Article
Biochemistry & Molecular Biology
Abhishek Kumar Verma, Sk Faisal Ahmed, Md Shahadat Hossain, Ali Asger Bhojiya, Ankita Mathur, Sudhir K. Upadhyay, Abhishek K. Srivastava, Naveen Kumar Vishvakarma, Mayadhar Barik, Md Mizanur Rahaman, Newaz Mohammed Bahadur
Summary: This study aimed to find the best candidate drugs as inhibitors of Penicillin Binding Protein-2a for Methicillin-resistant Staphylococcus aureus (MRSA). The results showed that natural flavonoids have potential as adjuvant antimicrobial therapy against resistant strains, demonstrating good interactions in molecular docking and molecular dynamics simulations.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Infectious Diseases
Yan Chen, Lu Sun, Xiaoliang Ba, Shengnan Jiang, Hemu Zhuang, Feiteng Zhu, Haiping Wang, Peng Lan, Qiucheng Shi, Zhengan Wang, Yiyi Chen, Keren Shi, Shujuan Ji, Yan Jiang, Mark A. Holmes, Yunsong Yu
Summary: This study investigated the genomic epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) in China and found a high prevalence of the CC59 lineage in community-associated MRSA. Almost all isolates in the CC59 lineage showed genetic variants leading to increased beta-lactam susceptibility, suggesting potential for improved antibiotic stewardship.
CLINICAL MICROBIOLOGY AND INFECTION
(2022)
Article
Chemistry, Medicinal
Dingyi Shen, Songpo Duan, Yuhan Hu, Jintong Liang, Youzhi Tang
Summary: This study found that the phloroglucinol derivative TPG and 2-nitrophloroglucinol exhibited potent synergistic antibacterial activity with penicillin or bacitracin against MRSA. The combination disrupted the MRSA cell membrane and inhibited the expression of related proteins, effectively inhibiting MRSA growth.
PHYTOTHERAPY RESEARCH
(2023)
Article
Pharmacology & Pharmacy
Mohadeseh Mohammadi Tabar, Moj Khaleghi, Elham Bidram, Atefeh Zarepour, Ali Zarrabi
Summary: Infectious diseases are the second leading cause of death worldwide, and nanotechnology provides promising approaches to tackle antibiotic resistance. This study synthesized a carbon-based nanoplatform and successfully loaded two ineffective antibiotics to enhance their antibacterial activity.
Article
Microbiology
Selvi C. Ersoy, Henry F. Chambers, Richard A. Proctor, Adriana E. Rosato, Nagendra N. Mishra, Yan Q. Xiong, Arnold S. Bayer
Summary: The study highlights the distinct regulatory impact of NaHCO3 on a cadre of genes encoding factors required for the maintenance of the MRSA phenotype through PBP2a functionality and maturation. NaHCO3 exposure led to reduced expression of mecA, blaZ, the vraSR-prsA gene axis, and pbp4, while increasing the expression of floA in all MRSA strains. Additionally, carotenoid production was decreased in NaHCO3-responsive MRSA strains.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Microbiology
Selvi C. Ersoy, Adhar C. Manna, Richard A. Proctor, Henry F. Chambers, Ewan M. Harrison, Arnold S. Bayer, Ambrose Cheung
Summary: The study aimed to investigate the relationship between specific mecA genotypes and PBP2a substitutions with NaHCO3-responsiveness. The results demonstrated that NaHCO3-responsiveness is not only determined by the mecA genotype but also depends on the strain-specific genetic background.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Review
Materials Science, Multidisciplinary
Shreya Kanth, Akshatha Nagaraja, Yashoda Malgar Puttaiahgowda
Summary: The current global death rate is threatened by deadly unknown infections and the emergence of multidrug-resistant bacteria. Combatting drug-resistant bacteria, such as MRSA, has become a daunting challenge globally, with polymer synthesis being explored as a significant approach in preventing the spread of infections.
JOURNAL OF MATERIALS SCIENCE
(2021)
Article
Cell Biology
Zhimin Bai, Min Chen, Qiaofa Lin, Ying Ye, Hongmei Fan, Kaizhen Wen, Jianxing Zeng, Donghong Huang, Wenfei Mo, Ying Lei, Zhijun Liao
Summary: By extracting feature vectors from protein sequences and using classification tools, MRSA and MSSA were successfully distinguished. MRSA showed high resistance to penicillin and high pathogenic risk. Cross-infection between different types of MRSA was found in Quanzhou, and MRSA in traditional hospitals exhibited increasingly blurred molecular characteristics.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Infectious Diseases
Wenxu Han, Terri A. Camesano
Summary: Methicillin-resistant Staphylococcus aureus (MRSA) infections pose a serious threat to public health. This study investigated the potential of antimicrobial peptides (AMPs) LL37, FK16, and FK13 to enhance the activity of antibiotics against MRSA in vitro, specifically penicillin G and ampicillin. The results showed that FK16 and FK13 had synergistic inhibitory effects on MRSA strains when combined with penicillin G and ampicillin. AMPs also exhibited membrane permeabilizing properties and electrostatic interactions with MRSA. FK16 showed the most promising results, reducing the minimum inhibitory concentrations (MICs) of both penicillin G and ampicillin for all MRSA strains and demonstrating greater synergistic effects with antibiotics. AMP exposure and membrane permeabilization provide a potential pathway to re-sensitize drug-resistant bacteria to traditional antibiotics.
Review
Infectious Diseases
Joshua. B. Parsons, Annette. C. Westgeest, Brian. P. B. Conlon, Vance. G. Fowler
Summary: Methicillin-resistant Staphylococcus aureus (MRSA) is a deadly pathogen that can persist in the bloodstream for days despite appropriate antibiotics. Persistent MRSA bacteremia is common and associated with poor clinical outcomes. This review explores the factors related to host-pathogen interaction and discusses the clinical relevance of each element. Treatment options and diagnostic approaches for managing persistent MRSA bacteremia are also discussed.
Article
Public, Environmental & Occupational Health
Feiteng Zhu, Hemu Zhuang, Shujuan Ji, Er Xu, Lingfang Di, Zhengan Wang, Shengnan Jiang, Haiping Wang, Lu Sun, Ping Shen, Yunsong Yu, Yan Chen
Summary: The study found that in households of CA-MRSA infected patients in China, most MRSA isolates belonged to epidemic CA-MRSA clones, while no MRSA was detected in the control group households. The research revealed that the home environment is an important reservoir for MRSA.
FRONTIERS IN PUBLIC HEALTH
(2021)
Article
Microbiology
Melissa J. Karau, Suzannah M. Schmidt-Malan, Scott A. Cunningham, Jayawant N. Mandrekar, Bobbi S. Pritt, Tiffany R. Keepers, Alisa W. Serio, Surya Chitra, Robin Patel
Summary: Omadacycline, vancomycin, and rifampin, as well as rifampin combination therapies, were evaluated in an experimental rat model of MRSA osteomyelitis. The results showed that rifampin combination therapy was effective in reducing MRSA recovery without developing resistance. The combination of omadacycline and rifampin yielded negative MRSA detection, suggesting its potential as a treatment for osteomyelitis.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Microbiology
Sayed Golam Mohiuddin, Sreyashi Ghosh, Pouria Kavousi, Mehmet A. Orman
Summary: Methicillin-resistant Staphylococcus aureus (MRSA) is a dangerous pathogen that is resistant to conventional antibiotics. This study found that proton motive force (PMF) inhibitors have bactericidal effects on MRSA cells by disrupting PMF and permeabilizing cell membranes. By screening a chemical library, specific drugs that can disrupt MRSA cell PMF and reduce cell levels were identified. This highlights the importance of targeting cellular PMF for developing new bactericidal therapeutics for pathogens.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Jian Peng, Biswajit Mishra, Rajamohammed Khader, LewisOscar Felix, Eleftherios Mylonakis
Summary: In this study, a smaller antimicrobial peptide C18 derived from cecropin 4 showed high antibacterial activity against various bacteria and yeasts, with a remarkable effect on drug-resistant Staphylococcus aureus. C18 also exhibited the capability to eliminate persister cells, reduce biofilm adherence, interact with DNA, and down-regulate virulence factor genes. Moreover, C18 showed promising results in a Galleria mellonella model, highlighting its potential as a therapeutic candidate for severe MRSA infections.
Article
Food Science & Technology
Martina Florianova, Kristyna Korena, Helena Juricova
Summary: This study evaluated the prevalence of Staphylococcus aureus in dry-fermented salami and found multidrug-resistant and virulent strains. The source of contamination was identified as raw pork and human carriers. Therefore, maintaining high standards of hygiene and manufacturing practices in food processing is crucial.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Harika Vemula, Navid J. Ayon, William G. Gutheil
Article
Biochemical Research Methods
Mohit Jaiswal, Subhradeep Bhar, Harika Vemula, Swami Prakash, V. K. Chaithanya Ponnaluri, William G. Gutheil, Mridul Mukherji
PROTEIN EXPRESSION AND PURIFICATION
(2017)
Article
Cell Biology
Yukiko Kitase, Julian A. Vallejo, William Gutheil, Harika Vemula, Katharina Jaehn, Jianxun Yi, Jingsong Zhou, Marco Brotto, Lynda F. Bonewald
Article
Biochemistry & Molecular Biology
William G. Gutheil
BIOPHYSICAL CHEMISTRY
(2018)
Article
Biochemical Research Methods
Navid J. Ayon, Amar Deep Sharma, William G. Gutheil
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2019)
Article
Multidisciplinary Sciences
Chayan Bhattacharya, Aninda Sundar Dey, Navid J. Ayon, William G. Gutheil, Mridul Mukherji
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2018)
Article
Biochemistry & Molecular Biology
Navid J. Ayon, William G. Gutheil
ACS CHEMICAL BIOLOGY
(2019)
Correction
Biochemistry & Molecular Biology
Navid J. Ayon, William G. Gutheil
ACS CHEMICAL BIOLOGY
(2021)
Article
Microbiology
Shivani Gargvanshi, Harika Vemula, William G. Gutheil
Summary: This study investigated the response of VanA-type VREfm to vancomycin exposure, showing changes in levels of normal and alternative pathway PG intermediates. Normal pathway intermediates responded rapidly, while alternative pathway intermediates had a slower response time.
JOURNAL OF BACTERIOLOGY
(2021)
Article
Microbiology
Shivani Gargvanshi, William G. Gutheil
Summary: This study screened 1,000 FDA-approved drugs and their metabolites against vancomycin-resistant bacteria and identified potentially synergistic combinations with vancomycin. Several drugs were confirmed to have synergistic effects in follow-up assays.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Amar Deep Sharma, William G. Gutheil
Summary: New strategies are urgently needed to address antimicrobial resistance. Synergistic agent combinations provide a potential approach to combating this problem. In this study, a screening of an FDA-approved drug library identified several beta-lactam antibiotics with synergistic effects with ceftobiprole.
MICROBIOLOGY SPECTRUM
(2023)
Article
Chemistry, Multidisciplinary
Buwanila T. Punchihewa, Vidit Minda, William G. Gutheil, Mohammad Rafiee
Summary: Electrochemistry plays a crucial role in chemical reactions and has wide applications in electroanalysis and electrosynthesis. By designing an electrochemical cell with a thin layer electrode, the limitations of traditional electrolysis cells can be bypassed, allowing for minute-scale electrolysis reactions and real-time probing of redox-active components.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Microbiology
Shivani Gargvanshi, Gioia Heravi, Navid J. Ayon, William G. Gutheil
Summary: New antibacterial agents and agent combinations are urgently needed to combat antimicrobial resistance. A multidimensional chemical library screening strategy was used to identify compounds with anti-MRSA activity. The study demonstrated the utility of comparative library screening in identifying novel bioactive agents with interesting synergies and biological activities.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Harika Vemula, Navid J. Ayon, Alloch Burton, William G. Gutheil
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2017)
Article
Biochemical Research Methods
Harika Vemula, Yukiko Kitase, Navid J. Ayon, Lynda Bonewald, William G. Gutheil
ANALYTICAL BIOCHEMISTRY
(2017)