Journal
ANIMAL REPRODUCTION SCIENCE
Volume 103, Issue 1-2, Pages 187-192Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.anireprosci.2007.04.006
Keywords
oocyte maturation; epiregulin; amphiregulin; phosphodiesterase; equine
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Human chorionic gonadotropin (hCG) was administered to mares in estrus with large, dominant ovarian follicles to initiate follicular and oocyte maturation. Follicular contents were collected at 0, 2, 4 and 6 h after hCG. Epiregulin, amphiregulin and phosphodiesterase (PDE) mRNA contents of granulosa cells (PDE 4D) were determined by reverse transcription and real-time PCR; PDE 3A mRNA content of single oocytes was determined similarly. Copy numbers of mRNA did not increase for PDE 3A or 4D over the time interval studied. Amounts of ephiregulin and amphiregulin mRNA were correlated (r=0.98) when log transformed. Epiregulin and amphiregulin mRNA increased (P< 0.01) from controls by 4h after hCG administration, with amphiregulin increasing (P < 0.01) by 2h after hCG administration. Epiregulin and amphiregulin mRNA levels remained elevated (P < 0.01) at 6 h after hCG. These results indicate that EGF-like growth factors are likely paracrine mediators of the LH signal in the horse. (C) 2007 Elsevier B.V. All rights reserved.
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