Journal
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
Volume 107, Issue 1, Pages 9-14Publisher
SPRINGER
DOI: 10.1007/s10482-014-0298-x
Keywords
Sphaerisporangium dianthi sp nov.; Polyphasic taxonomy; 16S rRNA gene
Categories
Funding
- National Outstanding Youth Foundation [31225024]
- National Key Project for Basic Research [2010CB126102]
- National Key Technology RD Program [2012BAD19B06]
- Program for New Century Excellent Talents in University [NCET-11-0953]
- National Natural Science Foundation of China [31372006, 31171913, 31071750, 31471832]
- Outstanding Youth Foundation of Heilongjiang Province [JC201201]
- Chang Jiang Scholar Candidates Program for Provincial Universities in Heilongjiang (CSCP)
- Science and Technology Research Project of Heilongjiang Provincial Educational Commission [12541001]
- Youth Science Foundation of Heilongjiang Province [QC2014C013]
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A novel actinomycete, designated strain NEAU-CY18(T), was isolated from the root of a Chinese medicinal plant Dianthus chinensis L and subjected to a polyphasic taxonomic study. The novel strain was found to develop spherical sporangia with non-motile spores on aerial mycelium. The cell-wall peptidoglycan was found to contain meso-diaminopimelic acid. The whole-cell sugars were identified as madurose, mannose, ribose, galactose and glucose. The phospholipid profile was found to contain diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxy-phosphatidylmethylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides and an unidentified phospholipid. The predominant menaquinones were identified as MK-9(H-4), MK-9(H-2) and MK-9(H-6). The major fatty acids were identified as C-17:0 10-methyl, iso-C-16:0 and C-16:0. EzTaxon-e analysis of the 16S rRNA gene sequence indicated that the strain belongs to the genus Sphaerisporangium and was most closely related to Sphaerisporangium cinnabarinum JCM 3291(T) (98.9 %) and Sphaerisporangium melleum JCM 13064(T) (98.3 %). Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain NEAU-CY18(T) forms a monophyletic clade with S. cinnabarinum JCM 3291(T), an association that was supported by a bootstrap value of 97 % in the neighbour-joining tree and also recovered with the maximum-likelihood algorithm. Comparisons of some phenotypic properties and low DNA-DNA relatedness values enabled the strain to be differentiated from S. cinnabarinum JCM 3291(T) and S. melleum JCM 13064(T). Therefore, it is concluded that strain NEAU-CY18(T) represents a novel Sphaerisporangium species, for which the name Sphaerisporangium dianthi sp. nov. is proposed. The type strain is NEAU-CY18(T) ( = CGMCC 4.7132(T) = DSM 46736(T)).
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