Article
Multidisciplinary Sciences
Victoria V. Firstova, Anastasia S. Shakhova, Alena K. Riabko, Marina Silkina, Natalia A. Zeninskaya, Yana O. Romanenko, Maksim A. Marin, Methun M. Rogozin, Alena S. Kartseva, Ivan A. Dyatlov, Igor G. Shemyakin
Summary: The research studied the duration of antibodies specific to spores, protective antigen (PA), and lethal factor (LF) in the blood of donors after immunization with live anthrax vaccine. Antibodies against PA-D1 were found to circulate for over 1 year, while antibodies against LF and PA-D4 appeared after 11 months. Moderate positive correlation was observed between antibody titers and toxin neutralization activity in the blood serum samples.
Article
Microbiology
Kenneth Smith, Lori Garman, Kathleen Norris, Jennifer Muther, Angie Duke, Renata J. M. Engler, Michael R. Nelson, Limone C. Collins, Christina Spooner, Carla Guthridge, Judith A. James
Summary: Research has found that some individuals vaccinated with anthrax vaccine may produce antibodies with poor neutralizing capabilities, potentially due to increased IgG4 to IgG1 ratios, low antibody avidity, and insufficient antibody targeting specific regions. Future vaccine formulations and vaccination schedules should aim to improve these deficiencies.
Article
Chemistry, Multidisciplinary
Alisan Kayabolen, Ugur Akcan, Dogancan Ozturan, Hivda Ulbegi-Polat, Gizem Nur Sahin, Nareg Pinarbasi-Degirmenci, Canan Bayraktar, Gizem Soyler, Ehsan Sarayloo, Elif Nurtop, Berna Ozer, Gulen Guney-Esken, Tayfun Barlas, Ismail Selim Yildirim, Ozlem Dogan, Sercin Karahuseyinoglu, Nathan A. Lack, Mehmet Kaya, Cem Albayrak, Fusun Can, Ihsan Solaroglu, Tugba Bagci-Onder
Summary: Multimeric sACE2 demonstrates high neutralization efficiency and is a promising treatment approach against SARS-CoV-2 infections, surpassing the potency of monomeric sACE2 and effectively neutralizing variants of concern.
Article
Infectious Diseases
Norman L. Mukarati, Okechukwu C. Ndumnego, Sunday O. Ochai, Solomon Jauro, Andrew Loveridge, Henriette van Heerden, Gift Matope, Alexandre Caron, Tapiwa G. Hanyire, Michel de Garine-Wichatitsky, Davies M. Pfukenyi
Summary: This study investigated the presence of anthrax antibodies in African lions in Zimbabwe, finding that approximately 21.9% of lions tested positive for anthrax antibodies. The results suggest that anthrax may be more widespread in Zimbabwe than previously realized, present in both high- and low-risk zones, including areas where outbreaks have not been reported for over 20 years. The study highlights the need for improved surveillance of anthrax in both wild and domestic animals in order to better understand and control the disease.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Pharmacology & Pharmacy
Somayeh Ghotloo, Forough Golsaz-Shirazi, Mohammad Mehdi Amiri, Mahmood Jeddi-Tehrani, Fazel Shokri
Summary: The study investigated a human-mouse chimeric MAb c-1F2C2 for potential passive immunotherapy of tetanus, demonstrating its ability to neutralize tetanus toxin and partially prevent toxin-receptor binding. The chimeric MAb showed structural and functional similarities to its murine counterpart, suggesting its potential usefulness in tetanus treatment.
Article
Biochemical Research Methods
F. Hoelzgen, R. Zalk, R. Alcalay, S. Cohen-Schwartz, G. Garau, A. Shahar, O. Mazor, G. A. Frank
Summary: Anthrax infection is associated with severe illness and high mortality, with PA being a central component of the toxin. PA plays a crucial role in anthrax progression and is an important target for monoclonal antibody-based therapy. The cAb29 antibody neutralizes the toxin by preventing pore formation through interaction with the membrane-penetrating loop of PA.
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2021)
Article
Immunology
Gyu-Lee Kim, Seong Wook Pyo, Hwajung Yi, So-Hyeon Kim, Hwachul Shin, Min-Ah Yu, Yi-Rang Hwang, Sang-Yoon Choi, Jun Ho Jeon, Su Kyoung Jo, Gi-eun Rhie
Summary: A recombinant protective antigen anthrax vaccine (GC1109) is being developed as a new-generation vaccine. The booster dose of GC1109 was found to significantly increase the production of anti-protective antigen IgG and toxin-neutralizing antibody in mice. However, the booster dose did not enhance the protective effect, as the TNA titers of the non-booster group were already high enough to provide protection against spore challenge. The study also determined the threshold TNA titer levels associated with protection.
Article
Biophysics
Minghan Li, Shanchuan Chen, Yunjiao Wang, Shaoxia Zhang, Dandan Song, Rong Tian, Jia Geng, Liang Wang
Summary: In this study, a nanopore platform was fabricated to monitor intercellular protein transport and detect the activity of anthrax lethal factor (aLF) at a single molecule level. The aLF activity was successfully inhibited using screening approaches of protein-metal ion acquisition and other condition controlment. Ca2+, Mg2+, Mn2+, Ni2+ in collaboration with Zn2+ were found to promote aLF activity, while Cd2+, Co2+, Cu2+ showed inhibitory effects. The results also revealed that the speed of aLF endopeptidase activity with different ions functions as the nanopore signal frequency in a linear manner. In addition, the strength of the proton or hydroxide, as well as adapted temperature and gamma-ray, played integral roles in inhibiting aLF activity.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Multidisciplinary Sciences
Adam S. Olia, Cheng Cheng, Tongqing Zhou, Andrea Biju, Darcy R. Harris, Anita Changela, Hongying Duan, Vera B. Ivleva, Wing-Pui Kong, Li Ou, Reda Rawi, Yaroslav Tsybovsky, David J. Van Wazer, Angela R. Corrigan, Christopher A. Gonelli, Myungjin Lee, Krisha Mckee, Sandeep Narpala, Sijy O'Dell, Danealle K. Parchment, Erik-Stephane D. Stancofski, Tyler Stephens, Ivy Tan, I-Ting Teng, Shuishu Wang, Qing Wei, Yongping Yang, Zhengrong Yang, Baoshan Zhang, Jan Novak, Matthew B. Renfrow, Nicole A. Doria-Rose, Richard A. Koup, Adrian B. Mcdermott, Jason G. Gall, Q. Paula Lei, John R. Mascola, Peter D. Kwong
Summary: A study has found that introducing N-linked glycosylation sites can reduce the recognition of soluble HIV-1 envelope trimers. It was also observed that the introduced glycans make the trimers prone to disassembly and disorder. Additionally, only a small fraction of the antibody response to glycan-base trimers recognized protein-base trimers, and vice versa.
Article
Immunology
Alireza Felegary, Shahram Nazarian, Emad Kordbacheh, Javad Fathi, Mohamad Ebrahim Minae
Summary: This study evaluated the antibody response and protection of a recombinant chimeric protein against Shigella, finding that the protein produced in E. coli could be a promising candidate for vaccine development against Shigella. The chimeric protein showed high antibody response and neutralization ability against the bacterial toxin, providing significant protection in immunized mice against both S. flexneri and S.dysentery.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Immunology
Cecilia Abreu, Claudia Ortega, Natalia Olivero-Deibe, Federico Carrion, Aracelly Gaete-Argel, Fernando Valiente-Echeverria, Ricardo Soto-Rifo, Rafaela Milan Bonotto, Alessandro Marcello, Sergio Pantano
Summary: To combat emerging SARS-CoV-2 variants, a customizable pentameric scaffold was created based on a mammalian protein, capable of neutralizing Spike proteins from multiple viral particles simultaneously. With just two modules targeting the Spike's RBD domain, it outperforms human antibodies from vaccinated individuals and blocks virus entry. This multibody can be easily produced and contributes to therapeutic development and preparedness against evolving pathogens.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Virology
Jianhua Li, Xiaoyan Li, Haiyan Mao, Chen Huang, Yi Sun, Liangbin Miao, Jiaxuan Li, Wanchen Song, Yanjun Zhang, Jinsong Huang, Keda Chen
Summary: The emergence of the SARS-CoV-2 Omicron variant has raised concerns due to its numerous spike protein mutations that may reduce immune protection. The study found that the Omicron variant showed significantly higher immune evasion compared to the Delta variant, emphasizing the need for vaccines targeting Omicron.
Article
Immunology
Hari P. Dulal, David J. Vance, Durga P. Neupane, Xiangcheng Chen, Jacqueline M. Tremblay, Charles B. Shoemaker, Nicholas J. Mantis, Jeongmin Song
Summary: Researchers generated a VHH antibody library from typhoid toxoid-immunized alpacas and identified six potent antibodies that can neutralize typhoid toxin and protect mice from its toxicity.
INFECTION AND IMMUNITY
(2022)
Article
Chemistry, Multidisciplinary
Zeyu Lu, Nicholas L. Truex, Mariane B. Melo, Yiran Cheng, Na Li, Darrell J. Irvine, Bradley L. Pentelute
Summary: Novel immunotoxins were developed by linking full-length IgG antibodies with nontoxic anthrax proteins, showing efficient and potent activity against malignant cells with high specificity for HER2 and EGFR, as well as increased pharmacokinetics and in vivo safety, suggesting promise for further therapeutic development.
ACS CENTRAL SCIENCE
(2021)
Article
Pharmacology & Pharmacy
Tania Maria Manieri, Daniela Yumi Takata, Roselaine Campos Targino, Wagner Quintilio, Joao Victor Batalha-Carvalho, Camila Maria Lucia da Silva, Ana Maria Moro
Summary: This study identified a panel of neutralizing monoclonal antibodies (mAbs) against tetanus toxin (TeNT), some of which can neutralize the toxin and prevent symptoms and death in mice. Two mAbs were found to impair the binding of TeNT to the GT1b ganglioside receptor. Stable cell lines were generated to produce large quantities of mAbs, and their characteristics were analyzed.
Article
Gastroenterology & Hepatology
Zhipeng Yan, Jing Zeng, Youjun Yu, Kunlun Xiang, Hui Hu, Xue Zhou, Lili Gu, Li Wang, Jie Zhao, John A. T. Young, Lu Gao
JOURNAL OF HEPATOLOGY
(2017)
Article
Chemistry, Medicinal
Zongxing Qiu, Xianfeng Lin, Weixing Zhang, Mingwei Zhou, Lei Guo, Buelent Kocer, Guolong Wu, Zhisen Zhang, Haixia Liu, Houguang Shi, Buyu Kou, Taishan Hu, Yimin Hu, Mengwei Huang, S. Frank Yan, Zhiheng Xu, Zheng Zhou, Ning Qin, Yue Fen Wang, Shuang Ren, Hongxia Qiu, Yuxia Zhang, Yi Zhang, Xiaoyue Wu, Kai Sun, Sheng Zhong, Jianxun Xie, Giorgio Ottaviani, Yuan Zhou, Lina Zhu, Xiaojun Tian, Liping Shi, Fang Shen, Yi Mao, Xue Zhou, Lu Gao, John A. T. Young, Jim Zhen Wu, Guang Yang, Alexander V. Mayweg, Hong C. Shen, Guozhi Tang, Wei Zhu
JOURNAL OF MEDICINAL CHEMISTRY
(2017)
Article
Medicine, Research & Experimental
Hassan Javanbakht, Henrik Mueller, Johanna Walther, Xue Zhou, Anais Lopez, Thushara Pattupara, Julie Blaising, Lykke Pedersen, Nanna Albaek, Malene Jackerott, Tianlai Shi, Corinne Ploix, Wouter Driessen, Robert Persson, Jacob Ravn, John A. T. Young, Soren Ottosen
MOLECULAR THERAPY-NUCLEIC ACIDS
(2018)
Review
Chemistry, Medicinal
Song Feng, Lu Gao, Xingchun Han, Taishan Hu, Yimin Hu, Haixia Liu, Andrew W. Thomas, Zhipeng Yan, Song Yang, John A. T. Young, Hongying Yun, Wei Zhu, Hong C. Shen
ACS INFECTIOUS DISEASES
(2018)
Article
Gastroenterology & Hepatology
Zhipeng Yan, Daitze Wu, Hui Hu, Jing Zeng, Xin Yu, Zhiheng Xu, Zheng Zhou, Xue Zhou, Guang Yang, John A. T. Young, Lu Gao
Article
Gastroenterology & Hepatology
Henrik Mueller, Anais Lopez, Philipp Tropberger, Steffen Wildum, Josephine Schmaler, Lykke Pedersen, Xingchun Han, Yongguang Wang, Soren Ottosen, Song Yang, John A. T. Young, Hassan Javanbakht
Article
Multidisciplinary Sciences
Alan J. Mueller-Breckenridge, Fernando Garcia-Alcalde, Steffen Wildum, Saskia L. Smits, Robert A. de Man, Margo J. H. van Campenhout, Willem P. Brouwer, Jianjun Niu, John A. T. Young, Isabel Najera, Lina Zhu, Daitze Wu, Tomas Racek, Gadissa Bedada Hundie, Yong Lin, Charles A. Boucher, David van de Vijver, Bart L. Haagmans
SCIENTIFIC REPORTS
(2019)
Article
Gastroenterology & Hepatology
Jing Zeng, Daitze Wu, Hui Hu, John A. T. Young, Zhipeng Yan, Lu Gao
Review
Microbiology
Jay A. Grobler, Annaliesa S. Anderson, Prabhavathi Fernandes, Michael S. Diamond, Christine M. Colvis, Joseph P. Menetski, Rosa M. Alvarez, John A. T. Young, Kara L. Carter
CELL HOST & MICROBE
(2020)
Article
Cell Biology
Simon Langer, Xin Yin, Arturo Diaz, Alex J. Portillo, David E. Gordon, Umu H. Rogers, John M. Marlett, Nevan J. Krogan, John A. T. Young, Lars Pache, Sumit K. Chanda
Review
Pharmacology & Pharmacy
Timothy M. Block, John A. T. Young, Hassan Javanbakht, Michael J. Sofia, Tianlun Zhou
Summary: This paragraph introduces a new method of using dihydroquinolizinone to inhibit PAPD5 and PAPD7 polymerases for HBV RNA degradation, potentially developing a new class of HBV antivirals. PAPD5 and PAPD7 play crucial roles in RNA quality control pathway, which could be targeted as an antiviral strategy.
ANTIVIRAL RESEARCH
(2021)
Article
Cell Biology
Jeffrey R. Johnson, David C. Crosby, Judd F. Hultquist, Andrew P. Kurland, Prithy Adhikary, Donna Li, John Marlett, Justine Swann, Ruth Huttenhain, Erik Verschueren, Tasha L. Johnson, Billy W. Newton, Michael Shales, Viviana A. Simon, Pedro Beltrao, Alan D. Frankel, Alexander Marson, Jeffery S. Cox, Oliver Fregoso, John A. T. Young, Nevan J. Krogan
Summary: Viruses remodel host cellular pathways to replicate and evade immune defenses and they do so with limited genomic coding capacity. This study used mass spectrometry-based proteomics to analyze protein abundance and post-translational modifications in response to HIV-1 infection. The results revealed the importance of Aurora kinase activity in HIV-1 infection and identified potential substrates of a degraded phosphatase during infection. Additionally, the study showed that the HIV-1 Vpr protein inhibits histone H1 ubiquitination, leading to DNA repair defects.
Article
Immunology
Steffen Wildum, Kyle E. Korolowicz, Manasa Suresh, Guido Steiner, Lue Dai, Bin Li, Changsuek Yon, Maria Cristina De Vera Mudry, Franziska Regenass-Lechner, Xu Huang, Xupeng Hong, Marta G. Murreddu, Bhaskar V. Kallakury, John A. T. Young, Stephan Menne
Summary: RG7854 shows potential for treating chronic hepatitis B in woodchucks, suppressing viral replication, inducing immune responses, and producing sustained antiviral effects. Combination treatment with entecavir does not enhance the therapeutic efficacy of RG7854, but augments the magnitude of immune responses.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Gastroenterology & Hepatology
Stephan Menne, Steffen Wildum, Guido Steiner, Manasa Suresh, Kyle Korolowicz, Maria Balarezo, Changsuek Yon, Marta Murreddu, Xupeng Hong, Bhaskar Kallakury, Robin Tucker, Song Yang, John A. T. Young, Hassan Javanbakht
HEPATOLOGY COMMUNICATIONS
(2020)
Article
Gastroenterology & Hepatology
Henrik Mueller, Steffen Wildum, Souphalone Luangsay, Johanna Walther, Anais Lopez, Philipp Tropberger, Giorgio Ottaviani, Wenzhe Lu, Neil John Parrott, Jitao David Zhang, Roland Schmucki, Tomas Racek, Jean-Christophe Hoflack, Erich Kueng, Floriane Point, Xue Zhou, Guido Steiner, Marc Luetgehetmann, Gianna Rapp, Tassilo Volz, Maura Dandri, Song Yang, John A. T. Young, Hassan Javanbakht
JOURNAL OF HEPATOLOGY
(2018)
