a D-Ala carboxyesterase, removing D-Ala from lipoteichoic acids (LTAs). The D-Ala-LTAs are shown to be important cues supporting intestinal peptidase expression and juvenile growth in Drosophila melanogaster.
Article
Food Science & Technology
Ioannis Geraris Kartelias, Haralabos Christos Karantonis, Efstathios Giaouris, Ioannis Panagiotakopoulos, Constantina Nasopoulou
Summary: This study successfully converted Olympus Mountain tea sweetened with honey into a novel beverage with enhanced functional properties through kombucha fermentation. The fermentation process increased the content of vitamins and antioxidant activity, and exhibited inhibitory effects on various enzymes.
Article
Food Science & Technology
Sonia Kolypetri, Dimitra Kostoglou, Anastasios Nikolaou, Yiannis Kourkoutas, Efstathios Giaouris
Summary: This study investigated the antimicrobial action of oregano essential oil (EO) against Salmonella enterica and Listeria monocytogenes. The EO exhibited strong antibacterial and antibiofilm activity, effectively eradicating biofilm cells of both pathogens. The study suggests that oregano EO has potential applications in the food and health industries.
Article
Food Science & Technology
Cedric St Martin, Nelly Caccia, Maud Darsonval, Marina Gregoire, Arthur Combeau, Gregory Jubelin, Florence Dubois-Brissonnet, Sabine Leroy, Romain Briandet, Mickael Desvaux
Summary: Functional diversity within isogenic spatially organised bacterial populations can trigger emergent community properties, such as stress tolerance. This study investigated the expression of the gadB gene, which is involved in E. coli tolerance to acidic conditions, in hydrogels mimicking structured food matrices. The spatial patterns of bacterial gene expression were visualised using confocal laser scanning microscopy. The study found that the expression patterns of gadB were radically different between neutral and acidic hydrogels. Similar spatial patterns were also observed for E. coli O157:H7 grown in the presence of L. lactis. These findings have significant implications for risk assessment and public health, highlighting the need for tailored risk mitigation strategies for different food types.
NPJ SCIENCE OF FOOD
(2023)
Article
Microbiology
Yasmine Dergham, Dominique Le Coq, Arnaud Bridier, Pilar Sanchez-Vizuete, Hadi Jbara, Julien Deschamps, Kassem Hamze, Ken-ichi Yoshida, Marie-Francoise Noirot-Gros, Romain Briandet
Summary: The NDmed strain of Bacillus subtilis has the ability to form complex biofilms and protect pathogens embedded in mixed-species biofilms. It is also genetically manipulatable, making it a valuable model for studying biofilm formation in B. subtilis.
Article
Microbiology
Yicen Lin, Romain Briandet, Akos T. Kovacs
Summary: Biofilm formation is a common process among bacterial communities, allowing them to survive. Bacillus cereus is a widely distributed species, found not only in soil but also in plant roots, nematodes, and food. Understanding the mechanisms of biofilm formation is crucial for cleaning procedures in the food industry.
Article
Microbiology
Virgile Gueneau, Julia Plateau-Gonthier, Ludovic Arnaud, Jean-Christophe Piard, Mathieu Castex, Romain Briandet
Summary: The increase in human consumption of animal proteins has led to changes in meat production management, which has resulted in stricter regulations on antimicrobial products used to control pathogens. Alternative biological solutions, such as beneficial bacteria forming positive biofilms, are being developed to improve biosecurity practices in livestock buildings. However, the mechanisms and microbial composition of these new products are still not well understood.
Article
Microbiology
Pilar Sanchez-Vizuete, Yasmine Dergham, Arnaud Bridier, Julien Deschamps, Etienne Dervyn, Kassem Hamze, Stephane Aymerich, Dominique Le Coq, Romain Briandet
Summary: Bacillus subtilis can form biphasic biofilms on submerged surfaces, where bacteria initially adhere to the surface forming elongated chains before being released as free cells. The switching coincides with oxygen depletion and is followed by the formation of the biofilm at the liquid-air interface. Residual bacteria associated with the solid surface express matrix genes to build protruding structures typical of biofilms.
Review
Medicine, Research & Experimental
Celia Sahli, Sergio E. Moya, John S. Lomas, Christine Gravier-Pelletier, Romain Briandet, Miryana Hemadi
Summary: This article introduces the spatially-organized communities of microorganisms called biofilms and their interactions with antimicrobials. The limitations of antibiotic treatments are discussed, and alternative approaches using nanoparticles are presented. The aim of this article is to eradicate bacterial biofilms and fight antimicrobial tolerance using nanobiotechnology-based approaches.
