Article
Chemistry, Medicinal
Harsha Gopal Agrawal, Pravin Shankar Giri, Poonam Meena, Subha Narayan Rath, Ashutosh Kumar Mishra
Summary: A bioinspired design based on a neutral flavin-triphenylamine core has been investigated for its selective mitochondrial bioimaging capabilities in different microenvironments. The design showed significant advantages such as long-term tracking, faster internalization, penetrability within spheroid structures, and strong emission signal under induced hypoxia conditions, offering an alternative for studying hypoxia-related biological events.
ACS MEDICINAL CHEMISTRY LETTERS
(2023)
Review
Oncology
Sylvain Garciaz, Colombe Saillard, Yosr Hicheri, Marie-Anne Hospital, Norbert Vey
Summary: Venetoclax, a BH3-mimetics agent targeting the antiapoptotic protein BCL-2, has revolutionized the treatment landscape for AML patients unfit for intensive chemotherapy. The drug has shown promising overall response rates and survival outcomes in combination with various therapies, including hypomethylating agents and high-dose chemotherapy, but resistance to venetoclax remains a challenge that requires further investigation and development of novel therapeutic strategies.
Article
Spectroscopy
Xiao-Lei Xue, Hao Zhang, Gui-Hua Chen, Guan-Hua Yu, Hao-Ran Hu, Shu-Yan Niu, Kun- Peng Wang, Zhi-Qiang Hu
Summary: There is a pressing need for the development of highly sensitive and selective fluorescence probes for ONOO- in mitochondria. In this study, we introduced a ratiometric fluorescent probe COUS, which utilized a coumarin-cyanine hybrid as the fluorophore and C = C bonds as reaction sites of ONOO-. The probe COUS displayed sensitivity and selectivity towards ONOO-, with a large fluorescence emission shift (239 nm) and a low detection limit (41.88 nM). Furthermore, COUS demonstrated mitochondrial targeting ability, and the targeting moiety could dissociate from the probe upon reaction with ONOO-, enabling accurate detection of ONOO- in mitochondria.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Analytical
Jianbin Chao, Jiamin Zhao, Yongbin Zhang, Fangjun Huo, Caixia Yin
Summary: Biothiols play important physiological and pathological roles in organisms, but their specific markers are challenging to identify due to their high reactivity and short half-life. By designing reaction sites and studying intramolecular rearrangement, different biothiols can be distinguished successfully.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Applied
Liren Xu, Jin Yu, Yunpeng Wang, Yuru Chen, Xiaoli Zhang, Rubing Han, Jing Jing, Rubo Zhang, Xiaoling Zhang
Summary: In this study, a fluorescent probe PCH was synthesized to detect ClO- in mitochondria. PCH can target mitochondria with fast response speed, high selectivity, large Stokes shift, and low detection limitation. With this probe, mitochondrial ClO- can be detected endogenously and exogenously.
Review
Biochemistry & Molecular Biology
Walter Douglas Fairlie, Erinna F. Lee
Summary: BCL-2, c-MYC and related proteins play crucial roles in normal cell functioning and are central to various cancers. Genetic lesions leading to deregulation of these proteins contribute to tumor development and survival depending on the cooperation between these protein families.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Spectroscopy
Hai Wu, Qinqin Xu, Kun Yin, Zhaoqiang Liu, Tian Xie, Li Wang, Yuanyuan Li, Mengjie Zhang, Xiaojun Lv, Wenyong Li, Suhua Fan
Summary: A novel fluorescent probe with turn-on fluorescence response, high sensitivity, good compatibility, and mitochondria-targeting function has been synthesized for detecting and visualizing cyanide. The probe utilizes an electron-donating triphenylamine group as the fluorescent, an electron-accepting moiety as the mitochondria-targeted localization unit, and an intramolecular charge transfer system. The probe showed a turn-on fluorescence response towards cyanide due to the insertion of an electron-deficient group and the inhibition of intramolecular charge transfer induced by the nucleophilic addition of cyanide. The probe demonstrated successful detection of cyanide in food samples and showed low cytotoxicity, clear mitochondria-localizing capability, and excellent fluorescence imaging in living cells.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Review
Chemistry, Multidisciplinary
Hannah Crawford, Maria Dimitriadi, Jatinder Bassin, Michael T. Cook, Thais Fedatto Abelha, Jesus Calvo-Castro
Summary: In the past decade, there has been an increasing number of studies focusing on the development of novel small organic conjugated systems for mitochondrial imaging using optical signal transduction pathways. Mitochondria play a critical role in key biological processes, such as cellular metabolism. Consequently, targeting mitochondria for therapeutic benefits has become increasingly important. This work provides an in-depth evaluation of progress in this field, paving the way for superior alternatives to current methods.
CHEMISTRY-A EUROPEAN JOURNAL
(2022)
Article
Chemistry, Analytical
Wei Shu, Jin Yu, Huidan Wang, Anji Yu, Liyan Xiao, Zhuohang Li, Haitao Zhang, Yu Zhang, Yulong Wu
Summary: In this study, a reversible fluorescent probe GeP was designed for detecting intracellular glutathione. The probe exhibited fast response and high fluorescence change, and could target mitochondria. Additionally, GeP enabled superresolution fluorescence imaging of mitochondria.
ANALYTICA CHIMICA ACTA
(2022)
Article
Environmental Sciences
Liuliang Yuan, Wen-Xiong Wang
Summary: In this study, the subcellular fate and behavior of Zn and Cu in zebrafish cells were investigated. The results showed completely different behaviors and distributions of Zn and Cu, with implications for understanding their toxicity and cellular functions.
ENVIRONMENTAL POLLUTION
(2022)
Article
Chemistry, Analytical
Qi Wang, Yanzhe Zhu, Debao Chen, Jiale Ou, Man Chen, Yan Feng, Wenbin Wang, Xiangming Meng
Summary: A fluorescent probe Mito-FCC was designed for efficient and selective detection of NADH, and successfully visualized tumors in vivo. This probe holds great potential in studying mitochondrial function and diagnosing cancer.
Article
Biochemistry & Molecular Biology
Medha, Priyanka, Parul Bhatt, Sadhna Sharma, Monika Sharma
Summary: This study investigates the role of the unique C-terminal of Mycobacterium tuberculosis's (Mtb) PE6/Rv0335c protein in causing host mitochondrial perturbations and apoptosis. The experiments confirm that the deletion of the C-terminal domain significantly reduces mitochondrial perturbations and apoptosis, while not affecting the protein's localization to mitochondria. Additionally, the study shows that the protein increases intracellular calcium influx, with the C-terminal deletion having minimal effect on calcium influx. These findings suggest that Rv0335c may mimic eukaryotic Bcl2 proteins, leading to host mitochondrial perturbations and apoptosis.
Article
Chemistry, Applied
Xin Ji, Nannan Wang, Jian Zhang, Shuang Xu, Yubing Si, Weili Zhao
Summary: In this study, researchers designed and synthesized a novel fluorescent probe for rapid and accurate detection of cysteine (Cys) concentration in biological systems, particularly in mitochondria. The probe exhibited a low detection limit and successfully enabled monitoring of Cys in living cells and in vivo.
Article
Biochemistry & Molecular Biology
Sun Hyeok Lee, Chul Soon Park, Kyung Kwan Lee, Tae-Hee Han, Hyun Seung Ban, Chang-Soo Lee
Summary: In this study, novel near-infrared probes were synthesized for fluorescence imaging of NTR activity. NIR-HCy-NO2 1 exhibited superior performance compared to the other two probes in terms of catalytic efficiency and limit of detection. Furthermore, NIR-HCy-NO2 1 was found to react with type I mitochondrial NTR in live cell imaging. NIR-HCy-NO2 demonstrated great potential for various NTR-related applications, including NTR activity imaging in vivo.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Sukhvinder Dhiman, Rasdeep Kour, Satwinderjeet Kaur, Prabhpreet Singh, Subodh Kumar
Summary: A new fluorescent probe DMAS-Si was reported in this study for the simultaneous detection of mitochondrial viscosity and F- ion through different signals, showing significant potential for investigating the physiological state of mitochondria.
BIOORGANIC CHEMISTRY
(2022)