Article
Reproductive Biology
Ewa Mlyczynska, Patrycja Kurowska, Dominika Wachowska, Malgorzata Grzesiak, Joelle Dupont, Agnieszka Rak
Summary: This study investigated the expression of phoenixin-14 in porcine corpus luteum and its impact on endocrine function. The results showed that phoenixin-14 can regulate the secretion of progesterone and estradiol and affect the expression of hormone receptors in luteal cells. Moreover, G protein-coupled receptor 173 and extracellular signal-regulated kinase 1/2 play important roles in the regulation of endocrine function in luteal cells.
BIOLOGY OF REPRODUCTION
(2023)
Article
Agriculture, Dairy & Animal Science
Cecily Bishop, Vimal Selvaraj, David H. Townson, Joy L. Pate, Milo C. Wiltbank
Summary: The corpus luteum (CL) forms from the cellular remnants of the follicle and produces progesterone required for successful pregnancy. The CL and the embryo have a symbiotic relationship, both relying on each other for survival. The Northeast Multistate Project has contributed to our understanding of the regulation and function of the CL in cattle, providing valuable insights for improving fertility.
JOURNAL OF ANIMAL SCIENCE
(2022)
Article
Cell Biology
Renata Santos Silva, Ana Paula Mattoso Miskulin Cardoso, Ines Cristina Giometti, Loren D'Aprile, Francislaine Anelize Garcia Santos, Arnaldo Shindi Maruyama, Liza Margareth Medeiros de Carvalho Sousa, Suraj Unniappan, Mariusz P. Kowalewski, Paula de Carvalho Papa
Summary: Our study demonstrates that insulin increases the expression of HSD3B and CYP19A1 in canine luteal cells via the MAPK and PI3K pathways, leading to the regulation of steroid hormone production, specifically progesterone (P4) and 17b-estradiol (E2).
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Pawel Likszo, Dariusz Jan Skarzynski, Beenu Moza Jalali
Summary: This study utilized proteomics and functional analysis to identify changes in key proteins and pathways during different stages of corpus luteum (CL) development, highlighting the importance of pathways such as cell survival, endocytosis, oxidative stress response, estradiol metabolism, and angiogenesis in CL function maintenance. Conversely, changes in protein abundances during CL regression were associated with decreased steroid synthesis, increased apoptosis and immune cell infiltration. Pregnancy establishment rescued CL by maintaining expression of proteins supporting steroidogenesis with enrichment of pathways such as cholesterol biosynthesis and functions like fatty acid metabolism in CL of pregnancy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Jaya Bharati, N. H. Mohan, Satish Kumar, Jayashree Gogoi, Sai Kumar, Bosco Jose, Meeti Punetha, Sanjib Borah, Amit Kumar, Mihir Sarkar
Summary: RNA-Seq was used to identify differentially expressed genes (DEGs) in porcine corpus luteum (CL) during different physiological stages, revealing significant enrichment of gene functions in early, mid, and late stages. Apoptosis was most active in the regressed stage. Pathway analysis indicated lipid metabolism, translation, immune, and endocrine system pathways were associated with CL function. Gene network analysis identified novel hub genes AR, FOS, CDKN1A potentially regulating CL physiology.
Article
Agriculture, Dairy & Animal Science
Sarah N. Carr, Benjamin R. Crites, Joy L. Pate, Camilla H. K. Hughes, James C. Matthews, Phillip J. Bridges
Summary: In regions with selenium (Se) deficient soils, supplementing this mineral to the diet of forage-grazing cattle is important. The form of Se supplemented can affect the expression of mRNA encoding selenoproteins in the corpus luteum, leading to changes in progesterone levels. The increase in progesterone appears to be due to increased cholesterol availability by the corpus luteum, not by an increase in the expression of key steroidogenic enzymes.
Review
Developmental Biology
Kayhan Yakin, Francesko Hela, Ozgur Oktem
Summary: The corpus luteum is the main source of progesterone, and understanding its signaling pathways is crucial for managing reproductive disorders. This review focuses on the complex mechanisms of progesterone-induced signaling in luteal granulosa cell activity and discusses future research priorities and limitations of the current data.
MOLECULAR HUMAN REPRODUCTION
(2023)
Article
Food Science & Technology
Dainan Yu, Xun Jiang, Wenjing Ge, Bo Qiao, Dalei Zhang, Hui Liu, Haibin Kuang
Summary: This study found that acrylamide exposure during early- and mid-pregnancy had negative effects on luteal function, including decreased levels of serum progesterone and estradiol, reduced numbers and relative areas of ovarian corpora lutea, dysregulation of ovarian angiogenesis, increased oxidative stress, and induced apoptosis of ovarian cells.
FOOD AND CHEMICAL TOXICOLOGY
(2022)
Review
Agriculture, Dairy & Animal Science
J. L. Pate, C. H. K. Hughes
Summary: The corpus luteum plays a critical role in pregnancy in all mammals. However, the fate of the corpus luteum in ruminants depends on the presence of a functional uterus or signals from a developing embryo. Prostaglandin F2alpha, secreted by the uterus, is a key molecule that needs to be modified. The sensitivity of the corpus luteum to prostaglandin F2alpha can be influenced by internal mechanisms. Understanding the molecular changes that occur during different stages of luteal development and pregnancy can provide insight into the regulation of luteolytic capacity in ruminants.
Article
Reproductive Biology
M. Florencia Gallelli, Carolina Bianchi, Enzo Zampini, Mariana Bertuzzi, Marcelo Aba, Deborah Neild, Marcelo Miragaya
Summary: The administration of hCG on Day 7 post-mating in llamas was found to be effective in developing an accessory corpus luteum (CL) and increasing plasma progesterone (P4) concentration. This treatment had no impact on the original corpus luteum in pregnant llamas, but resulted in a larger corpus luteum diameter in treated non-pregnant llamas. The accessory CL was detected throughout the study in both pregnant and non-pregnant llamas, but its vascularization started to decrease around Day 16 post-mating in non-pregnant animals.
Review
Medicine, General & Internal
Zukaa Almahloul, Bedayah Amro, Zuhdi Nagshabandi, Iman Alkiumi, Zeinabs Hakim, Arnaud Wattiez, Muna Tahlak, Philippe R. R. Koninckx
Summary: Ovarian pregnancy is a rare condition with no established pathophysiology, diagnosis, and treatment. A review of case reports published on PubMed revealed that 8% of ovarian pregnancies occur in women with blocked oviducts and 23% are located on the other side of the corpus luteum. Abdominal bleeding should raise suspicion of ovarian pregnancy due to non-specific symptoms. Surgical excision is the preferred treatment, but caution must be taken not to interrupt an associated intra-uterine pregnancy.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Agriculture, Dairy & Animal Science
Bartlomiej Maria Jaskowski, Hartwig Bostedt, Marek Gehrke, Jedrzej Maria Jaskowski
Summary: The study shows that recipients with a cavitary corpus luteum have higher progesterone levels and pregnancy rates during embryo transfer. Therefore, the presence of a cavity inside the corpus luteum should not be a reason to exclude recipients from embryo transfer.
Review
Veterinary Sciences
Fang Chen, Yi'an Hou, Xiaoqing Zhu, Cheng Mei, Rihong Guo, Zhendan Shi
Summary: In recent decades, embryo transfer has been widely used in dairy cows, but improving the conception rate after transfer remains a major challenge. This review conducted a meta-analysis to evaluate the efficacy of hCG and GnRH in improving pregnancy rates after transfer. The results showed that hCG and GnRH can improve pregnancy rates in cows with poor fertility, but not in cows with good fertility.
VETERINARY SCIENCES
(2023)
Article
Reproductive Biology
Jing Wang, Tianqi Zhu, Xiao Ma, Yehua Wang, Jiping Liu, Guangdong Li, Yunjie Liu, Pengyun Ji, Zhenzhen Zhang, Lu Zhang, Guoshi Liu
Summary: The study reveals strong expression of melatonin synthetic enzyme and MT2 genes in the corpus luteum of mammals, where KO MT2 significantly affects reproductive performance. Melatonin treatment enhances progesterone production and improves corpus luteum function, mediated by MT2.
BIOLOGY OF REPRODUCTION
(2021)
Article
Reproductive Biology
Megan A. Mezera, Wenli Li, Milo C. Wiltbank
Summary: The study found significant alterations in expression of interferon-stimulated genes (ISGs) during early pregnancy, with immune/interferon signaling pathways being enriched. Most ISGs decreased during the second month of pregnancy, but some remained elevated even after cessation of IFNT secretion. Additionally, the study highlighted the importance of lack of PGF exposure in corpus luteum maintenance during early pregnancy, while the roles of diverse ISGs and other pathways activated during early pregnancy remain undefined.
BIOLOGY OF REPRODUCTION
(2021)
Article
Agriculture, Dairy & Animal Science
Ozlem Bedir, Aykut Gram, Sheri T. Dorsam, Anna T. Grazul-Bilska, Mariusz P. Kowalewski
Summary: This study demonstrates the effects of FSH-mediated ovarian hyperstimulation on the caruncles of ewes under various feeding regimes. The findings indicate that the day of the estrous cycle affected the expression of certain genes, while feeding and superovulation had modulatory effects on gene expression levels and responsiveness to superovulation. The data highlight the potential impacts of dietary and hormonal stimuli on uterine physiology, which could ultimately affect pregnancy outcomes.
DOMESTIC ANIMAL ENDOCRINOLOGY
(2022)
Article
Endocrinology & Metabolism
Bettina Lanfranchi, Ricardo Fernandez Rubia, Max Gassmann, Gerhard Schuler, Mariusz P. Kowalewski
Summary: HIF1 alpha regulates the expression of STAR in the ovary, playing a key role in ovarian steroidogenesis.
GENERAL AND COMPARATIVE ENDOCRINOLOGY
(2022)
Article
Cell Biology
Renata Santos Silva, Ana Paula Mattoso Miskulin Cardoso, Ines Cristina Giometti, Loren D'Aprile, Francislaine Anelize Garcia Santos, Arnaldo Shindi Maruyama, Liza Margareth Medeiros de Carvalho Sousa, Suraj Unniappan, Mariusz P. Kowalewski, Paula de Carvalho Papa
Summary: Our study demonstrates that insulin increases the expression of HSD3B and CYP19A1 in canine luteal cells via the MAPK and PI3K pathways, leading to the regulation of steroid hormone production, specifically progesterone (P4) and 17b-estradiol (E2).
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Miguel Tavares Pereira, Paula Papa, Iris Margaret Reichler, Selim Aslan, Mariusz Pawel Kowalewski
Summary: The canine corpus luteum (CL) is the primary source of reproductive steroids in dogs during dioestrus, and remains active even in the absence of pregnancy. Previous studies have shown that estrogen receptors ESR1 and ESR2, as well as regulators of estrogen availability such as HSD17B7, SULT1E1, and STS, are present in non-pregnant canine CL. This study aimed to evaluate the mRNA levels of these factors in the canine CL during non-pregnant dioestrus and different stages of pregnancy. Results showed changes in the availability of ESR1, HSD17B7, SULT1E1, and STS during pregnancy, with significant findings in the non-pregnant dioestrus including alterations in ESR2:ESR1 ratio, STS levels, and HSD17B7 mRNA amounts.
REPRODUCTION IN DOMESTIC ANIMALS
(2022)
Article
Agriculture, Dairy & Animal Science
Ali Kazemian, Miguel Tavares Pereira, Bernd Hoffmann, Mariusz P. Kowalewski
Summary: Adequate embryo-maternal communication is crucial for a successful pregnancy. Progesterone signaling plays a crucial role in modulating decidual cells physiology and biological activity in the dog. The expression of decidualization markers and acquisition of epithelial-like characteristics are important aspects of the functional involvement of progesterone receptor (PGR) in canine decidual cells. These findings provide insights into the maintenance and termination of canine pregnancy.
Article
Reproductive Biology
Ozlem Bedir, Aykut Gram, Anna T. Grazul-Bilska, Mariusz P. Kowalewski
Summary: Disturbances at the conceptus-maternal interface can affect pregnancy outcome, and imbalanced nutrition can alter gene expression and uterine responsiveness to hormonal stimulation.
Article
Agriculture, Dairy & Animal Science
Annette Liesegang, Bettina Burger, Therese de Vries de Heekelingen, Corinne Schroeter-Vogt, Jean-Michel Hatt, Mariusz P. Kowalewski, Marcus Clauss
Summary: Hindgut fermenting herbivores, such as tortoises and certain mammals, absorb more calcium from their diet than they need and excrete the excess calcium through urine. The exact reasons for this mechanism are still unclear, but it may be related to ensuring phosphorus availability for gut microbes by removing calcium. Ussing chamber experiments on rabbits showed that active calcium absorption increased with higher dietary calcium levels, contradicting the assumption that passive uptake would be more prevalent at higher levels. This finding supports the hypothesis of deliberate removal of calcium. However, the distribution of proteins involved in active calcium absorption suggests that other factors not investigated in this study play a major role in rabbits' calcium absorption.
JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION
(2023)
Review
Agriculture, Dairy & Animal Science
Mariusz P. Kowalewski
Summary: Canine pregnancy relies on luteal steroidogenesis for progesterone production. The placenta's response to progesterone depends on the P4 receptor. The unique characteristics of canine placentation make it an interesting model for studying trophoblast invasion regulation. Understanding PGR-mediated signaling is clinically significant for reproductive performance control in dogs.
REPRODUCTION IN DOMESTIC ANIMALS
(2023)
Article
Reproductive Biology
Ali Kazemian, Miguel Tavares Pereira, Selim Aslan, Rita Payan-Carreira, Iris M. Reichler, Reha A. Agaoglu, Mariusz P. Kowalewski
Summary: Up until now, the biological functions of P4 in the dog placenta have been attributed to the maternal stroma-derived decidual cells as the only placental cells expressing the nuclear P4 receptor (PGR). However, it is now suggested that P4 can also exert its effects through membrane-bound receptors. The expression of mPRa, -b, -g, PGRMC1 and -2 in different stages of pregnancy and prepartum luteolysis was investigated to test this hypothesis.
Article
Veterinary Sciences
Valentina Kuczwara, Gerhard Schuler, Christiane Pfarrer, Louiza Tiedje, Ali Kazemian, Miguel Tavares Pereira, Mariusz P. Kowalewski, Karl Klisch
Summary: The study confirmed the presence of contractile myofibroblasts in the maternal placental stroma of cows and identified the co-localization of several factors. It was postulated that these myofibroblasts play an important role in post-partum placental events in cattle. The findings provide a basis for further investigations into the functional aspects and role of myofibroblasts in cattle.
VETERINARY SCIENCES
(2023)
Article
Veterinary Sciences
Miguel Tavares Pereira, Isabelle Martin, Hubert Rehrauer, Mariusz P. Kowalewski, Felicitas S. Boretti, Nadja S. Sieber-Ruckstuhl
Summary: This study evaluated the transcriptomic effects of chronic hypercortisolism on visceral adipose tissue (VAT) in dogs and found that prolonged hypercortisolism affects various regulatory mechanisms in VAT. Treatment also modulated local glucocorticoid and insulin signaling and hormonal sensitivity, suggesting potential targets for further clinical studies. Similar effects of chronic hypercortisolism on VAT were observed in both dogs and humans.
VETERINARY SCIENCES
(2022)
Article
Veterinary Sciences
Aykut Gram, Mariusz P. Kowalewski
Summary: Escherichia coli is a common bacterium that causes infertility in livestock by inducing inflammation and disrupting angiogenesis and vascular function in the uterus and mammary gland. The study demonstrates that LPS, a component of E. coli, triggers inflammatory reactions in endothelial cells through different signaling pathways involving TLR2 and TLR4, leading to the altered expression of proinflammatory cytokines.
VETERINARY SCIENCES
(2022)
Meeting Abstract
Agriculture, Dairy & Animal Science
M. Tavares Pereira, R. Renata Nowaczyk, Rita Payan-Carreira, Miranda Sonia, Selim Aslan, Serhan S. Ay, Mariusz P. Kowalewski
REPRODUCTION IN DOMESTIC ANIMALS
(2021)
Meeting Abstract
Agriculture, Dairy & Animal Science
T. Pereira, P. Papa, I. Reichler, M. P. Kowalewski
REPRODUCTION IN DOMESTIC ANIMALS
(2021)
Meeting Abstract
Agriculture, Dairy & Animal Science
A. Kazemian, M. Tavares Pereira, M. P. Kowalewski
REPRODUCTION IN DOMESTIC ANIMALS
(2021)