Article
Microbiology
Hye Seon Song, Juseok Kim, Yeon Bee Kim, Se Hee Lee, Tae Woong Whon, Seong Woon Roh
Summary: Two novel halophilic archaeal strains were isolated from solar salt in South Korea. Through genetic, physiological, and biochemical analyses, they were found to represent a new genus and species in the family Halobacteriaceae.
JOURNAL OF MICROBIOLOGY
(2022)
Article
Microbiology
Muhtar Dilmurat, Xin-Yu Hu, Tohty Dilbar, Heng-Lin Cui
Summary: A halophilic archaeal strain, designated C46(T), was isolated from an inland salt lake in Qinghai Province, PR China. The phylogenetic analysis based on 16S rRNA gene sequences showed that strain C46(T) belongs to the genus Halobaculum and is closely related to Halobaculum rubrum strain WSA2(T).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2022)
Article
Microbiology
Bing-Bing Liu, Nimaichand Salam, Manik Prabhu Narsing Rao, Shuang Cheng, Yuan-Guo Xie, Lai-You Wang, Yuan-ru Zhang, Xin-Yuan Yu, Shu-xian Guo, Wen-Jun Li
Summary: Two novel halophilic strains were isolated from a saline sediment sample in Aiding salt-lake, China. These strains, named Haloterrigena gelatinilytica, showed high salt and temperature tolerance.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Microbiology
Zhenqiang Zuo, Dahe Zhao, Jian Zhou, Jing Han, Hua Xiang
Summary: Strain N1521(T) is a novel extremely halophilic archaeon with resistance to salt stress, exhibiting a wide range of adaptability in terms of temperature, pH, and salinity, and capable of producing red pigments.
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
(2021)
Article
Environmental Sciences
Ya-Ping Sun, Bei-Bei Wang, Zhang-Ping Wu, Xi-Wen Zheng, Jing Hou, Heng-Lin Cui
Summary: Five novel halophilic archaeal strains were isolated from coastal saline sediments and sea salt in China. They demonstrated typical morphology and growth characteristics and showed high similarities with existing species. However, based on genetic and phylogenetic analyses, it is confirmed that these strains represent five new species. This is the first report of a novel haloarchaeon isolated from a marine intertidal zone.
FRONTIERS IN MARINE SCIENCE
(2023)
Article
Microbiology
Zhang-Ping Wu, Ya-Ping Sun, Bei-Bei Wang, Jing Hou, Heng-Lin Cui
Summary: A filamentous cell-shaped halophilic archaeon was isolated from Aiding Salt Lake, PR China. It was found to be closely related to Halocatena pleomorpha based on phenotypic, phylogenetic, and genomic analyses. This newly discovered species was named Halocatena salina.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2022)
Article
Microbiology
Chen-Xi Bao, Si-Ya Li, Yu-Jie Xin, Jing Hou, Heng-Lin Cui
Summary: Four halophilic archaeal strains, YPL8(T), SLN56(T), LT61(T), and KZCA68(T), were isolated from different regions in China. Three of these strains belong to a new species of the genus Natrinema, named Natrinema halophilum sp. nov., Natrinema salinisoli sp. nov., and Natrinema amylolyticum sp. nov. The fourth strain is a new species of the genus Haloterrigena, named Haloterrigena alkaliphila sp. nov.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2022)
Article
Microbiology
Ho Le Han, Reneelyn E. Danganan, Zhun Li, Na-Ri Shin, Reuel M. Bennett, Gina R. Dedeles, Song-Gun Kim
Summary: A novel extremely halophilic archaeon, strain RHB-C-T, was isolated from a saturated brine pond in Bolinao, Pangasinan, Philippines. It belongs to the genus Halorubrum and is proposed as a new species Halorubrum salinarum sp. nov.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2022)
Article
Microbiology
Bei-Bei Wang, Chen- Xi Bao, Ya-Ping Sun, Jing Hou, Heng-Lin Cui
Summary: Two novel halophilic archaeal strains, Gai3-17T and XZYJT26T, were isolated from the sediments of Gaize salt lake and the saline soil of Mangkang ancient solar saltern in Tibet, PR China, respectively. These strains represent two novel species of Halobacterium, which have been named as Halobacterium wangiae sp. nov. and Halobacterium zhouii sp. nov.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Xin-Xin Li, Shun Tan, Mu Cheng, Yao Hu, Xue Ma, Jing Hou, Heng-Lin Cui
Summary: Four halophilic archaea strains were isolated from salt and soda lakes in China. These strains were genetically distinct from existing genera and may represent new species and genera within the family Natrialbaceae. Phenotypic characteristics and lipid profiles also differentiated these strains from related genera.
Article
Biochemistry & Molecular Biology
Bing-Bing Liu, Nimaichand Salam, Shuang Cheng, Wen-qing Zhang, Yong-qing Zhou, Shu-xian Guo, Wen-Jun Li
Summary: Two extremely halophilic archaea isolates, SYSU A00711(T) and SYSU A00630, were obtained from sediment soil sample collected from Aiding lake in China. They are closely related to Halegenticoccus soli based on phenotypic, chemotaxonomic, and phylogenetic properties. The isolates are proposed to represent a novel species in the genus Halegenticoccus, named as Halegenticoccus tardaugens sp. nov.
Article
Biotechnology & Applied Microbiology
Jing Hou, Si-Ya Li, Yang-Jie Zhao, Heng-Lin Cui
Summary: This study identified a novel type of halolysin (Hly(Hap)) without C-terminal extension (CTE) from the extremely halophilic archaeon Haladaptatus sp. DYF46. Hly(Hap) exhibited stability and optimal activity in a low salinity condition despite being derived from an extremely halophilic archaeon. It was also found that Ca2+ and Mg2+ significantly promoted the activity of Hly(Hap). Moreover, Hly(Hap) showed stability in the presence of organic solvents and detergents.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jing Hou, Xue-Meng Yin, Yang Li, Dong Han, Bu Lu, Jia-Yi Zhang, Heng-Lin Cui
Summary: The extracellular protease HlyA from Halococcus salifodinae exhibited good temperature, pH, and salinity tolerance, with significant enhancements in activity by Ca2+, Mg2+, and Sr2+. It was completely inhibited by phenylmethanesulfonyl fluoride (PMSF), indicating it is a serine protease. Additionally, HlyA showed good tolerance to some surfactants and organic solvents, making it a promising candidate for industrial applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Microbiology
D. Barrie Johnson, David S. Holmes, Eva Vergara, Roseanne Holanda, Eva Pakostova
Summary: Ten acidophilic bacteria strains, which form a monophyletic clade within Firmicutes, were isolated from different environments. Comparison of their complete genomes revealed two proposed genera (Sulfoacidibacillus and Ferroacidibacillus) and three proposed species (Sulfoacidibacillus ferrooxidans, Sulfoacidibacillus thermotolerans, and Ferroacidibacillus organovorans). These bacteria strains share physiological traits such as iron oxidation and reduction, as well as being obligately heterotrophic. Additionally, they exhibit different degrees of acid tolerance and temperature preferences. The importance of these findings is rated as 8 out of 10.
RESEARCH IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Bei-Bei Wang, Ya-Ping Sun, Zhang-Ping Wu, Xi-Wen Zheng, Jing Hou, Heng-Lin Cui
Summary: Three new species of the Halorientalis genus, Halorientalis salina sp. nov., Halorientalis marina sp. nov., and Halorientalis litorea sp. nov., were identified from three halophilic archaeal strains isolated from a salt lake in Tibet and coarse sea salt samples from Guangdong and Hebei, China. These strains showed distinct genetic and phenotypic characteristics, differentiating them from current Halorientalis species.
Article
Multidisciplinary Sciences
Zahra Goodarzi, Sedigheh Asad, Maliheh Mehrshad
Summary: Aquatic microbial communities serve as an important reservoir for antibiotic resistance genes (ARGs), but the distribution and diversity of different ARG categories in environmental microbes with different ecological strategies are not well studied. In this study, the Caspian Sea metagenomes were analyzed using a combination of computational methods to comprehensively screen the diversity and distribution of ARGs. The results revealed the dominant ARG categories and the characteristics of representative taxa. It was found that certain bacterial taxa modify the antibiotic target via mutation to develop antibiotic resistance. This study provides insights into the alignment between encoded resistance categories and ecological strategies of microbial genomes.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Roohollah Kheiri, Maliheh Mehrshad, Ahmad Ali Pourbabaee, Antonio Ventosa, Mohammad Ali Amoozegar
Summary: Lake Urmia in Iran is a hypersaline environment with a salinity of about 27%. Metagenomic analysis revealed that the microbial community in the lake is dominated by Haloferacaceae family (69.8%), mainly Haloquadratum (59.3%) and Halonotius (9.1%). The bacterial community is mainly composed of Salinibacter ruber (23.3%). Genomic variation analysis showed a high level of SNVs and insertions in Lake Urmia's community, possibly due to transformation caused by the extreme conditions and high ionic concentrations.
SCIENTIFIC REPORTS
(2023)
Article
Microbiology
Vinicius S. S. Kavagutti, Paul-Adrian Bulzu, Cecilia M. M. Chiriac, Michaela M. M. Salcher, Indranil Mukherjee, Tanja Shabarova, Vesna Grujcic, Maliheh Mehrshad, Vojtech Kasalicky, Adrian-Stefan Andrei, Jitka Jezberova, Jaromir Seda, Pavel Rychtecky, Petr Znachor, Karel Simek, Rohit Ghai
Summary: This study performed high-resolution sampling of the spring bloom in a freshwater reservoir and identified previously unknown microbial taxa using metagenome-assembled genomes. The recovered genomes provide insights into the distributional dynamics and doubling times of microbial lineages during the bloom. Additionally, the study uncovered the presence of aplastidic cryptophytes and giant viruses, offering new understanding of plankton dynamics.
Article
Plant Sciences
Somayeh Rasoli-dogaheh, Mostafa Noroozi, Javad Khansha, Mohammad Ali Amoozegar, Rezvan Rahimi, Mahdi Moshtaghi Nikou, Tomas Hauer
Summary: A study on the diversity of cyanobacteria in the rainwater basin of the Persian Gulf region found a novel taxon, Khargia iranica, on Kharg Island. Similar strains were also found in South Italy and India, leading to the recognition of the new genus Khargia. Different analytical methods were used to identify and classify the new species.
Correction
Multidisciplinary Sciences
Roohollah Kheiri, Maliheh Mehrshad, Ahmad Ali Pourbabaee, Antonio Ventosa, Mohammad Ali Amoozegar
SCIENTIFIC REPORTS
(2023)
Article
Microbiology
Maryam Yavari-Bafghi, Maryam Rezaei Somee, Mohammad Ali Amoozegar, Seyed Mohammad Mehdi Dastgheib, Mahmoud Shavandi
Summary: This study investigated the removal of phenol using Small Bioreactor Chambers and CaO2 nanoparticles in continuous-flow sand-packed columns. The results showed that simultaneous biostimulation and bioaugmentation could completely eliminate phenol within 42 days, while natural bioremediation columns still had 80.2% remaining at the end of the experiment. CaO2 injection decreased microbial diversity, with order-level groups such as Rhodobacterales and Xanthomonadales dominating in biostimulation columns. Long-term exposure to phenol shifted the microbial population towards heterotrophic members with the potential for phenol and hydrocarbon degradation.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Krzysztof T. Jurdzinski, Maliheh Mehrshad, Luis Fernando Delgado, Ziling Deng, Stefan Bertilsson, Anders F. Andersson
Summary: The crossing of environmental barriers poses adaptive challenges and bacterial communities in freshwater-marine transitions are rarely related to brackish counterparts. This study conducted a phylogenomic analysis of metagenome-assembled genomes to investigate the molecular adaptations facilitating cross-biome transitions. The results showed that cross-biome transitions were rare, ancient, and mostly directed toward the brackish biome, accompanied by systematic changes in proteomes and gene functions.
Review
Microbiology
Elham Lashani, Mohammad Ali Amoozegar, Raymond J. Turner, Hamid Moghimi
Summary: Microorganisms play a crucial role in reducing metalloid contamination risks through their ability to tolerate and detoxify these elements.
Article
Microbiology
Alicia Garcia-Roldan, Rafael R. de la Haba, Blanca Vera-Gargallo, Cristina Sanchez-Porro, Antonio Ventosa
Summary: Metagenomic sequences of prokaryotic microbiota from brine of a crystallizer pond in a saltern in Isla Cristina, southwest Spain, were obtained using Illumina. Haloarchaea and Salinibacter were the most abundant prokaryotes.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Article
Environmental Sciences
Elham Lashani, Hamid Moghimi, Raymond J. Turner, Mohammad Ali Amoozegar
Summary: Selenium oxyanions are released into environments from various activities and are present in wastewater in many locations worldwide. Excessive amounts of selenium have negative impacts on living organisms. Halophilic and halotolerant microorganisms were used for selenium oxyanion remediation in wastewater containing salt. Factors such as aeration, carbon sources, competitive electron acceptors, and reductase inhibitors were investigated for their effects on SeO32- removal. The study also explored the remediation of SeO32- in agricultural effluents containing NO3-. The results showed that aerobic conditions with succinate as a carbon source were most effective for SeO32- removal. Certain ions, such as WO42- and TeO32-, decreased the removal percentage, while SO42- and PO43- had minimal interference. NO3- had an adverse effect on SeO32- biotransformation. Consortia of halophilic/halotolerant bacteria and yeasts were able to reduce SeO32- in synthetic agricultural wastewater by 45-53% within 120 hours. This study suggests that these consortia could be used for the bioremediation of selenium-contaminated drainage water.
ENVIRONMENTAL POLLUTION
(2023)
Article
Multidisciplinary Sciences
Atefeh Safarpour, Marzieh Ebrahimi, Seyed Abolhassan Shahzadeh Fazeli, Mohammad Ali Amoozegar
Summary: In recent years, there has been growing interest in the anticancer properties of metabolites from halophilic microorganisms. This study investigated the effects of supernatant metabolites from 29 halophilic bacterial strains on the stem cell-like properties of six human cancer cell lines. The metabolites from Salinivenus iranica demonstrated the most potent cytotoxic effect on breast cancer cell lines, with no effect on normal cells. The purified cytotoxic molecule, a phenol amine, downregulated the expression of the pluripotency gene SOX-2 in breast cancer cells.
SCIENTIFIC REPORTS
(2023)
Article
Ecology
Carlotta Meriggi, Maliheh Mehrshad, Richard K. Johnson, Ane T. Laugen, Stina Drakare
Summary: Species distribution models calibrated with bioclimatic variables suggest a high probability of range expansion of the invasive toxin producing cyanobacterium Raphidiopsis raciborskii to Sweden. However, field-based surveys and in-silico screening did not fully support this prediction, indicating the need for proactive monitoring with high temporal and spatial frequency. The inconsistencies between the models and monitoring approaches highlight the uncertainties in solely focusing on climate drivers and the potential limitations of early invasion detection.
ISME COMMUNICATIONS
(2023)
Article
Biotechnology & Applied Microbiology
Yeganeh Farrokhi, Zeinab Neshati, Parastoo Saniee, Ali Makhdoumi
Summary: This study found that two recently isolated probiotic strains, Bacillus sp. 1630F and Enterococcus sp. 7C37, have the ability to form biofilm and combat H. pylori biofilm formation. Compared to reference probiotics, Lactobacillus casei and Bifidobacterium bifidum, Bacillus and Enterococcus strains showed higher biofilm rates. They were also more effective in inhibiting H. pylori biofilm formation on polystyrene and AGS cells. Bacillus and Enterococcus strains achieved inhibition by promoting the expression of luxS and decreasing the expression of ropD.
INTERNATIONAL MICROBIOLOGY
(2023)