Journal
ANNALS OF MICROBIOLOGY
Volume 59, Issue 3, Pages 399-408Publisher
INST MICROBIOLOGIA
DOI: 10.1007/BF03175122
Keywords
wastewater stabilisation ponds; purple anoxygenic phototrophs; pufM gene; stratification; T-RFLP
Categories
Funding
- Ministry of High Education, Scientific Research and Technology
Ask authors/readers for more resources
Both eutrophication and thermal stratification lead to degradation of wastewater treatment efficiency and have a major effect on the wastewater pond biology, but their effects on phototrophic anoxygenic bacterial community is not as well understood. Terminal restriction fragment length polymorphism analysis proved to be a valuable technique that could resolve the diversity and shift of the purple anoxygenic phototrophic community composition in three stage wastewater stabilization ponds (WSP) exhibiting periodically red water phenomenon. Chemical and biological parameters confirmed the eutrophic state during the appearance of the red water. Concomitantly a decrease of ponds performances is reported with total removal percentage of 27, 36 and 43% for Total suspended solid (TSS), DBO(5) and DCO, respectively. By targeting the pufM gene, 74 Terminal restriction fragments (TRFs) were detected in the three studied ponds which 78% were located in the anaerobic and facultative ponds. Simpson (D) and Shannon (H') diversity index showed a loss of phototrophic bacterial diversity from the anaerobic to the maturation pond, especially in the water phase. Principal coordinate analysis (PCoA) of both HpaII and HaeIII - T-RFLP profiles, allowed deducting a differential distribution between the water and sediments samples.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available