Article
Microbiology
Arezoo Mirzaei, Jeroen Wagemans, Bahram Nasr Esfahani, Rob Lavigne, Sharareh Moghim
Summary: The rapid spread of MDR and XDR bacterial strains and biofilm formation pose challenges in hospitals. This study focuses on isolating and characterizing two lytic phages to control biofilms caused by P. mirabilis infections. The phage cocktail significantly reduced biofilm formation and downregulated genes involved in biofilm formation.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Jonathan T. Butement, Daniel J. Noel, Catherine A. Bryant, Sandra A. Wilks, Robert W. Eason
Summary: Catheter-associated urinary tract infection (CAUTI) is a major cause of hospital-acquired infections worldwide, leading to significant burdens. This study presents a novel concept of a light-guiding urinary catheter that uses antimicrobial blue light to prevent biofilm formation. The results show that this concept is highly effective in inhibiting biofilm formation.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Urology & Nephrology
Fei Yuan, Ziye Huang, Tongxin Yang, Guang Wang, Pei Li, Bowei Yang, Jiongming Li
Summary: Proteus mirabilis, a Gram-negative rod-shaped bacterium, is widely present in the natural environment and is most noted for its swarming motility and urease activity. It is the main pathogen causing complicated UTIs, especially catheter-associated UTIs, and forms crystalline biofilms on catheters. The bacterium can lead to various urinary tract infections and fatal complications through different virulence factors.
UROLOGIA INTERNATIONALIS
(2021)
Article
Microbiology
Roman Herout, Sara Khoddami, Igor Moskalev, Alina Reicherz, Ben H. Chew, Chelsie E. Armbruster, Dirk Lange
Summary: This study investigated the role of bacterial surface components (BSCs) in the pathogenicity of Proteus mirabilis, a bacterium that causes catheter-associated urinary tract infections. The results showed that BSCs play a critical role in the adhesion of the bacteria to medical devices and invasion of urinary tissue.
Article
Immunology
Grzegorz Czerwonka, Dawid Gmiter, Katarzyna Durlik-Popinska
Summary: Proteus mirabilis is a pathogenic bacterium causing urinary tract infections, with factors like swarming motility, urease production, and LPS contributing to its virulence. The O18 serogroup of this bacterium decorates LPS with ChoP, which plays a significant role in pathogenicity by affecting bacterial adhesion, resistance to antimicrobial peptides, and sensitivity to complement-mediated killing. The genome of P. mirabilis O18 contains genes associated with choline phosphorylation, uptake, and transfer, with homologs related to LicC from Streptococcus pneumoniae.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Cormac J. Rice, Stephen A. Kelly, Seamus C. O'Brien, Erinn M. Melaugh, Jan C. B. Ganacias, Zheng Hua Chai, Brendan F. Gilmore, Timofey Skvortsov
Summary: Proteus mirabilis adheres to urinary catheters and forms biofilms, leading to blockage and decreased antibiotic effectiveness, potentially causing antibiotic resistance. Bacteriophages and their enzymes are being explored as alternative treatment options.
Article
Multidisciplinary Sciences
James E. Corban, Jolene Ramsey
Summary: Characterization of the Proteus mirabilis bacteriophage Privateer isolated from Texas wastewater treatment plant activated sludge revealed genetic features and evolutionary relationships of structural proteins with other prolate podophages.
Article
Biochemistry & Molecular Biology
Aimee L. Brauer, Brian S. Learman, Steven M. Taddei, Namrata Deka, Benjamin C. Hunt, Chelsie E. Armbruster
Summary: This study demonstrates that P. mirabilis prefers to catabolize l-serine in human urine, followed by d-serine, threonine, tyrosine, glutamine, tryptophan, and phenylalanine. Disruption of serine catabolism has minimal impact on in vitro phenotypes, but disrupting both pathways leads to decreased motility, biofilm formation, and fitness.
MOLECULAR MICROBIOLOGY
(2022)
Article
Microbiology
Melanie M. Pearson, Sapna Pahil, Valerie S. Forsyth, Allyson E. Shea, Harry L. T. Mobley
Summary: Ordered transposon libraries are a valuable resource for studying bacterial species, especially those with difficult methods for targeted genetic mutations. In this study, we constructed an ordered transposon library for the bacterial urinary tract pathogen Proteus mirabilis strain HI4320. The library enables large-scale screens and facilitates the study of P. mirabilis biology. However, the library's coverage of predicted genes was lower than expected due to unmapped insertions and a high proportion of mixed clones and multiple transposon insertion events. Future library construction can be improved by addressing these challenges.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Yi Huang, Jinbin Chen, Qiao Jiang, Nan Huang, Xin Ding, Liang Peng, Xiaoyan Deng
Summary: This study revealed the indirect role of ModA in the anaerobic growth, motility, biofilm formation, and pathogenicity of P. mirabilis, emphasizing the importance of ModA in mediating molybdate uptake and allowing the bacterium to adapt to complex environmental conditions and cause UTIs.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Immunology
Dawid Gmiter, Wieslaw Kaca
Summary: Indwelling urinary catheterization can lead to catheter-associated urinary tract infections (CAUTIs), with Proteus mirabilis identified as an important cause. The pathogenicity of P. mirabilis is linked to adherence and swarming motility, which are important for biofilm formation and migration. These processes involve multiple interactions and contribute to the virulence.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Medicine, General & Internal
Hae Do Jung, Seok Cho, Joo Yong Lee
Summary: Microbiota plays a role in kidney stone formation through various mechanisms, including hyperoxaluria, calcium oxalate supersaturation, biofilm formation, and urothelial injury. The urinary tract microbiome differs between cohorts with and without urinary stone disease, and certain bacteria are associated with stone formation. Standardization is essential in urinary microbiome research for urolithiasis.
Article
Microbiology
O. E. Clarke, H. Pelling, V. Bennett, T. Matsumoto, G. E. Gregory, J. Nzakizwanayo, A. J. Slate, A. Preston, M. Laabei, L. J. Bock, M. E. Wand, K. Ikebukuro, S. Gebhard, J. M. Sutton, B. V. Jones
Summary: Chlorhexidine (CHD), a commonly used biocide in healthcare settings, is often considered ineffective against Proteus mirabilis in catheter infection control. This study investigated the mechanisms of reduced CHD susceptibility in P. mirabilis and found that a mutation in the waaC gene, involved in lipopolysaccharide (LPS) biosynthesis, resulted in decreased CHD minimum inhibitory concentrations (MICs). The mutation also increased cell surface hydrophobicity and susceptibility to other cationic biocides. Additionally, repression of the smvA efflux system further enhanced CHD susceptibility. These findings highlight the importance of LPS structure and the smvA efflux system in modulating susceptibility to CHD and other biocides in P. mirabilis.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Paulina Stolarek, Przemyslaw Bernat, Antoni Rozalski
Summary: This study confirmed the effectiveness of amikacin or gentamicin and ascorbic acid co-therapy in eliminating uropathogenic cells and found that ascorbic acid can be an effective urine acidifier for preventing catheter-associated urinary tract infections (CAUTIs).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Immunology
Agata Palusiak
Summary: The genera Klebsiella and Proteus, both Gram-negative rods, colonize the human intestinal tract and are considered the main reservoir of opportunistic pathogens. These pathogens, particularly K. pneumoniae and P. mirabilis, are the leading causes of infections in the urinary and respiratory tracts, wounds, and bacteremia, commonly affecting immunocompromised patients. The similarities in the structure of urease, lipids A, and the core regions of lipopolysaccharides produced by these pathogens highlight the potential for finding cross-reacting vaccine antigens. Understanding the structural and serological similarities between Klebsiella spp. and Proteus spp. polysaccharides is crucial in the search for effective vaccine antigens.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Antoni W. Rozalski
Article
Biochemical Research Methods
Yuhui Du, Huiying Li, Zhiqiu Yin, Antoni Rozalski, Agnieszka Torzewska, Pan Yang, Chengqian Qian, Tingting Xu, Hengchun Cao, Pan Wu, Lingyan Jiang, Xi Guo, Di Huang, Bin Liu
JOURNAL OF MICROBIOLOGICAL METHODS
(2018)
Article
Crystallography
Jolanta Prywer, Agnieszka Torzewska
JOURNAL OF CRYSTAL GROWTH
(2019)
Article
Microbiology
Agnieszka Torzewska, Katarzyna Bednarska, Antoni Rozalski
RESEARCH IN MICROBIOLOGY
(2019)
Article
Multidisciplinary Sciences
Jolanta Prywer, Agnieszka Torzewska
SCIENTIFIC REPORTS
(2019)
Correction
Multidisciplinary Sciences
Jolanta Prywer, Agnieszka Torzewska
SCIENTIFIC REPORTS
(2020)
Article
Biotechnology & Applied Microbiology
Agnieszka Torzewska, Paulina Wiewiura, Dominika Brodecka, Dominika Szczerbiec, Antoni Rozalski
Summary: Proteus mirabilis is a common microorganism causing infectious urolithiasis, while lactobacilli strains have antibacterial properties that can influence urinary crystallization.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2021)
Article
Multidisciplinary Sciences
Agnieszka Maszewska, Magdalena Moryl, Junli Wu, Bin Liu, Lu Feng, Antoni Rozalski
Summary: The modification of outer membrane proteins (OMPs) is crucial for Gram-negative bacteria defense against antimicrobials. Proteus mirabilis OMPs play a significant role in antibiotic and phage resistance, with upregulated and downregulated proteins identified in resistant variants. The use of phages in combination with antibiotics can reduce the number of differentially expressed proteins, suggesting potential therapeutic implications for P. mirabilis antibiofilm therapies.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Paulina Stolarek, Przemyslaw Bernat, Dominika Szczerbiec, Antoni Rozalski
Summary: This study investigated the changes in lipidome components during P. mirabilis adhesion to urinary catheters, finding certain phospholipids and fatty acids associated with adhesion and hydrophobicity. Depolarization of the inner membrane and high surface hydrophobicity were identified as key factors for bacterial adhesion to silicone catheters, with adherent cells having lower membrane packing density compared to planktonic cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Dominika Szczerbiec, Justyna Piechocka, Rafal Glowacki, Agnieszka Torzewska
Summary: This study investigates the antibacterial effect of organic acids secreted by Lactobacillus strains on urinary tract infections caused by P. mirabilis. The results show that these acids have a high inhibitory effect on the growth and biofilm formation of P. mirabilis and could be used as novel agents for the treatment of urinary tract infections.
Article
Multidisciplinary Sciences
Mikolaj Skubisz, Agnieszka Torzewska, Ewa Mielniczek-Brzoska, Jolanta Prywer
Summary: The presence of phosphoric acid in carbonated drinks has an effect on the formation and growth of urinary stones. It causes the nucleation of struvite crystals at a lower pH, resulting in a greater amount of struvite formed. The concentration of phosphoric acid also affects the size of the struvite crystals, with higher concentrations leading to larger crystals that are more difficult to remove from the urinary tract.
SCIENTIFIC REPORTS
(2022)
Article
Medicine, General & Internal
Magdalena Moryl, Aleksandra Palatynska-Ulatowska, Agnieszka Maszewska, Iwona Grzejdziak, Silvia Dias de Oliveira, Marieli Chitolina Pradebon, Liviu Steier, Antoni Rozalski, Jose Antonio Poli de Figueiredo
Summary: This study isolated and characterized two phages against E. faecalis and confirmed their lytic activity against the bacteria. The phages showed efficacy against E. faecalis biofilm in an ex vivo model, supporting the potential of phage therapy for prevention and treatment of E. faecalis-associated infections.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Malgorzata Siwinska, Agnieszka Zablotni, Evgeniya A. Levina, Alexander S. Shashkov, Olga G. Ovchinnikova, Antoni Rozalski, Yuriy A. Knirel
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2020)
