Article
Fisheries
Yu Zhang, Xiaowei Fang, Xueyang Chen, Houkun Wang, Jing Liu, Xiongyan Liang, Yufang Gu, Chun Fang, Yuying Yang
Summary: The soft-shelled turtle, an important aquacultural species in China, has been suffering from a bacterial infection caused by Bacillus cereus. This pathogen led to significant pathological changes in infected turtles, and the bacterium showed sensitivity to certain antibiotics, which could be used for prevention and treatment.
Article
Microbiology
Xiaowei Hu, Zidong Xiao, Bo Li, Mingyang Xue, Nan Jiang, Yuding Fan, Peng Chen, Feng Qi, Xianghui Kong, Yong Zhou
Summary: Aeromonas veronii is a gram-negative bacterium that can be found in many aquatic environments and is capable of infecting various aquatic organisms. In this study, we isolated a bacterium named XC-1908 from the liver of Chinese soft-shelled turtles, which was identified as A. veronii based on its characteristics and genetic analysis. The bacterium was pathogenic for the turtles, causing symptoms and histopathological changes consistent with natural infections. Antibiotic sensitivity tests showed that the bacterium was sensitive to certain antibiotics while resistant to others. This study provides strategies for preventing outbreaks of A. veronii infection in Chinese soft-shelled turtles.
Article
Agriculture, Dairy & Animal Science
Naicheng Liu, Peng Zhang, Mingyang Xue, Zidong Xiao, Mengjie Zhang, Yan Meng, Yuding Fan, Junqiang Qiu, Qinghua Zhang, Yong Zhou
Summary: In this study, the bacterial community structure of Chinese soft-shelled turtles (SST) and its aquaculture water system were analyzed using Illumina MiSeq sequencing. The results showed that the bacterial diversity and richness compositions in the intestinal tract and aquaculture water were the same, but the relative abundances of bacterial communities varied. The intestinal microorganisms in the SSTs experienced significant changes after transfer to a different environment.
Article
Biology
Zhao Lv, Yazhou Hu, Jin Tan, Xiaoqing Wang, Xiaoyan Liu, Cong Zeng
Summary: The dominant disease in the aquaculture of Chinese soft-shelled turtle is hemorrhagic sepsis caused by Aeromonas hydrophila infection. This study identified two groups of turtles with different susceptibility to A. hydrophila and examined their gene expression profiles post-infection, providing new insights into immune mechanisms against A. hydrophila.
Article
Materials Science, Multidisciplinary
Li Yang, Yating Wei, Shuai Gao, Qifei Wang, Jiaqi Chen, Boping Tang, Xunguang Bian
Summary: Copper nanoparticles have a significant impact on the epididymis and sperm viability of Chinese soft-shelled turtles, while copper ions have a greater toxic effect.
Article
Agriculture, Dairy & Animal Science
Lu Zhou, Jinhong Lei, Xiaofei Zhai, Ningning Lu, Haitao Shi, Jichao Wang
Summary: Vocalization is crucial for the survival of underwater animals. The Chinese soft-shelled turtle, a highly vulnerable species, has not been studied for its underwater vocalization, which can provide important insights for ecological and evolutionary research. By conducting underwater recordings, we identified 10 call types of the turtle and observed differences in vocalizations between individuals of different ages and sexes. The Chinese soft-shelled turtle has a diverse range of vocalizations and shows the tendency for vocalization to become more diverse with age, aiding its adaptation to complex and dim underwater environments.
Article
Fisheries
Peng Zhang, Naicheng Liu, Zidong Xiao, Mengjie Zhang, Yan Meng, Yuding Fan, Xiaowei Hu, Junqiang Qiu, Qinghua Zhang, Yong Zhou
Summary: White abdominal disease is a major disease in Chinese soft-shelled turtle aquaculture. Pathological characteristics of diseased turtles were studied and showed changes in hematological, histopathological, intestinal microbial, and immune gene expression. The diseased turtles exhibited anemia, visceral tissue lesions, reduction of intestinal microbial species, and innate immune activation.
AQUACULTURE REPORTS
(2023)
Article
Chemistry, Applied
Fen Wang, Zhu Chen, Yunsheng Cheng, Guanjun Hou, Suofei Ji, Ye Zhang, Jie Li, Chengjun Zhu, Yuchun Wu, Guangtong Song, Yelin Jiang
Summary: The study found that the muscle of Yangtze and Japanese strains of Chinese soft-shelled turtle is high in protein and low in fat, making it beneficial for human health, but caution should be exercised when consuming the calipash.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2021)
Article
Fisheries
Yuqi Mu, Mengyan Lan, Yali Li, Zhao Zhang, Yueqiang Guan
Summary: Florfenicol has significant effects on the antioxidant and immune systems of the Chinese soft-shelled turtle (Pelodiscus sinensis). Various dosage levels of florfenicol were found to influence antioxidant enzyme activities and immune-related gene expression. The study provides insights into the molecular mechanisms and pathways involved in the florfenicol response of P. sinensis.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Pei Wang, Gang Xiong, Dan Zeng, Jianguo Zhang, Lingrui Ge, Li Liu, Xiaoqing Wang, Yazhou Hu
Summary: MicroRNAs (miRNAs) play important regulatory roles in pigmentation processes in aquatic animals. In this study, we performed an integrated analysis of miRNA and mRNA expression profiles in the skin of Chinese soft-shelled turtles during three embryonic stages. We identified novel genes and miRNAs, and found differentially expressed genes (DEGs) and miRNAs (DEMs) between different stages. Some of these DEGs and DEMs may be associated with turtle pigmentation in embryonic development. Our findings provide insights into the regulatory patterns of miRNAs in reptile pigmentation and may contribute to understanding the molecular processes involved in pigmentation.
Article
Marine & Freshwater Biology
Rui Feng, Zhao Zhang, Yueqiang Guan
Summary: Nitrite affects the oxygen-carrying capacity of blood, liver antioxidant system, and lipid peroxidation in Chinese soft-shelled turtles, while also triggering immune responses. The study identified differentially expressed genes and pathways involved in cellular responses to nitrite stress.
AQUATIC TOXICOLOGY
(2021)
Article
Environmental Sciences
Yufei Huang, Sheng Yang, Xuebing Bai, Yonghong Shi, Qiusheng Chen
Summary: The Chinese soft-shelled turtle is one of the most primitive amphibians and reptiles, attracting attention due to its environmental suitability and unique hibernation habits. Research on the molecular mechanisms underlying the periodic variation of lipid droplets in the liver of this species revealed a shift in energy metabolism during hibernation, with lipid oxidation becoming the main mode of energy generation. Further analysis showed that lipolysis plays a key role in the degradation of hepatic lipid droplets during hibernation in the Chinese soft-shelled turtle.
FRONTIERS IN MARINE SCIENCE
(2021)
Article
Reproductive Biology
Bangsai Yang, Wei Sun, Lin Jin, Pan Li, Yingjie Zhou, Guoying Qian, Chutian Ge, Ling Xiao
Summary: SOX8 is found to be a key regulator of early male differentiation in Chinese soft-shelled turtles, as it exhibits male-specific higher expression during the critical period of sex determination and is rapidly down-regulated during male-to-female sex reversal. Knockdown of SOX8 leads to complete feminization, highlighting its crucial role in reptile sex determination.
BIOLOGY OF REPRODUCTION
(2023)
Article
Environmental Sciences
Jing Xiang, Hao Wu, Jinwei Gao, Weimin Jiang, Xing Tian, Zhonggui Xie, Tao Zhang, Jia Feng, Rui Song
Summary: Niclosamide (NIC) is a molluscicide used for controlling schistosomiasis, but its negative effects on aquatic animals, such as Chinese soft-shelled turtles, have been reported. This study investigated the toxicity mechanism of NIC on Chinese soft-shelled turtles and found that NIC exposure induced severe liver damage and increased ROS production. The activities of antioxidant enzymes were inhibited, indicating a disruption in the antioxidant defense. Transcriptome sequencing identified the disorder of lipid metabolism and innate immune regulation as toxicological properties of NIC. Overall, this study provides valuable information on the toxic effect of NIC on Chinese soft-shelled turtles.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Fisheries
Junxian Zhu, Luo Lei, Chen Chen, Jiansong Li, Yongchang Wang, Congcong Wu, Yakun Wang, Xiaoyou Hong, Xiaoli Liu, Lingyun Yu, Haigang Chen, Chengqing Wei, Yihui Liu, Wei Li, Xinping Zhu
Summary: In this study, a medium-free incubation method for hatching Pelodiscus sinensis eggs was developed and compared with the traditional vermiculite incubation method. The results showed that medium-free incubation did not significantly affect hatching success, body weight, carapace length, carapace width, plastron length, plastron width, and righting response time of P. sinensis compared to the traditional vermiculite incubation. Furthermore, light treatment shortened the incubation period and had negative effects on certain parameters during medium-free incubation. This study also discovered the phenomenon of egg-hatching synchrony in P. sinensis.
AQUACULTURE REPORTS
(2023)
