Journal
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
Volume 56, Issue 2, Pages 1116-1119Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AAC.00480-11
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Funding
- INSERM [U914]
- Ministere de l'Education Nationale et de la Recherche [UPRES-EA3539]
- Universite Paris XI, France
- European Community [TROCAR [HEALTH-F3-2008-223031], TEMPOtest-QC [HEALTH-2009-241742]]
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Broad-host-range plasmid pRIO-5, harboring the extended-spectrum beta-lactamase bla(BES-1) gene in Serratia marcescens, was fully sequenced. Analysis of the 12,957-bp sequence of this IncP6-type plasmid revealed that the bla(BES-1) gene was associated with two copies of the insertion sequence IS26. The promoter responsible for the bla(BES-1) expression was hybrid, made of a - 35 box located inside the inverted repeat of IS26 and a - 10 box inside a remnant of an insertion sequence.
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