Article
Infectious Diseases
Kei Ikarashi, Ryo Kutsuna, Junko Tomida, Yoshiaki Kawamura, Yuji Morita
Summary: The study found that MexXY overexpression and deficiency in multidrug-resistant Pseudomonas aeruginosa can impact pyoverdine production, with MexXY deficiency leading to increased pyoverdine production. MexXY-deficient mutants may be more susceptible to iron deficiency, while high-risk clones are usually MexXY overproducers but defective pyoverdine producers.
Article
Microbiology
Zheng Fan, Xiaolei Pan, Dan Wang, Ronghao Chen, Tongtong Fu, Baopeng Yang, Yongxin Jin, Fang Bai, Zhihui Cheng, Weihui Wu
Summary: PNPase mutation in Pseudomonas aeruginosa increases bacterial tolerance to aminoglycoside antibiotics, with the upregulation of MexXY genes being responsible for this increase in tolerance. Additionally, PNPase controls the translation of armZ mRNA, which in turn affects the expression of the MexXY efflux pump.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Microbiology
Giancarlo Atassi, Rachel Medernach, Marc Scheetz, Sophia Nozick, Nathaniel J. Rhodes, Megan Murphy-Belcaster, Katherine R. Murphy, Arghavan Alisoltani, Egon A. Ozer, Alan R. Hauser
Summary: Resistance to aminoglycosides in Pseudomonas aeruginosa remained constant over 2 decades, indicating the effectiveness of antibiotic stewardship programs. Clinically relevant AME genes were found in 14% of isolates, and mutations in mexZ and armZ genes were common. These findings highlight the ongoing problem of aminoglycoside resistance and suggest potential targets for novel therapeutics.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemistry & Molecular Biology
Giorgia Giorgini, Gianmarco Mangiaterra, Nicholas Cedraro, Emiliano Laudadio, Giulia Sabbatini, Mattia Cantarini, Cristina Minnelli, Giovanna Mobbili, Emanuela Frangipani, Francesca Biavasco, Roberta Galeazzi
Summary: Berberine and its derivatives show synergistic effects with tobramycin against different strains of Pseudomonas aeruginosa, leading to varying degrees of MIC reduction and killing effect. This suggests their potential as MexXY-OprM inhibitors for the rational design of novel agents.
Article
Infectious Diseases
Alexandre Tetard, Susie Gaillot, Eline Dubois, Soumaya Aarras, Benoit Valot, Gilles Phan, Patrick Plesiat, Catherine Llanes
Summary: Research has found that Pseudomonas aeruginosa is able to adapt to the strong electrophilic molecule cinnamaldehyde (CNA) by upregulating its intrinsic efflux pump and through other pleiotropic changes, leading to increased resistance. Further evaluation is needed to assess whether multidrug-resistant mutants can emerge in patients using cinnamon essential oil as self-medication.
Article
Microbiology
Xiaolei Pan, Xinrui Zhao, Yuqin Song, Huan Ren, Zhenyang Tian, Qi'an Liang, Yongxin Jin, Fang Bai, Zhihui Cheng, Jie Feng, Weihui Wu
Summary: This study found that Pseudomonas aeruginosa developed resistance to WCK 5222, and identified mutations in the zidebactam target protein PBP2 as the major cause of resistance.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Punyawee Dulyayangkul, Naphat Satapoomin, Matthew B. Avison, Nisanart Charoenlap, Paiboon Vattanaviboon, Skorn Mongkolsuk
Summary: The study found that over-expression of MFS pumps in Pseudomonas aeruginosa can lead to over-expression of RND pumps, specifically MexXY, resulting in reduced susceptibility to aminoglycosides, quinolones, and paraquat. This cascade effect was found to be dependent on increased armZ expression. In addition, it was discovered that changes in paraquat susceptibility were independent of mexXY and armZ, indicating that paraquat is a substrate of MFS pumps Mfs1 and Mfs2.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Nuoyan Wang, Xuemin Chen, Jing Luo, Fei Deng, Fuguo Shi, Qin Wu, Yasi Huang, Qin Ouyang, Rongxin Qin, Hong Zhou
Summary: This study investigated the antibacterial sensitization effect of DHA27 combined with tobramycin in tobramycin-resistant Pseudomonas aeruginosa. The combination showed significant antibacterial effects on many resistant strains and reduced bacterial load in the spleen and lungs in a mouse model. The study also revealed that the effect was related to the inhibition of mRNA expression of aminoglycoside-modifying enzymes.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Microbiology
Nitasha D. Menon, Samuel Penziner, Elizabeth T. Montano, Raymond Zurich, David T. Pride, Bipin G. Nair, Geetha B. Kumar, Victor Nizet
Summary: Bacteriophage therapy is an alternative treatment to antibiotics for multidrug-resistant pathogens. This study found that phage therapy can lead to the emergence of phage-resistant mutants with pyomelanin pigmentation, but these mutants are less virulent due to large genomic deletions and retain susceptibility to the antibiotic colistin. This suggests that they do not pose a contraindication to using anti-pseudomonal phage therapy.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Microbiology
Qin Dou, Yuxiang Zhu, Chunhui Li, Zeran Bian, Huihui Song, Ruizhen Zhang, Yingsong Wang, Xile Zhang, Yan Wang
Summary: The combination of halogenated indoles and aminoglycoside antibiotics can enhance the effectiveness against Pseudomonas aeruginosa PAO1 and uncover the mechanism of the regulatory effect induced by 4F-indole.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Mahsa Ghonouei Rastgar, Behnam Rasti, Hojjatolah Zamani
Summary: This study investigated the effect of ibuprofen on biofilm formation and the expression of pelD and pelF genes in pathogenic Pseudomonas aeruginosa strains. The results showed that ibuprofen significantly reduced biofilm formation and attenuated the expression of pelD and pelF genes.
ARCHIVES OF MICROBIOLOGY
(2022)
Review
Chemistry, Multidisciplinary
Christian Hobson, Andrew N. Chan, Gerard D. Wright
Summary: The ongoing challenge of antibiotic resistance is a formidable threat towards the discovery and use of traditional antibiotics. The study and understanding of molecular mechanisms in the resistome provide a basis for the genomics-driven discovery and development of novel antimicrobials to combat emerging pathogens that are resistant to even the newest approved therapies.
Article
Infectious Diseases
Aswin Thacharodi, Iain L. Lamont
Summary: Bioinformatics analysis identified multiple aminoglycoside-modifying enzymes in P. aeruginosa, which significantly impact the antibiotic susceptibility of the bacteria and can even turn originally susceptible strains into resistant ones.
Article
Infectious Diseases
Emma Taylor, Elita Jauneikaite, Shiranee Sriskandan, Neil Woodford, Katie L. Hopkins
Summary: The presence of 16S RMTase genes in Pseudomonas aeruginosa isolates contributes to high-level resistance to aminoglycosides. These genes were found in 8.6% of the isolates, with rmtB4 being the most common. The study also identified a connection between 16S RMTase genes, carbapenemase-producing bacteria, and high-risk clones.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2022)
Article
Microbiology
Madeline Mei, Preston Pheng, Detriana Kurzeja-Edwards, Stephen P. Diggle
Summary: Chronic, highly antibiotic-resistant infections in cystic fibrosis lungs contribute to increasing morbidity and mortality. Pseudomonas aeruginosa, a common CF pathogen, exhibits resistance to multiple antibiotics, contributing to antimicrobial resistance. This study found that the diversity of P. aeruginosa variants within CF lung infections leads to varying susceptibility to R-pyocins, and suggests that LPS packing density may play a significant role in R-pyocin susceptibility among CF variants.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Keith Poole, Calvin Ho-Fung Lau, Christie Gilmour, Youai Hao, Joseph S. Lam
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2015)
Article
Microbiology
Keith Poole, Christie Gilmour, Maya A. Farha, Erin Mullen, Calvin Ho-Fung Lau, Eric D. Brown
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2016)
Article
Environmental Sciences
Calvin Ho-Fung Lau, Bing Li, Tong Zhang, Yuan-Ching Tien, Andrew Scott, Roger Murray, Lyne Sabourin, David R. Lapen, Peter Duenk, Edward Topp
SCIENCE OF THE TOTAL ENVIRONMENT
(2017)
Letter
Microbiology
Calvin Ho-Fung Lau, Edward Topp
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2018)
Article
Multidisciplinary Sciences
Thomas Hay, Sebastien Fraud, Calvin Ho-Fung Lau, Christie Gilmour, Keith Poole
Article
Microbiology
Calvin Ho-Fung Lau, Daniel Hughes, Keith Poole
Article
Microbiology
Calvin Ho-Fung Lau, Thomas Krahn, Christie Gilmour, Erin Mullen, Keith Poole
Article
Multidisciplinary Sciences
Kelvin Agboh, Calvin H. F. Lau, Yvonne S. K. Khoo, Himansha Singh, Sagar Raturi, Asha Nair, Julie Howard, Marco Chiapello, Renata Feret, Michael J. Deery, Satoshi Murakami, Hendrik W. van Veen
Article
Infectious Diseases
Calvin Ho-Fung Lau, Erica N. DeJong, Forest Dussault, Catherine Carrillo, Peter J. Stogios, Alexei Savchenko, Edward Topp
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2020)
Article
Environmental Sciences
Calvin Ho-Fung Lau, Yuan-Ching Tien, Robert D. Stedtfeld, Edward Topp
SCIENCE OF THE TOTAL ENVIRONMENT
(2020)
Article
Environmental Sciences
Jessica Subirats, Roger Murray, Andrew Scott, Calvin Ho-Fung Lau, Edward Topp
SCIENCE OF THE TOTAL ENVIRONMENT
(2020)
Article
Biotechnology & Applied Microbiology
Liam P. Brown, Roger Murray, Andrew Scott, Yuan-Ching Tien, Calvin Ho-Fung Lau, Vera Tai, Edward Topp
Summary: This study evaluated the effects of long-term and repeated exposure to macrolide antibiotics on the diversity of soil bacterial community, resistome, and mobilome. At unrealistically high concentrations, macrolide antibiotics altered the overall diversity of resistome and mobilome, enriching for antibiotic resistance genes and mobile genetic elements of concern to human health. However, at realistic antibiotic concentrations, no effect on these endpoints was observed, suggesting that current biosolids land management practices are unlikely to pose a risk to human health due to macrolide antibiotic contamination alone.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Microbiology
Chongwu Yang, Quail Das, Muhammad A. A. Rehman, Xianhua Yin, Julie Shay, Martin Gauthier, Calvin Ho-Fung Lau, Kelly Ross, Moussa S. S. Diarra
Summary: This study investigated the impact of coccidiosis vaccination and dietary berry pomaces on the cecal microbiome of broiler chickens. The results showed that vaccinated birds had a lower abundance of Lactobacillus and a higher abundance of Escherichia coli compared to non-vaccinated birds. The addition of American cranberry or lowbush blueberry pomace to the diet increased the abundance of Lactobacillus and decreased the abundance of Escherichia coli. Overall, coccidiosis vaccination and dietary berry pomaces significantly affected the cecal microbiota, virulome, resistome, and metabolic pathways in broiler chickens.
Article
Genetics & Heredity
Julie A. A. Shay, Laura S. E. Haniford, Ashley Cooper, Catherine D. D. Carrillo, Burton W. W. Blais, Calvin Ho-Fung Lau
Summary: This article describes a targeted metagenomic sequencing method for evaluating the resistome profile of complex food or environmental samples. The study found that retail foods could carry diverse resistance-conferring genes, indicating a potential impact on the dissemination of antimicrobial resistance.
ENVIRONMENTAL MICROBIOME
(2023)
Article
Biotechnology & Applied Microbiology
Calvin Ho-Fung Lau, Kalene van Engelen, Stephen Gordon, Justin Renaud, Edward Topp
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2017)