Journal
ANNALS OF ALLERGY ASTHMA & IMMUNOLOGY
Volume 109, Issue 1, Pages 52-58Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.anai.2012.05.017
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Funding
- Fondo de Investigacion Sanitaria [PI061561, PS09/00966]
- Consejeria de Salud
- Junta de Andalucia [PI-0542-2010]
- Ministerio de Ciencia e Innovacion project [BIO2009-07050]
- Ministrsterio de Economia
- Innovacion y Ciencia [CTS-7433]
- FIS-Thematic Networks and Cooperative Research Centers: RIRAAF [RD07/0064]
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Background: Pru p 3 is the major peach allergen and the most frequent cause of food allergy in adults in the Mediterranean area. Although its allergenicity is well characterized, its ability to generate a T-cell response is not completely known. Objective: To investigate the influence of Pru p 3 allergen on dendritic cell (DC) maturation and specific T-cell response (T(H)1/T(H)2) in peach allergic patients. Methods: Peach allergic patients (n = 11) and tolerant controls (n = 14) were included in the study. Monocyte-derived DC maturation after incubation with Pru p 3 was evaluated by the increase of maturational markers (CD80, CD86, and CD83) by flow cytometry. Lymphocyte proliferation was evaluated by coculturing monocyte-derived DCs and 5,6-carboxyfluorescein diacetate N-succinimidyl ester-stained lymphocytes with different concentrations of Pru p 3 (25, 10, and 1 mu g/mL) by flow cytometry and cytokine production. Results: Pru p 3 induced a significant increase in the CD80, CD86, and CD83 expression on stimulated DCs from patients compared with controls. The lymphocyte proliferative response after Pru p 3 stimulation was also significantly higher along with an increase in interleukin 8 in patients compared with tolerant controls. Conclusion: Pru p 3 allergen induces changes in DC maturational status mainly in peach allergic patients. An increase in lymphocyte proliferative response accompanied with a different cytokine pattern was also observed compared with healthy controls. (C) 2012 American College of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.
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