4.7 Article

Rapid, simple and inexpensive production of custom 3D printed equipment for large-volume fluorescence microscopy

Journal

INTERNATIONAL JOURNAL OF PHARMACEUTICS
Volume 494, Issue 2, Pages 651-656

Publisher

ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijpharm.2015.03.042

Keywords

3D printing; Additive manufacturing; Optical clearing; CLARITY

Funding

  1. Biomedical Research Centre (Mental Health) at King's College London
  2. Sackler Institute for Translational Neurodevelopment at King's College London
  3. UCL Faculty of Life Sciences
  4. School of Life and Medical Sciences at UCL
  5. King's Bioscience Institute
  6. Guy's and St. Thomas' Charity Prize PhD Programme in Biomedical and Translational Science
  7. MRC [G0901899] Funding Source: UKRI
  8. Medical Research Council [G0901899] Funding Source: researchfish

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The cost of 3D printing has reduced dramatically over the last few years and is now within reach of many scientific laboratories. This work presents an example of how 3D printing can be applied to the development of custom laboratory equipment that is specifically adapted for use with the novel brain tissue clearing technique, CLARITY. A simple, freely available online software tool was used, along with consumer-grade equipment, to produce a brain slicing chamber and a combined antibody staining and imaging chamber. Using standard 3D printers we were able to produce research-grade parts in an iterative manner at a fraction of the cost of commercial equipment. 3D printing provides a reproducible, flexible, simple and cost-effective method for researchers to produce the equipment needed to quickly adopt new methods. (C) 2015 The Authors. Published by Elsevier B.V.

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