Nocardioides soli sp nov., a bacterium isolated from a mountain soil

Strain SR-1(T), a Gram-positive, strictly-aerobic, short-rod shaped, non-motile bacterium, was isolated from a mountain soil collected in Seoul Women's University in South Korea. Growth occurred between 15 and 37 A degrees C (optimum, 30 A degrees C), at pH 6.0-9.0 (optimum, pH 7.0) and with 0-2 % NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SR-1(T) belongs to the genus Nocardioides and is closely related to Nocardioides simplex KCTC 9106(T) (96.8 %), Nocardioides caeni MN8(T) (96.7 %), Nocardioides aromaticivorans H-1(T) (96.6 %), and Nocardioides kongijuensis A2-4(T) (96.6 %). Chemotaxonomic data revealed that strain SR-1(T) possesses MK-8(H-4) as predominant menaquinone, ll-2,6-diaminopimelic acid as the diagnostic diamino acid, phosphatidylglycerol and diphosphatidylglycerol as predominant polar lipids and iso-C-16:0,C- 10-methyl-C-18:0, and C-18:1 omega 9c are major fatty acids. The DNA G+C content of the strain SR-1(T) was 72.4 mol%. Based on polyphasic evidence, strain SR-1(T) (= KEMC 9004-134(T) = JCM 19684(T)) should be classified as the type strain of a novel Nocardioides species, for which the name Nocardioides soli sp. nov. is proposed.