Article
Immunology
Mauro de Mesquita Sousa Saraiva, Valdinete Pereira Benevides, Nubia Michelle Vieira da Silva, Alessandro de Mello Varani, Oliveiro Caetano de Freitas Neto, Angelo Berchieri, Enrique Jesus Delgado-Suarez, Alan Douglas de Lima Rocha, Tadesse Eguale, Janet Agnes Munyalo, Samuel Kariuki, Wondwossen Abebe Gebreyes, Celso Jose Bruno de Oliveira
Summary: MDR Salmonella enterica subsp. enterica serovar Kentucky has emerged as a significant public health problem, especially in East Africa. This study investigated the antimicrobial resistance profile and genotypic relatedness of Salmonella Kentucky isolated from animal sources in Ethiopia and Kenya. Phylogenetic and pangenome analyses with additional publicly available genomes were used to study the evolutionary dynamics of this pathogen. The findings highlighted the importance of investigating the implications of Salmonella Kentucky to public health and its mechanisms of dissemination.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Yue Jiang, Zhen-Yu Wang, Qiu-Chun Li, Meng-Jun Lu, Han Wu, Cai-Yue Mei, Peng-Cheng Shen, Xinan Jiao, Jing Wang
Summary: This study identified extensively drug-resistant Salmonella enterica serovar Kentucky sequence type 198 (ST198) strains from chicken meat products in China. These strains carry numerous resistance genes clustered in the chromosomes, possibly acquired through mobile elements. The emergence and dissemination of extensively drug-resistant S. Kentucky ST198 pose a severe clinical and public health threat, requiring continuous surveillance.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemistry & Molecular Biology
Tomasz Wolkowicz, Katarzyna Zacharczuk, Rafal Gierczynski, Magdalena Nowakowska, Katarzyna Piekarska
Summary: The majority of S. Kentucky isolates from humans in Poland belonged to ST198 and exhibited high-level resistance to ampicillin and quinolones, with multiple resistant determinants found in some strains.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Xenia Vazquez, Javier Fernandez, Margarita Bances, Pilar Lumbreras, Miriam Alkorta, Silvia Hernaez, Elizabeth Prieto, Pedro de la Iglesia, Maria de Toro, M. Rosario Rodicio, Rosaura Rodicio
Summary: This study characterized all ciprofloxacin-resistant Salmonella enterica serovar Kentucky (S. Kentucky) isolates detected in five Spanish hospitals from 2009 to 2018 through whole genome sequencing. The isolates all belonged to sequence type 198 and exhibited resistances to multiple antibiotics mediated by various resistance genes, with a close genetic relationship observed between isolates from different hospitals.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Sandra Sanchez-Urtaza, Alain Ocampo-Sosa, Ainhoa Molins-Bengoetxea, Jorge Rodriguez-Grande, Mohammed A. El-Kholy, Marta Hernandez, David Abad, Sherine M. Shawky, Itziar Alkorta, Lucia Gallego
Summary: The increasing rates of antimicrobial resistance among carbapenem-resistant Acinetobacter baumannii in the Middle East and North Africa are a major concern. The first A. baumannii isolate with five β-lactamases was identified in Egypt. This isolate exhibited resistance to multiple antibiotics and carried genes associated with virulence and biofilm production.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Infectious Diseases
Jacqueline Findlay, Vincent Perreten, Laurent Poirel, Patrice Nordmann
Summary: OXA-48-type β-lactamases are the most common carbapenemase-type in Enterobacterales in Switzerland, mainly found in Escherichia coli and Klebsiella pneumoniae. Bacteria producing OXA-48-type enzymes are endemic in certain regions, including Europe and North Africa, and frequently cause hospital-acquired infections. Despite the emergence of multiple OXA-48-type variants, the original variant, OXA-48, remains the most prevalent in E. coli. This study describes the epidemiology of OXA-48-producing E. coli isolates submitted to the Swiss National Reference Center for Emerging Antibiotic Resistance (NARA) between January 2019 and December 2020.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2022)
Article
Multidisciplinary Sciences
Kamilla B. Alieva, Maria N. Golikova, Svetlana A. Dovzhenko, Mikhail A. Kobrin, Elena S. Strukova, Vladimir V. Ageevets, Alisa H. Avdeeva, Ofeliia Sulian, Sergey Sidorenko, Stephen Zinner
Summary: OXA-48 carbapenemases are frequently expressed by Klebsiella pneumoniae clinical isolates and decrease the effectiveness of meropenem therapy. The emergence of resistance in susceptible carbapenemase-producing isolates and their resistance patterns when exposed to meropenem are not completely understood. This study confirmed the applicability of the mutant selection window (MSW) hypothesis to meropenem and carbapenemase-producing K. pneumoniae.
Article
Infectious Diseases
O. Lima, A. Sousa, R. Longueira-Suarez, A. Filgueira, C. Taboada-Martinez, C. Portela-Pino, A. Nodar, F. Vasallo-Vidal, L. Martinez-Lamas, A. Perez-Landeiro, M. Rubianes, M. T. Perez-Rodriguez
Summary: This study aimed to analyze the clinical efficacy of CAZ-AVI compared with BAT in patients with CRKp-OXA-48 bacteremia. The results showed that CAZ-AVI treatment might be associated with a higher clinical success rate, especially in patients younger than 70 years old.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2022)
Article
Infectious Diseases
Jacqueline Findlay, Patrice Nordmann, Maxime Bouvier, Auriane Kerbol, Laurent Poirel
Summary: This study investigated the spread and emergence of A. baumannii clones co-producing OXA-23 and ArmA in Switzerland. The majority of the isolates were obtained from infections and belonged to the high-risk GC-2 clone. Monitoring and control of these MDR A. baumannii strains are crucial to prevent further spread.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2023)
Article
Infectious Diseases
Yaling Li, Kaiyang Shao, Ruyi Cai, Yi Liu, Xiaojing Liu, Feihua Ni, Huiyan Zheng, Ruying Hu, Ting Sun
Summary: In this study, a multidrug-resistant P. rettgeri strain YQ150713 was found to carry the blaNDM-1 gene co-located with blaOXA-10 on a novel plasmid pYQ150713-NDM-1 with a horizontal transfer function. More surveillance will be required in the future to reduce the dissemination risk of such P. rettgeri isolates in clinical settings.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Public, Environmental & Occupational Health
Rosemarie Slowey, Seon Woo Kim, Deirdre Prendergast, Gillian Madigan, Jo Ann S. Van Kessel, Bradd J. Haley
Summary: The study investigated the genetic diversity and antimicrobial sensitivity patterns of S. Kentucky strains isolated from non-human sources in Ireland. Most isolates belonged to ST314 and only a few were antimicrobial-resistant. The presence of multidrug-resistant ST198 in food sources in Ireland was also identified.
ZOONOSES AND PUBLIC HEALTH
(2022)
Article
Microbiology
Lei Fang, Guankai Lin, Yi Li, Qiange Lin, Huihuang Lou, Meifeng Lin, Yuqin Hu, Airong Xie, Qinyi Zhang, Jiancang Zhou, Leyi Zhang
Summary: Increasing human salmonellosis caused by Salmonella enterica serovar Kentucky and London has raised concerns. This study examined the genetic traits and antimicrobial resistance of these serovars in Zhejiang Province, China. The findings revealed diversity in host range and disease-causing potential, as well as similarities between food and clinical isolates. Additionally, high-level dual resistance to ciprofloxacin and cephalosporins in S. Kentucky ST198 isolates highlights the evolving threat of antibiotic resistance.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Jule Anna Horlbog, Roger Stephan, Marc J. A. Stevens, Gudrun Overesch, Sonja Kittl, Maira Napoleoni, Valentina Silenzi, Magdalena Nueesch-Inderbinen, Sarah Albini
Summary: Poultry feed is a major source of Salmonella infection in poultry in Switzerland. Organic feed is typically only treated with organic acids, and the detection of the same type of Salmonella in both Italy and Switzerland suggests a potential outbreak risk.
Article
Veterinary Sciences
Mujtaba Hassan, Aamir Ali, Abrar Ahmad, Muhammad Kashif Saleemi, Muhammad Wajid, Yasra Sarwar, Mazhar Iqbal
Summary: The genus Salmonella, including the clinically important Salmonella enterica species, causes infections in both humans and animals, especially in poultry, posing a major public health concern. Improving sanitation and hygiene is crucial to control the disease, but precise diagnosis and vaccination are essential in densely populated developing countries like Pakistan. In this study, Salmonella enterica serovar Typhimurium isolates were identified and molecularly confirmed, with the goal of developing a polysaccharide-protein based conjugate vaccine against the local S. Typhimurium isolate.
PAKISTAN VETERINARY JOURNAL
(2021)
Article
Biotechnology & Applied Microbiology
Sophia Inbaraj, Ravi Kant Agrawal, Prasad Thomas, Pallab Chaudhuri, V. K. Chaturvedi
Summary: This study aimed to isolate and characterize a bacteriophage against Salmonella enterica serovar Kentucky and evaluate its effectiveness in decontaminating chicken skin. The bacteriophage belonged to the genus chivirus and exhibited high susceptibility against multidrug-resistant S. enterica isolates. The results revealed that high multiplicity of infection is required for significant reduction of bacterial concentration.
FOLIA MICROBIOLOGICA
(2023)
Review
Infectious Diseases
Edgar I. Campos-Madueno, Melika Moradi, Yasmine Eddoubaji, Fatemeh Shahi, Sina Moradi, Odette J. Bernasconi, Aline I. Moser, Andrea Endimiani
Summary: Infections caused by ESBL- and/or carbapenemase-producing Enterobacterales (Ent) have had a significant clinical impact globally. These multidrug-resistant (MDR) pathogens, particularly Escherichia coli and Klebsiella pneumoniae, can cause infections originating from asymptomatic intestinal colonization, leading to potential transmission. Rapid identification of gut carriers is important for preventing serious infections and transmission. This review summarizes various aspects of intestinal colonization by MDR-Ent, including screening techniques, prevalence and risk factors, clinical impact, colonization duration, and strategies for decolonization.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2023)
Article
Microbiology
Aline I. Moser, Yasmine Eddoubaji, Edgar I. Campos-Madueno, Andrea Endimiani
Summary: This study presents the complete genome sequence of Entomomonas sp. E2T0, a strain isolated from larvae of the darkling beetle Zophobas morio. The isolate was found to be fully resistant to aztreonam and carried a novel class D beta-lactamase gene. The genome consists of a chromosome and a plasmid, totaling 3,325,929 base pairs.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Article
Microbiology
Edgar I. I. Campos-Madueno, Aline I. I. Moser, Peter M. M. Keller, Vincent Perreten, Laurent Poirel, Patrice Nordmann, Andrea Endimiani
Summary: KoC species overproduce OXY beta-lactamases, leading to reduced susceptibility to piperacillin-tazobactam, expanded-spectrum cephalosporins, and aztreonam. Whole-genome sequencing and susceptibility testing were used to characterize 44 KoC strains, showing phenotypic differences between ESBL producers and hyperproducers of OXYs. Only cefepime/cefepime-clavulanate combination disk tests or the double-disk synergy test could accurately distinguish between these two groups.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Letter
Infectious Diseases
Jennifer Eleonora Keller, Sybille Schwendener, Dana Novakova, Roman Pantucek, Vincent Perreten
MICROBIAL DRUG RESISTANCE
(2023)
Article
Infectious Diseases
Ervin Albert, Rita Sipos, Vincent Perreten, Akos Toth, Erika Ungvari, Marton Papp, Adam Dan, Imre Biksi
Summary: In Hungary, a study found that 83% of pig farms and 70% of swine professionals were carriers of Livestock-Associated Methicillin-Resistant Staphylococcus aureus (LA-MRSA). The study highlights the significant increase in the occurrence of LA-MRSA in the swine industry in Hungary, calling for a reassessment of the risk it poses to public health.
TRANSBOUNDARY AND EMERGING DISEASES
(2023)
Article
Genetics & Heredity
Aitana Neves, Daniel Walther, Trinidad Martin-Campos, Valerie Barbie, Claire Bertelli, Dominique Blanc, Gerard Bouchet, Frederic Erard, Gilbert Greub, Hans H. Hirsch, Michael Huber, Laurent Kaiser, Stephen L. Leib, Karoline Leuzinger, Vladimir Lazarevic, Mirjam Mausezahl, Jorge Molina, Richard A. Neher, Vincent Perreten, Alban Ramette, Tim Roloff, Jacques Schrenzel, Helena M. B. Seth-Smith, Roger Stephan, Dillenn Terumalai, Fanny Wegner, Adrian Egli
Summary: The SPSP is a secure platform that enables surveillance and monitoring of pathogens in human, veterinary, environmental, and foodborne sources. It utilizes whole genome sequencing data and metadata to quickly track and monitor the transmission and outbreak of pathogens. It provides controlled data access, complex dynamic queries, dedicated dashboards, and automated data sharing with international repositories, aiming to improve public health and societal well-being.
MICROBIAL GENOMICS
(2023)
Article
Microbiology
Edgar I. I. Campos-Madueno, Claudia Aldeia, Parham Sendi, Andrea Endimiani
Summary: Escherichia ruysiae is a newly discovered bacteria commonly found in animals and the environment. It has the potential to colonize the human intestinal tract and carry antibiotic resistance genes, emphasizing the need for monitoring and research.
MICROBIOLOGY SPECTRUM
(2023)
Article
Infectious Diseases
Samuel Raemy, Carlo Casanova, Rossella Baldan, Erin Barreto, Aaron J. Tande, Andrea Endimiani, Stephen L. Leib, Urs Fischer, Parham Sendi
Summary: The recommended dosing regimen for acute bacterial meningitis in adults with penicillin-susceptible Streptococcus pneumoniae is either 2 g every 12 h or a single dose of 2 g every 24 h. This study aimed to evaluate ceftriaxone susceptibility and clinical outcomes following different dosing regimens. The results showed that all isolates were susceptible to ceftriaxone and there was no statistical difference in outcome between the two dosing regimens.
Article
Microbiology
Claudia Aldeia, Edgar I. Campos-Madueno, Parham Sendi, Andrea Endimiani
Summary: We report the complete genome sequence of a colistin-resistant Raoultella electrica strain (MIC, >4 μg/mL) isolated from the stool of a healthy individual in India. The genome consists of a chromosome and three plasmids (5,455,992-bp and 98,913-bp, 4,232-bp, and 3,961-bp, respectively). No previously known colistin resistance mechanisms were identified.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Article
Microbiology
Andrew F. Brown, Judith Pfister, Javier E. Fernandez, Valentina Dona, Victor Rodriguez, Gina Retschnig, Alexandra Collaud, Peter Neumann, Vincent Perreten
Summary: The complete genome sequence of Lactobacillus melliventris strain IBH004, isolated from the gut of a honeybee worker (Apis mellifera) and containing two plasmids and a temperate phage, was determined using hybrid assembly of Oxford Nanopore and Illumina reads. Phage-sequence relationships were identified from the coding sequences, and a proteomic tree was constructed.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Article
Virology
Parham Sendi, Nadja Widmer, Mattia Branca, Marc Thierstein, Annina Elisabeth Buchi, Dominik Guntensperger, Manuel Raphael Blum, Rossella Baldan, Caroline Tinguely, Dik S. Heg, Elitza Theel, Elie J. Berbari, Aaron Tande, Andrea Endimiani, Peter Gowland, Christoph Niederhauser
Summary: In a cohort study, researchers examined the factors associated with changes in anti-S1 IgG antibody levels over time in vaccinated individuals. They found that higher antibody levels were associated with protection against SARS-CoV-2 infection, with a stronger association during the Omicron period. However, the impact of these antibody levels on predicting infection protection was limited.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Microbiology
Emma Marchionatti, Vincent Perreten
Summary: A novel 23S ribosomal RNA methylase gene erm(56) flanked by insertion sequence IS6100 was identified in a Trueperella pyogenes isolated from the abscess of a dog and was also present in another T. pyogenes and in Rothia nasimurium from livestock. It was shown to confer resistance to macrolide, lincosamide, streptogramin B antibiotics in T. pyogenes and E. coli, indicating functionality in both Gram-positive and Gram-negative bacteria. The detection of erm(56) on different elements in unrelated bacteria from different animal sources and geographical origins suggests that it has been independently acquired and likely selected by the use of antibiotics in animals.
Article
Infectious Diseases
Valentina Dona, Patrice Nordmann, Sonja Kittl, Simone Schuller, Maxime Bouvier, Laurent Poirel, Andrea Endimiani, Vincent Perreten
Summary: A study in Switzerland found that Enterobacter hormaechei producing the carbapenemase OXA-48 was identified in companion animals and human patients. The study analyzed the genetic relatedness of these strains and their mobile genetic elements and found a connection between antimicrobial resistance in E. hormaechei and animals and humans in the same environment.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)