Article
Agriculture, Multidisciplinary
Hongyan Yang, Lan Wang, Libo Yuan, Heng Du, Boyuan Pan, Kui Lu
Summary: In this study, a series of hybrid peptides were designed by connecting an antimicrobial peptide Ce(1-8) with a lipopolysaccharide-targeting peptide Lf(28-34) via different linkers. The results showed that the linkers played a crucial role in the antimicrobial activity of the hybrid peptides against Gram-negative bacteria. Among these hybrid peptides, peptide CL5 with a dipeptide rigid linker exhibited excellent activity and selectivity against Gram-negative bacteria. It was found that the interaction of the peptides with LPS was crucial for their antimicrobial activity. Mechanistic studies revealed that peptide CL5 disrupted the integrity of bacterial membranes, leading to the death of Gram-negative bacterial cells. This study highlights the importance of linker selection in the design of hybrid peptides and provides a foundation for the development of antimicrobial peptides.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Kunhong Zhong, Yuelong Wang, Zeng Wang, Zongliang Zhang, Shasha Zhao, Hexian Li, Jianhan Huang, Wenhao Guo, Xi Zheng, Gang Guo, Liangxue Zhou, Hui Yang, Aiping Tong
Summary: The study identified a human antimicrobial peptide called AP-64, which displayed significant antibacterial activity against Gram-negative bacteria, effectively combating Escherichia coli O157:H7 infections and exhibiting cytotoxic effects against human lymphoma cells. Additionally, a mouse homologous gene encoded peptide Gm94 showed similar antibacterial properties to AP-64.
Article
Food Science & Technology
Xuefeng Xia, Shiying Song, Shuangyu Zhang, Wei Wang, Junming Zhou, Baochao Fan, Li Li, Hailong Dong, Chuping Luo, Bin Li, Xuehan Zhang
Summary: The combination of thanatin and BF-15a3 exhibited synergistic antibacterial effects against E. coli O157:H7, with minimal adverse effects on eukaryotic cells. This combination enhanced the damage to E. coli membrane and displayed higher bactericidal activity. Furthermore, it effectively reduced the population of E. coli O157:H7 on fresh-cut lettuce and cucumber.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Microbiology
Melanie D. Balhuizen, Albert van Dijk, Jeroen W. A. Jansen, Chris H. A. van de Lest, Edwin J. A. Veldhuizen, Henk P. Haagsman
Summary: Host defense peptides (HDPs) are part of the innate immune system and possess antimicrobial activity. This study found that certain HDPs induce the release of outer membrane vesicles (OMVs) by bacteria. OMVs act as a means for bacteria to dispose of HDP-affected membrane and act as decoys to protect the bacterium from HDPs.
Article
Infectious Diseases
Yijia Han, Yi Zhang, Weiliang Zeng, Zeyu Huang, Haojun Cheng, Jingchun Kong, Chunquan Xu, Mengxin Xu, Tieli Zhou, Jianming Cao
Summary: In this study, a strategy of combining farnesol (FAR) with colistin (COL) to restart COL activity against colistin-resistant (COL-R) Gram-negative bacteria was proposed. The synergistic antibacterial activity of the COL-FAR combination was confirmed in various experiments, including checkerboard assay, time-killing assay, and bacterial cell viability assay. Moreover, COL-FAR prevented biofilm formation and eradicated pre-existing biofilm, and showed low cytotoxicity. In vivo experiments demonstrated that COL-FAR increased survival rate and reduced bacterial load in infected models, implicating its potential effectiveness against COL-R GNB infections.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Fisheries
Benjamin S. Prior, Miles D. Lange, Scott A. Salger, Benjamin J. Reading, Eric Peatman, Benjamin H. Beck
Summary: Fish-derived antimicrobial peptides, particularly piscidins, have demonstrated potent antimicrobial activity against Gram-negative and Gram-positive bacterial species, with class II piscidins showing greater inhibition against Escherichia coli and Flavobacterium columnare biofilms compared to class I piscidins. However, both classes of piscidins were less effective against inhibiting biofilm growth of Aeromonas hydrophila and A. veronii.
JOURNAL OF FISH DISEASES
(2022)
Article
Infectious Diseases
Qi Zhang, Shang Chen, Xiaojia Liu, Wenhan Lin, Kui Zhu
Summary: The combination of marine antibiotic equisetin and colistin shows a strong synergistic effect against Gram-negative bacteria, especially multi-drug resistant strains. Colistin promotes the intracellular accumulation of equisetin, leading to quick bacteria killing. Equisetin also restores colistin activity in an infection model, providing an alternative approach to combat Gram-negative pathogens in clinics.
Article
Chemistry, Medicinal
Eman M. E. Dokla, Nader S. Abutaleb, Sandra N. Milik, Ezzat A. E. A. Kandil, Omar M. Qassem, Yehia Elgammal, Maha Nasr, Martin J. McPhillie, Khaled A. M. Abouzid, Mohamed N. Seleem, Peter Imming, Mai Adel
Summary: Antibiotic-resistant bacteria pose a serious threat to modern medicine and human life. A study has found that compound 25d, a novel benzimidazole derivative, exhibits potent antibacterial activity against tolC-mutant Escherichia coli without significant cytotoxicity to mammalian cells. Formulation of compound 25d as lipidic nanoparticles further enhances its antibacterial activity against Gram-negative bacterial strains.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Aliya Fazal, Sajida Ara, Muhammad Tayab Ishaq, Kalsoom Sughra
Summary: The study focuses on the green synthesis of copper oxide nanoparticles using vegetable peels, with an ecofriendly approach and low fabrication temperature. It also explores the antibacterial efficacy of the nanoparticles, showing a more significant biocidal effect on Gram-negative bacteria compared to Gram-positive bacteria. The research highlights the potential of utilizing biowaste for the production of useful products.
ARABIAN JOURNAL FOR SCIENCE AND ENGINEERING
(2022)
Article
Microbiology
Laurence Van Moll, Jeroen De Smet, Anne Paas, Dorothee Tegtmeier, Andreas Vilcinskas, Paul Cos, Leen Van Campenhout
Summary: This study evaluated the activity of black soldier fly AMPs against human pathogens and a human cell line, and further explored the characteristics of two cecropins. The results showed that these AMPs exhibited high activity against various bacterial strains, rapid onset of action, and membrane-permeabilizing effects. They were also able to prevent biofilm formation in P. aeruginosa.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Shravani S. Bobde, Fahad M. Alsaab, Guangshuan Wang, Monique L. Van Hoek
Summary: Antimicrobial peptides (AMPs), part of the innate immune system, have potential as a novel therapeutic against bacteria due to their quick-acting mechanism of action. The designed PHNX peptides demonstrated higher antibacterial activity against Gram-negative bacteria, presenting a potential therapeutic option for future clinical use.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Sarabjot Kaur, Rushikesh Tambat, Vikas Pathania, Hemraj Nandanwar, Sanjeev Soni
Summary: This study demonstrates that photo-thermally enhanced triangular silver nanoplates have strong antimicrobial efficacy and can eradicate various pathogens in a short period of time, which is significant for a wide range of antibacterial and antifungal applications.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Sofia Combo, Sergio Mendes, Kaare Magne Nielsen, Gabriela Jorge da Silva, Sara Domingues
Summary: Outer membrane vesicles (OMV) released from Gram-negative bacteria can carry molecules with antibacterial activity, making them potential tools for delivering antimicrobial agents.
Article
Infectious Diseases
Marie Kempf, Francis F. Arhin, Gregory Stone, Eric Utt
Summary: This study assessed the antimicrobial activity of ceftazidime-avibactam (CAZ-AVI) against various bacterial isolates, including Enterobacter spp., Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa. The results demonstrated that CAZ-AVI exhibited potent activity against these isolates, showing high susceptibility rates and low minimum inhibitory concentrations.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2022)
Article
Engineering, Chemical
Yu-Tzu Huang, Selvaraj Rajesh Kumar, Hao-Chun Chan, Zih-Huei Jhan, Dave W. Chen, Shingjiang Jessie Lue
Summary: Bacterial drug resistance is a major global health concern, and certain cationic antimicrobial peptides have been found to be effective bactericidal agents against Escherichia coli, possibly due to interactions leading to collapsed cell walls and pore formation on the bacterial surface.
JOURNAL OF THE TAIWAN INSTITUTE OF CHEMICAL ENGINEERS
(2021)
Article
Clinical Neurology
Tamas Fulop, Usma Munawara, Anis Larbi, Mathieu Desroches, Serafim Rodrigues, Michele Catanzaro, Andrea Guidolin, Abdelouahed Khalil, Francois Bernier, Annelise E. Barron, Katsuiku Hirokawa, Pascale B. Beauregard, David Dumoulin, Jean-Philippe Bellenger, Jacek M. Witkowski, Eric Frost
Article
Chemistry, Medicinal
Gill Diamond, Natalia Molchanova, Claudine Herlan, John A. Fortkort, Jennifer S. Lin, Erika Figgins, Nathen Bopp, Lisa K. Ryan, Donghoon Chung, Robert Scott Adcock, Michael Sherman, Annelise E. Barron
Summary: Antimicrobial peptides (AMPs) have shown potential as novel antiviral drugs by inactivating viruses through disrupting their envelopes. However, their clinical development has been hindered by their enzymatically labile structure. Peptoid mimics of AMPs have been investigated for their potent in vitro antiviral activity against HSV-1 and SARS-CoV-2, with potential as safe and effective broad-spectrum antiviral agents due to their stability and bioavailability.
Article
Geriatrics & Gerontology
Usma Munawara, Michael Catanzaro, Weili Xu, Crystal Tan, Katsuiku Hirokawa, Nabil Bosco, David Dumoulin, Abdelouahed Khalil, Anis Larbi, Simon Levesque, Charles Ramassamy, Annelise E. Barron, Stephen Cunnane, Pascale B. Beauregard, Jean-Pierre Bellenger, Serafim Rodrigues, Mathieu Desroches, Jacek M. Witkowski, Benoit Laurent, Eric H. Frost, Tamas Fulop
Summary: The study found distinct phenotypic and functional changes in monocyte and macrophage populations as the disease progresses. Higher free radical production was observed in the monocytes of SMC patients, while the strongest activation of peripheral monocytes was seen in the MCI group. Monocytes exhibited increased chemotaxis, free radical production, and cytokine production in response to TLR2 and TLR4 stimulation, suggesting activation of the peripheral innate immune system during disease progression.
Article
Chemistry, Medicinal
Josefine Eilso Nielsen, Morgan Ashley Alford, Deborah Bow Yue Yung, Natalia Molchanova, John A. Fortkort, Jennifer S. Lin, Gill Diamond, Robert E. W. Hancock, Havard Jenssen, Daniel Pletzer, Reidar Lund, Annelise E. Barron
Summary: Antimicrobial peptides (AMPs) show promise as pharmaceutical candidates for preventing and treating infections caused by multidrug-resistant pathogens. Peptoids, designed as AMP mimics, exhibit antibacterial activity through self-assembly, with different morphologies affecting efficacy.
ACS INFECTIOUS DISEASES
(2022)
Article
Multidisciplinary Sciences
Leiping Zeng, Yanxia Liu, Xammy Huu Nguyenla, Timothy R. Abbott, Mengting Han, Yanyu Zhu, Augustine Chemparathy, Xueqiu Lin, Xinyi Chen, Haifeng Wang, Draven A. Rane, Jordan M. Spatz, Saket Jain, Arjun Rustagi, Benjamin Pinsky, Adrianna E. Zepeda, Anastasia P. Kadina, John A. Walker, Kevin Holden, Nigel Temperton, Jennifer R. Cochran, Annelise E. Barron, Michael D. Connolly, Catherine A. Blish, David B. Lewis, Sarah A. Stanley, Marie F. La Russa, Lei S. Qi
Summary: The authors demonstrate that CRISPR-Cas13d can effectively inhibit a wide range of human coronaviruses, including new SARS-CoV-2 variants, in combination with small molecule drugs. The CRISPR-Cas13d system reduces viral titers by over 99% and enhances the therapeutic effects of different antiviral drugs. Lipid nanoparticle-mediated RNA delivery enables the Cas13d system to treat infections from multiple coronavirus variants in human primary airway epithelium cultures. This study establishes CRISPR-Cas13 as a broad-spectrum antiviral strategy that complements existing vaccination and antiviral treatment strategies.
NATURE COMMUNICATIONS
(2022)
Article
Immunology
Karim M. Aloul, Josefine Eilso Nielsen, Erwin B. Defensor, Jennifer S. Lin, John A. Fortkort, Mehrdad Shamloo, Jeffrey D. Cirillo, Adrian F. Gombart, Annelise E. Barron
Summary: COVID-19 is characterized by an intense inflammatory response, and this paper reviews the relevance of the human cathelicidin LL-37 in SARS-CoV-2 infections. LL-37 is an immunomodulatory peptide with direct anti-SARS-CoV-2 activity and pleiotropic effects on inflammation, vascularization, Lewy body formation, and pancreatic islet cell function. Upregulation of LL-37 might be beneficial in reducing the prevalence of severe COVID-19.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Microbiology
Aaron B. Benjamin, Madeleine G. Moule, Maruti K. Didwania, Jonathan Hardy, Panatda Saenkham-Huntsinger, Preeti Sule, Josefine Eilso Nielsen, Jennifer S. Lin, Christopher H. Contag, Annelise E. Barron, Jeffrey D. Cirillo
Summary: Staphylococcus aureus resistance is a major concern in healthcare, and antimicrobial peptoids show promise as an alternative to conventional antibiotics for treating S. aureus wound infections, with efficient killing of bacteria and prevention of biofilm formation and detachment.
MICROBIOLOGY SPECTRUM
(2022)
Article
Chemistry, Multidisciplinary
Wenhan Zhao, Haoyu Wang, Xixi Xiao, Lauren De Stefano, Jordan Katz, Jennifer S. Lin, Annelise E. Barron, Thomas P. Schaer, Hongjun Wang, Matthew Libera
Summary: This study investigates the contamination of implantable medical devices by bacteria in the atmosphere of an operating room. The researchers successfully modify titanium surfaces with antimicrobial polymers, which significantly enhance the resistance of the devices to bacterial contamination.
ADVANCED MATERIALS INTERFACES
(2022)
Article
Chemistry, Medicinal
Jennifer S. Lin, Laurent A. Bekale, Natalia Molchanova, Josefine Eilso Nielsen, Megan Wright, Brian Bacacao, Gill Diamond, Havard Jenssen, Peter L. Santa Maria, Annelise E. Barron
Summary: This study aims to explore a new class of anti-persister drugs called peptoids and demonstrates that a specific antimicrobial peptoid can effectively eliminate persister cells and biofilms, as well as inhibit cytokine production induced by lipopolysaccharides.
ACS INFECTIOUS DISEASES
(2022)
Article
Biochemistry & Molecular Biology
Vivek Kumar, Jennifer S. Lin, Natalia Molchanova, John A. Fortkort, Carolin Reckmann, Stefan Braese, Havard Jenssen, Annelise E. Barron, Archana Chugh
Summary: Visceral leishmaniasis (VL) is a neglected tropical disease and the use of cell-permeating peptides as potential drug candidates to treat VL is limited due to their limited biological stability. In this study, peptoids, which are peptide mimics with good biostability, were tested against Leishmania donovani. The addition of N-alkyl hydrophobic chains and bromination yielded compounds with good antileishmanial activity against both intracellular amastigotes and promastigotes, indicating the potential of these peptoids as drug candidates for VL.
Article
Neurosciences
Shirin Shamloo, Erwin Defensor, Peter Ciari, Gaku Ogawa, Laura Vidano, Jennifer S. Lin, John A. Fortkort, Mehrdad Shamloo, Annelise E. Barron
Summary: Photobiomodulation (PBM) is a safe therapeutic approach using specific wavelengths of light to prevent or limit neuroinflammatory processes and neuronal degeneration. In a study on mice, PBM was found to have anti-inflammatory effects by downregulating proinflammatory cytokines and upregulating anti-inflammatory cytokines, resulting in reducing LPS-induced peripheral and central inflammation. This study demonstrates the potential of PBM as an effective anti-inflammatory treatment.
FRONTIERS IN NEUROSCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Minsang Kim, Yeongmi Cheon, Dongmin Shin, Jieun Choi, Josefine Eilso Nielsen, Myeong Seon Jeong, Ho Yeon Nam, Sung-Hak Kim, Reidar Lund, Havard Jenssen, Annelise E. Barron, Seongsoo Lee, Jiwon Seo
Summary: Antimicrobial peptides (AMPs) are effective against multidrug-resistant bacteria, and peptoids with N-substituted glycine backbone have also shown promise as antimicrobials. This study investigates the structure-activity relationship (SAR) of a library of peptoids and demonstrates that both membrane disruption and intracellular biomass flocculation are important mechanisms of bacterial killing.
Article
Chemistry, Medicinal
Patrick M. Tate, Vincent Mastrodomenico, Christina Cunha, Joshua McClure, Annelise E. Barron, Gill Diamond, Bryan C. Mounce, Kent Kirshenbaum
Summary: The development of durable new antiviral therapies is challenging due to rapid virus evolution and resistance. Peptoids, a family of AMP mimetics, show direct antiviral activity against enveloped viruses by disrupting viral membrane constituents. These findings suggest that peptoids have the potential to be a new family of therapeutics capable of preventing resistance.
ACS INFECTIOUS DISEASES
(2023)
Meeting Abstract
Biochemistry & Molecular Biology
Minsang Kim, Yeongmi Cheon, Dongmin Shin, Jieun Choi, Josefine Eilso Nielsen, Myeong Seon Jeong, Reidar Lund, Havard Jenssen, Annelise E. Barron, Seongsoo Lee, Jiwon Seo
JOURNAL OF PEPTIDE SCIENCE
(2022)
Review
Clinical Neurology
Tamas Fulop, Shreyansh Tripathi, Serafim Rodrigues, Mathieu Desroches, Ton Bunt, Arnold Eiser, Francois Bernier, Pascale B. Beauregard, Annelise E. Barron, Abdelouahed Khalil, Adam Plotka, Katsuiku Hirokawa, Anis Larbi, Christian Bocti, Benoit Laurent, Eric H. Frost, Jacek M. Witkowski
Summary: Alzheimer's disease is the most common form of dementia, with aging being the main risk factor. The exact cause of AD is unknown, but research suggests that chronic low-grade infection in the brain may play a role in the development of the disease. Inflammation may lead to neurodegeneration in AD.
NEUROPSYCHIATRIC DISEASE AND TREATMENT
(2021)
