Article
Microbiology
Dara Kiani, William Santus, Kaitlyn A. Kiernan, Judith Behnsen
Summary: A novel contact-dependent competition system for Proteus mirabilis was identified, which efficiently reduced the viability of several Enterobacteriaceae species. The killing system may represent a new function of a known competition system or a novel system, as it does not fit with described contact-dependent competition systems. Further characterization of this system may help understand how P. mirabilis competes with other Enterobacteriaceae in various niches.
Article
Veterinary Sciences
Kainat Ishaq, Aqsa Ahmad, Azhar Rafique, Rizwan Aslam, Sultan Ali, M. Akbar Shahid, Noreen Sarwar, M. Aamir Aslam, Bilal Aslam, Muhammad Imran Arshad
Summary: This study investigated the prevalence of Proteus mirabilis in poultry meat and its antimicrobial susceptibility. The results showed a high positivity rate of P. mirabilis in chicken carcass, and the isolates exhibited resistance to certain antibiotics. The study suggests that chicken meat could serve as a source of this pathogen and potentially infect humans and animals.
PAKISTAN VETERINARY JOURNAL
(2022)
Article
Infectious Diseases
Matheus Silva Sanches, Luana Carvalho Silva, Caroline Rodrigues da Silva, Victor Hugo Montini, Bruno Henrique Dias de Oliva, Gustavo Henrique Migliorini Guidone, Mara Correa Lelles Nogueira, Maisa Fabiana Menck-Costa, Renata Katsuko Takayama Kobayashi, Eliana Carolina Vespero, Sergio Paulo Dejato Rocha
Summary: The study aimed to evaluate the prevalence of antimicrobial resistance and clonal relationships in Proteus mirabilis isolated from different sources. Results showed that chicken meat had the highest multidrug resistance, followed by pork and community-acquired urinary tract infections (UTI-CA), while beef had relatively few multidrug-resistant strains. Various antimicrobial resistance genes were detected, especially in chicken meat and UTI-CA isolates. Molecular typing revealed genetic similarity between chicken meat and UTI-CA isolates, indicating that chicken could be a source of P. mirabilis causing UTI-CA. It was concluded that meat, especially chicken, can be an important source of multidrug-resistant P. mirabilis dissemination in the community.
Article
Infectious Diseases
Marion Lecuru, Marie-Helene Nicolas-Chanoine, Sebastien Tanaka, Philippe Montravers, Laurence Armand-Lefevre, Erick Denamur, Hedi Mammeri
Summary: The study found that the clinical isolates PmirS and PmirR of Proteus mirabilis were almost genetically identical, but the mutated cpxA gene in PmirR led to resistance. This research reveals that alterations in CpxA may confer imipenem and amikacin resistance in P. mirabilis without affecting its growth rate.
MICROBIAL DRUG RESISTANCE
(2021)
Article
Infectious Diseases
Zana Rubic, Silvija Soprek, Marko Jelic, Anita Novak, Ivana Goic-Barisic, Marina Radic, Arjana Tambic-Andrasevic, Marija Tonkic
Summary: This study investigated the genetic relatedness and molecular resistance mechanisms of AmpC-producing multidrug-resistant Proteus mirabilis isolates in a university hospital. The isolates showed resistance to various antibiotics and carried different resistance genes. Alternative antibiotics were found to be efficient in vitro against these isolates, suggesting potential for sparing carbapenem usage.
MICROBIAL DRUG RESISTANCE
(2021)
Article
Biotechnology & Applied Microbiology
Arezoo Mirzaei, Bahram Nasr Esfahani, Abbasali Raz, Mustafa Ghanadian, Sharareh Moghim
Summary: The study revealed that Proteus mirabilis isolates commonly possess the ability to form biofilms and exhibit multidrug resistance, particularly to trimethoprim-sulfamethoxazole. Among the integron and ESBL gene family, intI1 and bla(TEM) were the most prevalent genes detected. High diversity was observed in the isolates based on Rep-PCR analysis.
BIOMED RESEARCH INTERNATIONAL
(2021)
Review
Infectious Diseases
Edgar I. Campos-Madueno, Melika Moradi, Yasmine Eddoubaji, Fatemeh Shahi, Sina Moradi, Odette J. Bernasconi, Aline I. Moser, Andrea Endimiani
Summary: Infections caused by ESBL- and/or carbapenemase-producing Enterobacterales (Ent) have had a significant clinical impact globally. These multidrug-resistant (MDR) pathogens, particularly Escherichia coli and Klebsiella pneumoniae, can cause infections originating from asymptomatic intestinal colonization, leading to potential transmission. Rapid identification of gut carriers is important for preventing serious infections and transmission. This review summarizes various aspects of intestinal colonization by MDR-Ent, including screening techniques, prevalence and risk factors, clinical impact, colonization duration, and strategies for decolonization.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2023)
Article
Microbiology
Aline I. Moser, Yasmine Eddoubaji, Edgar I. Campos-Madueno, Andrea Endimiani
Summary: This study presents the complete genome sequence of Entomomonas sp. E2T0, a strain isolated from larvae of the darkling beetle Zophobas morio. The isolate was found to be fully resistant to aztreonam and carried a novel class D beta-lactamase gene. The genome consists of a chromosome and a plasmid, totaling 3,325,929 base pairs.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Article
Microbiology
Edgar I. I. Campos-Madueno, Aline I. I. Moser, Peter M. M. Keller, Vincent Perreten, Laurent Poirel, Patrice Nordmann, Andrea Endimiani
Summary: KoC species overproduce OXY beta-lactamases, leading to reduced susceptibility to piperacillin-tazobactam, expanded-spectrum cephalosporins, and aztreonam. Whole-genome sequencing and susceptibility testing were used to characterize 44 KoC strains, showing phenotypic differences between ESBL producers and hyperproducers of OXYs. Only cefepime/cefepime-clavulanate combination disk tests or the double-disk synergy test could accurately distinguish between these two groups.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Letter
Infectious Diseases
Jennifer Eleonora Keller, Sybille Schwendener, Dana Novakova, Roman Pantucek, Vincent Perreten
MICROBIAL DRUG RESISTANCE
(2023)
Article
Infectious Diseases
Ervin Albert, Rita Sipos, Vincent Perreten, Akos Toth, Erika Ungvari, Marton Papp, Adam Dan, Imre Biksi
Summary: In Hungary, a study found that 83% of pig farms and 70% of swine professionals were carriers of Livestock-Associated Methicillin-Resistant Staphylococcus aureus (LA-MRSA). The study highlights the significant increase in the occurrence of LA-MRSA in the swine industry in Hungary, calling for a reassessment of the risk it poses to public health.
TRANSBOUNDARY AND EMERGING DISEASES
(2023)
Article
Genetics & Heredity
Aitana Neves, Daniel Walther, Trinidad Martin-Campos, Valerie Barbie, Claire Bertelli, Dominique Blanc, Gerard Bouchet, Frederic Erard, Gilbert Greub, Hans H. Hirsch, Michael Huber, Laurent Kaiser, Stephen L. Leib, Karoline Leuzinger, Vladimir Lazarevic, Mirjam Mausezahl, Jorge Molina, Richard A. Neher, Vincent Perreten, Alban Ramette, Tim Roloff, Jacques Schrenzel, Helena M. B. Seth-Smith, Roger Stephan, Dillenn Terumalai, Fanny Wegner, Adrian Egli
Summary: The SPSP is a secure platform that enables surveillance and monitoring of pathogens in human, veterinary, environmental, and foodborne sources. It utilizes whole genome sequencing data and metadata to quickly track and monitor the transmission and outbreak of pathogens. It provides controlled data access, complex dynamic queries, dedicated dashboards, and automated data sharing with international repositories, aiming to improve public health and societal well-being.
MICROBIAL GENOMICS
(2023)
Article
Microbiology
Edgar I. I. Campos-Madueno, Claudia Aldeia, Parham Sendi, Andrea Endimiani
Summary: Escherichia ruysiae is a newly discovered bacteria commonly found in animals and the environment. It has the potential to colonize the human intestinal tract and carry antibiotic resistance genes, emphasizing the need for monitoring and research.
MICROBIOLOGY SPECTRUM
(2023)
Article
Infectious Diseases
Samuel Raemy, Carlo Casanova, Rossella Baldan, Erin Barreto, Aaron J. Tande, Andrea Endimiani, Stephen L. Leib, Urs Fischer, Parham Sendi
Summary: The recommended dosing regimen for acute bacterial meningitis in adults with penicillin-susceptible Streptococcus pneumoniae is either 2 g every 12 h or a single dose of 2 g every 24 h. This study aimed to evaluate ceftriaxone susceptibility and clinical outcomes following different dosing regimens. The results showed that all isolates were susceptible to ceftriaxone and there was no statistical difference in outcome between the two dosing regimens.
Article
Microbiology
Claudia Aldeia, Edgar I. Campos-Madueno, Parham Sendi, Andrea Endimiani
Summary: We report the complete genome sequence of a colistin-resistant Raoultella electrica strain (MIC, >4 μg/mL) isolated from the stool of a healthy individual in India. The genome consists of a chromosome and three plasmids (5,455,992-bp and 98,913-bp, 4,232-bp, and 3,961-bp, respectively). No previously known colistin resistance mechanisms were identified.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Article
Microbiology
Andrew F. Brown, Judith Pfister, Javier E. Fernandez, Valentina Dona, Victor Rodriguez, Gina Retschnig, Alexandra Collaud, Peter Neumann, Vincent Perreten
Summary: The complete genome sequence of Lactobacillus melliventris strain IBH004, isolated from the gut of a honeybee worker (Apis mellifera) and containing two plasmids and a temperate phage, was determined using hybrid assembly of Oxford Nanopore and Illumina reads. Phage-sequence relationships were identified from the coding sequences, and a proteomic tree was constructed.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Article
Virology
Parham Sendi, Nadja Widmer, Mattia Branca, Marc Thierstein, Annina Elisabeth Buchi, Dominik Guntensperger, Manuel Raphael Blum, Rossella Baldan, Caroline Tinguely, Dik S. Heg, Elitza Theel, Elie J. Berbari, Aaron Tande, Andrea Endimiani, Peter Gowland, Christoph Niederhauser
Summary: In a cohort study, researchers examined the factors associated with changes in anti-S1 IgG antibody levels over time in vaccinated individuals. They found that higher antibody levels were associated with protection against SARS-CoV-2 infection, with a stronger association during the Omicron period. However, the impact of these antibody levels on predicting infection protection was limited.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Microbiology
Emma Marchionatti, Vincent Perreten
Summary: A novel 23S ribosomal RNA methylase gene erm(56) flanked by insertion sequence IS6100 was identified in a Trueperella pyogenes isolated from the abscess of a dog and was also present in another T. pyogenes and in Rothia nasimurium from livestock. It was shown to confer resistance to macrolide, lincosamide, streptogramin B antibiotics in T. pyogenes and E. coli, indicating functionality in both Gram-positive and Gram-negative bacteria. The detection of erm(56) on different elements in unrelated bacteria from different animal sources and geographical origins suggests that it has been independently acquired and likely selected by the use of antibiotics in animals.
Article
Infectious Diseases
Valentina Dona, Patrice Nordmann, Sonja Kittl, Simone Schuller, Maxime Bouvier, Laurent Poirel, Andrea Endimiani, Vincent Perreten
Summary: A study in Switzerland found that Enterobacter hormaechei producing the carbapenemase OXA-48 was identified in companion animals and human patients. The study analyzed the genetic relatedness of these strains and their mobile genetic elements and found a connection between antimicrobial resistance in E. hormaechei and animals and humans in the same environment.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)