Article
Immunology
Elisabetta Mantuano, Pardis Azmoon, Michael A. Banki, Christina J. Sigurdson, Wendy M. Campana, Steven L. Gonias
Summary: This study demonstrates that a soluble derivative of PrPC (S-PrP) can counteract inflammatory responses triggered by pattern recognition receptors in macrophages, and significantly attenuate the toxicity of LPS in mice. The response of macrophages to S-PrP is mediated by a receptor assembly that includes the N-methyl-D-aspartate receptor (NMDA-R) and low-density lipoprotein receptor-related protein-1 (LRP1). PrPC was identified in extracellular vesicles (EVs) isolated from human plasma, which replicated the activity of S-PrP in inhibiting cytokine expression and I kappa B alpha phosphorylation in LPS-treated macrophages. The effects of plasma EVs on LPS-treated macrophages were blocked by PrPC-specific antibodies and inhibitors of LRP1 and the NMDA-R, and were also abolished by deleting Lrp1 in macrophages and inhibiting Src family kinases.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Simona Delle Monache, Fanny Pulcini, Francesca Santilli, Stefano Martellucci, Costantino Santacroce, Jessica Fabrizi, Adriano Angelucci, Maurizio Sorice, Vincenzo Mattei
Summary: Studies suggest that hypoxia treatment of DPSCs promotes their differentiation into neuronal cells in a time-dependent manner. Additionally, the conditioned media from hypoxia-stimulated DPSCs can induce neural differentiation in other neural cells.
Article
Cell Biology
Daniela Peruzzu, Zaira Boussadia, Federica Fratini, Francesca Spadaro, Lucia Bertuccini, Massimo Sanchez, Maria Carollo, Paola Matarrese, Mario Falchi, Francesca Iosi, Carla Raggi, Isabella Parolini, Alessandra Care, Massimo Sargiacomo, Maria Cristina Gagliardi, Katia Fecchi
Summary: In this study, the role of cholesterol transport in the endosomal degradative-secretory system was explored using the U18666A inhibitor in a metastatic human melanoma cell line (WM266-4). The results showed that U18666A induced a shift of Cav-1 from the plasma membrane to the endolysosomal compartment, affecting the formation and release of small extracellular vesicles (sEVs). The inhibitor also altered the protein composition of sEVs and reduced their transfer capacity on target cells.
Review
Medicine, General & Internal
Antonella Capozzi, Valeria Manganelli, Gloria Riitano, Daniela Caissutti, Agostina Longo, Tina Garofalo, Maurizio Sorice, Roberta Misasi
Summary: The pathological features of APS are caused by the activity of aPLs associated with vascular thrombosis and obstetric complications. These aPLs not only serve as disease markers, but also have a determining pathogenetic role in APS by activating cells and coagulation factors. This activation occurs through the interaction of aPLs with specific target receptors on the cell membrane known as lipid rafts.
JOURNAL OF CLINICAL MEDICINE
(2023)
Editorial Material
Biochemistry & Molecular Biology
Vincenzo Mattei, Simona Delle Monache
Review
Biochemistry & Molecular Biology
Francesca Santilli, Jessica Fabrizi, Fanny Pulcini, Costantino Santacroce, Maurizio Sorice, Simona Delle Monache, Vincenzo Mattei
Summary: This review critically analyzes the role of gangliosides in the differentiation of mesenchymal stem cells and examines their potential use as specific cell surface markers.
Article
Oncology
Alessandra Fragale, Emilia Stellacci, Giulia Romagnoli, Valerio Licursi, Stefania Parlato, Irene Canini, Giacomo Remedi, Maria Buoncervello, Paola Matarrese, Lucia Pedace, Barbara Ascione, Simone Pizzi, Marco Tartaglia, Stefania D'Atri, Carlo Presutti, Imerio Capone, Lucia Gabriele
Summary: BRAF(V600) mutations are commonly found in melanoma cells and support various oncogenic processes. The combination of romidepsin and IFN-a2b reduces melanoma proliferation, survival, and invasion, and overcomes resistance to vemurafenib. This combination treatment modulates genetic and immune pathways, restores immune signals, induces cell death, and enhances the immunogenic potential of resistant melanoma cells, providing a potential strategy for treating BRAFi-resistant metastatic melanoma.
INTERNATIONAL JOURNAL OF CANCER
(2023)
Article
Cell Biology
Paola Matarrese, Rosa Vona, Barbara Ascione, Camilla Cittadini, Annalisa Tocci, Anna Maria Mileo
Summary: Dynamic reciprocity between cellular components of the tumor microenvironment and tumor cells occurs through the interaction of soluble signals. This study focuses on the functional response of non-small cell lung cancer cell lines treated with stromal factors that mimic the stimulus exerted in vivo on tumor cells. The results highlight the role of the autophagic machinery and the YAP pathway in the mutual crosstalk between tumor cells and the tumor microenvironment.
Review
Cell Biology
Niccolo Candelise, Francesca Santilli, Jessica Fabrizi, Daniela Caissutti, Zaira Spinello, Camilla Moliterni, Loreto Lancia, Simona Delle Monache, Vincenzo Mattei, Roberta Misasi
Summary: Despite the lack of available therapies for neurodegenerative disorders, cell therapy using stem cells from various sources, such as dental pulp stem cells, has shown promise in preclinical models. These stem cells can be reprogrammed for neural differentiation and have potential applications in the treatment of nervous system disorders. This review summarizes the advancements and differences in the maintenance, differentiation, and potential clinical application of such stem cells.
Article
Cell Biology
Valeria Manganelli, Roberta Misasi, Gloria Riitano, Antonella Capozzi, Vincenzo Mattei, Tuba Rana Caglar, Davide Ialongo, Valentina Noemi Madia, Antonella Messore, Roberta Costi, Roberto Di Santo, Maurizio Sorice, Tina Garofalo
Summary: This research investigated a new HPSE inhibitor, RDS 3337, and its role in regulating the autophagic process and the balance between apoptosis and autophagy in U87 glioblastoma cells. The results showed that RDS 3337 inhibited autophagic-lysosomal flux, leading to the accumulation of lipidated LC3-II form. This inhibition of autophagic flux activated apoptosis mechanisms. These findings suggest a potential role for HPSE inhibitors in controlling tumor growth progression by balancing apoptosis and autophagy.
Editorial Material
Biochemistry & Molecular Biology
Vincenzo Mattei, Simona Delle Monache
Article
Cell Biology
Claudia Abbruzzese, Silvia Matteoni, Paola Matarrese, Michele Signore, Barbara Ascione, Elisabetta Iessi, Aymone Gurtner, Andrea Sacconi, Lucia Ricci-Vitiani, Roberto Pallini, Andrea Pace, Veronica Villani, Andrea Polo, Susan Costantini, Alfredo Budillon, Gennaro Ciliberto, Marco G. Paggi
Summary: This study reveals the mechanism of action of chlorpromazine (CPZ) in the treatment of glioblastoma (GBM), with PKM2 identified as its major target. CPZ can alter energy metabolism in GBM cells and inhibit their oncogenic properties, while having minimal effects on non-cancerous neuroepithelial cells.
CELL DEATH & DISEASE
(2023)
Meeting Abstract
Clinical Neurology
Stefano Martellucci, Mark Carter, Andi Flutsch, Elisabetta Mantuano, Steven Gonias, Wendy Campana
JOURNAL OF THE PERIPHERAL NERVOUS SYSTEM
(2022)