Article
Electrochemistry
Leitao Xu, Yangjie Yi, Sideng Hu, Jiao Ye, Aixi Hu
Summary: This study reports for the first time two pathways for N-hydroxyphthalimide (NHPI)-mediated electrocatalytic oxidation using phenylacetate derivatives as template substrates, achieving benzylic C-H oxidation to oxygenated and non oxygenated products. The rate-determined step was found to be the hydrogen-atom transfer (HAT) process between phthalimido-N-oxyl (PINO) and substrate, as indicated by DFT calculation. By controlling the concentration of PINO radical, selective synthesis of aromatic alpha-keto esters and 2-((1,3dioxoisoindolin-2-yl)oxy)-2-aryl acetate through cross-coupling between benzylic radical and PINO is achieved. This study provides a deep understanding for selective weak C-H oxidation using NHPI as redox mediator.
ELECTROCHIMICA ACTA
(2022)
Editorial Material
Chemistry, Physical
Alberto Boretti
Summary: Wind and solar photovoltaic electricity production has reached low costs, while electrolyzers have high efficiencies and low costs, producing green hydrogen (H-2) from excess renewable energy. H-2 is crucial for grid stability and can be used as fuel for transportation. However, the engineered diagenesis for H-2 has yet to be proven and may have negative environmental and economic impacts.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2023)
Article
Chemistry, Organic
Jingjing Sun, Changying Song, Dongying Ma, Shigang Shen, Shuying Huo
Summary: In this study, L-methionine selenoxide was used as an oxidant for the construction of peptide disulfide bonds, achieving excellent yields for various peptides. The reaction mechanism was found to be first order in both MetSeO and DTT, providing insights into the oxidative synthesis of disulfide bonds. Additionally, the construction of disulfide bonds can be performed during the peptide cleavage process, simplifying the synthesis of disulfide-containing peptides.
JOURNAL OF ORGANIC CHEMISTRY
(2021)
Article
Engineering, Environmental
Yun Hu, Shanyuan Tong, Lihong Hu, Meng Zhang, Qin Huang, Ye Sha, Puyou Jia, Yonghong Zhou
Summary: Dynamic covalent chemistry provides a solution to the recycling problem of epoxy resins and graphene functional composites. In this study, a bio-based epoxy monomer was designed from cardanol to fabricate a double dynamically crosslinked epoxy vitrimer. The resulting bio-based epoxy vitrimers demonstrated advantages such as multi-stimulus responsiveness, multi-channel self-healing ability, mechanical and chemical recovery, and biodegradability.
CHEMICAL ENGINEERING JOURNAL
(2023)
Review
Biotechnology & Applied Microbiology
Tingwei Ren, Zhijun Tan, Vivekh Ehamparanathan, Angela Lewandowski, Sanchayita Ghose, Zheng Jian Li
Summary: Antibody disulfide bond reduction is a challenging issue in monoclonal antibody manufacturing, as it can impact product purity, safety, and efficacy. Scientists have been examining root causes and developing strategies to address this issue. With the increasing demand for antibody biopharmaceuticals, it is crucial to continue evolving mitigation strategies to maintain high product quality.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Engineering, Environmental
Siqin Liu, Zhaoxia Li, Qiangliang Yu, Yuming Qi, Zhenjun Peng, Jun Liang
Summary: This study developed a new self-healing composite coating for corrosion protection of magnesium alloy, utilizing the dual actions of the corrosion inhibitor M-16 in the MAO coating and PU modified by disulfide bonds. Results demonstrated that the inhibitor embedded in the MAO coating significantly improved the anti-corrosion performance of the scratched coating, while the self-healing PU coating showed excellent recovery capability after heat treatment.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Shuang Liu, Shaohui Deng, Xiaoxia Li, Du Cheng
Summary: A copolymer was synthesized for efficient siRNA delivery, with a structure allowing control over the size and surface of polyplexes. The polyplexes exhibited high transfection efficiency and lysosomal escape capacity, showing promise for targeted siRNA delivery.
Article
Nanoscience & Nanotechnology
Kebing Wang, Tengda Shang, Lu Zhang, Lei Zhou, Changqi Liu, Yudie Fu, Yuancong Zhao, Xin Li, Jin Wang
Summary: The study developed an adaptive drug-loaded coating on vascular stent materials that can respond to oxidative stress at the site of vascular lesions, effectively inhibiting inflammation, accelerating endothelialization, and reducing the risk of restenosis.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Inorganic & Nuclear
Li-Juan Sun, Huamin Wang, Jia-Kun Xu, Shu-Qin Gao, Ge-Bo Wen, Ying-Wu Lin
Summary: This study presents the design and construction of a double mutant human neuroglobin (Ngb) that can efficiently catalyze carbene N-H insertions for aromatic amine and o-phenylenediamine derivatives, leading to high yields and total turnover numbers. It also demonstrates the first example of quinoxalinone formation catalyzed by an engineered heme enzyme.
INORGANIC CHEMISTRY
(2023)
Article
Pharmacology & Pharmacy
Zhigao Shen, Cong Liu, Ziqian Wang, Fengfei Xie, Xingwu Liu, Lingkai Dong, Xuehua Pan, Chen Zeng, Peng George Wang
Summary: Researchers developed a series of ionizable lipids with bioreducible disulfide bonds and constructed a lipid library derived from dimercaprol. Lipid nanoparticles (LNPs) prepared from these ionizable lipids can be stored at 4 degrees C for a long term and are non-toxic to HepG2 and 293T cells. In vivo experiments showed that the best C4S18A formulations demonstrated strong firefly luciferase (Fluc) mRNA expression in the liver and spleen via intravenous (IV) injection, or at the local injection site via intramuscular injection (IM). These newly designed ionizable lipids could be potentially safe and high-efficiency nanomaterials for mRNA therapy.
Article
Polymer Science
Yanhua Huang, Jianpan Yan, Dengxu Wang, Shengyu Feng, Chuanjian Zhou
Summary: Novel self-healing disulfide-linked silicone elastomers were developed via thiol oxidation coupling reactions, showing promising self-healing efficiency and reversible bonding capability.
Article
Chemistry, Applied
Giuseppina Sabatino, Annunziata D'Ercole, Lorenzo Pacini, Matteo Zini, Arianna Ribecai, Alfredo Paio, Paolo Rovero, Anna Maria Papini
Summary: The study utilized microwave-assisted solid-phase peptide synthesis to prepare Eptifibatide and explored various solution and solid-phase disulfide formation methods. The research found that the solid-phase strategy optimized the preparation process, resulting in the development of an automated production procedure.
ORGANIC PROCESS RESEARCH & DEVELOPMENT
(2021)
Article
Chemistry, Multidisciplinary
Richard J. Spears, Alina Chrzastek, Steven Y. Yap, Kersti Karu, Abil E. Aliev, James R. Baker, Vijay Chudasama
Summary: In this study, we report a significant discovery regarding the use of tris(dialkylamino)phosphine reagents in peptide and protein modification. We found that C-terminal thiophosphonium species can be selectively and rapidly generated from their disulfide counterparts, with unique stability. In contrast, internal thiophosphonium species degrade quickly. We demonstrated this chemoselectivity on a model peptide and an antibody fragment, and characterized the species in various small molecule/peptide studies.
CHEMICAL COMMUNICATIONS
(2022)
Article
Chemistry, Physical
Lijng Peng, Xiaoguang Duan, Yanan Shang, Baoyu Gao, Xing Xu
Summary: Enteromorpha, a seawater pollutant, was converted into a functional carbocatalyst for Fenton-like reactions. The Enteromorpha-derived Fe-N-C exhibited high activity in activating peroxymonosulfate for organic pollutant degradation. Single Fe atoms played a crucial role as dominant reactive sites in forming highly oxidizing Fe-IV=O and Fe-V=O species.
APPLIED CATALYSIS B-ENVIRONMENTAL
(2021)
Article
Biotechnology & Applied Microbiology
Laura Navone, Thomas Vogl, Pawarisa Luangthongkam, Jo-Anne Blinco, Carlos H. Luna-Flores, Xiaojing Chen, Juhani von Hellens, Stephen Mahler, Robert Speight
Summary: This study presents a thermostable variant of the E. coli AppA phytase, ApV1, with an extra non-consecutive disulfide bond, which shows promising thermostability but leads to decreased production in Pichia pastoris. Co-expression of folding chaperones like protein disulfide bond isomerase (Pdi) significantly increased the production of ApV1 and restored yields to levels comparable with the wild-type enzyme. Engineering for enhanced enzymatic properties may result in folding complexity and reduced production in microbial systems, highlighting the importance of developing improved production strains in parallel to achieve desirable levels of recombinant protein for industrial processes.
BIOTECHNOLOGY FOR BIOFUELS
(2021)