Article
Microbiology
Qianhui Zhu, Jinrui Hu, Na Liu, Heyuan Qi, Xiaoli Du, Zhigang Cui, Yan Sun, Yadong Liu, Songnian Hu, Linhuan Wu, Haijian Zhou, Zilong He, Juncai Ma
Summary: Antibiotic resistance is a global health and biosafety problem. Previously undescribed Cronobacter isolates carrying mcr genes, mainly mcr-9 and mcr-10, have been identified. These findings suggest that foodborne Cronobacter isolates carrying mcr genes pose a threat to human health.
Article
Microbiology
Zeinab Ebrahimzadeh Mousavi, Leonard Koolman, Guerrino Macori, Seamus Fanning, Francis Butler
Summary: This study characterized Cronobacter sakazakii strains through whole-genome sequencing and identified important features, such as virulence genes and multiple antibiotic resistance. Furthermore, the study compared the strains globally, contributing to a better understanding of Cronobacter outbreaks and the development of prevention and control strategies.
Article
Food Science & Technology
Haiyan Zeng, Chengsi Li, Dandan Luo, Jumei Zhang, Yu Ding, Moutong Chen, Xiaojuan Yang, Tao Lei, Shi Wu, Qinghua Ye, Rui Pang, Qihui Gu, Qingping Wu
Summary: A novel lytic phage B1 was isolated from river water in Guangzhou, China, which can simultaneously lyse C. malonaticus and C. turicensis, showing potential for controlling foodborne pathogens. The phage has high stability, strong lytic ability, and can significantly inhibit the growth of Cronobacter spp., especially C. turicensis, making it a promising candidate for food safety.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Food Science & Technology
Dandan Luo, Chengsi Li, Qingping Wu, Yu Ding, Meiyan Yang, Yongdan Hu, Haiyan Zeng, Jumei Zhang
Summary: Cronobacter spp. phage vB_CtuP_A24, a novel lytic phage with the ability to lyse five Cronobacter species, shows stability under various environmental conditions and potential as a promising biocontrol agent against Cronobacter spp. in food production.
FOOD RESEARCH INTERNATIONAL
(2021)
Review
Engineering, Multidisciplinary
Na Ling, Xiuting Jiang, Stephen Forsythe, Danfeng Zhang, Yizhong Shen, Yu Ding, Juan Wang, Jumei Zhang, Qingping Wu, Yingwang Ye
Summary: This review summarizes the prevalence of Cronobacter species in various food types and highlights its high virulence in infants. The mechanisms allowing Cronobacter to persist in adverse growth conditions and its pathogenicity are discussed. The food safety concerns caused by Cronobacter and the subsequent control methods and clinical treatments are emphasized.
Article
Infectious Diseases
Cui-Hong An, Shou-Min Nie, Yang-Xin Sun, Suo-Ping Fan, Bo-Yan Luo, Zhenjun Li, Zhi-Guo Liu, Wen-Hui Chang
Summary: Analysis of blood samples from populations suspected of Brucella spp. infections revealed significant differences in seroprevalence over time, in different areas, and different cities, indicating sporadic cases as a dominant epidemic characteristic with occasional outbreaks. Strains from northern provinces were identified as a potential source of human brucellosis cases in Shaanxi Province, highlighting the importance of enhancing surveillance and control measures for the trade and transfer of infected sheep among regions.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Plant Sciences
Dali Gaganidze, Tinatin Sadunishvili, Mariam Aznarashvili, Ekaterine Abashidze, Manana Gurielidze, Simon Carnal, Fabio Rezzonico, Manana Zubadalashvili
Summary: Since its initial appearance in the Mtskheta-Mtianeti region in 2016, fire blight has spread to several other regions of Georgia during the years 2017-2018, especially affecting the eastern part of the country. The causative agent, Gram-negative bacterium Erwinia amylovora, was confirmed by PCR in hundreds of instances on different organs of symptomatic apple, quince, and pear trees. Sequencing of the CRISPR regions revealed the presence of two distinct genotypes that can be traced back to Europe in the twentieth century, indicating multiple introduction events through import of diseased plant material in recent years.
JOURNAL OF PLANT PATHOLOGY
(2021)
Article
Food Science & Technology
Irati Martinez-Malaxetxebarria, Cecilia Girbau, Adrian Salazar-Sanchez, Itsaso Baztarrika, Ilargi Martinez-Ballesteros, Lorena Laorden, Rodrigo Alonso, Aurora Fernandez-Astorga
Summary: The study found various species of Arcobacter in different food samples, with most identified as A. butzleri and A. cryaerophilus. These strains were mainly derived from foods such as squids and turkey, with squids showing the highest contamination rate. Evaluation of biofilm activity revealed the survival and growth ability of Arcobacter species on different food contact surfaces.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Food Science & Technology
Monika Garbowska, Anna Berthold-Pluta, Lidia Stasiak-Rozanska, Antoni Pluta, Stephen Forsythe, Ilona Stefanska
Summary: This study investigated the genotyping diversity and hemolytic properties of Cronobacter spp. strains isolated from commercial ready-to-eat plant-based foods. The results showed high genotypic diversity and pathogenicity of these strains. Several new sequence types and alleles were also discovered.
Article
Food Science & Technology
Ondrej Holy, Julio Parra-Flores, Sarah Lepuschitz, Maria Paula Alarcon-Lavin, Ariadnna Cruz-Cordova, Juan Xicohtencatl-Cortes, Jetsi Mancilla-Rojano, Werner Ruppitsch, Stephen Forsythe
Summary: This study investigated the putative virulence factors of six Cronobacter sakazakii strains isolated from powdered milk, identifying different sequence types and resistance genes. Some strains demonstrated high adherence and invasion abilities, while the cpa gene was not detected. Various virulence and resistance genes, as well as plasmids, were identified through whole-genome sequencing. Additionally, CRISPR matrices were observed in all strains, enhancing decision-making for health authorities to protect public health.
Article
Plant Sciences
Marco Dorfner, Tankred Ott, Philipp Ott, Christoph Oberprieler
Summary: SLANG is a bioinformatic pipeline for locus assembly, orthology estimation, and SNP calling using Nanopore-sequenced multi-locus data. Combined with an AFLP-based library preparation, SLANG provides a cost-effective and affordable alternative.
APPLICATIONS IN PLANT SCIENCES
(2022)
Article
Food Science & Technology
Ling Wang, Li Wang, Youzhao Liu, Zhiyun Wang, Qing Chen, Zhihua Liu, Jing Hu
Summary: The study identified two CRISPR systems in Laribacter hongkongensis, with the CRISPR1-Cas system being present in the majority of isolates. It also highlighted the potential for molecular typing of L. hongkongensis based on the conservation of CRISPR loci and variable repeat-spacer arrays.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2021)
Article
Food Science & Technology
Lu Wang, Wenxuan Zhu, Gege Lu, Pan Wu, Yi Wei, Yingying Su, Tianyuan Jia, Linxing Li, Xi Guo, Min Huang, Qian Yang, Di Huang, Bin Liu
Summary: Traditional serotyping methods for foodborne pathogen Cronobacter are time-consuming and inefficient, but the newly developed CroTrait tool enables rapid and accurate species identification and O serotyping based on whole-genome sequencing data. Additionally, it has the capability to predict new potential O serotypes, making it a convenient and promising tool for Cronobacter isolates.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2021)
Review
Food Science & Technology
Carine da Fonseca Cechin, Gabriela Guimaraes Carvalho, Caroline Peixoto Bastos, Dirce Yorika Kabuki
Summary: This review article discusses the occurrence and prevalence of Cronobacter spp. in plant-based foods, as well as the possible sources and routes of contamination, and the presence of pathogenic strains. The study found that Cronobacter can be found in a variety of plant-based foods, cultivation environments, and food processing industries. It also identified sequence types associated with clinical cases and antibiotic-resistant isolates.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Food Science & Technology
Simone de Fatima Rauber Wurfel, Denise da Fontoura Prates, Natalie Rauber Kleinubing, Joline Dalla Vecchia, Cristiane Vaniel, Louise Haubert, Odir Antonio Dellagostin, Wladimir Padilha da Silva
Summary: The study found high antimicrobial resistance and genetic diversity in Campylobacter isolates from broiler meat products in southern Brazil. Multidrug-resistant Campylobacter, especially in C. jejuni, was prevalent. Data analysis revealed that antimicrobial resistance and virulence profiles were not species-specific.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Biology
Alexandra Simonovicova, Daniel Kupka, Sanja Nosalj, Lucia Krakova, Hana Drahovska, Zuzana Bartova, Hana Vojtkova, Katerina Boturova, Domenico Pangallo
Article
Multidisciplinary Sciences
Katarina Soltys, Martina Stuchlikova, Tibor Hlavaty, Barbora Gaalova, Jaroslav Budis, Juraj Gazdarica, Anna Krajcovicova, Zuzana Zelinkova, Tomas Szemes, Daniel Kuba, Hana Drahovska, Jan Turna, Stanislav Stuchlik
SCIENTIFIC REPORTS
(2020)
Article
Virology
Aneta Lichvarikova, Katarina Soltys, Tomas Szemes, Livia Slobodnikova, Gabriela Bukovska, Jan Turna, Hana Drahovska
Article
Biochemical Research Methods
Barbora Markuskova, Jana Minarovicova, Adriana Veghova, Hana Drahovska, Eva Kaclikova
Summary: This study evaluated the impact of different DNA extraction methods on the analysis of bacterial communities in cheese and found that various extraction procedures can lead to variable results, with the lysis method affecting the abundance profiles significantly. It also highlighted the risk of comparing bacterial community data in cheese when using different DNA extraction protocols and emphasized the need for a standardized approach for comparison across multiple sequencing runs.
JOURNAL OF MICROBIOLOGICAL METHODS
(2021)
Article
Virology
Livia Slobodnikova, Barbora Markuskova, Michal Kajsik, Michal Andrezal, Marek Straka, Adriana Liptakova, Hana Drahovska
Summary: The study isolated and characterized two bacteriophages with broad host specificity against local uropathogenic Escherichia coli strains, successfully combining them into a phage cocktail with good activity against polyresistant strains. This research represents a preliminary step towards establishing a national phage bank in Slovakia with well-characterized phages for therapeutic potential.
Article
Microbiology
Alexandra Simonovicova, Hana Vojtkova, Sanja Nosalj, Elena Pieckova, Hana Svehlakova, Lucia Krakova, Hana Drahovska, Barbara Stalmachova, Katerina Kucova, Domenico Pangallo
Summary: This study presents a biological profile of 16 Aspergillus niger isolates from various environments, revealing high levels of toxic elements in contaminated soils and solid substrates. Molecular PCR and biochemical tests were used to confirm identification and characteristics of the isolates, with some strains capable of synthesizing mycotoxins. Environmental contamination may lead to genetic alterations in fungal species and influence their physiology, potentially resulting in the production of harmful metabolites.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biology
Maria Kajsikova, Michal Kajsik, Hana Drahovska, Gabriela Bukovska
Summary: In this study, we analyzed the genome of [B.] flavum CCM 251 and identified prophage-related sequence regions. In silico analysis of the genome revealed two complete aspartate biosynthetic pathways involved in L-lysine production.
Article
Biotechnology & Applied Microbiology
Lucia Bocanova, Michal Psenko, Imrich Barak, Nora Halgasova, Hana Drahovska, Gabriela Bukovska
Summary: The endolysin EN534 has the potential to become an antimicrobial agent for treating Streptococcus agalactiae infections, showing good lytic activity and stability.
JOURNAL OF BIOTECHNOLOGY
(2022)
Article
Infectious Diseases
Jan Koren, Michal Andrezal, Hana Drahovska, Zuzana Hubenakova, Adriana Liptakova, Tibor Maliar
Summary: Carbapenem-resistant Klebsiella pneumoniae is a serious threat in university hospitals, with different sequence types associated with intra-hospital and inter-hospital transmission. These strains exhibit high levels of antibiotic resistance. Preventing the spread of these pathogens between healthcare facilities is crucial.
Article
Virology
Michal Andrezal, Lucia Oravcova, Veronika Kadlicekova, Elham Ozaee, Sulafa Elnwrani, Juraj Bugala, Barbora Markuskova, Michal Kajsik, Hana Drahovska
Summary: In this study, four closely related bacteriophages belonging to Tevenvirinae subfamily were isolated, which infect Cronobacter and Enterobacter strains. Among them, bacteriophage Pet-CM3-4 exhibited broader host specificity towards Enterobacter hosts. Based on genome sequences, all these phages were assigned to the genus Karamvirus. Factors influencing the host specificity of Pet-CM3-4 phage and its host range mutant Pet-CM3-1 were studied, and a substitution in the long tail fiber adhesin was found to be responsible for the reduced host specificity of Pet-CM3-1. Furthermore, LPS was identified as the receptor for both phages, with C. malonaticus O:3 antigen being the receptor for Pet-CM3-1 and structures of the LPS core region being the receptor for Pet-CM3-4. These findings contribute to our understanding of the biology and evolution of Tevenvirinae phages.
Article
Microbiology
Michal Kajsik, Peter Durovka, Maria Kajsikova, Diana Rusnakova, Tomas Szemes, Hana Drahovska
Summary: This report describes the characterization of Cronobacter phage Dev_CS701, a ubiquitous Gram-negative bacterium linked with serious infections, isolated from wastewater. Dev_CS701, related to phages belonging to the Straboviridae family and Pseudotevenvirus genus, contains 257 predicted protein-coding genes and a tRNA gene.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Article
Food Science & Technology
Zuzana Reskova, Michal Andrezal, Hana Drahovska, Janka Korenova, Janka Lopasovska, Tomas Kuchta
Summary: Microbial contamination of oil seeds can lead to deterioration and reduction of oil quality. Studying the levels and composition of microbial communities can help establish effective control measures. The bacterial community in hemp seeds is mainly composed of Enterobacteriaceae and Pseudomonadaceae, while the fungal community is mainly composed of Pleosporaceae, Davidiellaceae, and Tremellaceae. In poppy seeds, the bacterial community is mainly composed of Enterobacteriaceae (genus Klebsiella), Pseudomonadaceae, and Xanthomonadaceae or Streptomycetaceae. The fungal community is mainly composed of Pleosporaceae and Davidiellaceae (genus Cladosporium), with some samples showing increased levels of Trichocomaceae (genus Aspergillus). The presence of pseudomonads and aspergilli in certain seeds indicates the need for more effective cleaning and sanitation to prevent their impact on the produced oil.
JOURNAL OF FOOD AND NUTRITION RESEARCH
(2023)
Article
Medicine, General & Internal
J. Koren, Z. Hubenakova, H. Drahovska, E. Ozaee, B. Markuskova, A. Lichvarikova
BRATISLAVA MEDICAL JOURNAL-BRATISLAVSKE LEKARSKE LISTY
(2019)
Meeting Abstract
Biochemistry & Molecular Biology
A. Lichvarikova, E. Kolesarova, B. Markuskova, T. Szemes, H. Drahovska, J. Turna
Meeting Abstract
Biochemistry & Molecular Biology
H. Drahovska, M. Andrezal, B. Markuskova, M. Kajsik, J. Turna