Article
Biochemistry & Molecular Biology
Andrea Weinzierl, Yves Harder, Daniel Schmauss, Michael D. Menger, Matthias W. Laschke
Summary: This study demonstrates the tissue-protective effects of bromelain in musculocutaneous flaps, including reducing flap necrosis, increasing functional microvessel density, and suppressing ischemia-induced inflammation and apoptosis.
Article
Chemistry, Analytical
Xiaoling Su, Deying Chen, Liang Li, Lanjuan Li
Summary: The study demonstrates that perfusion can significantly alter the liver metabolome, recommending the use of non-perfused liver for comprehensive liver metabolomics analysis for high coverage.
ANALYTICA CHIMICA ACTA
(2021)
Editorial Material
Cell Biology
Christopher Schrecker, Andrew M. Intlekofer
Summary: The study identified a selective requirement for pyruvate dehydrogenase (PDH) activity in T cells and T cell leukemia, but not in hematopoietic stem cells (HSCs) or myeloid leukemia, supporting the idea that cell lineage plays a key role in cancer cell metabolism.
Review
Biochemistry & Molecular Biology
Xiao-yu Wu, Yi-miao Zhu, Yang Qi, Wen-Wen Xu, Jing-Zhai
Summary: Tissue engineering aims to provide scaffolds mimicking native tissues, with erythropoietin (EPO) playing an increasingly significant role in regenerative medicine by preventing cell death and providing tissue protection. This review critically analyzes techniques for modifying scaffolds with EPO, as well as non-hematopoietic functions of EPO, discussing applications and future prospects in regenerative medicine.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Surgery
Andrea Weinzierl, Yves Harder, Michael D. Menger, Matthias W. Laschke
Summary: This study found that intermittent fasting can effectively prevent flap necrosis by increasing angiogenesis and reducing inflammatory responses.
PLASTIC AND RECONSTRUCTIVE SURGERY
(2023)
Article
Immunology
Xue Zhang, Dan He, Jialin Jia, Feihong Liang, Jie Mei, Wenhua Li, Tingting Liu, Zhiyu Wang, Yu Liu, Fengxue Zhang, Zhiren Zhang, Bangwei Luo
Summary: Initial exposure to LPS can induce a hypo-responsive state in macrophages, known as endotoxin tolerance. Recent findings show that endotoxin-tolerant macrophages undergo functional re-programming, leading to enhanced protective functions. This study investigates the potential effects of Erythropoietin (EPO) on the functional re-programming of endotoxin-tolerant macrophages. The results reveal that EPO can enhance tolerance in macrophages and mice, improve host protective genes, and alleviate lung injury in a septic model of mice. Overall, targeting EPO may be a new therapeutic option in sepsis and other inflammatory disorders.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Zhongnan Yang, Lei Yan, Huixia Cao, Yue Gu, Pan Zhou, Mingyue Shi, Guodong Li, Xiaojing Jiao, Na Li, Xiangnan Li, Kai Sun, Fengmin Shao
Summary: The study showed that erythropoietin (EPO) can alleviate diffuse alveolar hemorrhage (DAH) by reducing macrophage recruitment and promoting phenotype switch, suggesting its potential as a therapeutic agent for DAH. Further research is needed to validate the direct target of miR-494-3p in regulating JAK2/STAT3 signaling transduction.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Biology
Guangfeng Geng, Jinhua Liu, Changlu Xu, Yandong Pei, Linbo Chen, Chenglong Mu, Ding Wang, Jie Gao, Yue Li, Jing Liang, Tian Zhao, Chuanmei Zhang, Jiaxi Zhou, Quan Chen, Yushan Zhu, Lihong Shi
Summary: Erythropoietin (EPO) is crucial for erythropoiesis, and mitochondrial quality control, specifically through the mitophagy receptor FUNDC1, plays a significant role in EPO-driven erythropoiesis. Impaired FUNDC1 function leads to the accumulation of damaged mitochondria, elevated ROS levels, inflammatory responses, and decreased EPO production in renal anemia. This study suggests that targeting mitochondrial quality control mechanisms could be a potential therapeutic strategy for treating renal anemia.
Article
Surgery
Han Gyu Cha, Joon Hur, Chris Ahn, Joon Pio Hong, Hyunsuk Peter Suh
Summary: This study aimed to explore the application of an ultrathin anterolateral thigh (ALT) flap harvested through the superficial fat layer for reconstructing shallow defects. The results showed that the ultrathin ALT flap can achieve optimal reconstruction of thin body areas and is particularly suitable for female patients with thicker thighs and patients with a high body mass index.
PLASTIC AND RECONSTRUCTIVE SURGERY
(2023)
Article
Medicine, General & Internal
Mauro Vasella, Marco Guidi, Matthias Waldner, Maurizio Calcagni, Pietro Giovanoli, Florian S. Frueh
Summary: ICG fluorescence angiography plays a crucial role in surgical treatment of degloving foot injuries, allowing for precise debridement and skin preservation, positively impacting postoperative healing outcomes.
Article
Endocrinology & Metabolism
Stefan Kotschi, Anna Jung, Nienke Willemsen, Anahita Ofoghi, Bettina Proneth, Marcus Conrad, Alexander Bartelt
Summary: Diminished proteasome function is a new mechanistic feature of ferroptosis. The transcription factor NFE2L1 protects cells from ferroptosis by sustaining proteasomal activity. Loss of NFE2L1 is associated with cellular viability reduction after ferroptosis induction and has been observed in a patient-derived cell line and in Gpx4-deficient mice.
MOLECULAR METABOLISM
(2022)
Article
Multidisciplinary Sciences
Sven Otto, Jule K. Michler, Stefan Dhein, Christoph K. W. Muelling
Summary: Distal axonopathy affects a variety of species, including horses, leading to disorders like recurrent laryngeal neuropathy in equine patients. In this study, equine larynges were examined in a constant pressure perfused setup to test their vitality and functionality, providing a solid basis for further investigations into equine larynx disorders and potentially serving as a model for other species.
Article
Chemistry, Multidisciplinary
Xiang Lin, Bin Kong, Yujuan Zhu, Yuanjin Zhao
Summary: A novel biohybrid scaffold, which integrates anisotropic fish scales and mesenchymal stem cells (MSCs), is proposed for skin flap regeneration. The scaffold promotes cell proliferation and differentiation and exhibits anti-inflammatory properties, leading to improved survival rates of the flaps.
Article
Multidisciplinary Sciences
Muqian Wei, Xi Bu, Guanhuier Wang, Yonghuan Zhen, Xin Yang, Dong Li, Yang An
Summary: This article summarized our 12-year experience in Chinese nasal reconstruction and evaluated the aesthetic and functional effect of expanded forehead flap. Understanding the unique skin type and anatomical features of Chinese patients was crucial in achieving successful multiracial nasal reconstruction. The use of clinical data and a detailed case study supported the strategy of extensive application of expanded forehead flap and flip scar flap for internal lining. Additionally, the use of costal and auricular cartilage was prompted by the characteristics of Chinese patients. Emerging technologies like 3D-printing could bring further advancements in nasal reconstruction.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Naoya Otani, Koichi Tomita, Yuki Kobayashi, Kazuya Kuroda, Yoshihisa Koyama, Hikaru Kobayashi, Tateki Kubo
Summary: This study assessed the effect of a Si-based agent on inhibiting ischemia-reperfusion injury in skin flaps and found that it significantly improved the survival rate of flaps and reduced inflammation, oxidative stress, and apoptosis.
SCIENTIFIC REPORTS
(2022)
Article
Critical Care Medicine
Mickael Tobalem, Reto Wettstein, Elizabeth Tschanz, Jan Plock, Nicole Lindenblatt, Yves Harder, Farid Rezaeian
Editorial Material
Oncology
Elisabeth A. Kappos, Walter P. Weber, Jana de Boniface, Yves Harder, Joerg Heil, Florian Fitzal, Zoltan Matrai
Article
Clinical Neurology
Anke Scheel-Sailer, Nushaba Aliyev, Dominique Jud, Simon Annaheim, Yves Harder, Joerg Krebs, Alessia Wildisen, Reto Wettstein
Article
Oncology
Nicola Rocco, Giacomo Montagna, Rosa Di Micco, John Benson, Carmen Criscitiello, Li Chen, Bruno Di Pace, Antonio Jesus Esgueva Colmenarejo, Yves Harder, Andreas Karakatsanis, Anna Maglia, Marco Mele, Nahid Nafissi, Pedro Santos Ferreira, Wafa Taher, Antonio Tejerina, Alessio Vinci, Maurizio Nava, Giuseppe Catanuto
Summary: The study revealed that different countries and regions are experiencing various phases of the COVID-19 pandemic, leading to different approaches in breast cancer surgery practices. The priority in surgery is given to patients with aggressive disease, while elective surgeries are being restricted as the pandemic worsens.
Article
Chemistry, Multidisciplinary
Francesco Muoio, Stefano Panella, Matias Lindner, Valentin Jossen, Yves Harder, Tiziano Moccetti, Regine Eibl, Michele Mueller, Tiziano Tallone
Summary: The study developed a novel platform based on chemically defined serum-free cell culture medium and biodegradable microcarrier BR44, enabling the growth of human adipose stem cells while maintaining their undifferentiated state. The BR44 microcarrier allowed hASCs to adhere and grow in a chemically defined serum-free medium, showing better undifferentiated status compared to cells cultured on traditional 2D surfaces.
APPLIED SCIENCES-BASEL
(2021)
Article
Cell Biology
Stefano Panella, Francesco Muoio, Valentin Jossen, Yves Harder, Regine Eibl-Schindler, Tiziano Tallone
Summary: Adipose tissue is a rich source of stem cells, but liposuction cannot provide enough for direct applications in regenerative medicine. Hence, GMP-compliant ex vivo expansion protocols are needed to ensure safe, reproducible, and cost-effective production of cell drugs. The developed UrSuppe cell culture medium allows for the cultivation and differentiation of human adipose stem cells, potentially facilitating the translation of research projects into clinical applications.
Article
Surgery
Yves Harder, Johanna Krapf, Bernd Rieck, Dirk J. Schaefer, Gabriel Djedovic, Ingo Kutschka, Karolin Graf, Ulrich Kneser, Raymund E. Horch, Marcus Lehnhardt, Gerhard Pierer
Summary: TSWI is a rare yet potentially life-threatening complication that may occur after median sternotomy, characterized by biofilm formation and requiring early multidisciplinary decision making for optimal patient care. Treatment typically involves repeated surgical wound debridements and wound conditioning until achieving a clean wound status, followed by wound closure with various pedicled and free flaps for infection eradication and defect reconstruction.
HANDCHIRURGIE MIKROCHIRURGIE PLASTISCHE CHIRURGIE
(2021)
Editorial Material
Oncology
Florian Fitzal, Michael Bolliger, Daniela Dunkler, Angelika Geroldinger, Luca Gambone, Jorg Heil, Fabian Riedel, Jana de Boniface, Camilla Andre, Zoltan Matrai, David Pukancsik, Regis R. Paulinelli, Valerijus Ostapenko, Arvydas Burneckis, Andrej Ostapenko, Edvin Ostapenko, Francesco Meani, Yves Harder, Marta Bonollo, Andrea S. M. Alberti, Christoph Tausch, Barbel Papassotiropoulos, Ruth Helfgott, Dietmar Heck, Hans-Jorg Fehrer, Markus Acko, Peter Schrenk, Elisabeth Trapp, Pristauz-Telsnigg Gunda, Paliczek Clara, Giacomo Montagna, Mathilde Ritter, Jens-Uwe Blohmer, Sander Steffen, Laszlo Romics, Elizabeth Morrow, Katharina Lorenz, Mathias Fehr, Walter Paul Weber
ANNALS OF SURGICAL ONCOLOGY
(2022)
Article
Oncology
Florian Fitzal, Michael Bolliger, Daniela Dunkler, Angelika Geroldinger, Luca Gambone, Joerg Heil, Fabian Riedel, Jana de Boniface, Camilla Andre, Zoltan Matrai, David Pukancsik, Regis R. Paulinelli, Valerijus Ostapenko, Arvydas Burneckis, Andrej Ostapenko, Edvin Ostapenko, Francesco Meani, Yves Harder, Marta Bonollo, Andrea S. M. Alberti, Christoph Tausch, Baerbel Papassotiropoulos, Ruth Helfgott, Dietmar Heck, Hans-Joerg Fehrer, Markus Acko, Peter Schrenk, Elisabeth K. Trapp, Pristauz-Telsnigg Gunda, Paliczek Clara, Giacomo Montagna, Mathilde Ritter, Jens-Uwe Blohmer, Sander Steffen, Laszlo Romics, Elizabeth Morrow, Katharina Lorenz, Mathias Fehr, Walter Paul Weber
Summary: Recent data suggest that larger resection volumes in oncoplastic surgery, such as OBCII, may improve local control and reduce reexcision rates significantly compared to conventional breast conserving surgery (BCS) or oncoplastic breast conservation with lower resection volumes (OBCI). Despite differences in tumor size and resection margins, there were no differences in local, regional, or distant recurrence-free or overall survival between patients treated with OBCII and those treated with BCS/OBCI.
ANNALS OF SURGICAL ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Andrea Weinzierl, Yves Harder, Daniel Schmauss, Emmanuel Ampofo, Michael D. Menger, Matthias W. Laschke
Summary: This study directly compared the vascularization capacity and survival of white adipose tissue (WAT) and brown adipose tissue (BAT) grafts. The results showed that BAT grafts had impaired vascularization capacity and survival compared to WAT grafts.
Article
Biochemistry & Molecular Biology
Andrea Weinzierl, Yves Harder, Daniel Schmauss, Michael D. Menger, Matthias W. Laschke
Summary: This study demonstrates that microvascular fragments (MVF) derived from adipose tissue can reduce flap necrosis by increasing angiogenesis, improving tissue perfusion, and suppressing apoptosis. These findings provide a promising strategy for reducing ischemia-induced flap necrosis in clinical practice by injecting MVF.
Article
Nutrition & Dietetics
Andrea Weinzierl, Maximilian Coerper, Yves Harder, Michael D. Menger, Matthias W. Laschke
Summary: This study demonstrates that a 30% reduction in caloric intake effectively prevents flap necrosis by maintaining microperfusion and inhibiting inflammation under ischemic stress.
Article
Medicine, General & Internal
Simone Schiaffino, Andrea Cozzi, Barbara Pompei, Angela Lia Scarano, Carola Catanese, Armin Catic, Lorenzo Rossi, Filippo Del Grande, Yves Harder
Summary: This study evaluates the potential complications and effects of a new MRI-conditional breast tissue expander on the magnetic resonance imaging (MRI) image quality. The study finds that the expander does not affect the image quality of T1-weighted and T2-weighted sequences, but moderately affects the diffusion-weighted imaging (DWI) quality.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Medicine, General & Internal
Elisabeth A. Kappos, Alexandra Schulz, Meredith M. Regan, Giusi Moffa, Yves Harder, Karin Ribi, Shelley Potter, Andrea L. Pusic, Mathias K. Fehr, Lars G. Hemkens, Thomas Holzbach, Jian Farhadi, Colin Simonson, Michael Knauer, Ralph Verstappen, Heiner C. Bucher, Daniel Zwahlen, Frank Zimmermann, Matthias Schwenkglenks, Rosine Mucklow, Jane Shaw, Vesna Bjelic-Radisic, Amelia Chiorescu, Yoon S. Chun, Subrina Farah, Chen Xiaosong, Linda Nigard, Sherko Kuemmel, Roland Reitsamer, Maik Hauschild, Ilario Fulco, Christoph Tausch, Thomas Fischer, Dimitri Sarlos, Mihai A. Constantinescu, Judith E. Lupatsch, Florian Fitzal, Joerg Heil, Zoltan Matrai, Jana de Boniface, Christian Kurzeder, Martin Haug, Walter P. Weber
Summary: This study aims to compare the impact of prepectoral and subpectoral implant-based breast reconstruction surgeries on patients' long-term (24 months) quality of life and physical well-being, as well as differences in safety, patient satisfaction, aesthetic outcomes, and patient burden.
Article
Engineering, Biomedical
Francesco Muoio, Stefano Panella, Valentin Jossen, Matias Lindner, Yves Harder, Michele Muller, Regine Eibl, Tiziano Tallone
Summary: Research shows that cultivating hASCs in defined serum-free conditions on BR44 microcarriers can preserve the quality and undifferentiated status of the cells. This cultivation method results in a gene expression profile more compatible with an undifferentiated status compared to growth under standard planar conditions.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2021)