Article
Medicine, Research & Experimental
Mei-Chun Zeng, Qing-Jun Jia, Lei-Ming Tang
Summary: The study aimed to analyze genetic mutations in rpoB gene of rifampin-resistant Mycobacterium tuberculosis isolates from Zhejiang, China. The most frequent mutation was S450L, and mutations associated with poly-drug resistant, pre-XDR, and XDR TB were mainly located at codon 445 or 450 in the RRDR. Further studies are needed to clarify the relationships between RIF(R) and specific mutations.
JOURNAL OF INTERNATIONAL MEDICAL RESEARCH
(2021)
Article
Infectious Diseases
Ma-chao Li, Jie Lu, Yao Lu, Tong-yang Xiao, Hai-can Liu, Shi-qiang Lin, Da Xu, Gui-lian Li, Xiu-qin Zhao, Zhi-guang Liu, Li-li Zhao, Kang-lin Wan
Summary: The study identified 34 mutations within the rpoB gene, with 25 of them altering structural interactions with RIF and contributing to resistance. Specific mutations such as S450L, H445D, H445Y, H445R were associated with high-level resistance, while D435V was linked to moderate resistance.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Infectious Diseases
Ma-Chao Li, Xiao-Yue Wang, Tong-Yang Xiao, Shi-Qiang Lin, Hai-Can Liu, Cheng Qian, Da Xu, Gui-Lian Li, Xiu-Qin Zhao, Zhi-Guang Liu, Li -Li Zhao, Kang-Lin Wan
Summary: This study assessed the relationship between variant rpoB mutations and the degree of rifampin/rifabutin resistance in Mycobacterium tuberculosis. The results revealed that certain mutations were associated with resistance to both drugs, while others were only associated with rifampin resistance. Interestingly, some isolates showed resistance to rifampin but susceptibility to rifabutin.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Infectious Diseases
Yong Kyun Kim, Yewon Eom, Eunsil Kim, Euijin Chang, Seongman Bae, Jiwon Jung, Min Jae Kim, Yong Pil Chong, Sung-Han Kim, Sang-Ho Choi, Sang-Oh Lee, Yang Soo Kim
Summary: This study investigated the prevalence and molecular characteristics of rifampin-resistant (RIF-R) Staphylococcus aureus (S. aureus) blood isolates in South Korea. The results showed that RIF-R S. aureus blood isolates have unique molecular features and are closely associated with rpoB gene mutations.
Article
Microbiology
Ming-Chih Yu, Ching-Sheng Hung, Chun-Kai Huang, Cheng-Hui Wang, Yu-Chih Liang, Jung-Chun Lin
Summary: Drug resistance in Mycobacterium tuberculosis is a global health issue, and traditional methods for detecting resistance are time-consuming and inefficient. In this study, a whole-genome sequencing pipeline using the Nanopore MinION platform was developed to diagnose drug-resistant MTB isolates more accurately and quickly.
MICROBIOLOGY SPECTRUM
(2022)
Review
Microbiology
Ian L. Sparks, Keith M. Derbyshire, William R. Jacobs Jr, Yasu S. Morita
Summary: Mycobacterium smegmatis is a nonpathogenic and fast growing species that has been elevated to model status for mycobacterial research, providing valuable insights into other pathogens within this genus.
JOURNAL OF BACTERIOLOGY
(2023)
Article
Microbiology
Deepika Rai, Sarika Mehra
Summary: Bacteria use active drug efflux across membranes as a major survival strategy against drugs. Overexpression of certain efflux pumps can significantly increase bacterial tolerance to drugs, and this phenomenon can be modulated by altering the expression level of the efflux pump.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Microbiology
Joseph Shea, Tanya A. Halse, Donna Kohlerschmidt, Pascal Lapierre, Herns A. Modestil, Cheryl H. Kearns, Felicia F. Dworkin, Jennifer L. Rakeman, Vincent Escuyer, Kimberlee A. Musser
Summary: Rapid and reliable detection of rifampin resistance is crucial for tuberculosis treatment, but low-level resistance strains may be missed by routine testing, highlighting the importance of whole-genome sequencing in identifying these strains.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Microbiology
Andres Rivera-Velez, Laura Huber, Samiran Sinha, Noah D. Cohen
Summary: The study found that under nutrient-rich conditions, fitness of MDR-R. equi(.erm(51)+,) (rpoB+) is reduced relative to susceptible isolates, but under nutrient-restricted conditions, they grow similarly. This suggests that resistant isolates might be outcompeted by susceptible ones in nature when antimicrobial use is reduced, but could persist in farm environments.
VETERINARY MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Rita Hirmondo, Armin Horvath, Daniel Molnar, Gyorgy Torok, Liem Nguyen, Judit Toth
Summary: This study investigates the association between cell elongation and DNA synthesis in bacteria, and the interconnection between DNA and cell wall biosynthesis via dTTP regulation. The overexpression of RmlA in Mycobacterium smegmatis leads to changes in cell morphology, reduced cellular dTTP pool, and increased gene expression of replication and cell wall biosynthesis stress factors.
Article
Biotechnology & Applied Microbiology
Joss D. Leehan, Wayne L. Nicholson
Summary: The study demonstrated that the spectrum of mutations in the Bacillus subtilis rpoB gene depends on the growth environment, with different mutations dominating in LB and SMMAsn media. Additionally, competition experiments showed that the fitness of the S487L mutant was significantly higher in SMMAsn medium compared to LB medium. The results highlight the impact of growth environment on mutation and fitness of bacterial strains.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Microbiology
Wan-Hsuan Lin, Wei-Ting Lee, Hsing-Yuan Tsai, Ruwen Jou
Summary: Disputed rpoB mutations are not rare and can impact the management of RR and MDR TB cases, suggesting the use of sequencing and/or MIC testing for better management. Studies show statistically significant differences in treatment outcomes between cases treated with active and inactive fluoroquinolones.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Microbiology
R. R. Clark, P. Lapierre, E. Lasek-Nesselquist, T. A. Gray, K. M. Derbyshire
Summary: The study reveals that horizontal gene transfer (HGT) through distributive conjugal transfer (DCT) is active and prevalent among natural isolates of Mycobacterium smegmatis. It identifies a single gene, Msmeg0070, that confers mating compatibility and maps within the esx1 secretion locus, suggesting a kin recognition system similar to other model organisms. The findings highlight the importance of cell-cell communication and kin identification in mycobacterial conjugation.
Article
Multidisciplinary Sciences
Binayak Rimal, Sibusiso Senzani, Christopher Ealand, Gyanu Lamichhane, Bavesh Kana, Sung Joon Kim
Summary: This study determined the chemical composition and molecular architecture of mycobacterial PG using high-resolution liquid chromatography-mass spectrometry. The findings provide important insights into the unique linkages and chemical modifications of mycobacterial PG, suggesting a possible role of PG acetylation in cell wall homeostasis regulation in M. smegmatis.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Artem S. Grigorov, Yulia V. Skvortsova, Oksana S. Bychenko, Leonid V. Aseev, Ludmila S. Koledinskaya, Irina V. Boni, Tatyana L. Azhikina
Summary: This study conducted comparative transcriptomic analysis on cold-tolerant mycobacteria and found distinct gene expression patterns during the acclimation and adaptation phases. The adaptation strategies developed by mycobacteria were found to be unique compared to other bacteria. These findings shed light on the molecular mechanisms underlying the survival of mycobacteria under cold stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Infectious Diseases
Hongsheng Wang, Weijing Liu, Yali Jin, Meiwen Yu, Haiqin Jiang, Toshiki Tamura, Yumi Maeda, Masahiko Makino
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2015)
Article
Immunology
Yumiko Tsukamoto, Yumi Maeda, Toshiki Tamura, Tetsu Mukai, Satoshi Mitarai, Saburo Yamamoto, Masahiko Makino
Article
Infectious Diseases
Yuji Miyamoto, Tetsu Mukai, Masanori Matsuoka, Masanori Kai, Yumi Maeda, Masahiko Makino
PLOS NEGLECTED TROPICAL DISEASES
(2016)
Article
Immunology
Malcolm S. Duthie, Maria T. Pena, Gigi J. Ebenezer, Thomas P. Gillis, Rahul Sharma, Kelly Cunningham, Michael Polydefkis, Yumi Maeda, Masahiko Makino, Richard W. Truman, Steven G. Reed
Article
Infectious Diseases
Hongsheng Wang, Yumi Maeda, Yasuo Fukutomi, Masahiko Makino
BMC INFECTIOUS DISEASES
(2013)
Article
Infectious Diseases
Yumiko Tsukamoto, Yumi Maeda, Toshiki Tamura, Tetsu Mukai, Masahiko Makino
BMC INFECTIOUS DISEASES
(2014)
Article
Immunology
Tetsu Mukai, Yumiko Tsukamoto, Yumi Maeda, Toshiki Tamura, Masahiko Makino
CLINICAL AND VACCINE IMMUNOLOGY
(2014)
Article
Infectious Diseases
Yumiko Tsukamoto, Yumi Maeda, Masahiko Makino
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2014)
Article
Immunology
Malcolm S. Duthie, Rhea N. Coler, John D. Laurance, Lucas H. Sampaio, Regiane M. Oliveira, Ana Lucia M. Sousa, Mariane M. A. Stefani, Yumi Maeda, Masanori Matsuoka, Masahiko Makino, Steven G. Reed
INFECTION AND IMMUNITY
(2014)
Article
Infectious Diseases
M. Kai, N. Nakata, M. Matsuoka, T. Sekizuka, M. Kuroda, M. Makino
INFECTION GENETICS AND EVOLUTION
(2013)
Article
Microbiology
Kazue Nakanaga, Tsuyoshi Sekizuka, Hanako Fukano, Yumi Sakakibara, Fumihiko Takeuchi, Shinpei Wada, Norihisa Ishii, Masahiko Makino, Makoto Kuroda, Yoshihiko Hoshino
JOURNAL OF CLINICAL MICROBIOLOGY
(2014)
Review
Dermatology
Kazue Nakanaga, Yoshihiko Hoshino, Rie R. Yotsu, Masahiko Makino, Norihisa Ishii
JOURNAL OF DERMATOLOGY
(2013)
Article
Multidisciplinary Sciences
Tsuyoshi Sekizuka, Masanori Kai, Kazue Nakanaga, Noboru Nakata, Yuko Kazumi, Shinji Maeda, Masahiko Makino, Yoshihiko Hoshino, Makoto Kuroda
Article
Immunology
Yuji Miyamoto, Yumiko Tsukamoto, Yumi Maeda, Toshiki Tamura, Tetsu Mukai, Manabu Ato, Masahiko Makino
Summary: Researchers developed an antibiotic-resistant gene rBCG called BCG-PEST but found it unsuitable for human use, so they created an antibiotic-resistant gene-free rBCG named BCG-LeuPH. BCG-LeuPH successfully activated human naive T cells and inhibited aerosol-challenged MTB in mice, suggesting a potential replacement for antibiotic-resistant gene in selecting vaccine candidates for rBCG.
Correction
Immunology
Malcolm S. Duthie, Maria T. Pena, Gigi J. Ebenezer, Thomas P. Gillis, Rahul Sharma, Kelly Cunningham, Michael Polydefkis, Yumi Maeda, Masahiko Makino, Richard W. Truman, Steven G. Reed