Journal
ANTIVIRAL RESEARCH
Volume 84, Issue 1, Pages 60-66Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.antiviral.2009.07.004
Keywords
FMDV; IRES; Alpha interferon (IFN-alpha)
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Funding
- State Key Research Fund of China 973 program [2006CB504300]
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Foot-and-mouth disease virus (FMDV) is the most contagious pathogen of cloven-hoofed animals. Previous studies have demonstrated that type I interferons [alpha/beta interferons (IFN-alpha/beta s)] can suppress FMDV replication and spread. Conversely, FMDV can also inhibit IFN-alpha expression in infected cells by blocking cap-dependent translation. To overcome the blockade on IFN-alpha mRNA translation during FMDV infection, we generated an IRES-IFN construct that carries FMDV's internal ribosome entry site (IRES) cDNA sequence between the promoter and porcine IFN-alpha gene. ELISA assays indicated that expression of IFN-alpha regulated by wild-type IRES increased to 125% of pre-infection level after infection for 24 h, but the expression of IFN-alpha regulated by nonfunctional IRES mutants were only similar to 50% of pre-infection level. Correspondingly, the former could suppress the replication of FMDV to 20% of the latter and protect cells against FMDV for a longer time. Therefore, these findings provide a new strategy to anti-FMDV therapy. (C) 2009 Elsevier B.V. All rights reserved.
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