4.3 Article

Culture-dependent and culture-independent analysis of lactic acid bacteria from Shanxi aged vinegar

Journal

ANNALS OF MICROBIOLOGY
Volume 62, Issue 4, Pages 1825-1830

Publisher

SPRINGER
DOI: 10.1007/s13213-011-0396-1

Keywords

Vinegar; Lactic acid bacteria; Diversity; Culture-dependent; Culture-independent

Funding

  1. Fundamental Research Funds for the Central Universities in China [2011PY041]
  2. National High Technology Research and Development Program of the People's Republic of China (863 Program) [2006AA10Z1A3]
  3. Shanxi aged vinegar Group Co.

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Shanxi aged vinegar, one of the traditional Chinese vinegars, is brewed by spontaneous fermentation with various microorganisms involved. The previous study showed that lactic acid bacteria (LAB) was the main bacteria population during the conversation period of starch to ethanol of the production. Here, culture-dependent and -independent methods were applied to study the diversity of the LAB population in the fermentation process of Shanxi aged vinegar. Isolates with various genotypic characters were screened and selected based on enterobacterial repetitive intergenic consensus/PCR (ERIC/PCR) fingerprinting. PCR-restriction fragment length polymorphism analysis combined with 16S rDNA sequencing identification revealed that seven species including Lactobacillus fermentum, L. plantarum, L. casei, L. buchneri, Pediococcus acidilactici, P. pentosaceus and Weissella confusa were recovered. DNA extracted directly from fermenting samples was separated by the PCR-denaturing gradient gel electrophoresis, which indicated that the main species presented in the period of starch changed into ethanol were L. fermentum, Leuconostoc mesenteroides and W. confusa.

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