Journal
ANIMAL REPRODUCTION SCIENCE
Volume 132, Issue 3-4, Pages 159-168Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.anireprosci.2012.05.017
Keywords
Caprine; Folliculogenesis; FSH; GH; Preantral follicle
Funding
- National Council for Scientific and Technological Development (CNPq, Brazil) [554812/2006-1]
- Brazilian Innovation Agency (FINEP)
- CNPq (Brazil)
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A sequential medium was evaluated on the survival, activation and growth rates of caprine preantral follicles submitted to a long-term culture period, aiming to establish an ideal in vitro culture system. Ovarian fragments were cultured for 16 days in alpha-MEM+ alone or supplemented with hormones (GH and/or FSH) added sequentially on different days of culture. Ovarian fragments were cultured in the first (days 0-8) and second (days 8-16) halves of the culture period, generating 10 treatments: alpha-MEM+/alpha-MEM+, FSH/FSH, FSH/GH, FSH/FSH + GH, GH/GH, GH/FSH, GH/FSH + GH, FSH + GH/FSH + GH, FSH + GH/FSH and FSH + GH/GH. Follicle morphology, viability and ultrastructure were analyzed. After day 1 of culture. FSH treatments maintained the percentage of normal follicles similar to the fresh control. At day 16 of culture, the treatment FSH/GH showed the highest (P< 0.05) percentage of normal follicles. The ultrastructure of follicles was preserved in the fresh control and FSH/GH treatment. Follicles cultured with FSH/GH had a higher (P< 0.05) viability than alpha-MEM+; however the viability was lower (P< 0.05) when compared to the fresh control. The FSH/GH treatment showed the highest (P< 0.05) percentage of follicular activation and secondary follicle formation and produced the largest (P< 0.05) mean follicular diameter after 16 days of culture. In conclusion, a sequential medium supplemented with FSH followed by GH during a long-term culture maintains the survival, viability and ultrastructure of goat preantral follicles, and promotes activation and secondary follicles. (C) 2012 Elsevier B.V. All rights reserved.
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