Flectobacillus rhizosphaerae sp nov., isolated from the rhizosphere soil of Oryza sativa (L.), and emended description of the genus Flectobacillus

A Gram-stain-negative, aerobic, pale orange, rod-shaped, non-motile bacterial strain, designated JC289(T) was isolated from a rhizosphere soil sample of the plant Oryza sativa (L.). 16S rRNA gene sequence analysis clearly allocated strain JC289(T) to the Flectobacillus cluster, showing highest sequence similarities to Flectobacillus roseus GFA-11(T) (99.5%), Flectobacillus major ATCC 29496(T) (96.7 %) and Flectobacillus lacus CL-GP79(T) (94.6 %). Major (>5%) fatty acids of strain JC289(T) were C-16:1 omega 5c, iso-C-15:0, C-15:0 2-OH, iso-C-15:0 3-OH and C-16:1 omega 7C/C-16:1 omega 6c, with minor amounts (>1-<5%) of C-14:0, C-16:0, anteiso-C-15:0, C-15:0, C-16:0 3-OH, iso-C-17:0 3-OH, anteiso-C17:1B and/or iso-C17:1I. Menaquinone-7 was the major quinone of strain JC289(T), and the polar lipid profile contained phosphatidylethanolamine, five unidentified aminophospholipids, two unidentified phospholipids, two unidentified aminolipids, two unidentified glycolipids and five unidentified lipids. DNA DNA relatedness values of strain JC289(T) with Flectobacillus roseus LMG 24501(T) and Flectobacillus major LMG 13163(T) were 24.6% (31.4%, reciprocal analysis) and 16.2% (22.4%), respectively. On the basis of 16S rRNA gene sequence analysis, physiological and biochemical test results, and chemotaxonomic analysis, strain JC289(T) can be differentiated from its closest relatives in the genus Flectobacillus. Based on the data presented, it is concluded that strain JC289T represents a novel species of the genus Flectobacillus, for which the name Flectobacillus rhizosphaerae sp. nov. is proposed. The type strain is JC289(T) (=KCTC 42575(T)=LMG 28712(T)).