Article
Biochemistry & Molecular Biology
Zhilei Li, Lang He, Xia Wang, Qingwen Huo, Guosong Zheng, Dekun Kong, Yinhua Lu, Haiyang Xia, Guoqing Niu
Summary: Sideromycins are a unique subset of siderophores that combine a siderophore and an antimicrobial agent. This study deciphered the biosynthetic pathway of the ferrichrome-type siderophore in Streptomyces sp. ATCC 700974 and identified the involved genes. The findings provide important insights into the synthesis of albomycin and the role of multiple siderophores in Streptomyces sp. ATCC 700974.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Petra Pivk Lukancic, Tjasa Drcar, Robert Bruccoleri, Martin Crnugelj, Peter Mrak
Summary: In this study, a new genotype of Streptomyces clavuligerus with the ability to produce teleocidin was discovered. Teleocidin is a highly toxic secondary metabolite. The genome sequence of this genotype provides a foundation for further research and application.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Luping Pang, Stephen D. Weeks, Arthur Van Aerschot
Summary: Aminoacyl-tRNA synthetases (aaRSs) are essential enzymes in all three kingdoms of life, and have been recognized as suitable targets for small molecule anti-infectives. This review discusses the catalytic activities of aaRSs, inhibitory mechanisms, and species-selectivity determinants of reported inhibitors. The structural perspective highlights opportunities for further exploration of the aaRS enzyme family as antimicrobial targets.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Wangjie Zhu, Xinwei Pei, Xiaoyu Chen, You Wu, Fuhang Song, Huawei Zhang
Summary: Comparative transcriptome analysis revealed significant differences in chrysomycin biosynthetic gene cluster-related gene expression between mutant 891-B6 and wild-type strain 891, with several genes significantly up-regulated in mutant 891-B6. These findings provide molecular mechanisms for increased CA yield and decreased CB yield in mutant 891-B6.
Article
Chemistry, Multidisciplinary
Zhou-Tian-Le Zhang, Hui-Bing Sun, Zhen Ren, Tian-Peng Xie, Ying-Fang Wang, Yin Guo, Xiaoyu Su, Min Yin, Hao Zhou, Zhong-Tao Ding
Summary: In this study, actinomycins and their derivatives were isolated from endophytic actinomycetes derived from traditional Chinese herbs. The discovery of a shunt pathway in the early stages of actinomycin D biosynthesis provides a fresh perspective for further research and modification of novel actinomycins.
Article
Microbiology
Yi-huang Chen, Yi Zheng Wu, Qin Liu, Zhanfeng Xia, Jianming Wang, Xiao-Xia Luo
Summary: This study focused on the antagonistic strain of Streptomyces against pear black spot disease in Xinjiang. The researchers identified the antibacterial active components of the strain and confirmed its antagonistic activity against Alternaria. The findings are significant for the development of the pear industry and the prevention and control of pear black spot disease in Xinjiang.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Svini Dileepa Marasinghe, Eunyoung Jo, Sachithra Amarin Hettiarachchi, Youngdeuk Lee, Tae-Yang Eom, Yehui Gang, Yoon-Hyeok Kang, Chulhong Oh
Summary: The study focused on the GH11 xylanase gene xynS1 from Streptomyces sp. strain J103, which can mediate protein synthesis and lignocellulosic material hydrolysis. The results showed that rXynS1 exhibited optimal activity under specific conditions, and its combination with other enzymes could enhance xylan degradation.
MICROBIAL CELL FACTORIES
(2021)
Article
Biochemistry & Molecular Biology
Shahar Garin, Ofri Levi, Megan E. Forrest, Anthony Antonellis, Yoav S. Arava
Summary: This study revealed that aminoacyl-tRNA synthetases not only play a role in tRNA charging but also regulate mRNA expression post-transcriptionally, potentially utilizing RNA elements to coordinate mRNA expression based on amino acid availability.
Article
Biotechnology & Applied Microbiology
Munenori Takehara, Masayuki Saimura, Haruka Inaba, Yoshinao Kato, Shogo Muro, Tatsuki Matsunaga, Kazuya Yamanaka
Summary: Polymers of basic amino acids function as polycationic compounds with various biological properties under physiological conditions, such as antimicrobial and antiviral activities, immunopotentiating ability, and DNA-binding activity. The ε-PL and PAP produced by some Streptomyces strains have potential as food preservatives and inhibitors with wide applications in various fields.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Materials Science, Paper & Wood
Imran Ali, Shastah Sultan, Raja Tahir Mahmood, Muhammad Tariq, Zeeshan Shamim, Aamar Mushtaq, Mohammed Asiri
Summary: This study reports the optimization of conditions for alpha-amylase production from Streptomyces sp. MI-1, isolated from soil in Kotli Azad Kashmir. The study identified the optimal pH and temperature for the growth of Streptomyces MI-1 and the amylase activity in the nutrient medium. The characterization of the bacterial amylase showed its optimum temperature and pH, as well as the effects of different metal ions on its activity.
Article
Microbiology
Muthuraj Rudrappa, M. Santosh Kumar, Raju Suresh Kumar, Abdulrahman I. Almansour, Karthikeyan Perumal, Sreenivasa Nayaka
Summary: In this study, a melanin-producing actinomycete strain was isolated from soil and characterized using various techniques. The results demonstrate the significant value of the melanin pigment in industrial and biomedical applications.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Microbiology
Pulak Kumar Maiti, Sukhendu Mandal
Summary: A novel actinobacterium named Streptomyces cupreus sp. nov. was isolated from a soil sample in Kashmir Himalaya, India. Through multiple approaches, including genetic and phenotypic analysis, it was revealed that PSKA01(T) is a unique species within the genus Streptomyces.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Manee Chanama, Chanwit Suriyachadkun, Suchart Chanama
Summary: In this study, a novel actinobacterium called strain SMC 277(T) was isolated from clay soil in a paddy field in Thailand. The strain was characterized using various taxonomic methods and was identified as a member of the genus Streptomyces. Genome sequencing revealed the presence of 35 biosynthetic gene clusters encoding diverse bioactive secondary metabolites, and the strain showed antimicrobial activity against drug-resistant bacteria associated with nosocomial infections. Based on these findings, the strain was proposed as a new species called Streptomyces antimicrobicus.
Article
Microbiology
Valerio Mattei, Andrea Motta, Marco Saracchi, Andrea Kunova, Paolo Cortesi, Cristina Pizzatti, Matias Pasquali
Summary: Streptomycetes are potential candidates for the biological control of Fusarium Head Blight in wheat. Previous studies mainly focused on the application of streptomycetes when the wheat plant is mature and close to flowering. This study tested the effects of seed treatment with Streptomyces sp. DEF39 spores before sowing on the development of FHB symptoms and found that it could partially protect wheat from FHB disease.
Article
Biotechnology & Applied Microbiology
Cheng-Hong Zhang, Huan Qi, Bing Li, Shao-Yong Zhang, Li-Qin Zhang, Ji-Dong Wang
Summary: Two new macrolide metabolites, phthoramycins B (1) and C (2), were isolated from the fermentation broth of Streptomyces sp. HU210. Their structures were determined using spectroscopic techniques. Compounds 1 and 2 exhibited cytotoxic activity against leukemia and lung cancer cells.
JOURNAL OF ANTIBIOTICS
(2022)
Article
Biotechnology & Applied Microbiology
Aditya Kulkarni, Yu Zeng, Wei Zhou, Steven Van Lanen, Weiwen Zhang, Shawn Chen
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2016)
Review
Biotechnology & Applied Microbiology
Shawn Chen, William A. Kinney, Steven Van Lanen
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2017)
Article
Biochemistry & Molecular Biology
Yu Zeng, Aditya Kulkarni, Zhaoyong Yang, Preeti B. Patil, Wei Zhou, Xiuling Chi, Steven Van Lanen, Shawn Chen
ACS CHEMICAL BIOLOGY
(2012)
Article
Microbiology
Yongli Li, Ying-Mi Lai, Yi Lu, Yu-Liang Yang, Shawn Chen
ARCHIVES OF MICROBIOLOGY
(2014)
Article
Biochemistry & Molecular Biology
Jin Young Kim, Bradley A. Carlson, Xue-Ming Xu, Yu Zeng, Shawn Chen, Vadim N. Gladyshev, Byeong Jae Lee, Dolph L. Hatfield
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2011)
Review
Biochemistry & Molecular Biology
Rajat Banerjee, Shawn Chen, Kiley Dare, Marla Gilreath, Mette Praetorius-Ibba, Medha Raina, Noah M. Reynolds, Theresa Rogers, Herve Roy, Srujana S. Yadavalli, Michael Ibba
Article
Microbiology
Preeti B. Patil, Yu Zeng, Tami Coursey, Preston Houston, Iain Miller, Shawn Chen
FEMS MICROBIOLOGY LETTERS
(2010)
Article
Microbiology
Jianjun Qiao, Lei Chen, Yongli Li, Jiangxin Wang, Weiwen Zhang, Shawn Chen
JOURNAL OF BACTERIOLOGY
(2012)
Article
Multidisciplinary Sciences
Zihua Lin, Xiaobo Xu, Sheng Zhao, Xiaohong Yang, Jian Guo, Qun Zhang, Chunmei Jing, Shawn Chen, Yun He
NATURE COMMUNICATIONS
(2018)
Article
Biochemistry & Molecular Biology
Heng Wang, Min Xu, Curtis A. Engelhart, Xi Zhang, Baohua Yan, Miaomiao Pan, Yuanyuan Xu, Shilong Fan, Renhe Liu, Lan Xu, Lan Hua, Dirk Schnappinger, Shawn Chen
Summary: PF-3845 was identified as a potential inhibitor of Mtb PheRS with a novel binding mode, showing inhibitory activity against Mtb H37Rv and engineered strain Mtb pheS-FDAS. Further medicinal chemistry efforts targeting the piperidine-piperazine urea moiety may lead to the discovery of a selective antibacterial lead compound.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Yuhong Yang, Yuanyuan Xu, Yuan Yue, Heng Wang, Yumeng Cui, Miaomiao Pan, Xi Zhang, Lin Zhang, Haitao Li, Min Xu, Yefeng Tang, Shawn Chen
Summary: Indolmycin (IND) is a microbial natural product that selectively inhibits bacterial tryptophanyl-tRNA synthetase (TrpRS). IND shows promising antibacterial activity against Mycobacterium tuberculosis (Mtb), especially the 4''-methylated compound Y-13. The cocrystal structure of Mtb TrpRS complexed with IND and ATP reveals that IND can affect the activity of TrpRS and has synergistic effects with other antibacterial agents, potentially leading to the development of new antibiotics.
ACS CHEMICAL BIOLOGY
(2022)
Article
Chemistry, Medicinal
Heng Wang, Jing Bi, Yuan Zhang, Miaomiao Pan, Qinglong Guo, Genhui Xiao, Yumeng Cui, Song Hu, Chi Kin Chan, Ying Yuan, Takushi Kaneko, Guoliang Zhang, Shawn Chen
Summary: This study identifies linsitinib, a clinical-stage drug originally targeting kinase IGF1R/IR, as a potent inhibitor of the essential glutamine synthase in Mycobacterium tuberculosis. Linsitinib also improves autophagy flux and reduces intracellular growth of Mtb. These findings suggest that targeting ATP-dependent enzymes like glutamine synthase could be a novel approach for anti-TB therapy.
ACS INFECTIOUS DISEASES
(2022)
Article
Biotechnology & Applied Microbiology
Guangsheng Pei, Tao Sun, Shuo Chen, Lei Chen, Weiwen Zhang
BIOTECHNOLOGY FOR BIOFUELS
(2017)