Article
Cell Biology
Zhewen Hu, Yiming Chen, Min Gao, Xiaopei Chi, Ying He, Chenguang Zhang, Yue Yang, Yuman Li, Yan Lv, Ying Huang, Xuliang Deng
Summary: This study developed a modified method for the isolation of primary epithelial cells, which showed better cell viability, morphology, and stemness. The modified method also allowed for stable passaging of the cells and facilitated organoid formation. Furthermore, the modified method outperformed the conventional method in isolating different types of epithelial cells.
CELL PROLIFERATION
(2023)
Review
Cell Biology
Zuzanna Bukowy-Bieryllo
Summary: Human airway epithelial (HAE) cellular models are widely used in respiratory research, with the conditionally reprogrammed cell (CRC) models showing promising results for longer in vitro proliferation of HAE cells. The limitations of primary HAE models due to their limited proliferative lifespan have led to the exploration of CRC HAE cultures as a useful alternative.
CELL COMMUNICATION AND SIGNALING
(2021)
Article
Environmental Sciences
Gurucharan Sudarshan, Simy Weil, Weronika Jasinska, Rivka Manor, Oron Goldstein, Eliahu D. Aflalo, Rivka Ofir, Sean V. Zimin, Tom Levy, Benyamin Rosental, Yariv Brotman, Amir Sagi
Summary: This study utilizes mass spectrometry to analyze lipid content changes in prawn culture media and investigates the correlation between specific metabolites in media and cell cycle and proliferation status. The results show that different media have varying effects on cellular metabolic activity and cell count, with Opti-MEM being the most optimal for short-term culture. Additionally, lipids such as phosphatidylcholine and phosphatidylserine are consistently abundant in all three media and palmitate levels correlate with proliferation phase.
FRONTIERS IN MARINE SCIENCE
(2023)
Article
Immunology
Jingxia Gao, Guanzhong Song, Haibo Shen, Yiming Wu, Chongqi Zhao, Zhuo Zhang, Qian Jiang, Xilong Li, Xiaokang Ma, Bie Tan, Yulong Yin
Summary: This study found that allicin improves intestinal barrier function and prevents damage caused by bacteria. It increases cell viability and enhances the integrity of cell monolayers.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Sheng Yang, Jingxian Wu, Xuemei Xian, Qiusheng Chen
Summary: This study reports an efficient and simple method for duck neuron culture. By exploring the development timing of neurons and astrocytes during duck embryonic stage, the optimal sampling age for neuron culture was determined. The brain tissue isolation method (papain digestion) and cell suspension inoculation density (7×10^5 cells/mL) were identified as the culture protocol, which resulted in high viability and density of target cells. The purity of cultured neurons was over 95%.
Article
Multidisciplinary Sciences
Chithra K. Muraleedharan, Jay Mierzwiak, Darius Feier, Asma Nusrat, Miguel Quiros
Summary: The intestinal epithelium, as a single layer of cells, plays a crucial role in barrier function. While traditional cell models from carcinomas have limitations, the emergence of 3D enteroids has provided new opportunities for primary cell culture, though maturity issues remain.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Review
Biochemistry & Molecular Biology
Phuong A. Ngo, Markus F. Neurath, Rocio Lopez-Posadas
Summary: The gut barrier is the body's first line of defense and is crucial for nutrition and immunoregulation. Dysregulated cell shedding is associated with infection, inflammation, and cancer. Investigating the mechanisms controlling cell extrusion is important for understanding intestinal homeostasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Jaakko Saari, Fatima Siddique, Sanna Korpela, Elina Mantyla, Teemu O. Ihalainen, Katri Kaukinen, Katriina Aalto-Setala, Katri Lindfors, Kati Juuti-Uusitalo
Summary: The small intestinal epithelium plays a crucial role in nutrition, drug absorption, and metabolism. By using animal-free components and serum-free method, functional small intestinal epithelial cells can be generated from iPSCs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Erzsebet Paszti-Gere, Judit Pomothy, Akos Jerzsele, Oliver Pilgram, Torsten Steinmetzer
Summary: The study demonstrated that MI-1900 and MI-1907 were tolerated up to 50 μM in HIEC-6 and PHH, without affecting cell viability, occludin distribution or inflammatory cytokine levels. However, redox status in PHH was disturbed by the inhibitors. Additionally, MI-1900 was shown to inhibit the replication and spread of SARS-CoV-2 in infected Calu-3 cells by targeting the host protease TMPRSS2.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2021)
Review
Immunology
Shahram Solaymani-Mohammadi
Summary: Human giardiasis is a common parasitic disease that can lead to malnutrition and developmental disorders. Understanding the interaction between the parasite and the intestinal epithelial cells may provide insights into the pathology and help identify new therapeutic approaches.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Panagiota Kakni, Carmen Lopez-Iglesias, Roman Truckenmueller, Pamela Habibovic, Stefan Giselbrecht
Summary: The inner surface of the intestine is a dynamic system. Researchers have developed intestinal organoids that mimic intestinal architecture and cell composition. However, the current models limit the study of the apical side of the epithelium. This study presents a novel intestinal organoid model with reversed polarity, which provides a new research tool for studying nutrient/drug uptake, metabolism, and host-microbiome/pathogen interactions.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yu Yu, Wenjing Yang, Anthony J. Bilotta, Xiaojing Zhao, Yingzi Cong, Yanqing Li
Summary: The study found that host-derived L-lactate can promote intestinal epithelial cell (IEC) migration by increasing mitochondrial ATP production in IEC, aiding in promoting intestinal wound healing. Additionally, administering mice with L-lactate can suppress colitis induced by dextran sulfate sodium.
Article
Cell Biology
Kristen L. Segars, Nicholas A. Azzari, Stephanie Gomez, Cody Machen, Celeste B. Rich, Vickery Trinkaus-Randall
Summary: Research has shown that changes in corneal stiffness and signaling contribute to decreased wound healing efficacy in older mice. As mice age, the stiffness of the cornea changes, leading to longer healing times. Additionally, there is reduced calcium signaling at the wound site in older mice, resulting in lower cell activity compared to younger mice.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Gastroenterology & Hepatology
Caihan Duan, Junhao Wu, Zhe Wang, Chen Tan, Lingzhi Hou, Wei Qian, Chaoqun Han, Xiaohua Hou
Summary: The study found that fucose can accelerate the proliferation and differentiation of intestinal stem cells (ISCs), which is related to gut microbiota and metabolism. Fucose promotes Akkermansia-related propanoate metabolism to accelerate ISC-mediated intestinal epithelial development, and this effect is achieved through activation of the Wnt signaling pathway. These findings provide new insights into the promotion of gut homeostasis and the potential application of fucose as a prebiotic.
Article
Immunology
Ye Seul Son, Naeun Son, Won Dong Yu, Aruem Baek, Young-Jun Park, Moo-Seung Lee, Seon-Jin Lee, Dae-Soo Kim, Mi-Young Son
Summary: This study investigated the effects of PM10 on inflamed intestines using two-dimensional and three-dimensional human intestinal models. The results showed that PM10 exposure led to pathological changes in the intestinal epithelial cells, exacerbating inflammatory disorders.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
H. Yu, M. M. Azzam, Y. B. Wang, X. J. Lin, A. H. Alqhtani, A. A. Al-Abdullatif, I. A. Alhidary, S. Q. Jiang
Summary: The experiment aimed to estimate the dietary requirements of sodium and chloride for Lingnan yellow chickens aged 43 to 63 days. The results indicated that supplementing NaCl improved body gain, feed efficiency, and water intake, while affecting blood serum levels and meat quality. By using a quadratic broken-line regression model, the optimal dietary sodium and chloride levels were determined to be 0.09%, 0.10%, and 0.10% for various physiological parameters in slow-growing broiler breeds.
JOURNAL OF APPLIED POULTRY RESEARCH
(2022)
Article
Food Science & Technology
Mona A. Hassan, Azza M. A. Abo-Elmaaty, Asmaa W. Zaglool, Sally A. M. Mohamed, Shimaa M. Abou-Zeid, Mayada R. Farag, Mahmoud Alagawany, Alessandro Di Cerbo, Mahmoud M. Azzam, Rashed Alhotan, Enas EL-Hady
Summary: The current study investigated the toxic effects of AFB1 on rabbit kidneys and the therapeutic role of O. vulgare essential oil (OEO) in reducing these effects. Results showed that OEO decreased cystatin C levels in the AFB1 group and reduced oxidative stress and lipid peroxidation in the co-exposed group. OEO also reduced DNA damage, inflammatory response, and down-regulated stress and inflammatory cytokines-encoding genes, while preserving the cytoarchitecture of rabbits' kidneys treated with AFB1.
Review
Immunology
Nahed A. El-Shall, Shouqun Jiang, Mayada R. Farag, Mahmoud Azzam, Abdulaziz A. Al-Abdullatif, Rashed Alhotan, Kuldeep Dhama, Faiz-ul Hassan, Mahmoud Alagawany
Summary: The increase in drug resistance and ineffective immunization against pathogens pose a significant threat to the poultry industry. Spirulina, a natural ingredient rich in nutrients and antioxidants, can beneficially affect gut health and disease resistance in chickens. It has antibacterial effects and can also exhibit antiviral activities against common human or animal viruses. Additionally, Spirulina can modulate the immune system, improving disease resistance and growth rates in poultry. This review discusses the potential use of Spirulina as a dietary supplement to enhance growth, gut health, and disease resistance in poultry.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Food Science & Technology
Abdulaziz Al-Abdullatif, Elsayed Hussein, Gamaleldin Suliman, Musab Akasha, Mohammed Al-Badwi, Hatem Ali, Mahmoud Azzam
Summary: This study found that replacing palm oil with rice bran oil as a dietary energy source can improve the nutritional properties and fatty acid deposition in broiler chickens. The use of rice bran oil improves feed conversion ratio and enhances meat quality. Additionally, the inclusion of rice bran oil in the diet alters the fatty acid composition of chicken meat, increasing the deposition of omega-3 fatty acids.
Article
Fisheries
Mayada R. Farag, Chuntian Zheng, Heba S. A. Gharib, Enas EL-Hady, Eman A. A. Mahdy, Azza M. A. Abo-Elmaaty, Shimaa M. Abou-Zeid, Mahmoud Alagawany, Alessandro Di Cerbo, Mahmoud M. Azzam, Abdulaziz A. Al-Abdullatif, Mona A. Hassan
Summary: This study aimed to investigate the harmful effects of nanoparticles on Nile tilapia fish and the potential benefits of chamomile essential oil in mitigating these effects. The findings revealed neurobehavioral changes, alterations in brain neurotransmitters, and reduced enzyme activities in fish exposed to nanoparticles. Furthermore, supplementation of chamomile essential oil significantly reduced the negative impacts of nanoparticles.
AQUACULTURE NUTRITION
(2023)
Article
Marine & Freshwater Biology
Samah R. Khalil, Chuntian Zheng, Shimaa M. Abou-Zeid, Mayada R. Farag, Hesham S. Elsabbagh, Mouhamed S. Siddique, Mahmoud M. Azzam, Alessandro Di Cerbo, Basma A. Elkhadrawey
Summary: Zinc oxide nanoparticles (ZnO-NPs) have many exciting properties, but their accumulation in aquatic ecosystems and fish exposure can have deleterious effects. Thymol shows potential in counteracting the immunotoxic effects of ZnO-NPs.
AQUATIC TOXICOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Mahmoud Mostafa Azzam, Abdulaziz Al-Abdullatif, Musab Akasha, Rashed Alhotan, Gamaleldin Suliman, Ludovic Lahaye, Elizabeth Santin
Summary: One-day-old male broiler chickens (Ross 308) were assigned to 3 dietary treatments to investigate the effects of the protected complex of biofactors and anti-oxidants [P(BF+AOX)] on their growth performance, meat quality, and organ weight. The results showed that the addition of 150 mg/kg of P(BF+AOX) decreased the relative weight of liver, heart, and spleen, while the addition of 300 mg/kg of P(BF+AOX) decreased the serum level of uric acid. The study suggests that P(BF+AOX) can be considered as a functional nutrient in broiler diets up to a concentration of 150 mg/kg.
Article
Agriculture, Dairy & Animal Science
Mayada R. Farag, Claudia Zizzadoro, Mahmoud Alagawany, Shimaa M. Abou-Zeid, Suzan Attia Mawed, Mohamed S. El Kholy, Alessandro Di Cerbo, Mahmoud M. Azzam, Eman A. A. Mahdy, Mariam H. E. Khedr, Walaa M. Elhady
Summary: Thiacloprid (TH) is an insecticide used in agriculture to protect fruits and vegetables from insects, but it has deleterious effects on non-target organisms. This study investigated the protective effects of Chicoric (CA) and rosmarinic (RA) acids on chick embryos exposed to TH. The results showed that CA and RA can mitigate the toxic effects of TH on hatchlings.
Article
Biochemistry & Molecular Biology
Mayada R. Farag, Haitham G. Abo-Al-Ela, Mahmoud Alagawany, Mahmoud M. Azzam, Mohamed T. El-Saadony, Stefano Rea, Alessandro Di Cerbo, Doaa S. Nouh
Summary: Recently, research has focused on the use of silver nanoparticles (AgNPs) as antibacterial agents, but their extensive use has led to water pollution and harmful effects on aquatic organisms. This study investigated the potential mitigative effect of quercetin nanoparticles (QNPs) against AgNPs-induced toxicity in Nile tilapia. The results showed that AgNPs caused oxidative damage and hepatotoxic effects, while QNPs enhanced and restored the physiological parameters and health status of the fish.
Article
Biology
Abdulaziz Al-Abdullatif, Mahmoud Mostafa Azzam
Summary: This study aimed to investigate the effects of heat stress on the growth performance, carcass traits, and nutritional composition of breast meat in broiler chickens under hot arid environments. The results showed that heat stress resulted in decreased live body weight, weight gain, and feed intake, as well as increased hot and cold carcass yields. The relative heart and liver weights decreased, while wing meat yields increased in heat-stressed broiler chickens. The shrinkage of the carcass percentage increased during chilling. However, there were no significant differences in the ultimate pH values, cooking loss, and contents of moisture, crude protein, and fat of breast meat between the thermoneutral and heat stress groups.
Article
Agriculture, Dairy & Animal Science
Aisha Zaki, Shouqun Jiang, Saad Zaghloul, Talaat K. El-Rayes, Ahmed A. Saleh, Mahmoud Mostafa Azzam, Marco Ragni, Mahmoud Alagawany
Summary: This research evaluated the effects of using betaine as a substitute for choline on the productive performance, egg quality parameters, fatty acids profile, and antioxidant status in laying hens. The results showed that replacing choline with betaine did not significantly affect body weight, egg production, and feed intake. However, egg weight and egg mass increased, and yolk color improved in the group supplemented with betaine. The levels of various blood parameters, liver malondialdehyde content, yolk vitamin E, and fatty acid levels were not significantly affected by betaine supplementation. Additionally, hens fed betaine displayed an increased antibody titer for Newcastle disease virus.
Article
Agriculture, Dairy & Animal Science
Gamaleldin M. Suliman, Abdullah N. Al-Owaimer, Ayman A. Swelum, Rashed Alhotan, Mohammed M. Qaid, Mahmoud M. Azzam, Elsayed O. S. Hussein
Summary: This study investigated the chemical composition, amino acids and fatty acids profiles, and health and nutrition values of ROSS 308 broiler chicken breast muscle at different slaughter age. The results showed that slaughter age significantly influenced the levels of certain amino acids and fatty acids, as well as health indices. There was no significant difference between male and female chickens, except for stearidonic acid levels. This study provides valuable insights into the nutritional value of meat from fast-growing broiler chickens.
Article
Agriculture, Dairy & Animal Science
Hani H. Al-Baadani, Rashed A. Alhotan, Mahmoud M. Azzam
Summary: The present study investigated the effects of gum arabic as a prebiotic and different stocking densities on broiler chickens. The results showed that gum arabic improved the growth performance and intestinal morphology of the chickens, while high stocking density negatively affected their performance, blood indices, carcass characteristics, and intestinal morphology.
Article
Food Science & Technology
Hani H. Al-Baadani, Rashed A. Alhotan, Mahmoud M. Azzam, Gamaleldin M. Suliman, Abdulrahman S. Alharthi, Esam H. Fazea, Ibrahim A. Alhidary
Summary: This study showed that gum Arabic has positive effects on the growth performance and some quality indicators of broiler breast meat, especially at the level of 0.12%. However, it did not affect the carcass characteristics and physical indicators of breast meat.
Article
Microbiology
Sheng Zhang, Qin Wang, Jinling Ye, Qiuli Fan, Xiajing Lin, Zhongyong Gou, Mahmoud M. Azzam, Yibing Wang, Shouqun Jiang
Summary: This study investigated the effects of bilberry anthocyanin (BA) on immune function in chickens infected with Salmonella Typhimurium (S. Typhimurium). The results showed that dietary BA alleviated weight loss and improved jejunal morphology caused by S. Typhimurium infection. BA also regulated immune-related genes and proteins, as well as signaling pathways associated with immune response.
FRONTIERS IN MICROBIOLOGY
(2023)
