Article
Biochemistry & Molecular Biology
Yazhen Ma, Xinru Cao, Hui Wang, Xingju Song, Dandan Hu
Summary: Tilmicosin and acetylisovaleryltylosin tartrate (ATLL) have potent anti-Toxoplasma activities, inhibiting the growth and proliferation of the parasite. ATLL shows strong inhibitory effects on intracellular parasites, while tilmicosin suppresses extracellular parasites.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Jessica Ramseier, Dennis Imhof, Nicoleta Anghel, Kai Hanggeli, Richard M. Beteck, Vreni Balmer, Luis-Miguel Ortega-Mora, Roberto Sanchez-Sanchez, Ignacio Ferre, Richard K. Haynes, Andrew Hemphill
Summary: This study aimed to investigate the therapeutic effects of quinolone decoquinate (DCQ) and its derivatives on Toxoplasma gondii, revealing that the quinoline-O-carbamate derivative RMB060 has a lower IC50 value and a higher selectivity index, affecting parasite mitochondria. However, in a mouse model, these drugs did not completely prevent the transmission of Toxoplasma gondii.
Article
Biochemistry & Molecular Biology
Yuan Liu, Yaqin Tang, Xing Tang, Mengqi Wu, Shengjie Hou, Xiaohua Liu, Jing Li, Meichun Deng, Shuaiqin Huang, Liping Jiang
Summary: The study identified a novel spider peptide, XYP1, with potent anti-Toxoplasma activity both in vitro and in vivo. XYP1 inhibited the viability, invasion, and proliferation of tachyzoites with low cytotoxicity on human host cells and improved the survival rate of mice infected with T. gondii. Further exploration of the mechanism revealed XYP1 causes membrane perforation, swelling, and disruption of tachyzoites, potentially through differential expression of membrane-associated proteins including HSP29. Overall, XYP1 shows promise as a new drug candidate for toxoplasmosis treatment.
Article
Infectious Diseases
Sultan F. Alnomasy
Summary: The study demonstrated the significant prophylactic effects of ASEO in increasing survival rate and reducing parasite load in mice with acute toxoplasmosis. ASEO also promoted the innate immune system, regulation of pro-inflammatory cytokines, and inhibition of hepatic injury in the infected mice. Further research is needed to fully understand the prophylactic and therapeutic mechanisms of ASEO against Toxoplasma.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Food Science & Technology
Xiaohua Liu, Peng Zhang, Yuan Liu, Jing Li, Dongqian Yang, Zhonghua Liu, Liping Jiang
Summary: Fatty acid chain modification can improve the serum stability and activity of Lycosin-I, making it a potential novel drug candidate for anti-T. gondii.
Article
Chemistry, Inorganic & Nuclear
Harley D. Betts, Yih Ching Ong, Nicoleta Anghel, Sarah Keller, Johannes Karges, Niovi Voutsara, Joachim Muller, Eric Manoury, Olivier Blacque, Kevin Cariou, Andrew Hemphill, Gilles Gasser
Summary: This study reports the synthesis, characterization, and biological testing of some organometallic derivatives of the antiparasitic drug decoquinate for the treatment of toxoplasmosis. The derivatives reduced the proliferation of the parasite, with ferrocenylvinyl-DCQ and an oxygen-alkylated phenylvinyl-DCQ side product showing similar inhibitory effects as the parent drug.
Article
Parasitology
Dawei Wang, Mengen Xing, Saeed El-Ashram, Yingying Ding, Xiao Zhang, Xiaoyu Sang, Ying Feng, Ran Chen, Xinyi Wang, Ning Jiang, Qijun Chen, Na Yang
Summary: The study found that lumefantrine inhibits the proliferation of T. gondii in Vero cells without being toxic. In mice, lumefantrine at a concentration of 50 μg L-1 prolonged survival by 3 days and reduced parasite burden in tissues. High-dose lumefantrine increased IFN-γ production, while low-dose lumefantrine increased IL-10 and IL-4 levels in mice.
Article
Chemistry, Applied
Ya-Lan Wang, Li-Li Jin, Xu Cheng, Wei-Feng Yan, Hao Deng, Qing-Kun Shen, Zhe-Shan Quan, Chun-Mei Jin, Chang-Hao Zhang
Summary: The derivatives of ursolic acid (UA) were synthesized and tested for their anti-Toxoplasma gondii activity. Compound A7 showed the best activity, surpassing both UA and the positive control drug Spiramycin. Further research revealed that A7 had an impact on the inhibition rate of tachyzoites in mice and caused some damage to the liver. These findings suggest that A7 could be a potential lead compound for developing a novel anti-Toxoplasma gondii molecule.
NATURAL PRODUCT RESEARCH
(2023)
Article
Microbiology
Daisuke Oka, Naomitsu Yamaya, Takuya Kuno, Yuta Asakawa, Toshiyuki Shiragiku, Liang Chen, Jingbo Xue, Abudusaimi Mamuti, Fangguo Ye, Jiangqin Sun, Kinue Ohguro, Hisashi Miyamoto, Yukitaka Uematsu, Katsuya Inagaki, Jie-Fei Cheng, Makoto Matsumoto
Summary: OPS-2071 is a novel quinolone antibacterial agent with potent activity against Clostridioides difficile, exhibiting low systemic exposure and in vivo efficacy, making it a potential new therapeutic option for treating C. difficile infections.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Chemistry, Organic
Ashish C. Kavale, Amit H. Kalbandhe, Imran A. Opai, Atul A. Jichkar, Nandkishor N. Karade
Summary: The oxidation of tertiary alcohols of the type 3-hydroxy-3-phenacyloxindoles using phenyliodine diacetate and molecular iodine in methanol results in the formation of 2-hydroxy-2-aryl/alkyl-2,3-dihydroquinolin-4(1H)-one derivatives in good yields.
TETRAHEDRON LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Yihan Wu, Xiao Zhang, Yong Fu, Jing Liu, Yangfei Xue, Qun Liu
Summary: Progesterone inhibits the invasion and proliferation of toxoplasma gondii, leading to abnormal cytoskeletal division and autophagy. The discovery of the progesterone membrane receptor protein (TgPGRMC) establishes a direct relationship between progesterone and T. gondii and provides a new direction for research on T. gondii infection during pregnancy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Isabela S. Macedo, Flavio A. Lara, Helene S. Barbosa, Elvira M. Saraiva, Rubem F. S. Menna-Barreto, Rafael M. Mariante
Summary: Toxoplasma gondii is a prevalent pathogen that causes toxoplasmosis worldwide. This study investigates the interplay between human neutrophils and T. gondii, exploring the mechanisms and significance of neutrophil extracellular traps (NETs) in infection control. It reveals the formation mechanisms of NETs and their impact on T. gondii infection dynamics, contributing to broader insights into host-pathogen relationships.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Qian-qian Hua, Xue-jing Lin, Shi-peng Xiang, Li-ya Jiang, Jin-hao Cai, Jian-min Sun, Feng Tan, Ya-ni Mou
Summary: Two compounds, CGI-1746 and JH-II-127, showed inhibitory effects on Toxoplasma gondii. CGI-1746 inhibited the invasion, egress, and gliding abilities of the parasites, while JH-II-127 damaged the morphology of mitochondria. These findings lay the groundwork for future therapeutic strategies against T. gondii.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Kyung-Hwa Baek, Trong-Nhat Phan, Satish R. Malwal, Hyeryon Lee, Zhu-Hong Li, Silvia N. J. Moreno, Eric Oldfield, Joo Hwan No
Summary: SQ109 is an anti-tubercular drug candidate that has shown potent activity against protozoan parasites including Leishmania, Trypanosoma cruzi, and Toxoplasma gondii in vitro. It has demonstrated efficacy in mouse models of T. cruzi and T. gondii infections, but moderate efficacy in models of Trypanosoma brucei and Leishmania donovani infections. Metabolites of SQ109 also exhibit activity against protozoan parasites in vitro.
Article
Veterinary Sciences
Mercedes Fernandez-Escobar, Rafael Calero-Bernal, Javier Regidor-Cerrillo, Raquel Vallejo, Julio Benavides, Esther Collantes-Fernandez, Luis Miguel Ortega-Mora
Summary: The study identified significant intra- and inter-genotype virulence differences in Spanish T. gondii isolates, which were not reflected by the genetic characterization using currently described molecular markers. Isolates TgShSp16 (#3) and TgShSp24 (#2) presented higher degrees of virulence, and there were significant differences in invasion rates and tachyzoite yield at 72 h post-inoculation between isolates.
VETERINARY RESEARCH
(2021)
Article
Biotechnology & Applied Microbiology
Uwe Gross, Elzbieta Brzuszkiewicz, Katrin Gunka, Jessica Starke, Thomas Riedel, Boyke Bunk, Cathrin Sproeer, Daniela Wetzel, Anja Poehlein, Cynthia Chibani, Wolfgang Bohne, Joerg Overmann, Ortrud Zimmermann, Rolf Daniel, Heiko Liesegang
Article
Microbiology
Anastasia-Lisa Luebke, Sabrina Minatelli, Thomas Riedel, Raimond Lugert, Isabel Schober, Cathrin Sproeer, Joerg Overmann, Uwe Gross, Andreas E. Zautner, Wolfgang Bohne
Article
Infectious Diseases
Julian Schwanbeck, Thomas Riedel, Friederike Laukien, Isabel Schober, Ines Oehmig, Ortrud Zimmermann, Joerg Overmann, Uwe Gross, Andreas E. Zautner, Wolfgang Bohne
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2019)
Article
Biochemical Research Methods
Wycliffe O. Masanta, Andreas E. Zautner, Raimond Lugert, Wolfgang Bohne, Uwe Gross, Andreas Leha, Mohammed Dakna, Christof Lenz
PROTEOMICS CLINICAL APPLICATIONS
(2019)
Article
Gastroenterology & Hepatology
Hyasinta Jaka, Nele Ruettgerodt, Wolfgang Bohne, Andreas Mueller, Uwe Gross, Christa Kasang, Stephen E. Mshana
CANADIAN JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
(2019)
Article
Microbiology
Matthias F. Emele, Felix M. Joppe, Thomas Riedel, Joerg Overmann, Maja Rupnik, Paul Cooper, R. Lia Kusumawati, Fabian K. Berger, Friederike Laukien, Ortrud Zimmermann, Wolfgang Bohne, Uwe Gross, Oliver Bader, Andreas E. Zautner
FRONTIERS IN MICROBIOLOGY
(2019)
Article
Biochemical Research Methods
Julian Schwanbeck, Ines Oehmig, Jerome Dretzke, Andreas E. Zautner, Uwe Gross, Wolfgang Bohne
BMC BIOINFORMATICS
(2020)
Article
Infectious Diseases
Friederike K. M. T. Tilkorn, Hagen Frickmann, Isabel S. Simon, Julian Schwanbeck, Sebastian Horn, Ortrud Zimmermann, Uwe Gross, Wolfgang Bohne, Andreas E. Zautner
Article
Microbiology
Julian Schwanbeck, Wolfgang Bohne, Ufuk Hasdemir, Uwe Gross, Yvonne Pfeifer, Boyke Bunk, Thomas Riedel, Cathrin Sproeer, Jorg Overmann, Hagen Frickmann, Andreas E. Zautner
Summary: This study investigated the plasmid-resistome of seven bla(OXA-48) gene-carrying Klebsiella pneumoniae isolates in Germany, identifying different phenotypic resistance patterns associated with IncL/M and IncR plasmids among isolates of the ST101 cluster, indicating possible nosocomial transmission.
Article
Microbiology
Julian Schwanbeck, Ines Oehmig, Uwe Gross, Andreas E. Zautner, Wolfgang Bohne
Summary: The translation discusses the importance of flagellar motility for intestinal pathogens and highlights the swimming strategy of Clostridioides difficile at the single cell level and its dependence on environmental parameters.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Medicine, General & Internal
Sascha Dierks, Oliver Bader, Julian Schwanbeck, Uwe Gross, Michael S. Weig, Kemal Mese, Raimond Lugert, Wolfgang Bohne, Andreas Hahn, Nicolas Feltgen, Setare Torkieh, Fenja R. Denker, Peer Lauermann, Marcus W. Storch, Hagen Frickmann, Andreas Erich Zautner
Summary: This study compared the performance characteristics of two SARS-CoV-2-specific rapid antigen assays, as well as a transcription-mediated amplification (TMA) assay and real-time PCR. The results indicated that LumiraDx had higher sensitivity and specificity compared to NADAL antigen assay, and TMA testing was as accurate as PCR in a low prevalence setting.
JOURNAL OF CLINICAL MEDICINE
(2021)
Review
Microbiology
Ortrud Zimmermann, Heinrich Koechel, Wolfgang Bohne, Beatrix Pollok-Kopp, Peter Passenberg, Uwe Gross
Summary: Clostridioides difficile infection (CDI) can also lead to reactive arthritis, characterized by diarrhea or colitis after taking antibiotics, detectable toxigenic C. difficile or only the toxins in stool, and no other explanations for the arthritis and diarrhea.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Genetics & Heredity
Anastasia-Lisa Dieckmann, Thomas Riedel, Boyke Bunk, Cathrin Sproeer, Joerg Overmann, Uwe Gross, Oliver Bader, Wolfgang Bohne, Burkhard Morgenstern, Morteza Hosseini, Andreas E. Zautner
Summary: The recent discovery of mixed Campylobacter coli strains suggests that C. coli and C. jejuni may be going through a process of accelerated gene transfer termed 'despeciation'. The phylogenetic tree revealed a separate cluster of Campylobacter isolates that possibly indicates genetic introgression. These hybrid strains, resulting from genetic introgression between C. coli and C. jejuni, have a genomic mosaic structure influenced by C. jejuni.
MICROBIAL GENOMICS
(2021)
Article
Virology
Sascha Dierks, Karin Thiele, Wolfgang Bohne, Raimond Lugert, Michael Weig, Uwe Gross, Nicolas von Ahsen, Julie Schanz, Andreas Fischer, Moritz Schnelle
Summary: This study reveals relevant analytical differences between SARS-CoV-2 qRT-PCR assays, leading to divergent decisions about the mandatory isolation of infected individuals. A strategy is proposed to harmonize qRT-PCR assays to achieve better comparability.
Article
Multidisciplinary Sciences
Matthias Frederik Emele, Sonja Smole Mozina, Raimond Lugert, Wolfgang Bohne, Wycliffe Omurwa Masanta, Thomas Riedel, Uwe Gross, Oliver Bader, Andreas Erich Zautner
SCIENTIFIC REPORTS
(2019)