Article
Gastroenterology & Hepatology
Hadi Kuriry, Julia Casey, Lisette Krassenburg, Danie La, Magdalena Kuczynski, Hemant Shah, Harry L. A. Janssen, Bettina E. Hansen, Jordan J. Feld
Summary: In patients who relapse after DAA therapy, a small percentage can spontaneously clear HCV infection without additional treatment. These patients typically have low levels of HCV RNA and normal alanine aminotransferase levels at the time of relapse, similar to those who achieve a sustained virologic response to therapy.
CLINICAL GASTROENTEROLOGY AND HEPATOLOGY
(2021)
Article
Medicine, General & Internal
Nevine Bishai, Walid El Nabawy, Mohamed El Fiki, Mohamed Ibrahim, Nouman El Garem
Summary: This study compared the effectiveness of different treatment regimens for chronic HCV patients and found that the regimen using sofosbuvir, daclatasvir, and ribavirin showed the highest cure rate with the least side effects.
IRISH JOURNAL OF MEDICAL SCIENCE
(2023)
Article
Pharmacology & Pharmacy
Dorota Zarebska-Michaluk, Robert Flisiak, Ewa Janczewska, Hanna Berak, Wlodzimierz Mazur, Justyna Janocha-Litwin, Rafal Krygier, Beata Dobracka, Jerzy Jaroszewicz, Anna Parfieniuk-Kowerda, Krystyna Dobrowolska, Piotr Rzymski
Summary: This study assessed the phenomenon of detectable hepatitis C virus (HCV) RNA in patients treated with direct-acting antivirals (DAA) and found that a significant proportion of patients achieved sustained virologic response (SVR) despite detectable HCV RNA at the end of treatment. The use of pangenotypic regimens was associated with a higher rate of achieving SVR in these patients, who had less severe liver disease, were more often infected with genotype (GT) 3, and were more likely to be treatment-naive compared to non-responders.
ANTIVIRAL RESEARCH
(2023)
Article
Cell Biology
Yu Fujita, Tokio Hoshina, Juntaro Matsuzaki, Yusuke Yoshioka, Tsukasa Kadota, Yusuke Hosaka, Shota Fujimoto, Hironori Kawamoto, Naoaki Watanabe, Kenji Sawaki, Yohei Sakamoto, Makiko Miyajima, Kwangyole Lee, Kazuhiko Nakaharai, Tetsuya Horino, Ryo Nakagawa, Jun Araya, Mitsuru Miyato, Masaki Yoshida, Kazuyoshi Kuwano, Takahiro Ochiya
Summary: The study identified EV COPB2 protein as a potential early predictive biomarker for severity of COVID-19, showing excellent predictive value in both discovery and validation cohorts.
JOURNAL OF EXTRACELLULAR VESICLES
(2021)
Article
Immunology
Islam El-Garawani, Sobhy Hassab El-Nabi, Marwa Gadallah, Eman Abdelsameea
Summary: The study aimed to evaluate the predictive value of IFN-lambda 3 gene SNP rs12979860 (C > T) in Egyptian patients with chronic HCV genotype 4 infection treated with DAAs. The TT genotype and T allele were significantly associated with failure to achieve SVR, while the CT genotype may serve as a predictor for SVR in patients receiving DAAs.
IMMUNOLOGICAL INVESTIGATIONS
(2021)
Article
Parasitology
Y. A. Mohammad, A. Mohammed, S. Muath
Summary: Cytokine levels play a role in disease progression, viral survival, and therapeutic response in hepatitis C virus (HCV) infection. This study found significant associations between genetic polymorphisms of IL28B, IFN-gamma, TGF-beta 1, and TNF-alpha and HCV susceptibility and treatment outcome. However, no association was found for IL-6 and IL-10 gene polymorphisms with HCV susceptibility and treatment response.
TROPICAL BIOMEDICINE
(2023)
Article
Physics, Multidisciplinary
Nikita Stasenko, Islomjon Shukhratov, Maxim Savinov, Dmitrii Shadrin, Andrey Somov
Summary: Food quality control is crucial in agriculture to prevent food losses. This article proposes an approach using GAN and CNN techniques to predict decay and fungal zones and assess the quality of stored apples based on synthesized and segmented VNIR imaging data. The achieved results show promise in improving food study and control during the postharvest stage.
Article
Oncology
Zhendong Gao, Han Han, Yue Zhao, Hui Yuan, Shanbo Zheng, Yang Zhang, Haiquan Chen
Summary: An integrated mRNA-ncRNA signature was developed to predict early relapse in early-stage lung adenocarcinoma patients after complete resection. The signature showed strong predictive power and potential utility in guiding treatment strategies, as well as providing insights into chemotherapy sensitivity, tumor microenvironment, and mutation landscape.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2021)
Article
Gastroenterology & Hepatology
Ainhoa Lapitz, Mikel Azkargorta, Piotr Milkiewicz, Paula Olaizola, Ekaterina Zhuravleva, Marit M. Grimsrud, Christoph Schramm, Ander Arbelaiz, Colm J. O'Rourke, Adelaida La Casta, Malgorzata Milkiewicz, Tania Pastor, Mette Vesterhus, Raul Jimenez-Aguero, Michael T. Dill, Angela Lamarca, Juan W. Valle, Rocio I. R. Macias, Laura Izquierdo-Sanchez, Ylenia Perez Castano, Francisco Javier Caballero-Camino, Ioana Riano, Marcin Krawczyk, Cesar Ibarra, Javier Bustamante, Luiz M. Nova-Camacho, Juan M. Falcon-Perez, Felix Elortza, Maria J. Perugorria, Jesper B. Andersen, Luis Bujanda, Tom H. Karlsen, Trine Folseraas, Pedro M. Rodrigues, Jesus M. Banales
Summary: Protein biomarkers for the diagnosis and prognosis of cholangiocarcinoma (CCA) were found in serum extracellular vesicles (EVs), providing important information for individuals at high-risk, such as those with primary sclerosing cholangitis (PSC).
JOURNAL OF HEPATOLOGY
(2023)
Article
Virology
Shabir A. Bhat, Tomohiro Shibata, Matthew Leong, Jasmine Plummer, Eric Vail, Zakir Khan
Summary: This study used RNA-sequencing to analyze the transcriptional response in the upper respiratory tract of COVID-19 patients, and found that severe cases showed an upregulated early type I interferon response and pronounced induction of interferon stimulated genes (ISGs).
Article
Medicine, Research & Experimental
Yue Yin, Ruilan Jiang, Mingwang Shen, Zhaofang Li, Ni Yan, Junqiao Feng, Hong Jiang, Jiaxin Lv, Lijuan Shi, Lina Wang, Xi Liu, Kaiyun Zhang, Di Chen
Summary: This study demonstrates the potential clinical relevance of blood platelets as a platform for liquid biopsy-based early cancer detection, and gene expression signatures were effective predictors for tumor progression in mice.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Daniele Andreini, Eleonora Melotti, Chiara Vavassori, Mattia Chiesa, Luca Piacentini, Edoardo Conte, Saima Mushtaq, Martina Manzoni, Eleonora Cipriani, Paolo M. Ravagnani, Antonio L. Bartorelli, Gualtiero I. Colombo
Summary: This study aimed to identify gene expression patterns in whole blood that could differentiate patients with severe coronary atherosclerosis from subjects with a complete absence of detectable coronary artery disease and to assess associations of gene expression patterns with plaque features in coronary CT angiography (CCTA). The results showed significant differences in the circulating transcriptome between patients with high-degree coronary stenosis and subjects with an absence of coronary plaque. Furthermore, the study suggested that whole-blood transcriptional profiles may predict plaque characteristics.
Article
Parasitology
Maria Fernandez-Alvarez, Pilar Horcajo, Alejandro Jimenez-Melendez, Carlos Diezma-Diaz, Ignacio Ferre, Ivan Pastor-Fernandez, Luis Miguel Ortega-Mora, Gema Alvarez-Garcia
Summary: B. besnoiti-infected bulls may develop severe clinical signs and orchitis, and macrophages play a relevant role in the pathogenesis and immune response. This study focused on the early interaction between B. besnoiti tachyzoites and primary bovine macrophages. Results showed that B. besnoiti can invade and proliferate in macrophages, causing morphological and transcriptomic changes. These findings provide insights into the modulation of macrophages by B. besnoiti and its impact on parasite survival.
INTERNATIONAL JOURNAL FOR PARASITOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Shijie Li, Kerong Xin, Shen Pan, Yang Wang, Jianyi Zheng, Zeyu Li, Xuefeng Liu, Bitian Liu, Zhenqun Xu, Xiaonan Chen
Summary: Bladder cancer is a global clinical challenge characterized by late presentation, poor prognosis, and low survival rates. Traditional methods for diagnosis and monitoring, such as cystoscopy and tissue biopsy, have limitations and high costs. In recent years, liquid biopsies, specifically blood-based ones, have emerged as highly efficient methods for discovering biomarkers and monitoring tumor progression. Several liquid biopsy biomarkers have shown promising results in clinical applications, including early detection, residual disease detection, recurrence prediction, and therapy response.
CELLULAR & MOLECULAR BIOLOGY LETTERS
(2023)
Article
Oncology
Julia Bohosova, Katerina Kozelkova, Dagmar Al Tukmachi, Karolina Trachtova, Ondrej Naar, Michaela Ruckova, Eva Kolarikova, Michal Stanik, Alexandr Poprach, Ondrej Slaby
Summary: In this study, potential biomarkers in the form of long non-coding RNAs (lncRNAs) were identified for renal cell carcinoma using next-generation sequencing. Diagnostic and prognostic models were developed for early diagnosis and prediction of relapse after nephrectomy.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2023)
Article
Pediatrics
Marco Roversi, Umberto Raucci, Giuseppe Pontrelli, Stefania Ranno, Michela Ambrosi, Antonio Torelli, Mara Pisani, Luana Coltella, Livia Piccioni, Luna Colagrossi, Marilena Agosta, Barbara Scialanga, Antonino Reale, Carlo Federico Perno, Paolo Rossi, Alberto Villani
Summary: This study reviewed data of children tested for COVID-19 and found that children without fever or contact with infected individuals were SARS-CoV-2 negative, which could help save resources and improve care.
PEDIATRIC RESEARCH
(2022)
Article
Microbiology
Sara Terreri, Eva Piano Mortari, Maria Rosaria Vinci, Cristina Russo, Claudia Alteri, Christian Albano, Francesca Colavita, Giulia Gramigna, Chiara Agrati, Giulia Linardos, Luana Coltella, Luna Colagrossi, Gloria Deriu, Marta Ciofi Degli Atti, Caterina Rizzo, Marco Scarsella, Rita Brugaletta, Vincenzo Camisa, Annapaola Santoro, Giuseppe Roscilli, Emiliano Pavoni, Alessia Muzi, Nicola Magnavita, Rossana Scutari, Alberto Villani, Massimiliano Raponi, Franco Locatelli, Carlo Federico Perno, Salvatore Zaffina, Rita Carsetti
Summary: This study investigated the long-term and functional B cell memory induced by the BNT162b2 mRNA vaccine against SARS-CoV-2 in healthcare workers. While specific antibodies declined over time, memory B cells persisted and increased, and breakthrough infections did not show signs of waning immunity.
CELL HOST & MICROBE
(2022)
Article
Infectious Diseases
Joseph Fokam, Ezechiel Ngoufack Jagni Semengue, Daniele Armenia, Desire Takou, Beatrice Dambaya, Georges Teto, Collins Ambe Chenwi, Alex Durant Nka, Grace Angong Beloumou, Sandrine Claire Djupsa Ndjeyep, Michel Carlos Tommo Tchouaket, Nadine Fainguem, Samuel Martin Sosso, Vittorio Colizzi, Carlo-Federico Perno, Alexis Ndjolo, Francesa Ceccherini-Silberstein, Maria Mercedes Santoro
Summary: This study developed and validated an integrase genotyping protocol on plasma samples from 195 HIV-infected patients in low and middle-income countries (LMICs). The protocol showed high effectiveness in genotyping both pure and recombinant viral clades, even at low-level viremia.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2022)
Article
Infectious Diseases
Marilena Agosta, Daniela Bencardino, Marta Argentieri, Laura Pansani, Annamaria Sisto, Marta Luisa Ciofi Degli Atti, Carmen D'Amore, Lorenza Putignani, Pietro Bagolan, Barbara Daniela Iacobelli, Andrea Dotta, Ludovica Martini, Luca Di Chiara, Mauro Magnani, Carlo Federico Perno, Francesca Andreoni, Paola Bernaschi
Summary: The spread of carbapenemase-producing Enterobacterales (CPE) is a serious public health threat in pediatric hospitals, especially among newborns who have underdeveloped immune systems and limited treatment options. This study aimed to estimate the prevalence and circulation of CPE in neonatal intensive units of a major pediatric hospital in Italy, and investigate their molecular features. The findings showed that the majority of CPE strains were multidrug-resistant and carried the bla(NDM) gene. There was also a high heterogeneity of CPE strains circulating in neonatal units, with some sharing the same circulating clone.
Article
Public, Environmental & Occupational Health
Maria Elena Flacco, Graziella Soldato, Cecilia Acuti Martellucci, Giuseppe Di Martino, Roberto Carota, Antonio Caponetti, Lamberto Manzoli
Summary: Current data suggests that SARS-CoV-2 reinfections are rare, but uncertainties remain regarding the duration of natural immunity, its protection against the Omicron variant, and the impact of vaccination on reducing reinfection rates. A retrospective cohort analysis of the entire population of an Italian region followed over 1.2 million subjects and found a reinfection rate of 6.1 parts per thousand after an average of 277 days. Importantly, this reinfection rate did not significantly vary over time, suggesting that natural immunity may last beyond 12 months. The risk of reinfection was higher among females, unvaccinated individuals, and during the Omicron wave.
FRONTIERS IN PUBLIC HEALTH
(2022)
Review
Medicine, General & Internal
Maria Elena Flacco, Cecilia Acuti Martellucci, Valentina Baccolini, Corrado De Vito, Erika Renzi, Paolo Villari, Lamberto Manzoli
Summary: This meta-analysis study suggests that the risk of SARS-CoV-2 reinfections is relatively low, but has increased during the first three months of the Omicron wave. There is a strong natural immunity following primary infection, which may last for more than one year. The risk and healthcare needs of recovered individuals might be limited.
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Chemistry, Applied
Federica Castellani, Federico Marini, Giulia Simonetti, Carmela Protano, Leila Fabiani, Lamberto Manzoli, Matteo Vitali
Summary: Concentrations of 7 PCDDs, 10 PCDFs and 18 PCBs were measured in 16 milk samples from sheep and goats in Bussi sul Tirino, central Italy. The samples complied with the maximum levels set in EU regulations for PCDD/Fs and the sum of PCDD/Fs and DL-PCBs. However, in two cases, contamination levels of the sum of PCDD/Fs and DL-PCBs exceeded the action levels recommended by the EU. The contamination profiles of the milk samples were independent of the distance from the illegal landfill but likely influenced by local emission sources.
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2023)
Article
Immunology
Maria Elena Flacco, Cecilia Acuti Martellucci, Graziella Soldato, Giuseppe Di Martino, Roberto Carota, Marco De Benedictis, Graziano Di Marco, Giustino Parruti, Rossano Di Luzio, Antonio Caponetti, Lamberto Manzoli
Summary: This cohort study examined the incidence of potentially vaccine-related serious adverse events (PVR-SAEs) in the entire population of an Italian Province based on COVID-19 vaccination status. The findings showed that vaccinated individuals did not have an increased risk of death or PVR-SAEs compared to the unvaccinated. These results were consistent across different demographics, vaccine types, and infection statuses. Further research is needed to assess the long-term safety of COVID-19 vaccines.
Review
Medicine, General & Internal
Maria Elena Flacco, Cecilia Acuti Martellucci, Valentina Baccolini, Corrado De Vito, Erika Renzi, Paolo Villari, Lamberto Manzoli
Summary: This meta-analysis provides solid evidence that vaccination reduces the risk of SARS-CoV-2 reinfection and severe disease. The results are consistent across different studies and follow-up periods of up to 12 months.
FRONTIERS IN MEDICINE
(2022)
Review
Medicine, General & Internal
Alessandro Ferrieri, Donatella Bosco, Ennio Polilli, Raffaella Ciulli, Lina Visocchi, Lucrezia Mincione, Rosa Iacoe, Rosamaria Zocaro, Antonella Frattari
Summary: This case report presents a rare case of inadvertent intrathecal injection of thiocolchicoside in a healthy woman with persistent low back pain. The patient experienced rapid neurological deterioration after the injection. This highlights the importance of administering injection therapy for low back pain in appropriate settings to promptly address possible complications.
JOURNAL OF MEDICAL CASE REPORTS
(2023)
Article
Virology
Maria Elena Flacco, Cecilia Acuti Martellucci, Graziella Soldato, Giuseppe Di Martino, Annalisa Rosso, Roberto Carota, Marco De Benedictis, Graziano Di Marco, Rossano Di Luzio, Matteo Ricci, Antonio Caponetti, Davide Gori, Lamberto Manzoli
Summary: This cohort study in Italy found that females, older individuals, and those with diabetes, cardiovascular diseases, chronic obstructive pulmonary disease (COPD), kidney disease, and cancer were at a higher risk of SARS-CoV-2 infection. The likelihood of severe or lethal COVID-19 was significantly lower among younger individuals, while comorbidities were independently associated with a higher risk. Vaccination, especially with booster doses, significantly reduced the risk of severe or lethal disease.
Review
Medicine, General & Internal
Francesco D'Antonio, Nashwa Eltaweel, Smriti C. Prasad, Maria Elena C. Flacco, Lamberto C. Manzoli, Asma C. Khalil
Summary: This systematic review and meta-analysis aimed to evaluate the role of cervical cerclage in preventing preterm birth and adverse outcomes in twin pregnancies. The findings suggest that cervical cerclage in twin pregnancies with short cervical length or cervical dilatation is associated with a reduced risk of preterm birth and improved neonatal outcomes. However, confirmation of these findings in large and well-designed randomized controlled trials is needed for stronger evidence.
Article
Immunology
Annalisa Rosso, Maria Elena Flacco, Graziella Soldato, Giuseppe Di Martino, Cecilia Acuti Martellucci, Roberto Carota, Marco De Benedictis, Graziano Di Marco, Rossano Di Luzio, Matteo Fiore, Antonio Caponetti, Lamberto Manzoli
Summary: A cohort study was conducted in the province of Pescara, Italy, to assess the real-world effectiveness of SARS-CoV-2 vaccination. The study found that individuals who received at least one booster dose had an 85% lower risk of severe or lethal COVID-19. Moreover, during the Omicron predominance period, only the group who received at least a booster dose showed a reduced risk of COVID-19-related death.
Meeting Abstract
Hematology
Ennio Polilli, Giustino Parruti, Maria Chiara Anelli, Claudio Silveri, Antonella Lattanzio, Gilda Angelini, Elisabetta Jessica Esposito, Milena D'Amato, Fabrizio Carinci, Giancarlo Di Iorio
INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY
(2023)
Article
Gastroenterology & Hepatology
Anita Y. M. Howe, Chaturaka Rodrigo, Evan B. Cunningham, Mark W. Douglas, Julia Dietz, Jason Grebely, Stephanie Popping, Javier Alejandro Sfalcin, Milosz Parczewski, Christoph Sarrazin, Adolfo de Salazar, Ana Fuentes, Murat Sayan, Josep Quer, Midori Kjellin, Hege Kileng, Orna Mor, Johan Lennerstrand, Slim Fourati, Velia Chiara Di Maio, Vladimir Chulanov, Jean-Michel Pawlotsky, P. Richard Harrigan, Francesca Ceccherini-Silberstein, Federico Garcia
Summary: Drug resistance in HCV is common following DAA treatment failure, with newly identified and uncharacterized substitutions emerging. Multidrug resistance is prevalent in patients with virologic failures, especially those treated with first-generation DAAs. Older individuals with cirrhosis and those infected with genotypes 1b and 4 are more likely to select drug-resistant viruses. International collaborative efforts are needed to effectively manage drug resistance and prevent onward transmission of resistant viruses.
